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ifferent types of molecular marker systems can be used in theelection of resistant genotypes. In this work, stripe rust resis-ant genotypes (Izgi2001, Sönmez2001, PI178383) and susceptibleenotypes (Aytın98, ES14, Harmankaya99) were used as parents.ccordingly, PI178383 × Harmankaya99, Izgi2001 × ES14, Sön-ez2001 × Aytın98, PI178383 × Aytın98 and Izgi2001 × Aytın98
ombinations have been created and F2 individuals were obtained.ulk segregant analyses were performed on resistant and sus-eptible parents and their F2 individuals by using 366 SSR, 190ST-SSR, 58 ISSR, 96 RGAP, 18 SRAP, 34 AFLP, 124 RAPD, 17 STS,09 EST derived primers (39 contigs, 92 singletone, 78 RGAP) tond out molecular marker/markers genetically linked with stripeust disease resistance source. As a result of screening studies,eing specified to the different combinations, totally 6 DNA mark-rs genetically linked with stripe rust disease resistance sourceere identified. These were namely 3 SSR (Xgwm382, Xgwm311,wmc658), 2 EST-SSR (PK54, BU099658) and 1 AFLP (P-GAC/M-
CG). On the other hand, the level of genetic diversity of theseesistant and susceptible wheat genotypes was measured and com-ared by several types of PCR-based markers, the highest/lowestimilarity and PIC values of the loci were calculated.
Keywords: Stripe rust; DNA marker; Triticum aestivum L.;arker assisted selection; Genetic diversity
ttp://dx.doi.org/10.1016/j.nbt.2012.08.385
oster 3.5.19
n efficient propagation system via somatic embryogen-sis
urcu Cetin
Dumlupınar University, Kütahya, Turkey
he rapid and significant improvements in the somatic embryo-enesis methods allow extensive practical and commercialpplications, particularly for in vitro clonal micropropagation.e used two-step direct somatic embryogenesis for the mass
ropagation of Black Carrots. The callus were derived fromypocotyl of aseptic seedlings when transferred to Murashige andkoog (1962) (MS) solid medium containing 0.1–0.5–1 mg L−1, 2,4-ichlorophenoxyacetic acid (2,4-D), 30 g L−1 of sucrose and 8 g L−1
f agar for two weeks. The best callus initiation was obtained with.5 mg L−1, 2,4-D. The callus were placed on MS solid mediumithout growth regulator. The regenerated shoots and somatic
mbryos were developed to plantlets in this medium. The result
f our study shows that two-step direct somatic embryogenesis isuseful method for the mass propagation of Black Carrots.ttp://dx.doi.org/10.1016/j.nbt.2012.08.386
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New Biotechnology · Volume 29S · September 2012
oster 3.5.20
he effect of magnetic field on in vitro seed germination,eedling growth and shoot regeneration from cotyledonode explants of Lathyrus chrysanthus Boiss
ansu Telci Kahramanogullari 1,∗, Ramazan Beyaz2, Behrouzlizadeh3, Mustafa Yildiz1
University of Ankara, Faculty of Agriculture, Department of Fieldrops, 06110 Dıskapı/Ankara, TurkeyUniversity of Ankara, Biotechnology Institute, Gölbası/Ankara, TurkeyUniversity of Ankara, Graduate School of Natural and Applied Sci-nces, Department of Field Crops, Gölbası/Ankara, Turkey
lthough there are many reports on the effects of magnetic fieldreatments on seed germination, plant growth, protein biosyn-hesis and root development, to our knowledge this is the firsttudy indicating the effect of magnetic field on in vitro seed ger-ination, seedling growth and shoot regeneration capacity of
otyledon node explants in Lathyrus chrysanthus Boiss. Lathyrushrysanthus seeds of an ecotype (Diyarbakir) found in southeastf Turkey were subjected to magnetic field strength 125 mT for 0,4, 48 and 72 hours. Then seeds were surface sterilized using 75%ommercial bleach (containing 5% sodium hypochlorite) at 35 ◦Cor 15 min with continuous stirring and were then rinsed threeimes with sdH2O at the same temperature. Sterilized seeds werehaked in sdH2O for 6 h to increase the permeability of seed coatnd then were germinated on a basal medium of Murashige andkoog’s (MS) mineral salts and vitamins, 3% sucrose, and 0.7%gar in Magenta vessels. All cultures were incubated at 15 ± 1 ◦C inark for 5 days, then they were transferred to growth chamber at4 ± 1 ◦C under cool white fluorescent light (27 �mol m−2 s−1) with16 h light/8 h dark photoperiod. Seed germination and seedlingrowth percentages were recorded 5 and 14 days after culture initi-tion, whereas seedling and root lengths were noted 28 days after.otyledon node explants were excised from 28-day-old seedlings.he highest results regarding in vitro seed germination and seedlingrowth, and regeneration capacity of cotyledon node explantsere recorded from 24 h magnetic field treatment.
Keywords: Lathyrus chrysanthus Boiss.; Magnetic field;eedling growth; Shoot regeneration
ttp://dx.doi.org/10.1016/j.nbt.2012.08.387
oster 3.5.21
arker assisted selection for photoperiod insensitivepd-D1a allele in winter wheat breeding program
ayati Akman1,∗, Phil Bruckner2
Selcuk University, Sarayönü Vocational School of Higher Education,2430 Konya, TurkeyMontana State University, College of Agriculture, Department of Plantciences and Pathology, MT 59717, Bozeman, USA
his study was conducted in Spring Wheat Lab of Montana State
niversity. Marker assisted selection is a useful molecular breedingethod to improve the efficiency and precision of conventionallant breeding. The Ppd-D1a allele of 49 lines in F2 generationnd the 4 parents Yellowstone, NE01533, Pelsart and Promontory
