7/31/2019 AniOvul by Dairy Master
1/1
GENERAL
LH (Luteinizing Hormone) is one kind ofglycoprotein hormone.
Just like FSH (Follicle-Stimulating Hormone), its one of the main
factorsmotivating the ovulation. In female mammals, LH
iscooperating with FSH to aid the follicle growingmaturely and
motivate the ovulation. In addition, itenhances the blood flow.
Ovulation is the process inwhich the ovary releasing the ovum and
its the keyevent in ovary period. The ovulation of mammals isa
complex process involved with many genes and
steps. A lot of research indicates that ovulation isregulated by
hormone and partial active substanceof follicle. Several or more
hours before theovulation of mammals, secretion of LH would rise
inresponse to female hormone positive regulation andLH surge
appears. After the LH surge, the wall ofthe enlarged follicle
ruptures at ovulation and themature ovum is extruded. After
ovulation, LHreturns to its base line level with the
concomitantincrease of progesterone level to initiate lutealphase.
Before ovulation, the LH in urine, bloodserum and milk rises
gradually and the ovulationoccurs approximately 12-14 hours after
the LHsurge.
In view of the characteristic variation of LH, rapidand
sensitive measurement of LH is an importanttool in the diagnosis
and management of infertilityin female mammals.
HOW TO USE
As the cow come into heat, perform this test afterten hours for
normal cows which shows heat inproper cycle. If found positive,
breed that cow withinterval of 12 hours. Incase of negative
result,perform this test again with the interval of 5 to 6
hours and breed at positive result.For disturbed heat cycle
cows, perform this testafter 12 to 14 hours later to start of heat.
If foundpositive, breed that cow with interval of 12 hours.Incase
of negative result, perform this test againwith the interval of 5
to 6 hours depending upon
the heat cycle history of cow. It is important thcow should be
breed twelve hours later to positresult.
SPECI MEN COLLECTI ON AN D PREPARATI ONTake the urine sample as
it is as or take bloocentrifuge it and separate blood serum as
sampfor this test.
ASSAY PROCEDURE
1. Remove the test device and dropper from its fwrapper by
tearing along the slice.
2. Using the dropper, withdraw the urine or serusample from the
specimen cup and slowdispense 3-4 drops into the circular sample
weWhen the sample liquid moves slowly to tother end due to the
capillarity, it will dissolthe solid gold labeled antibody and
broughtahead in the mean time.
3. Read results within 10 minutes.I NTERPRETATI ON OF
RESULTSPositive: If the test band (T) is more intensthan or
equivalent to that of control band (C), tspecimen is likely at
surge level.
Negative: If only the control band (C) appears, the color
intensity of the Test line is less than thof Control line, this
means the specimen is belowthe surge level.I nvalid: When neither
test nor control baappears on the membrane, the test should voided
since improper test procedure deterioration of reagents probably
occurred. It recommended that the test be repeated.
PRECAUTI ON1. Read directions for use carefully befo
performing this test.
2. Do not uses test kit beyond expiration date.3. Do not reuse
the test devices and discard it in
the dustbin after single use.4. Once opened, the test device
should be us
immediately. Prolonged exposure to atmosphewill lead to
deterioration.
AANNIIOOVVUULLCATTLE OVULATION TEST KIT
DDaaiirryy MMaasstteerrss Cont act . +92 300 4570089Plot N um
602-G block, Sabzazar Scheme, E mail I D . [email protected] ult
an Road, L ahore
