AGTC Bioproducts Short Price list

Short Form Catalogue and Price List 2010

GLOBAL LIFE SCIENCE SUPPLY

Contact Us:

AGTC Bioproducts Ltd t/a National DiagnosticsTel: 01482 646020Fax: 01482 646013Email: [email protected] 4 Fleet Business Park, Itlings Lane, Hessle, Hull, HU13 9LX

Page No

Electrophoresis

Transfection

Peptide/Organic Synthesis Reagents

Microbiology Media

Electro-Optical Cleaning Agents

Scintillation Counting

Histology

Electrophoresis Agaroses

Dithiothreitol

Terms & Conditions

Plasmid Purification

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19

18

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10

7

6

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24

20

CONTENTS

NATIONAL DIAGNOSTICS Electrophoresis

www.agtcbioproducts.com

With the introduction of ProtoGel Quick-Cast, the time required for casting gels for protein separation has been

reduced from 2 hours to under 25 minutes, and the number of steps involved has been cut from 10 to only

3. With ProtoGel Quick-Cast, simply measure out the amount needed, add initiators and cast the gel. No

mixing, no multiple measurements, no need for a second casting to produce a stacking gel. Just initiate,

cast, and be ready to run in 20 minutes. With the reduced casting time, western blots can be finished

within a regular working day.(NDH)

· Cast a Gel in One Fourth the Time· Ideal for Western Blotting Applications· Precast Convenience without the Precast Price

ProtoGel Quick-Cast 12%®

Featured Products

1

Product Name Description Code Pack Size £

Diogenes Enhances superoxide detection. CL-202 1 Kit £178.20

Hydrogen Peroxide Assay Kit Cl-204 1 Kit £86.77

HRPL KitFor enhanced non-isotopic detection of proteins and nucleic acids inWestern and Southern blot techniques.

CL-205 1 Kit 240 ml £428.27

CL-205 1 Kit 900 ml £140.90

ProtoBlock SystemTo enhance signal to noise ratio during chemiluminescent membranebound visualization of Proteins and DNA.

CL-252 1 system £30.89

AcrylamideNational Diagnostics' Acrylamide has been specially purified to produceconsistent gel matrices for the most demanding requirements.

EC-201 100 grams £21.88

EC-201 500 grams £70.76

EC-201 1 kilogram £124.17

Bis (Methylene Bis-Acrylamide)

BIS is a crosslinking agent for general acrylamide electrophoretic use.Specially purified.

EC-301 25 grams £13.79

DATDDATD is an allyl type crosslinker containing a 1,2-diol structure which maybe oxidized by periodic acid.

EC-303 25 grams £31.55

Glycine Purified by novel chelation chromatography to remove all reactive metals. EC-405250 grams £9.65

1 kilogram £25.73

TrisBase component for PAGE buffer formulation. Purified to removeammonia and amine contaminants. Results in greater resolution duringisoelectric focusing.

EC-406250 grams £12.99

1 kilogram £34.74

TricineUltra pure molecular biology grade reagent used as a base component forprotein PAGE buffer formulation.

EC-407 100 grams £34.74

Ion Exchange ResinA mixture of anionic and cationic resins specially prepared for thedeionization of electrophoretic gel monomer solutions prior to initiation.

EC-408 100 grams £19.31

RiboflavinFor the photo-polymerization of polayacrylamide electrophoresis gels.Single crystalline species ensures greater reproducibility.

EC-501 25 grams £19.31

TEMEDFractionally distilled to remove all trace metals and amine impurities,which inhibit gel formation.

EC-503 25 ml £12.99

Ammonium PersulfateExceeds ACS Standards. Low absorbed water results in consistentinitiating capabilities.

EC-50425 grams £19.31

100 grams £19.31

Dithiothreitol (DTT)Cleland's Reagent, a reducing agent used in protein chemistry. Speciallypurified to remove trace metals and other impurities. Low odourformulation. Kilo price on request.

EC-601

1 gram £11.57

5 grams £71.85

25 grams £85.50

50 grams £101.18

100grams £118.97

2-MercaptoethanolTriple distilled to remove impurities and stored under nitrogen to preventtheir reformation.

EC-603 50 ml £21.88

SDSPurified to remove colored contaminants that interfere withspectrophotometric analysis.. Zero free sulfates eliminate non-specificbinding.

EC-604100 grams £19.31

1 kilogram £85.57

UreaRecrystallized to remove ammonia. Exclusive purification process hasbeen shown to inhibit ammonia regeneration.

EC-605250 grams £14.80

1 kilogram £31.54

NATIONAL DIAGNOSTICSElectrophoresis

Phone: 01482 646020 Fax: 01482 646013 Email: [email protected]

ND Protein Precipitation Kit NEW Recovery of All Proteins from Complex Mixtures Precipitates as Little as 100ng of BSA at 0.25µg/ml Milder than TCA More Reliable than Acetonitrile or Ammonium Sulfate

The ND Protein Precipitation Kit is mild on samples and easy to use. It casts the finest net of anyprocedure, allowing the high yield collection of all proteins in solution, precipitating even the most diluteproteins. The ND Protein Precipitation Kit allows collection of proteins that would be missed by othermethods .Ideal for both routine and difficult work, the ND Protein Precipitation Kit stands alone amongall methods. It offers the best combination of mildness, simplicity and effectiveness in protein precipitation.

2


GlycerolElectrophoresis grade, for use in loading buffers and to alter the mechanicalproperties of gels.

EC-606 450 ml £28.59

Tween 20Specially purified to remove epoxidized components. Nonionic surfactant usedin Western blotting and other protein studies.

EC-6071 ltr £37.95

200ml £25.99

Formamide Redistilled for absolute purity. DNA denaturing agent. EC-608 200 ml £33.45

EC-615-25ML Formamide ULTRA CE, Specialy purified for caplillary electrophoresis EC-615 25 ml 18.73

EDTAComponent of electrophoresis buffers. Ultra pure, molecular biology gradereagent.

EC-610 100 grams £50.18

Glass BondNational Diagnostics' Glass Bond allows polyacrylamide gels to be temporarilyaffixed to one of the glass plates used for casting electrophoresis gels.

EC-620 25 ml £71.29

Glass FreeCoats glass plates to prevent binding of polyacrylamide gels, allowingConvenient disassembly of the cassette after electrophoresis.

EC-621 450 ml £35.64

GelDry Film 11 X 12 cmGelDry Film is a specially designed drying film to be used in air-dryingprocedures for polyacrylamide gels.

EC-612 50 sheets £23.79

Sterling Rapid Silver StainThe fastest and most sensitive silver stain to date, the Sterling Rapid SilverStain Kit produces superb results in one hour.

EC-720 1 kit £99.72

ProtoBlue Safe The Best Performing Colloidal Stain - Detects as little as 5ng protein EC-7221 liter £59.35

4 liter £193.66

NuclistainNuclistain visualizes in the visible spectrum and is less toxic than ethidiumbromide. Detects as little as 50ng of DNA.

EC-730 25 ml £26.17

Accugel PAGE DNA Extraction Kit (50 PREPS) EC-745 KIT £60.12

Protprep KIT II Meltable acylamide matrix EC-755 KIT £102.94

Insite SystemIn-gel fluorescent stain for proteins. The fastest high sensitivity detection ofproteins following SDS-PAGE available.

EC-759 1 Kit £115.81

AcrylaGel 30% ultra-pure stock solution of acrylamide. EC-810450 ml £23.16

1 liter £30.24

Bis-AcrylaGel 2% ultra-pure stock solution of bisacrylamide. EC-820450 ml £17.38

1 liter 23.16

SequaGel Concentrate Monomer concentrate component of the SequaGel Sequencing System.EC-830 450 ml £23.80

EC-830 1 liter £45.03

SequaGel Sequencing SystemSystem to conveniently formulate 19:1 acrylamide/bisacrylamide sequencinggels up to 20%.

EC-833 1 liter kit £74.62

equaGel Buffer Buffer component of the SequaGel Sequencing System. EC-835 100 ml £12.87

SequaGel-6Convenient systems with 19:1 monomer, urea, TBE, and TEMED for casting 6percent denaturing gels.

EC-836450 ml £22.53

1 liter £34.74

SequaGel-8Convenient systems with 19:1 monomer, urea, TBE, and TEMED for casting 8percent denaturing gels.

EC-838 450 ml £21.23

EC-838 1 liter £45.03

SequaGel TTE CompleteBuffer

Order separately to run glycerol tolerant gels with SequaGel 6 or 8. EC-839 200 ml £23.14

SequaGel Diluent Diluent component of the SequaGel Sequencing System. EC-840450 ml £11.57

1 liter £23.16

NATIONAL DIAGNOSTICS Electrophoresis


ProtoBlue Safe The Best Performing Colloidal Stain - Detects as little as 5ng protein� ProtoBlue Safe improves both the sensitivity and the universality of staining. ProtoBlue Safe

is less prone to high background caused by trace residual SDS in the gel. Costs Less than Regular Coomassie� Laboratories typically spend twice as much per gel in the methanol and acetic acid for staining

and destaining than for ProtoBlue Safe (including ethanol cost). In addition, ProtoBlue Safeis much faster, more sensitive, and you can pour the used stain solution down the drain. Protein visualization is faster, safer,more sensitive and less-expensive with ProtoBlue Safe. Non hazardous Disposal.

3


SequaGel Complete BufferComponent of SequaGel 4, 4.25, 4.75, 6 or 8. No need to orderseparately.

EC-841 200 ml £11.57

SequaGel XRProprietary formulation for extended reads in both manual sequencegels and automated sequencers.

EC-842450 ml £25.73

1 liter £45.03

SequaGel XR ConcentrateProprietary formulation for extended reads in both manual sequencegels and automated sequencers.

EC-843100 ml £41.81

450 ml £115.80

SequaGel MD Monomer SolutionProprietary solution for the resolution of minor mutational differencesin conformational analysis.

EC-845 200 ml £86.20

SequaGel MD SSCP KitProprietary solution for the resolution of minor mutational differencesin conformational analysis. Contains matrix concentrate and SSCP StopSolution.

EC-846 1 kit £89.41

SequaGel MD Heteroduplex KitProprietary solution for the resolution of minor mutational differencesin conformational analysis. Contains matrix concentrate and Triple DyeLoading Buffer.

EC-847 1 kit £89.41

SequaGel MD SSCP Stop Solution Formamide loading buffer. EC-848 1.2 ml £10.30

AccuGel 19:1 (40%) 40% stock molecular biology grade solution of acrylamide/bisacrylamide, 19:1 ratio. EC-850

450 ml £30.24

1 1iter £46.32

AccuGel 29:1 (30%)30% stock molecular biology grade solution of acrylamide/bisacrylamide, 29:1 ratio. EC-851

450 ml £30.24

1 1iter £46.32

AccuGel 29:1 (40%)40% stock molecular biology grade solution of acrylamide/bisacrylamide, 29:1 ratio. EC-852

450 ml £30.24

1 liter £46.32

Triple Dye Loading Buffer (6X)Three dyes (Xylene Cyanol, Bromophenol Blue, and Orange G) allowcontinuous monitoring of electrophoretic runs.

EC-855 1.2 ml £16.09

TBE 10X Commonly used gel and tank buffer for DNA electrophoresis. EC-8601 liter £21.23

4 liters £47.61

TBE 5X Commonly used gel and tank buffer for DNA electrophoresis. EC-861 4 liter £25.73

TE BUFFER 100XConcentrated solution of 1 M Tris-HCl, pH 8, with 100 mM EDTA, thestandard for DNA and RNA purification and storage.

EC-862 25 ml £33.45

Tris-Tricine-SDS PAGE Buffer (10X)SDS-PAGE tank buffer specifically designed to improve the resolutionof small proteins (5 - 20 kD)

EC-869 1 liter £82.36

Tris-Glycine-SDS PAGE Buffer (10X)Ultra-pure, standardized Laemmli running buffer for SDS PAGE ofproteins.

EC-8701 liter £20.58

4 liter £38.60

TTE Glycerol Tolerant Buffer 20XFor making glycerol tolerant PAGE gels, this buffer must be used withProtoPrep for DNA electrophoresis.

EC-871 1 liter £34.74

TAE Buffer 50XUltra-pure preformulated buffer for electrophoresis when samplerecovery is important.

EC-872 1 liter £36.03

SSC Buffer 20XNational Diagnostics SSC Buffer (20X) is optimized for Northern orSouthern Capillary Blotting.

EC-8731 liter £19.40

4 liters £48.26

NATIONAL DIAGNOSTICSElectrophoresis


Stringent Quality Control - 18 Mega ohm Water - 0.2 Micron Filtration - Save Money - Save Time - Improve Results

In the gel and in the tank, the purity and strength of buffers determine not only the electricalparameters of electrophoresis but also the structure and solubility of the bio-molecules under study.Buffer quality is as important for electrophoresis results as the gel matrix itself. Produced to themost exacting standards in the industry, National Diagnostics’ buffers are stable and pure. Theyeliminate weighing and mixing, save time, and help ensure reproducible results.

Buffer Solutions

� Tris-Glycine Electroblotting Buffer (10X)� TBE Buffer (10X or 5X)� Triple Dye Loading Buffer (6X)� Capillary Electrophoresis Buffer (10X)� Tris-Glycine-SDS PAGE Buffer (10X)� Tris-Trycine-SDS PAGE Buffer (10X)� ProtoGel Buffers

4


SDS Solution 20%National Diagnostics SDS Solution 20% saves time, is more stable andprovides more reproducibility than making up this solution in the lab.

EC-874100 ml £16.09

450 ml £33.45

Denaturation SolutionReady-to-use, ultra-pure solution of NaCl and NaOH for Southern andNorthern Blotting, and in situ hybridization procedures.

EC-875 1 liter £15.44

Neutralization Solution Returns gels to pH 7 after denaturation, prior to Southern capillary blotting. EC-876 1 liter £15.44

Dextran SulfateAids in formation of networks (high localized concentrations of probes)during hybridization, thus expediting the annealing process.

EC-877 50 grams £75.27

Tris-Glycine ElectroblottingBuffer 10X

Optimized for electrophoretic transfer of proteins to PVDF, Nitrocellulose orNylon membranes in Western Blotting.

EC-8801 liter £16.72

4 liter £51.47

Protein Loading Buffer Blue 2XReady to use buffer solution with bromophenol blue for the preparation ofprotein samples to be separated in SDS-PAGE.

EC-886 10x1 ml £18.66

Protein Precipitation Kit

ND Protein Precipitation Kit

· Recovery of All Proteins from Complex Mixtures· Precipitates as Little as 100ng of BSA at 0.25µg/ml· Milder than TCA· More Reliable than Acetonitrile or Ammonium Sulfate

EC-8881 kit

(50 ml) £73.63

ProtoGel Stabilized, 30% acrylamide/bis solution (37.5:1 ratio) optimized for SDS-PAGE of proteins.

EC-890450 ml £36.66

1 liter £61.12

ProtoGel 40%Stabilized, 40% acrylamide/bis solution (37.5:1 ratio) optimized for SDS-PAGE of proteins.

EC-891

450 ml £46.40

1 liter £77.33

ProtoGel Resolving BufferReady-to-use buffer to formulate standard Laemmli resolving gels of .375 MTris-HCl and 0.1% SDS, pH 8.8.

EC-892450 ml £19.31

1 liter 28.94

ProtoGel Stacking BufferReady-to-use buffer to formulate standard Laemmli stacking gels of .125 MTris-HCl and 0.1% SDS, pH 6.8.

EC-893 200 ml £15.44

ProtoGel QC 12%Ready-to-use gel solution for SDS-PAGE that combines the convenience ofpre-cast gels with the economy of casting your own.

EC-895100 ml

£16.53

450 ml £57.49

Insite MarkersPrestained markers combined with unstained, engineered standards. Idealmarkers for fluorescent detection methods.

EC-897 0.5 ml£77.20

ProtoMarkersPrestained protein markers for Western Blot and SDS-PAGE combining high-contrast visualization and easy orientation.

EC-898 0.5 ml £94.56

ProtoMetrics Non-stained, engineered protein markers EC-899 1 Tube £115.80

� TAE Buffer (50X)� TTE Glycerol Tolerant Buffer (20X)� Protein Loading Buffer Blue (2X)� TE Buffer (100X)� Neutralization Solution� Denaturation Solution� SSC Buffer (20X)


AGTC Bioproducts Electrophoresis Agarose

STANDARD (ROUTINE) AGAROSENEW AND IMPROVED

Hi-Res Standard (EEO 0.13%)-D1Our new production method has produced a superb medium forseparating nucleic acid fragments < 23 Kb. This agarose is also suitablefor Blotting Assays, Presence of DNAses and RNAses not detected. Thereis no better value agarose than this.

Hi-Res Standard GQT (Genetic Quality Tested)-D1-GQT Highly purifiedagarose, with low levels of EEO, GQT (Genetic Quality Tested).Recommended for DNA preparatory gels. Presence of DNAses andRNAses not detected.

Product Code Size Price

AG-D2-25 25 gm £17.63

AG-D2-100 100 gm £60.31

AG-D2-500 500 gm £261.72

AG-D2-1k 2 x 500 gm £395.00

Hi-Gel Standard (D2)Recommended agarose for Agarose Beads preparation, it can also beused for protein electrophoresis and counter immunoelectrophoresis.Presence of DNAses and RNAses not detected.

LOW MELTING AND GELLING POINTS

Hi-RES Low Melt-LM2Recommended agarose for <1000 bp fragmentseparation in analytical and preparatoryelectrophoresis. The low melting point of this

agarose (65oC) allows the gel to melt withoutdamaging the DNA. Presence of DNAses and RNAsesnot detected.

Low Melt Mega Clone-LMGQT GQT (GeneticQuality Tested)Especially recommended for preparatoryelectrophoresis of nucleic acids >1.000 bp and In-Gelapplications.

Low Melt Clone-LMSVEVery low melting point agarose has a higherresolution capability for fragments <1.000 bp,especially for PCR products, between 200-800 bp.


AG-LM2-25 25 gm £76.56

AG-LM2-100 100 gm £242.19

AG-LM2-25-GQT 25 gm £86.85

AG-LM2-100-GQT 100 gm £346.19

AG-LMSVE-25 25 gm £59.49

AG-LMSVE-100 100 gm £196.30

MS (MOLECULAR SCREEN) HIGH RESOLUTION

Hi-Res Super + -MS4Recommended for analytical separation of DNA <500 bp. Presence ofDNAses or RNAses not detected, no DNA binding detected.

Hi-Res Super-MS8Recommended for analytical separation of DNA < 1000 bp particularlyuseful for PCR products. Presence of DNAses or RNAses not detected, noDNA binding detected.

Hi-Strength Analytical - MS12Recommended for analytical separation of DNA. At 2% concentration itcan separate fragments of 100-1500 bp. Presence of DNAses or RNAses,no DNA bindingdetected.

Mega-Base Rapid (High Strength Gel)-(D5)Specific agarose for pulsed fields electrophoresis,recommended for DNA > 1000 bp separation, byconventional electrophoresis and for DNA with a size ofseveral megabases by ”Pulse Field Gel Electrophoresis”.Presence of DNAses and RNAses not detected.


AG-D1-25 25 gm £17.63

AG-D1-100 100 gm £60.31

AG-D1-500 500gm £261.72

AG-D1-1k 2 x 500gm £380.69

AG-D1-25-GQT 25 gm £18.50

AG-D1-100-GQT 100 gm £63.00

AG-D1-500-GQT 500gm £270.00

AG-D1-1K-GQT 2 x 500gm £410.00


AG-D5-25 25 gm £52.29

AG-D5-100 100 gm 179.51


AG-MS4-50 50gm £90.00

AG-MS4-100 100 gm £174.88

AG-MS4-250 250 gm £451.00

AG-MS8-25 25 gm £51.41

AG-MS8-100 100 gm £174.88

AG-MS8-500 500gm £639.56

AG-MS12-25 25 gm £90.00

AG-MS12-100 100 gm £239.83

AG-MS12-500 500gm £639.56

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NATIONAL DIAGNOSTICSHistology

The use of Histo-Clear in the laboratory means no longer having to breathe xylene when preparing histological sections. Distilled

from oranges, carefully purified, and stabilized, Histo-Clear is one of the safest clearing agents available. Histo-Clear not only

improves safety but also results. Histo-Clear leaves tissue less hard and brittle than xylene, facilitating the cutting of thin sections

and pro‐ longing microtome blade life. Nuclear morphology is rendered in fine detail. Histo‐Clear enhances the clarity and vibrancy

of acidophilic stains and improves staining of Harris’ Hematoxylin with a brighter Eosin background. Histo-Clear can directly

substitute for xylene and yields excellent results in automated tissue processing.

� Rapid Clearing without the Toxic Hazards of Xylene

� Biodegradable

� Greatly Reduces Disposal Costs

� Superior Results with Lower Citrus Odour

� Natural Product with Food Grade Rating World-Wide

Histo-Clear®

6


Histosol

High Flash point solvent replacement for xylene as a histological clearingagent. Completely miscible with all commonly used histology reagentsincluding Ethanol, Isopropanol, t-butanol and all embedding and mountingmedia.

HS-1001 gallon £14.28

5 gallon £73.76

Mirsky's FixativeMirsky's fixative enhances tissue penetration for Immunohistochemistry andImmunocytology. Produces high definition rendering of tissue usingmicrowave fixation.

HS-102200 ml system £12.87

2 liter system £32.16

HistomountPermanent UV stabilized mounting medium for preparation of permanentmount museum quality slides. Neutral pH. Dispensing device included.

HS-103100 ml £9.78

450 ml £21.88

Calci-ClearFor high quality bone and tissue decalcification, utilizing chelating andsequestering agents to efficiently remove solubilized calcium. Ideal wheremaintenance of structural integrity is paramount.

HS-104

1 quart £11.19

1 gallon £34.36

Calci-Clear RapidFor very rapid, high quality bone and tissue decalcification. Tissue distortionand structure loss is minimized without loss of speed.

HS-1051 quart £9.78

1 gallon £34.36

HydromountHigh quality water based mounting medium ideal for temporary mounts,immunofluorescence, staining procedures and frozen sections.

HS-106 100 ml £24.71

NeutralinNeutralin converts hazardous aldehyde waste into a nonhazardous polymerand water.

HS-108 5 gallon pail £24.96

Histo-ClearA nontoxic (food grade), low flammability, biodegradable solvent, Histo-Clearsubstitutes for xylene as a histological clearing agent. Histo-Clear improvessafety and results.

HS-2001 gallon £32.12

5 gallon £153.46

Histo-Clear IIWith reduced citrus odor compared to citrus based clearing agents, Histo-Clear II is a low toxicity, reduced flammability, biodegradable solvent,substituting for xylene as a histological clearing agent.

HS-2021 gallon £21.42

5 gallon £96.36

Alcian BlueHistological stain used to differentiate acid and neutral mucopolysaccharidesand as a differential stain for amyloid tissue.

HS-504 25 grams £17.38

Biebrich ScarletBiebrich Scarlet can be used as a plasma stain instead of Acid Fuchsin inMasson� s trichrome.

HS-506 25 grams £11.33

Fast Green FCFRecommended as a substitute for Light Green SF yellowish in Masson� strichrome because it is less likely to fade. It may be substituted for Light Greenin many other procedures as well.

HS-516 25 grams £19.31

Basic FuchsinBasic Fuchsin is the main ingredient of Schiff� s reagent and can be used asstain for glycoproteins and mucopolysaccharide proteins in acidic pH systems.

HS-518 25 grams £16.72

Methylene Blue Sensitive stain specific for DNA and RNA. HS-525 25 grams £23.16

Amido Black 10B Stain for proteins in gels and on Western Blots. HS-601 25 grams £21.23

Bromocresol Green Tracking dye for DNA electrophoresis in agarose. HS-602 5 grams £22.25

Bromophenol Blue Tracking dye for DNA or protein electrophoresis. HS-603 10 grams £17.24

Coomassie Blue R-250 Sensitive stain for proteins on polyacrylamide gels. HS-604 10 grams £11.57

Coomassie Blue G-250 Stain for proteins on polyacrylamide gels. Assay for proteins in solution. HS-605 10 grams £11.57

Methyl Green Methyl Green in conjunction with Pyronin will differentiate RNA & DNA. HS-606 10 grams £21.23

Pyronin Y Pyronin in conjunction with Methyl Green will differentiate RNA & DNA. HS-607 5 grams £39.50

Xylene Cyanol FF Tracking dye for DNA electrophoresis. HS-608 25 grams £35.64

NATIONAL DIAGNOSTICS Scintillation Counting


Choosing a Scintillation Fluid for Your Sample or ApplicationSamples and

ApplicationsScint i l la t ion Fluids (green) = Biodegradable

b e s t g o o d

Monofluor

L i qu i s

c i nt

Monoflow

1

Monoflow

2

Monoflow

3

Monoflow

4

Monoflow

5

F i l tr on -X

S o l us o l

Solusc in

t X

R

Oxosol C 1 4

Ca rbama t e

Betafl uor

Ecoscint A

Ecosc int

H

Ecosc int

O

Ecoscint

Unisc in

t BD

Hydrofluo r

Ecoscint X

R

Ecoscint F

low

Ecosc int U

ltra

B i oSo l /

B i os c i n

t

7

For nearly 40 years National Diagnostics liquid scintillation cocktails have provided a stable basis for all your counting needs. Our

high quality modern formulated cocktails and secondary reagents such as tissue solubilizers and specialty chemicals meet the very

highest standards of performance; enhancing user safety and convenience. In addition our unique and detailed manual of

techniques can help you with even the most challenging liquid scintillation counting requirements.

Scintillation Counting NATIONAL DIAGNOSTICS


When National Diagnostics

laboratories set out to

create Ecoscint Ultra, our

aim was to create the best

cocktail available for

environmental sample

counting and urine

bioassay. We are proud to announce that not only have we succeeded in this goal, we have surpassed it, creating

the best all-around performing cocktail on the market for both large and small aqueous samples. Ecoscint Ultra

enables ultra-low level counts to be discriminated from background not only for large but also for small samples.

With large samples, Ecoscint Ultra delivers up to 30% 3H counting efficiency at maximum sample hold with very

low background levels. With small samples, Ecoscint Ultra yields 3H efficiency greater than 60%.10ml Ecoscint

Ultra holds 12ml H2O in a clear emulsion at 18Co. 10ml Ecoscint Ultra holds 10ml 0.5M NaCl at that temperature.

� The Best Performing Cocktail on the Market

� Biodegradable

� Ultra-High Sample Holding (1 to 1.2 Cocktail to Sample)

� Ultra-Low Background

� Unsurpassed Counting Efficiency

Ecoscint Ultra T M

8


HydrofluorHydrofluor is a high efficiency, nonflammable liquid scintillation solution designedto count large volumes of aqueous samples. Hydrofluor offers a gel phase optionfor counting particulate samples.

LS-111 4 Gal £39.12

LiquiscintLiquiscint is an economical, ready-to-use liquid scintillation solution designed foraqueous and organic samples, a good all around performer.

LS-1211 Gal £31.00

5 Gal £149.14

Betafluor Betafluor provides high efficiency counting of non-aqueous samples. LS-1511 Gal £23.68

5 Gal £88.14

MonofluorMonofluor provides extremely high sample holding capacity, accomplishing aclear continuous liquid phase up to 50% sample. Monofluor utilizes anonflammable solvent base.

LS-191 4 liter £57.64

Filtron-XA scintillation fluid which solubilizes cellulose acetate, cellulose nitrate and mixedester filter disks assuring homogeneous counting.

LS-201 4 liter £54.18

Oxosol C14Oxidizer

Oxosol C14 is a complete scintillator designed to absorb and count 14CO2produced by sample combustion.

LS-211 1 gallon £88.13

Oxosol 306Oxosol 306 is a complete scintillation solution specifically formulated to count14CO2 samples trapped in Carbamate.

LS-231 1 liter £88.14

Carbamate-1 CO2Absorber

Carbamate-1 is a high capacity 14CO2 absorber intended to be used inconjunction with Oxosol 306.

LS-241 450 ml £200.00

Ecoscint Ultra LS-270 4 liter £68.87

EcoscintAn excellent all around performer, Ecoscint is biodegradable and nonflammable,with comparable efficiency and greater sample holding capacity thanconventional scintillators.

LS-2714 liter bottle £64.34

20 liter drum £194.17

Ecoscint XREcoscint XR is the best performing ultra-high sample hold fluid available. Ideal forenvironmental testing or any application requiring very high sample volume.


4x4 liter case £320.00

Ecoscint AReadily biodegradable with high flash point, low odor and low toxicity, Ecoscint Acombines high sample hold with high efficiency.



Ecoscint OFor nonaqueous samples, Ecoscint O exhibits lower backgrounds and improvedquench resistance. Ecoscint O is readily biodegradable with high flash point, lowodor and low toxicity.



Ecoscint HEcoscint H provides the maximum counting efficiency for aqueous samplevolumes under 10%. Ecoscint H is readily biodegradable with high flash point, lowodor and low toxicity.


20 liter drum£271.87

Uniscint BDUniscint BD is a biodegradable scintillation solution especially formulated toaccommodate high salt and buffer samples.

LS-276 4 liter bottle £65.22

NATIONAL DIAGNOSTICS Scintillation Counting


Formulated for multipurpose flow

applications, Ecoscint Flow represents the

utilization of National Diagnostics’ new

advances in scintillation fluid chemistry to

create a low viscosity cocktail. Ecoscint

Flow accepts a wide range of HPLC

gradients at a 1:1 ratio, providing high

counting efficiency (25% H3 efficiency at

1:1 cocktail/sample). Even difficult samples such as 0.1N NaOH mix rapidly to yield a clear,

non-viscous emulsion.

� Non hazardous and Biodegradable

� Low Viscosity

� Non-Gelling

� Ultra-High Sample Hold (1:1

Cocktail to Sample) Low Toxicity

� High Counting Efficiency

Ecoscint FlowTM

9


Monoflow 2 Flow scintillation cocktail for routine, low-salt, aqueous effluents. LS-2824 liter bottle £57.64


Monoflow 3Flow scintillation cocktail providing high sample holding capacity for routine, lowsalt effluents.



Monoflow 4Flow scintillation cocktail for high salt aqueous amples. Can accomodate 2M saltgradients.



Ecoscint FlowEcoscint Flow is a low viscosity, ultra-high sample hold, biodegradable scintillationcocktail ideal for HPLC flow counting.

LS-2881x4 liter bottle £82.45


Bioscint Biodegradable Tissue Solubilizer/ Scintillator Combination Non hazardous WasteClear Emulsions for Excellent Counting

LS-309 1x4 liter bottle £93.28

Biosol LS-310 400 ml £64.34

Solusol A superior solubilizing agent which may be used with a wide variety of samples. LS-311 450 ml £94.83

Soluscint XR Companion Cocktail for Solusol Ideal for High Salt and Alkaline Samples LS-314 4 liter £57.64

AutofluorWater soluble scintillation phosphor for autoradiographic image intensification.Safer and more effective than PPO-DMSO.

LS-315 1 liter £92.64

Bottle-top Dispenser LS-900 1 Dispenser P.O.A.

Extendable Delivery Jet LS-904 1 Jet P.O.A.

NucleanConcentrated, effective, economical solution for removing radioactivity fromlaboratory glassware, equipment, and surfaces.

NC-2001 quart

£8.10

1 gallon £29.21

Nuc-WipesUnique pads for environmental wipe tests which dissolve in scintillation fluid,ensuring accurate, reproducible results.

NW-300 1 box (100/box) £13.52

Scintillation Vials6 mL

(HDPE) scintillation vials manufactured as a one-piece moulding with no seams,proving excellent UV light transmission for high counting efficiency.

SVC-06 1 case (2500) £81.58

Scintillation Vials8 ml

SVC-08 1 case (2000)

Scintillation Vials20 mL

SVC-20 1 case (1000) £62.15

NATIONAL DIAGNOSTICSElectro-Optical Cleaning Agents


10

To reduce the environmental impact and occupational health and safety hazards associated with toxic organic

clearants in the laboratory and in industry, National Diagnostics developed its original, award-winning OptiClearTM

cleaning solvent. As a result of ever growing demand for safe products for cleaning optical and electroniccomponents, National Diagnostics expanded the OptiClear product line to include an array of lower hazardsolvents.


OptiClearAward winning, nontoxic, nonflammable, biodegradable solvent,intended for the removal of wax, pitch, flux, grease, and resin fromoptical and electronic components of glass, ceramic, or metal.

OE-101

16 oz. spray bottle £24.96

1 gallon £40.52

5 gallon drum £195.94

OptiClear RWater based solvent intended as an acetone replacement forcleaning optical and electronic components.

OE-102

2 oz. squeeze bottle £8.49


1 gallon £38.20

OptiClear EOur fastest evaporating OptiClear formulation, for the removal of waxand pitch.

OE-104 16 oz. spray bottle £25.47

OptiClear E OE-104 1 gallon £42.46

OptiClear SOptiClear S has a higher flash point and evaporates more slowly thanOptiClear, for the removal of wax and pitch.

OE-105


1 gallon £42.46


OptiClear WOptiClear W is a nontoxic, nonflammable, biodegradable solventintended to remove grease and oil from optical and electroniccomponents. OptiClear W is miscible in all proportions in water.

OE-106


1 gallon £53.02


Opticlear S2 Our highest flash point solvent, for the removal of wax and pitch. OE-107 16 oz. spray bottle £29.72

1 gallon £54.30

Opticlear Sample Kit I 1 liter each of OptiClear, OptiClear R, OptiClear S, OptiClear W. OE-108 1 liter each £22.91

Opticlear Sample Kit II1 liter each of OptiClear, OptiClear E, OptiClear R, OptiClear S,OptiClear W.

OE-109 1 liter each £31.70

AGTC Bioproducts


Microbiology Media11

AGTC Bioproducts are pleased to be able to bring you some ofthe best quality products for bacterial growth, identification andmanipulation

� Comprehens ive Microb io logy Protocol Cata logue� Large se lect ion o f Pre -Formulated Growth Media� Extens ive range o f Peptones and Supp lements

We are one of the world leaders in the distributionof high quality culture media, we are ablecurrently, to offer more than 400 differentproducts, among which you will find chromogenicmedia, ISO-formulated media and custom-mademedia for many different industrial applications.From hygiene control, through food and beveragecomtamination prevention, to microbiologialexamination of cosmetic and pharmaceuticalproducts, We supply a wide variety of differentmedia for each field so that customer are able tofind the most suitable product for their needs. Wecomply with ISO 9001 , and our products complywith various international standards such as ISO,the European Pharmacopoeia, FDA, APHA, USPand AOAC standards. Strict quality controlprocedures are adopted prior to, during and after

the manufacturing process to ensure qualityproducts and batch-to-batch consistency. We alsoexert tight control over selection and treatmentof all raw materials and components (peptones,carbohydrates, minerals, chemicals, agar andother additives) used in the manufacturingprocess. Physical-chemical characteristics aretested, and media also undergo additionalmicrobiological tests that guarantee growth,differentiation, biochemical performance,recovery of small binocular, selectivity, etc. Aquality control certificate is supplied with eachproduct. Our reputation and success throughoutthe world are the result of a combination ofuncompromising quality systems, technicalexpertise and innovative thinking.

Code Product Price (500 g) Code Product Price (500 g)

AG-1100 BAIRD PARKER AG-AR BASE £65.66 AG-1062 MANNITOL SALT AG-AR £25.09

AG-1108 BLOOD AG-AR BASE £43.05 AG-1058 MUELLER HINTON AG-AR £44.33

AG-1048 BRAIN HEART INFUSION AG-AR (BHI AG-AR) £69.64 AG-1240 RAPPAPORT SOY BROTH £39.18

AG-1010 BRILLIANT GREEN AG-AR £90.14 AG-1081 ROSE BENGAL AG-AR £44.41

AG-1402 BUFFERED PEPTONE WATER £31.29 AG-1220 SELENITE CYSTINE BROTH £50.57

AG-1104 COLUMBIA AG-AR BASE £36.92 AG-1056 STANDARD METHODS AG-AR (P.C.A) £37.57

AG-1027 CONFIRMATORY K.A.A. AG-AR £67.41 AG-1070 STREPTOCOCCUS SELECTIVE AG-AR £40.76

AG-1028 DNAse TEST AG-AR £67.08 AG-1068 T.S.A. £35.19

AG-1508 FLUID THYOGLICOLLATE MEDIUM (FTM) £36.42 AG-1224 T.S.B. £29.07

AG-1203 GLUCOSE BROTH £39.07 AG-1092 V.R.B.G. £29.07

AG-1052 MAcCONKEY AG-AR £35.24 AG-1093 V.R.B.L. £36.87

CLINICAL AND INDUSTRIAL MICROBIOLOGY

PEPTONES + AGAR

Code Product Price (500 grams)

AG-1616 BACTERIOLOGICAL PEPTONE £38.02

AG-1800 EUROPEAN BACTERIOLOGICAL AGAR £51.48

AG-1600 MEAT PEPTONE £46.49

AG-1612 TRYPTONE £48.76

AGTC Bioproducts


Microbiology Media 12

Meat and Fish IndustriesThe increase in international trade in recent years,has made necessary the use of faster, moreefficient and more specific microbiologicaldiagnostic culture media for each individualindustry and each pathogen.

Meat and fish products are highly perishable. Thetype of microorganisms present is variable andvery much depends on the product. Although themuscle itself is virtually sterile, meat-basedprocessed foods are very susceptible tocontamination providing favorable conditions forgrowth of microorganisms that can causepoisoning and food-borne illness.

We can offer a wide range of specific culturemedia for the meat and fish industry.

Most important for this industry is the pathogenListeria monocytogenes. This gram-positivebacteria has been the cause of numerousoutbreaks of foodborne diseases in the lastdecade. Listeriosis is usually found in pregnantwomen, their fetuses and in people with immuneproblems; symptoms are commonly abortion,neonatal death, septicemia and meningitis, witha mortality rate exceeding 20%.

Listeria is usually found in most food, but thehighest rates are in meat, poultry and seafood. Itsurvives periods of cold storage and it grows attemperatures as low as 0°C. It also survives at highsalt concentrations for long periods of time and isrelatively resistant to dehydration.

Some specific media for Listeria:

Some media for the identification of other pathogens important to meat industry safety:

Code Description Size Price

AG-1182 LISTERIA FRASER BROTH BASE (ISO 11290-1) 500 grams £58.15

AG-1133 LISTERIA AGAR BASE OXFORD: Detection . 500 grams £79.30

AG-1141 LISTERIA AGAR BASE PALCAM: Selective differential medium for detection . 500 grams £70.48

AG-1345 LISTERIA CHROMOGENIC AGAR BASE : Selective medium for detection and enumeration. 500 grams £110.13

AG-1120 LISTERIA ENRICHMENT BROTH BASE FRASER (ISO 11290-1): Detection and isolation. 500 grams £52.86

AG-1398 TRYPTONE SOY YEAST EXTRACT AGAR (TSYEA) (ISO 11290-1): Growth of Listeria spp. 500 grams £37.22

AG-1339 TRYPTONE SOY YEAST EXTRACT BROTH (TSYEB) (ISO 11290-1): Confirmation of Listeria monocytogenes . 500 grams £29.85

Code Description Size PriceAG-1400 BRAIN HEART INFUSION BROTH (B.H.I. BROTH): Confirmation of positive-coagulase Staphylococcus . 500 grams £47.41

AG-1219 BRILLIANT GREEN SELENITE BROTH II : Selective enrichment of Salmonella species . 500 grams £73.67

AG-1044 LYSINE IRON AGAR : Studies of decarboxylation of lysine for rapid differentiation of Salmonella arizonae. 500 grams £56.52

AG-1356 PSEUDOMONAS AGAR BASE (ISO 13720) : Isolation, detection and enumeration of Pseudomonas . 500 grams £44.90

AG-1096 ROGOSA SL AGAR: Selective media for cultivation of Lactobacilli. 500 grams £50.13

AG-1234 ROGOSA SL BROTHS: Elective media for cultivation of Lactobacilli. 500 grams £38.66

AG-1064 SALMONELLA SHIGELLA AGAR (S.S.): Selective medium for isolation of Salmonella and Shigella . 500 grams £47.06

AG-1082 S. P.S AGAR: Isolation of Clostridium perfringens . 500 grams £61.74

AG-1029 TSC AGAR BASE (ISO 7937): Isolation of Clostridium perfringens . 500 grams £46.26

AG-1075 TSN AGAR: Isolation of Clostridium perfringens . 500 grams £51.03

AGTC Bioproducts



Water & BeveragesNatural water supplies such as rivers and lakes andstreams contain sufficient nutrients to supportgrowth of various organisms. Microorganisms enterthe water supply in many different ways. In congestedcenters water supplies are polluted by domestic andindustrial waste. As a potential carrier of pathogenicmicroorganisms, water can endanger health and life.Drinking water is obtained from different sources.Various impurities may occur in these waters thathave to be removed before the water is supplied tothe public. To render the water safe for drinking ithas to be treated property.Proper sampling is essential to provide representativealiquots to the laboratory conducting the tests.Microorganisms are living organisms. In addition,when found in water, they are not a perfect solution,but a suspension with a degree of variability that isinherent to it. The presence and level of fecalcontamination is an important factor in assessing thequality of water bodies and the risk of infection posedto human health. The analysis of water samples to

detect Escherichia coli, which normally inhabits theintestinal tract of humans and other warm-bloodedanimals, provides an indication of this type ofcontamination.Other indicators of fecal contamination areEnterococci. Such microorganisms are particularlyimportant when the contamination occurred sometime ago and the less resistant coliform bacteria,including Escherichia coli, may have died by the timethe analysis is performed. Pseudomonas aeruginosais an opportunistic pathogen for humans that iscapable of growing in water with very low nutrientconcentrations. It is desirable that natural mineralwaters, spring waters, bottled waters and waters forhuman consumption are free of Pseudomonasaeruginosa. Sometimes, for reasons of public healththe presence of Pseudomonas in other types of watermay be investigated, including water in swimmingpools.For the identification of all these key pathogens in theanalysis of water we suggest the following media:Water in general:

Code Description Size PriceAG-1071 R2A AGAR (Eur. Pharma.) Recommended by the European Pharmacopoeia for total aerobe count in treated water. 500 grams £49.46

Code Description Size PriceAG-1005 BILE ESCULIN AZIDE AGAR (ISO 7899-2): Provides rapid growth and efficient recovery of Enterococci 500 grams £91.10

AG-1037MACCONKEY AGAR WITHOUT CRYSTAL VIOLET: Investigation of enteric microorganisms, especially Enterococci, fromwater.

500 grams £35.19

AG-1109 SLANETZ-BARTLEY MEDIUM (ISO 7899-2): Enumeration of Enterococcus faecalis in water systems. 500 grams £60.99

AG-1412 M-EI AGAR BASE CHROMOGENIC: detection and enumeration of Enterococci in water membrane-filtration technique . 500 grams £155.95

Code Description Size PriceAG-1127 FECAL COLIFORMS AGAR BASE (m-FC); Membrane-filter medium for the detection and enumeration of fecal Coliforms . 500 grams £50.77

AG-1357 PEPTONE WATER WITH LACTOSE (ISO 9308-1): confirmation of Coliforms in water . 500 grams £37.04

AG-1138TRYPTONE SOY AGAR (ISO 9308-1): Detection and enumeration of E.coli and other Coliform bacteria by membrane-filtration .

500 grams £35.40

AG-A51076

TTC CHAPMAN AGAR (SO 9308-1: Recommended for the enumeration of Coliforms in drinking water by membrane -filtration technique .

500 grams £34.67


AG-1532KING B MEDIUM (PSEUDOMONAS F AGAR) (USP): Identification and enumeration of Pseudomonas aeruginosa bymembrane-filtration .

500 grams £47.28

AG-1060 NUTRIENT AGAR: Identification and enumeration of Pseudomonas aeruginosa by membrane-filtration 500 grams £40.70

AG-1153PSEUDOMONAS CN AGAR BASE (UNE-EN 12780): Identification and enumeration of Pseudomonas aeruginosa bymembrane-filtration .

500 grams £73.07


AG-1132CLOSTRIDIUM PERFRINGES AGAR BASE (m-CP) : Used in the two-step membrane-filtration method for detection ofClostridium perfringens in environmental water and all other types of water .

500 grams £71.22

AG-1311LEGIONELLA CYE AGAR BASE : Optimum for the cultivation of Legionella with shorter incubation periods fromenvironmental and clinical samples.

500 grams £60.00

AG-1049YEAST EXTRACT AGAR (ISO 6222):these are nutrient medium for enumeration of a wide spectrum of bacteria, yeast andmolds .

500 grams £38.24

AGTC Bioproducts




AG-1005 BILE ESCULIN AZIDE AGAR (ISO 7899-2):

Isolation and identification of Group D Streptococci. Foods of animal orvegetable origin obtained by fermentation may have a very high contentof group D streptococci, which are present as natural flora. TheseStreptococci can be used as indicators of highly resistant entericpathogens. They are also useful in the analysis of thermally processedfoods and in determining the effectiveness of disinfection and cleaningsystems.

500 grams £91.10

AG-1247BRYANT & BURKEY BASE BROTH(modified with resarzurine):

Detection of lactate fermenting clostridial species, in milk and dairyproducts, particularly Clostridium tyrobutyricum. This bacterium isresponsible for the "late spoilage" in certain types of cheese and it givesthem an unpleasant taste and odor, which comes to crack and even popcheese.

500 grams £58.65

AG-1301 CHLORAMPHENICOL AGAR (ISO 7954):Selective medium for isolation and enumeration of molds in milk anddairy products. Recommended by ISO.

500 grams £39.55

AG-1020 DESOXYCHOLATE AGAR : Isolation and enumeration of Coliforms in dairy products. 500 grams £38.77

AG-1254 E.S.T.Y. BROTH: Selective media for cultivation of Streptococcus thermophilus in yogurt.Presence of this microorganism is the main difference betweentraditional yogurts and the yogurts "pasterized after fermentation" (thatdon't need cold temperature), which don't contain it.

500 grams £44.76

AG-1555 E.S.T.Y. Medium: s 500 grams £48.87

AG-1094 GLUCOSE CHLORAMPHENICOL AGARSelective media for isolation and identification of yeasts and molds indairy products.

500 grams £42.48

AG-1258GLUCOSE CHLORAMPHENICOL BROTH

500grams £26.22

AG-1027 K.A.A. CONFIRMATORY AGAR Isolation and confirmation of Lancefield Group D Streptococci in foods.(see Bile Esculin Agar).

500grams £67.41

AG-1209 K.A.A. PRESUMPTIVE BROTH 500 grams £87.85

AG-1445LAURYL SULPHATE TRYPTHOSE BROTHMODIFIED (m LST) (ISO 22964)

Selective enrichment medium for Enterobacter sakazakii (seeEnterobacter sakazakii Isolation Chromogenic Agar).

500 grams £28.86

AG-1318 M 17 AGAR

cultivation and enumeration of lactic Streptococci in milk and dairyproducts. Recommended for isolation of Streptococcus thermophilus inyogurts. Also for cultivation and maintenance of cheese and yogurtfermentations.

500 grams£61.05

AG-1238ROTHE BROTH (Glucose Broth withAzide)

For the quantitative determination of fecal Streptococci.500 grams £39.46

AG-1033STANDARD METHODS AGAR WITHPOWDERED MILK (ISO 4833)

For bacterial plate counts of microorganisms from milk and dairyderivatives (APHA* Formula).

500 grams £44.71

Dairy productsChemical analysis carried out on dairy productsare of considerable importance in the qualitycontrol of finished products, for example todetermine the freshness period of a product, orits expiration date.Food safety is essential in milk and dairy productsindustry. The healthy image that these productshave, particularly the perception of their safetyand quality, is always under close scrutiny due tooutbreaks of human illnesses and product recallsimplicating milk, cheese, ice creams, etc.

Therefore, thorough testing with the correctculture media is essential to ensure safety forconsumption.

For the identification of these pathogens we haveselected several typically specific culture mediafor this industry:

AGTC Bioproducts




AG-1022 POTATO DEXTROSE AGAR (Eur. Pharma.)

identification, cultivation and enumeration of yeasts and molds. Thecontent of potato (a source of starch) and the low pH favor the growthof those yeasts and molds

500 grams £34.99

AG-1160ROSE BENGAL AGAR + DICHLORAN +CLORAMPHENICOL (DRBC AGAR)

selective medium for the enumeration of yeasts and molds in food.Bengal rose is a selective agent that inhibits the growth of bacteria andlimits the size and height of faster-growing molds, allowing for thedevelopment of other slower-growing fungi.

500 grams £59.31

AG-1527O.G.A. MEDIUM (Oxytetracycline GlucoseAgar)

enumeration and selection of yeasts and molds in food samples.500 grams £29.46

AG-1124 BACILLUS CEREUS SELECTIVE AGAR BASEenumeration and isolation of Bacillus cereus in food, according toMOSSEL.

500 grams £38.02

Bakery industryWithin the broad spectrum of the food industry,one of the most commonly consumed dailyarticle is bread. Today, both industrial as well astraditional bakeries use additives to control thegrowth of bacteria and molds in their products,but to meet legal requirements and adhere tohygiene standards during preparation they mustalso test for microorganisms. The prior controlof raw materials and their control during thepreparation process is vital to obtaining a finalproduct free of microorganisms.

After baking, the environment makes an idealculture medium for the development airbornespores, so it is vitally important to treat thistype of product in its final format. This kind of

contamination is more likely to happen in springand early summer and in places of high dust orflour concentration. Bread fungi are aerobic andvegetative microorganisms, meaning they needoxygen to reproduce themselves, so it iscommon for fungi to proliferate first in the crust,which is the area most exposed to air inside thepackage.

For the identification of all these pathogens ofimportance in the safety of the bakery industry,there are several important culture media:

ISO 6579:2002 specifies a horizontal method for the detection of Salmonella, including Salmonella Typhi andSalmonella Paratyphi. It is applicable to products intended for human consumption and the feeding of animals, andto environmental samples in the area of food production and food handling. Everybody involved in these areasshould conform to this standard when testing for microbial contamination.

Why you should use our media for ISO 6579:2002

PRE-ENRICHMENT

SELECTIVE

ENRICHMENT

PLATE

CONFIRMATION

Buffered peptone waterCat No. 1402

RVS BrothCat No. 1174

MKTTn BrothCat No. 1173

UreaIndolTSI Agar Cat 1172.β GalactosidaseLisine Decarboxilase Broth Cat No 1176Voges-Proskauer

XLD Agar Cat.1274 &Salmonella Chromogenic

Agar Cat.1122

XLD Agar Cat.1274 &Salmonella Chromogenic

Agar Cat.1122

Biochemical Tests

Salmonella

A fully detailed poster schematic for this standard is available free for download from our website.

AGTC Bioproducts



Processed FoodMicroorganisms can cause a variety of effects in foodproducts including spoilage which primarily affectsproduct quality, and food poisoning, which is generallycaused by pathogens, .

Bacteria can enter food products through differentroutes. In food processing, foodborne microbes can beintroduced from infected humans who handle the food,or by cross contamination from some other raw materialand/or the in-plant environment. For example, bacteriaand viruses can be introduced by the unwashed handsof food handlers who are themselves infected.

Product that is fully cooked can become crosscontaminated if it comes into contact with rawmaterial containing pathogens or with food contact

surfaces that are contaminated.

In the kitchen, microbes can be transferred from onefood to another by using the same knife, cutting boardor other utensil to prepare both without washing thesurface or utensil between.

The way that food is handled after it is contaminatedcan also make a difference in whether or not, anoutbreak occurs.

Culture media for Beer industry

Beer is not a very appropriate medium for thedevelopment of bacteria due to its characteristics, suchas the low quantity of available nutrients, the presenceof alcohol, carbon dioxide and sulphur dioxide, as wellas low conservation temperatures. Beer filtration andpasteurization phases also contribute to thestabilization of the product against microorganisms.The number of genera and species which usuallycontaminate it is limited. As is the case with wild yeasts,the contaminating bacteria cause turbidity andgenerate anomalous smells and bouquets. Lactic acidbacteria are the only Gram-positive ones that causeserious problems within beer-producing industries. Thefrequently found ones only belong to two genera:Lactobacillus and Pediococcus. Wild yeasts are alsofrequent contaminants in beer production.

Culture media specific for beer:

In order to guarantee a process free ofmicroorganisms, equipment and facilities should bekept in a perfect state of hygiene since contaminatingelements almost always locate themselves in tanks,pipes, hoses, etc. It is also necessary to subject allequipment and raw materials including water, malt,triturates, hops, etc., to these controls.


AG-1438 NBB AGAR, MODIFIED Selective medium used for the detection ofContaminating/spoilage micro-organisms in brewery

500 grams £34.99

AG-1440 NBB BROTH, MODIFIED 500 grams £59.31

AG-1061 RAKA - RAY AGAR BASESelective medium to isolate lactic-acid bacteriain beer and beer fermentation processes

500 grams £44.76

AG-1086 W.L. NUTRIENT AGARFor the determination of microbial floraCat : 1086 in beer fermentation processes and manufacturing

500 grams £37.68

AGTC Bioproducts



We also supply bacteriological media for the following industrial sectors:

Waste water.

Cosmetics.

Pharmaceutical products.

Full details of all our 500 or more different products for bacteriological detection can be foundon our web site, via our online catalogue at www.agtcbioproducts.com

AGTC Bioproducts


Synthesis Reagents 18

Cat. No Name 5g 25g 100gAGM01 Fmoc-L-Ala-OH £6 £15 £43

AGM02 Fmoc-L-Arg(Pbf)-OH £40 £140 £423

AGM03 Fmoc-L-Asn(Trt)-OH £25 £59 £176

AGM04 Fmoc-L-Asp(tBu)-OH £25 £56 £166

AGM05 Fmoc-L-Cys(Trt)-OH £25 £59 £176

AGM06 Fmoc-L-Gln(Trt)-OH £25 £59 £176

AGM07 Fmoc-L-Glu(tBu)-OH £25 £56 £166

AGM08 Fmoc-Gly-OH £6 £15 £43

AGM09 Fmoc-L-His(Trt)-OH £22 £43 £130

AGM10 Fmoc-L-Ile-OH £6 £15 £41

AGM11 Fmoc-L-Leu-OH £6 £15 £41

AGM12 Fmoc-L-Lys(Boc)-OH £20 £43 £130

AGM13 Fmoc-L-Met-OH £6 £15 £41

AGM14 Fmoc-L-Phe-OH £6 £15 £41

AGM15 Fmoc-L-Pro-OH £6 £15 £41

AGM16 Fmoc-L-Ser(tBu)-OH £25 £46 £140

AGM17 Fmoc-L-Thr(tBu)-OH £25 £46 £140

AGM18 Fmoc-L-Trp(Boc)-OH £31 £94 £283

AGM19 Fmoc-L-Tyr(tBu)-OH £25 £43 £130

AGM20 Fmoc-L-Val-OH £6 £15 £41

Fmoc Amino Acids(Fmoc-D-Amino Acids & Boc (L & D) Amino Acids also available)

Cat.No Product Size PriceAGM16.3 Fmoc-L-Ser(PO(OBzl)OH)-OH 5g £391

AGM16.3 Fmoc-L-Ser(PO(OBzl)OH)-OH 10g £743

AGM17.2 Fmoc-L-Thr(PO(OBzl)OH)-OH 5g £430

AGM17.2 Fmoc-L-Thr(PO(OBzl)OH)-OH 10g £818

AGM19.4 Fmoc-L-Tyr(PO(OBzl)OH)-OH 5g £484

AGM19.4 Fmoc-L-Tyr(PO(OBzl)OH)-OH 10g £920

Phospho Fmoc Amino Acids

Cat. No Product 100g 500g 1 KgAG330645 HCTU £110 £331 £579

AG94790 HBTU £91 £171 £228

AG123333 HOBt . H2O £32 £107 £214

AGX1060 TBTU £60/50g £72/100g

AG148893 HATU £317

AG39968 HOAt £165/25g

AGBC7012 DIPEA £55.65/Litre

Reagents & Linkers(much more inside catalogue)

Peptide Synthesis reagents and solvents These peptide synthesis reagents are manufactured in wellequipped modern production facilities. All products are fully QC'd by thorough assay methodologies to check forchemical and chiral purity. All Solvents are fully QC’d for low levels of water, free amine and residue/particulates.

Name Cat. No size PriceAcetonitrile 190 UV (HPLC)CAS Number: 75-05-8EC Number: 200-835-2

AGMHP1008 -2.5lt 2.5 Ltr P.O.A.

DichloromethaneEC Number: 200-838-9CAS Number: 75-09-2assay ≥99.8% (GC)

AGMBC7002 -2.5lt

2.5 Ltr £12.60

4 Ltr £20.16

25 Ltr £50.32

200 Ltr £360.00

Di-isopropylethylamineCAS Number: 108-18-9assay ≥99.5%

AGBC7012 -1lt 1 Ltr £55.65

DimethylformamideCAS Number: 68-12-2assay ≥98.0% (GC)

AGMHP1044 -2.5lt

2.5 Ltr £17.00

4 Ltr £26.25

25 Ltr £100.00

200 Ltr £339.00

DMF80%/Piperidine20% Premix AGMPIPDMF -4lt4 Ltr £56.70

25 Ltr P.O.A.

N-MethylpyrollidoneEC Number: 212-828-1assay ≥99.5% (GC)

AGMBC7006 -2.5lt

2.5 Ltr £27.30

4 Ltr £44.10

25 Ltr £123.00

200 Ltr £811.00

PIPERIDINECAS Number: 110-89-4assay ≥99.5%

AGMPIP450 -450ml 450ml £22.05

Solvents For Synthesis

AGTC Bioproducts


Transfection 19

� High-Efficiency-Transfection Reagent Low Toxicity Simple Procedure� Low Cost

HiTranTM is a Polylipid-based reagent designedand formulated with proprietary technology fortransfecting DNA or siRNA into a variety ofeukaryotic cell lines, including HEK293, CHO, COS,NIH3T3, MCF-7, PC12, RKO, C2C12 myoblasts,mouse embryonic stem cells (ES) and fibroblasts(MEF), mesenchymal stem cells, NCCIT, S2

(Schneider) Drosophila cells as well as primarycultures of human carcinoma, rat adrenalchromaffin cells, mouse and rat cortical neurons,

hepatocytes, etc. HiTranTM also offers extremelyhigh transfection efficiencies and minimalcytotoxicity for suspension cultures including HEK293, mouse lymphocytes, and insect cells (sf9,sf21, High-Five). The reagent works very well incultures with serum concentration range from 0to 20% FBS.GFP expression in different cell lines:

C O C2C1H E KP C 1

Transfection Reagents Description Size Price

AGTC-HT-1211-01

High Efficiency Transfection Reagent

0.5mL £93.75

AGTC-HT-1211-02 1.0mL £175.00

AGTC-HT-1211-03 1.5mL £242.50

AGTC-HT-1211-04 6.0mL £867.50

AGTC-HT-1212-01

Transfection Reagent for Transient Protein Expression

0.5mL £100.00

AGTC-HT-1212-02 1.0mL £187.50

AGTC-HT-1212-03 1.5mL £262.50

AGTC-HT-1212-04 6.0mL £937.50

HiTran 60µl/30µg vectors/per 15 cm plate

DAPI GFP combined

R2000 60µl/30µg vectors/per 15 cm plate

DAPI GFP combined

Transfection of HEK293 cells with triple lentivirus vectors using HiTran and R2000:

Plasmid Purification


20

� Superior Purity� Ultra High Yield� Time Saving� Eco-Friendly and Low Cost

"A Breakthrough Plasmid DNA PurificationSystem"

Superior Purity: This patented Plasmid Purificationsystem uses no chaptropic salts in the buffer, and theresulting purified DNA is free of guanidine salts,caesium chloride, ethidium bromide and ionexchange resin residues, which enable the highestperformance of downstream applications includinggene therapy and genetic vaccination.

Ultra High Yield: When comparing our' PlasmidPurification System to other purification methods,

our methodology produces higher DNA yield whilerequiring fewer bacterial cultures.

Time Saving: Using our unique Megaprep Kit, 10 mgPlasmid DNA can be purified from 1,000 mL culturewithin 1 hour!

Environment benefit: The Quickgene System is theonly method available that does not utilize GuanidineSalt, a hazardous protein denaturant, in the silicabased purificationsystem, which willensure the safetyand health ofscientists and theenvironment!

KIt Description

PlasmidMiniprep

Easy and reliable methods to purify plasmid DNA from 1 mL to 12 mL bacterial cultures in less than 20 minutes.The DNA is ready for downstream applications such as restriction digestions, PCR, and automated sequencing.� Kit I: 10‐20 μg of high copy number plasmid DNA can be isolated from 1‐2 mL culture. Kit II: 30‐80 μg from

5-12 mL culture.

PlasmidMidiprep

This kit provides an easy and reliable method to purify plasmid DNA from 50-100 mL bacterial cultures in lessthan 60 minutes. The DNA is ready for downstream applications such as transfection, RFLP, DNA amplification,and automated sequencing.� Kit I: 100‐200 μg of high copy number plasmid DNA can be isolated from 50 mL culture. Kit II: 200‐400 μg

from 100 mL culture.� Choice of 2 types of columns for centrifugation or vacuum manifold. Endofree Midiprep: Endotoxin < 0.2

EU / μg DNA.

PlasmidMaxiprep

This kit provides an easy and reliable method to purify plasmid DNA from 150 to 250 mL bacterial cultures inless than 60 minutes. The DNA is ready for downstream applications such as transfection, RFLP, DNAamplification, and automated sequencing.� 1000‐1500 μg of high copy number plasmid DNA can be isolated from 200 mL culture.per column� Choice of 2 types of columns for centrifugation or vacuum manifold.� Endofree Maxiprep: Endotoxin < 0.2 EU / μg DNA.

PlasmidMegaprep

Based on our proprietary technology and unique design, milligrams of high quality DNA can be purified within anhour. The purified DNA is ready for downstream applications such as transfection, RFLP, DNA amplification, andautomated sequencing.� 2-layer filter unit.� No centrifugation in the entire procedure.� Megaprep 3/6: 3 - 6 mg of high copy plasmid DNA can be purified from 1000 mL culture in 60 minutes.� Megaprep12: up to 12 mg of high copy plasmid DNA can purify from 2000 mL culture in 60 minutes.� Endofree Megaprep: Endotoxin < 0.2 EU / μg DNA.� The endofree Mega kits are especially suitable for DNA vaccination and gene therapy applications.

96-wellPlasmidMiniprep

This high-through-put system is designed for the parallel preparation of 96 plasmid minipreps within 1 hour. Thepurified DNA is ready for downstream applications such as DNA amplification, automated sequence, RFLP,transfection, and viral packaging.� Fast and reliable.� 96 DNA preps can be done in less than 40 minutes.� One-step filter-binding, which omits lysate transferring from plate to plate.� 8‐12 μg DNA per well for high copy plasmid from 1.5 mL culture.� Can be adapted to many automated platforms


Plasmid Purification21

Code Description No : Price

AG-PD1211 Plasmid Miniprep Kit I : 10‐20 μg of high copy number plasmid DNA can be isolated from 1‐2 mL culture50 £28.1

250 £137.5

AG-A26PD1213Plasmid Miniprep Kit II:Kit II: 30‐80 μg of high copy number plasmid DNA can be isolated from from 5‐12mL culture.

50 £37.5

250 £156.3

AG-PD1411 Plasmid Midi Kit: 100‐200 μg of high copy number plasmid DNA can be isolated from 50 mL culture10 £32.5

25 £78.1

AG-PD1412 Plasmid Midi Kit II 200‐400 μg of high copy number plasmid DNA can be isolated from 100 mL culture.10 £49.4

25 £109.4

AG-PD1413 Plasmid ezFilter Midi Kit I10 £49.4

25 £109.4

AG-PD1414 Plasmid ezFilter Midi Kit II10 £55.6

25 £121.9

AG-PD1511 Plasmid Maxi Kit10 £75.0

25 £156.3

AG-PD1512 Plasmid ezFilter Maxi Kit10 £81.3

25 £175.0

AG-PD1611 Plasmid ezFilter Mega3 Kit2 £50.0

10 £237.5


10 £287.5


10 £425.0

AG-PD1811 96-well Plasmid Mini Kit4x96 £250.0

20x96 £1,250.0

AG-PD1812 96-well Plasmid ezFilter Mini Kit4x96 £312.5

20x96 £1,437.5

AG-PD1212EndoFree Plasmid Mini Kit I

50 £36.3

250 £156.3

AG-PD1214 EndoFree Plasmid Mini Kit II50 £42.5

250 £181.3

AG-PD1415 EndoFree Plasmid Midi Kit10 £49.4

25 £109.4

AG-PD1416 EndoFree Plasmid ezFilter Midi Kit10 £56.3

25 £125.0

AG-PD1514 EndoFree Plasmid Maxi Kit10 £87.5

25 £175.0

AG-PD1515 EndoFree Plasmid ezFilter Maxi Kit10 £93.8

25 £187.5

AG-PD1615 EndoFree Plasmid ezFilter Mega3 Kit2 £55.0

10 £250.0


10 £312.5


10 £450.0

AG-D1711 HP Plasmid Mini Kit50 £37.5

250 £162.5

AG-D1712 HP Plasmid Midi Kit10 £43.1

25 £100.0

AG-D1713 HP Plasmid Maxi Kit10 £100.0

25 £237.5

AG-D1714 HP Plasmid ezFilter Mega3 Kit2 £56.3

10 £250.0

AGTC BioproductsDithiothreitol 22

Dithiothreitol (DTT) Cleland's Reagent

DTT is an unusually strong reducing agent, used extensively in organic reactions. Our DTT is producedto the highest standards possible (see attached technical specification) and with additionally extremelylow levels of Bromine and Lead. The synthesis we use produces a particularly odour reduced productmaking it highly suitable for commercial kit manufacture.

Some applications are:

As a reducing or "deprotecting" agent for thiolated DNA.

Reduction of disulfide bonds in proteins.

Prevent intramolecular and intermolecular disulfide bonds from forming between cysteineresidues of proteins.

DTT can also be used as an oxidizing agent.

For larger sizes 100g-to kilo’s call us today to receive a free evaluation sample and a competitivequote:

Cat No Name PriceEC-601-25gm Dithiothreitol DTT, >98% purity, low odour £49EC-601-50g Dithiothreitol DTT, >98% purity, low odour £89

A common use of DTT is as a reducing or"deprotecting" agent for thiolated DNA. Theterminal sulphur atoms of thiolated DNA have atendency to form dimers in solution, especially inthe presence of oxygen. Dimerization greatlylowers the efficiency of subsequent couplingreactions such as DNA immobilization on gold inbiosensors. Typically DTT is mixed with a DNAsolution and allowed to react, and then is removedby filtration (for the solid catalyst) or bychromatography (for the liquid form). The DTTremoval procedure is often called "desalting."DTT is frequently used to reduce the disulfidebonds of proteins and, more generally, to preventintramolecular and intermolecular disulfide bondsfrom forming between cysteine residues ofproteins. However, even DTT cannot reduceburied (solvent-inaccessible) disulfide bonds, so

reduction of disulfide bonds is sometimes carriedout under denaturing conditions (e.g., at hightemperatures, or in the presence of a strongdenaturant such as 6 M guanidiniumhydrochloride, 8 M urea, or 1% sodiumdodecylsulfate). Conversely, the solvent exposureof different disulfide bonds can be assayed by theirrate of reduction in the presence of DTT.DTT can also be used as an oxidizing agent. Itsprincipal advantage is that effectively no mixed-disulfide species are populated, in contrast toother agents such as glutathione. In very rarecases, a DTT adduct may be formed, i.e., the twosulfur atoms of DTT may form disulfide bonds todifferent sulfur atoms; in such cases, DTT cannotcyclize since it has no remaining free thiols.

CAS number 3483-12-3

Properties

Molecular formula C4H10O2S2

Molar mass 154.25 g mol

Appearance White solid

Melting point 42-43 °C

Boiling point 125-130 °C at 2 mmHg

Solubility in water Soluble

other names

(2S,3S)-1,4-dimercaptobutane-2,3-diol

Threo-1,4-dimercaptobutane-2,3-diol

DL-Threo-1,4-dimercapto-2,3-butanediol

1,4-Dithio-DL-threitol

±-Threo-1,4-dimercapto-2,3-butanediol

Cleland's reagent

Reductacryl

23Terms & Conditions (2010)In these Conditions the 'Seller' means AGTC Bio products Limited, 'the Buyer' means the person, firm, institute or Company purchasing the goods. 'theGoods' means the goods or materials which shall be the subject of the contract between the Seller and the Buyer 'the Price' means the price specifiedin the Sellers Quotation, Acknowledgment or otherwise communicated to the Buyer and agreed.SPECIFICATIONSSpecifications and detail in catalogues and leaflets are subject to amendment without notification. The right is reserved by the Seller to supply goods tothe specification and/or design current at the date of dispatch.CATALOGUES, DRAWINGS, SKETCHESAll statements (whether written or oral, descriptions, drawings sketches, photographs, illustrations, diagrams or specifications concerning the goodsmade or given by or for the Seller before contract, whether in catalogues, brochures, leaflets, price lists or otherwise, are for the purpose of informationand guidance only. Where goods are sold by reference to descriptions in a catalogue, the goods are sold subject to the tolerances and variations expressedor implied in the catalogue. In that design and sources of supply may change from time to time the Seller shall have the right to supply goods of altereddesign or from the new source of supply.PRICESAll goods will be supplied at the price and the Purchase Tax rate ruling on the date of dispatch. All prices shown in catalogues, leaflets or brochures, arenet ex works prices exclusive of (Purchase Tax) and delivery charges, and are for guidance only. The Seller reserves the right to vary such prices at anytime in its discretion. Quotations expressed to be for a fixed price remains open for acceptance by the Purchaser within the period stated in the quotationor if no period stated, within 30 days of the date of the quotation irrespective of when the quotation is received by the Purchaser.ACCEPTANCE OF ORDERAll orders are accepted by the Seller subject to these Conditions of Sale unless otherwise varied by agreement in writing. Contracts for delivery byinstallments shall be deemed to be indivisible.THE PRICE1. The Seller may at any time before delivery increase the price of the undelivered balance of the goods by notice in writing to the Buyer. 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Time of payment shall be of the essence and failure by the Buyer to pay the price or any installment thereof in due time shall entitle the Seller to treatsuch failure as a repudiation of the whole Contract by the buyer and to require the Buyer to make immediate payment of all monies due or to becomedue and to recover from the Buyer damages for such breach of contract and/or (at the Sellers option) to charge interest at either five percent per monthor two percent above the Base Rate of LloydsTSB Bank Ltd. whichever shall be the greater, from the due date until payment..DELIVERYUnless otherwise agreed in writing between the Seller and the Buyer the following provisions shall apply.I. Delivery of the Goods shall take place when the Goods shall be delivered at the address specified on the Sellers Quotation, Acknowledgment or otherdocumentation. Were ever possible delivery will be next day or within two days of receipt of the order. 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Notwithstanding delivery and the passing of risk and solely for the purposes of securing payment of all monies due or to become due to the Seller bythe Buyer on any account in the event of the Buyer entering liquidation or having a winding up order made against it or a receiver or receiver and managerbeing appointed of its assets or income or any part thereof or in the event of the Buyer being an individual or individuals he or either of them committingany act of bankruptcy or having any bankruptcy petition presented against him or either of them: II. The property in the Goods shall remain in the Seller until payment in full has been made to the Seller by the Buyer for the Goods and all other sumsdue to the Seller at the date of delivery of the Goods.III. 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Unless otherwise agreed in writing between the Buyer and Seller the Seller may deliver against any order an excess and/or deficiency up to ten percent of weight or volume ordered without any liability whatsoever to the Buyer save that the price shall be adjusted accordingly.

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GLOBAL LIFE SCIENCE SUPPLY

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AGTC Bioproducts t/a National DiagnosticsTel: 01482 646020Fax: 01482 646013Email: [email protected] www.agtcbioproducts.com

Unit 4 Fleet Business Park, Itlings Lane, Hessle, Hull, HU13 9LX

Documents

AGTC Bioproducts Short Price list