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Agilent Vac Elut 12 and 20 Position Manifold Instructions
The Agilent 12- and 20-position extraction manifold features:
• 12-or20-placepositionsforluertipSPEcartridges
• Simpletipdesignallowsforeasyuseofstopcocksordisposableneedletips
• Vacuumgaugewithcontrolandreleasevalvesformonitoringandadjustmentofvacuumlevel
• Achoiceoffourrackstoaccommodateavarietyofcollectionvessels
• Three-pointinterlockingsystemtoensureproperorientationofracks,keyingcartridgepositiontocorrespondingcollectionvessel
• Chemicallyresistant,non-contaminatingconstructionofglass,polyethylene,polypropylene,andDelrincomponents
• Individuallycontrolled,needle-tippedvalves(stopcocks)permitflowcontrolofeachposition
Identifying Parts
Beforeassemblingtheextractionmanifold,ensurethatyouhavetheseparts,picturedinFigure1.
• Glasschamberwithexitvalveinstalled
• WhiteDelrincover*withpolyethylenegasket,vacuumgauge,controlandreleasevalvesinstalled
• Disposableneedletips,long(polypropylene)
• Stopcockvalves
• Luerplugs,male
• Ejectortool
• Rack,12-or20-position,asspecified
*Thethreepostsontheundersideofthecoverserveaselevatedfeetsothecovercanbesetdownwithoutdamagingtheneedletips.Theyalsomaintainspecificalignmentofsamplecartridgeandcollectionvessel.
Using the Extraction Manifold
Atypicalsolidphaseextraction(SPE)procedureconsistsofthefollowingsteps:
1. Condition/equilibratetheSPEcolumnbed.
2. Loadthesample.
3. Washoffinterferences.
4. Eluteanalyte(s)ofinterest.
TheAgilentextractionmanifoldpermitsthesestepstobecarriedoutonuptotwentycartridgesinparallel.
Whensendingalltheeluentstowaste,arackisnotnecessary.ThisisusuallythecaseforatypicalSPEprotocolwhenperformingsteps1through3above.Inthesecaseswasteistypicallydrawnintoaliquidtrapthroughthemanifold’sexitvalvelocatedatthebaseoftheglasschamber.WhenoptimizingortroubleshootinganSPEprotocol,thewastefromthesestepscanbecollectedintubeslocatedintheracksforindividualanalysis.
Figure 1. Vac Elut 20-position unit.
VacuumRelease
(whiteplasticball)
LuerPlugs
DisposableNeedleTips
(protrudingthroughthecoverandintotheglasschamber)
VacuumGauge
ControlValve
AlignmentPegs
GlassChamber
Rack
ExitValve
2
Set up a vacuum system
1. Connecttheoutletsideofaliquidtraptoavacuumsourceusingvacuumtubing.
CAUTION: Makecertainthattheliquidtrapiscompatiblewith,andhassufficientcapacitytocollectallthewastethatwillbegeneratedinyourSPEprocedure.Ifthetrapcapacityistoosmall,wastemaycontaminateand/ordamagethevacuumpump.
2. Connectthemanifold’sexitvalveatthebaseoftheglasschambertothetrapinletusing3/8inidvacuumtubing.
3. Putthecovertopontheglasschamber.Donotinstallarackatthistimeunlesstheneedletipsandstopcocksrequiredforyourprotocolaresecurelymountedinthetopcover.
Installing needle tips and stopcock valves
InstallastopcockwiththevalvehandlepointingoutwardoradisposableneedletipineachofthetopcoverportsasdetailedinthisprocedureandasshowninFigure1.Thismakesthevalveaccessibleforoperation.Thetipsoftheneedlesandstopcockswillextendintocollectiontubesmountedinarackinsidethemanifold.
CAUTION: Youmustfollowthisproceduretoobtainpropersealingofcomponentsmountedinthetopcover.Failuretofollowthisprocedurewillcausejointleaksresultinginvacuumloss.
1. Ifinstalled,removetherackfromthemanifold.
2. Placethetopcoveronthemanifoldandbesureitisproperlyseatedontheedgeoftheglasschamber.
CAUTION: Damagetothetopcoverorrackmayresultiftherackisleftinthemanifoldandthethreealignmentpostsarerestingontherackwhenpressureisappliedtothetopcover.Iftherackmustbeleftinthemanifold,verifythetopcoverisrestingontheglasschamberandnotontherackbeforeproceeding.
3. Insertaneedletipintoahexagonalholeandlightlywiggletheneedlewhilepushingitdowninsidethehexagonalhole.Usingyourthumb,pushtheneedleasfarasitcango.Repeatthisstepfortherestoftheneedletips.Usethissamemethodtoinstallstopcocks.
4. Placeanejectorhandlehorizontallyontopoftwoneedlesandpressdownhardonit.Repeatthisprocedureonalloftheneedles.Alsousethisproceduretosealthestopcockjointtothetopcover.
5. Forneedletipsthatarenotinuse,installaluerplugbypushingitdownintheneedletip’sinlethole.Theluerplugisusedonlywithneedletips.DO NOT INSTALL LUER PLUGS DIRECTLY ON THE COVER!
Removing items from the top cover
1. Removeallliquidfromthecolumns.
2. Toremovealuerplugorcolumn,firsttwistittobreakthefrictionjoint.Whenthepartturnsfreely,liftitout.
CAUTION: Donotpullaplugorcolumnwithatightconnectionstraightupward.Thisforcecancausethejointbetweenthetopcoverandtheneedletiporstopcocktoloosen,resultinginalossofvacuum.
Remove or install a rack of collection tubes
1. Closetheexitvalveor,alternatively,turnoffthevacuumpump.
2. Breakthevacuuminthemanifoldchamberbyslightlyoffsettingthewhiteballfromitsseat(seeFigure2).
3. Removethecoverwhenthevacuumgaugereadsatmosphericpressure.
4. Installorremovearackoftubes.
5. Replacethetopcover.Ifarackisinstalled,carefullyalignthethreepostsonthebottomofthecoverwiththecorrespondingnotchesonthetopplateoftherack.
Manifold setup for protocol
1. Installtheneedletipsandstopcocksrequiredbytheprotocol.
2. Ifrequired,removeorinstallarackoftubes.
3. Putanextractioncartridgeintoeachvalvehubandseatitfirmly.
4. Closeallvalvesbyturningthehandlesuntileachindicatorshowsthatthevalveisintheclosedposition.
5. Setupthevacuumsystemandturnonthevacuumpump.Adjustthecheckvalvetosetthedesiredvacuumlevel.
6. FollowtheSPEprotocolrecommendedforthetypeofcolumnusedandtheapplication.Addfluidintothereservoirsbeforeopeningthestopcockstostartorresumeflow.
7. Openeachcolumn’sstopcockvalvetoevacuatetheliquidasrequiredbytheprotocol.
Figure 2. Breaking the vacuum.
Ballring,unseattobreakvacuum
3. Toremoveaneedletipand/orstopcockvalve,pryitoutcarefullywiththeejectortool.
CAUTION: Pushtheejectortooldownslowlytopreventthelaunchingoftheneedletip.
3
Note:Picturesshownareforillustrationpurposesonly
CORRECTLY SEATED NEEDLEShowing aligned serrations with corners of hexagonal hole
Needle serrated area
NEEDLE INSERTIONHexagon shaped hole on the cover
Figure 3.
Figure 4.
Figure 5.
Figure 6.
4
Ordering Information
Description Part No.
Standard Vac Elut 20 Manifold
VacElutmanifoldwithcollectionrackfor10x75mmtesttubes
12234105
VacElutmanifoldwithcollectionrackfor13x75mmtesttubes
12234100
VacElutmanifoldwithcollectionrackfor13x100mmtesttubes
12234101
VacElutmanifoldwithcollectionrackfor16x75mmtesttubes
12234102
VacElutmanifoldwithcollectionrackfor16x100mmtesttubes
12234103
Racks and replacement parts for Standard Vac Elut 20 Manifold
Standardglassbasin 12234505
20-portcollectionrackfor13x75mmtesttubes 12234507
20-portcollectionrackfor13x100mmtesttubes 12234508
20-portcollectionrackfor16x75mmtesttubes 12234509
20-portcollectionrackfor16x100mmtesttubes 12234510
Replacementlidgasket 12234502
VacElut20lidcover 12234501
Tall Vac Elut 20 Manifold
VacElut20manifoldwithtallglassbasinandcollectionrackfor16x150mmtesttubes,completesystem
12234104
20-portCollectionrackfor10x75mmtesttubes 12234517
Tallglassbasin 12234512
Description Part No.
Vac Elut 12 Manifold
12-portvacuummanifoldprocessingstationandcollectionrackfor16x100mmtesttubes
5982-9110
Racks and replacement parts for Vac Elut 12 Manifolds
VacElut12lidcover 5982-9111
VacElut12sealinggasket 5982-9112
VacElut12glasschamber 5982-9113
12-portcollectionrackfor13x75mmtesttubes 5982-9114
12-portcollectionrackfor13x100mmtesttubes 5982-9115
12-portcollectionrackfor16x75mmtesttubes 5982-9116
12-portcollectionrackfor16x100mmtesttubes 5982-9117
Description Unit Part No.
Common parts and disposables for all Manifolds
Manifoldneedletipejectortool 5982-9105
Polypropylenedeliveryneedles 25/pk 12234511
ManifoldSSneedlewithpolypropylenecoating
25/pk 12234042
Stainlesssteelneedletip 25/pk 12234038
Replacementexitvalveforglassbasin 12234506
Manifoldexitvalvereplacementkit 12234005
Vacuumgaugeassembly 12234504
Manifoldballring/vacuumquickrelease 12234013
Manifoldshortvalvestopcock 15/pk 12131005
Manifoldlongvalvestopcock 20/pk 12234520
Manifoldmaleluerplugs 25/pk 12234518
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Agilentshallnotbeliableforerrorscontainedhereinorforincidentalorconsequentialdamagesinconnectionwiththefurnishing,
performance,oruseofthismaterial.
Information,descriptions,andspecificationsinthispublicationaresubjecttochangewithoutnotice.
©AgilentTechnologies,Inc.,2012PublishedintheUSA,January24,2012
EditionCode04100-06/11PartNumber7704100
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