Agile - affordable - accurate 1 Analyticon Biotechnologies AG Lichtenfels - Germany

agile - affordable - accurate

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Analyticon Biotechnologies AGLichtenfels - Germany


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Basics of Hematology

Hemolyzer ®


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Measuring Principles

Laser light scatter technology for 5-part WBC differential

Impedance measurement for WBC, RBC und PLT

Light absorbance for measuring HGB


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Types of blood cells

RBC: 95% of all blood cells

PLT: ~4.8% of all blood cells

WBC: ~0.2% of all blood cells

NEU: 40-75% LYM: 20-45% MON: 2-8% EOS: 1-6% BAS: 0-1%


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PLT & RBC LYM MON GRAN

RBCs destroyed

WBCs selectively shrank to nuclei

Differential Lysing Process


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& HGB

3-part Differential WBC HistogramPLT & RBC LYM MON GRAN


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Monocytes

Low angle forward scatter

Size

RBC GHOST

Eosinophiles Neutrophiles

Lymphocytes

4-part Differential WBC Scattergram

Basophiles cannot be reliably differentiated from the rest of the white blood cells

Additional optical count is needed


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Hemolyzer® 3


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Hemolyzer® 3

Provides 20 parameters CBC including 3-part WBC differential

3 Histograms: WBC, RBC, PLT

Throughput: 60 samples per hour

6 QC lots, Levey-Jennings diagrams

Database capacity: 10,000

Only 3 reagents (cyanide-free)

Automatic monitoring of reagent status


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Hemolyzer® 3

Sample Volume: 25 μl (open tube sampling)

Automatic clogging prevention

Large, high resolution colour LCD

Easy, intuitive operation

Interfaces: RS232, USB

Printout via internal thermal printer (optional also via external printer)

Compact design:

350 x 490 x 385 mm Weight: 15 kg


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Daily Maintenance

Hemolyzer Hypocleaner is inserted as sample and “measured”

Hemolyzer® 3: Maintenance


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Hemolyzer® 3: Key Benefits

Safe Operation The sample is being aspirated inside the analyzer,

practically excluding the risk of injuries for the user

High speed

Results within 60 seconds

Reliable Instrument

Easy maintenance by the user


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Hemolyzer® 3: CompetitorsModel Hemolyzer® 3 Cell-dyn® 1800 AC•T Diff AC•T Diff2 ABX Micros 60 Distributor Analyticon Abbott Beckman Coulter Beckman Coulter Horiba

WBC diff. 3 3 3 3 3Number of parameters 20 18 18 18 18Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLTThroughput (PT/h) 60 60 50 50 60 (open); 55(closed)Sample volume [μl] 25 30 12 18 10

Open/Closed sampling /- /- /- / / STAT Function Number of reagents 3 3 3 3 3Bar code reader optional optional optional Autoloader (capacity) - - - - - Colour display - - - Touch Screen - - - Internal printer - - - RS-232/USB / /- /- /- /- Database capacity 10,000 10,000 250 250 1; 78 (with smart card) QC Levey-Jennings Levey-Jennings Levey-Jennings, IQAP Levey-Jennings Dimensions Size (WxDxH) [cm]

35x49x38.5 66x44x52 38x42x39 45x49x40 36x42x32

Weight [kg] 15 55 16 20 14Operating temperature 15-35°C 18-30°C 16-34°C


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Hemolyzer® 3: CompetitorsModel Hemolyzer® 3 M 16M/C/20M/C M 16S/20S CA 620 KX 21 Distributor Analyticon Medonic Medonic Medonic Sysmex

WBC diff. 3 3 3 3 3Number of parameters 20 16/20 16/20 9/16/20 18Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLTThroughput (PT/h) 60 65 65 60 60Sample volume [μl] 25 100 (open);

250 (closed)300 125 50

Open/Closed sampling /- / (C only) / /optional /- STAT Function Number of reagents 3 3 3 3 2Bar code reader optional optional optionalAutoloader (capacity) - - (2x20) - - Colour display - -Touch Screen - - - Internal printer - - - RS-232/USB / / / / / Database capacity 10,000 220 300QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Dimensions Size (WxDxH) [cm]

35x49x38.5 29x41x46 33x43x46 42x35x46 42x48x36

Weight [kg] 15 18 22 22 28Operating temperature 15-35°C 18-32°C 18-32°C 18-32°C


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Hemolyzer® 3: CompetitorsModel Hemolyzer® 3 BC 3000+ BC 3200 BC 3600 Distributor Analyticon Mindray Mindray Mindray

WBC diff. 3 3 3 3Number of parameters 20 19 19 21Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLTThroughput (PT/h) 60 60 60 60Sample volume [μl] 25 13 13 20

Open/Closed sampling /- /- -/ /optional STAT Function Number of reagents 3 3 5 3Bar code reader optional optional optional optionalAutoloader (capacity) - - - - Colour display Touch Screen - - - Internal printer RS-232/USB / / / / Database capacity 10,000 35000 35000 40000QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Dimensions Size (WxDxH) [cm]

35x49x38.5 39x46x40 39x46x42 45x40x45

Weight [kg] 15 21 25 28Operating temperature 15-35°C 15-30°C 15-30°C 15-30°C


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Hemolyzer® 3: Competitors

Model Hemolyzer® 3 Celltac α MEK-6400/6410/6420

Mythic 18 H18 light Celly Plus

Distributor Analyticon Nihon Kohden Orphee SFRI Biocode Hycel

WBC diff. 3 3 3 3 3Number of parameters 20 18 18 20 18Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLTThroughput (PT/h) 60 63 60 60 60Sample volume [μl] 25 30 9.8 9.6 10

Open/Closed sampling /- / /- STAT Function Number of reagents 3 4 3 3 3Bar code reader optional optional optional optionalAutoloader (capacity) - - - - -Colour display - Touch Screen - - Internal printer optional optional RS-232/USB / /- / / /Database capacity 10,000 400 1,500 20,000 1,000QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Dimensions Size (WxDxH) [cm]

35x49x38.5 23x45x38.3 25x34x35 33x43x38 32x35.2x36.8

Weight [kg] 15 9 23 9.5Operating temperature 15-35°C 18-35°C 18-35°C


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Hemolyzer® 5


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Hemolyzer® 5

Provides 24 parameters CBC including 5-part WBC differential

2 Histograms: RBC, PLT 2 Scattergrams: 4-diff WBC, BASO

Throughput: 60 samples per hour

6 QC lots, Levey-Jennings diagrams

Database capacity: 100,000

Only 3 reagents (cyanide-free)

Automatic monitoring of reagent status


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Hemolyzer® 5

Sample Volume: 100 μl (open/closed, whole blood)

Automatic clogging prevention

Large colour LCD touch screen

Easy, intuitive operation

Autoloader (100 samples) optional

Interfaces: RS232, USB

Printout via external Microsoft®

WindowsTM compatible printers


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Compact design: 410 x 465 x 515 mm

Weight: 35 kg (with autoloader: 49 kg)

Hemolyzer® 5: Dimensions


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Hemolyzer® 5: Autoloader

Plug-In; can be added any time

Based on linear racks

Up to 100 samples (10 racks)

Cap recognition and cap piercing

Monovette®, Vacutainer®, Vacuette®

Integrated barcode reader

Integrated sample mixer

Using the same sample needle


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Monocytes

Low angle forward scatter

Size

RBC GHOST

Eosinophiles Neutrophiles

Lymphocytes

Hemolyzer® 5: 4-Diff Scattergram


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in the 4-part differential the basophiles cannot be reliably differentiated from the rest of the white blood cells

additional optical count is needed for basophiles

in the WBC/HGB dilution all WBC’s are (over)lysed with the exception of the basophiles

after the impedance count the WBC/HGB dilution is transferred to the optical head for BASO count

Hemolyzer® 5: Basophile Scattergram


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Daily Maintenance

Hemolyzer Hypocleaner is inserted as sample and “measured”

Regular Maintenance (depending on throughput)

Cleaning of measurement head Cleaning of shear valve

Hemolyzer® 5: Maintenance


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Hemolyzer® 5: Key Benefits

Safe Operation The sample is being aspirated inside the analyzer,

practically excluding the risk of injuries for the user

Patented Laser Technology

High speed

Results within 60 seconds

Reliable Instrument

Easy maintenance by the user

Autoloader (100 samples)

Upgrade can be done at any time


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Hemolyzer® 5: CompetitorsModel Hemolyzer® 5 Cell-dyn® 3200 CS Cell-dyn® 3200 AL Cell-dyn® 3700 CS Cell-dyn® 3700 SLDistributor Analyticon Abbott Abbott Abbott Abbott WBC diff. 5 5 5 5 5Number of parameters 24 22 22 23 23Histograms RBC, PLT RBC, PLT RBC, PLT RBC, PLT RBC, PLTScattergrams 4diff (L-M-N-E), BAS WBC, RBC, PLT, RET WBC, RBC, PLT, RET WBC, RBC, PLT, WBC, RBC, PLT,

Throughput (PT/h) 60 71 72 90 90Sample volume [μl] 100 240 (closed); 150 (open) 240 (closed); 150 (open) 240 (closed); 130 (open) 355 (AL); 240 (closed);

130 (open)Open/Closed sampling / / / / /STAT Function Number of reagents 3 4 4 4 4Bar code reader optional Autoloader (capacity) optional (100) - (50) - (100)Standalone - - - -Touch Screen - - - -Internal printer - - - - -RS-232/USB 1/4 /- /- /- /-Database capacity 100,000 10,000 10,000 10,000 10,000QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Dimensions Size (WxDxH) [cm]

40x45x50 83x48x59 83x48x74 76x61x56 76x68x79

Weight [kg] 35 97 106 86 131


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Hemolyzer® 5: CompetitorsModel Hemolyzer® 5 AC•TTM 5 Diff OV/CP AC•TTM 5 Diff AL HmX ABX Pentra (XL) 80Distributor Analyticon Beckman Coulter Beckman Coulter Beckman Coulter Horiba WBC diff. 5 5 5 5 5Number of parameters 24 24 26 26 24Histograms RBC, PLT RBC, PLT WBC, RBC, PLT WBC, RBC, PLT RBC, PLTScattergrams 4diff (L-M-N-E), BAS 4diff (L-M-N-E), BAS 4diff (L-M-N-E) 4diff (L-M-N-E)

Throughput (PT/h) 60 60 60 80 75Sample volume [μl] 100 53 (CBC + diff); 30 (CBC) 53 (CBC + diff); 30 (CBC) 185 (closed); 125 (open) 53 (CBC + diff); 30 (CBC)

Open/Closed sampling / / (CP) / / /STAT Function Number of reagents 3 5 5 5 5Bar code reader optional optional Autoloader (capacity) optional (100) - (100) (100)Standalone (OV)/-(CP) - - Touch Screen - - - Internal printer - - - - (laser)RS-232/USB 1/4 / / / /2Database capacity 100,000 10,000 10,000 5,000 10,000QC Levey-Jennings IQAP IQAP IQAP Levey-JenningsDimensions Size (WxDxH) [cm]

40x45x50 44.4x58x50.1 81.5x58x54 61.5x61.7x84.8 82x57x54cm

Weight [kg] 35 36.2 55 94.5 55


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Hemolyzer® 5: CompetitorsModel Hemolyzer® 5 BC 5300 BC 5380 BC 5500 BC 5800Distributor Analyticon Mindray Mindray Mindray Mindray WBC diff. 5 5 5 5 5Number of parameters 24 27 27 27 29Histograms RBC, PLT 3 3 RBC, PLT RBC, PLTScattergrams 4diff (L-M-N-E), BAS 1 1 4diff (L-M-N-E), BAS 4diff (L-M-N-E), BAS

Throughput (PT/h) 60 60 60 80 (AL); 60 (manual) 90Sample volume [μl] 100 20 (CBC + diff); 15 (CBC) 20 (CBC + diff); 15 (CBC) 180 (closed); 120 (open) 180 (closed); 120 (open)

Open/Closed sampling / /- /- / /STAT Function Number of reagents 3 5 5 6 6Bar code reader optional optional optional optionalAutoloader (capacity) optional (100) - (30) optional (50) optional (50)Standalone - - - -Touch Screen - - Internal printer - - - - -RS-232/USB 1/4 /4 /5Database capacity 100,000 40,000 40,000 40,000 40,000QC Levey-Jennings Dimensions Size (WxDxH) [cm]

40x45x50 41x53x47 66.4x61.3x58.5 66.4x61.3x58.6

Weight [kg] 35 45 73 76


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Hemolyzer® 5: CompetitorsModel Hemolyzer® 5 XS 800i XS 1000i XT 1800i/2000iDistributor Analyticon Sysmex Sysmex Sysmex WBC diff. 5 5 5 5Number of parameters 24 24 24 24/31Histograms RBC, PLT RBC, PLT RBC, PLT RBC, PLTScattergrams 4diff (L-M-N-E), BAS 4diff, WBC/Baso, PLT,

Ret (2000i)Throughput (PT/h) 60 60 60; AL: 52 80Sample volume [μl] 100 20 20 85; 150 (AL)Open/Closed sampling / /- / /STAT Function Number of reagents 3 4 4 5Bar code reader optional optional optional optionalAutoloader (capacity) optional (100) - optional (20) Standalone - - -Touch Screen - - -Internal printer - - - -RS-232/USB 1/4 / / Database capacity 100,000 10,000 10,000 10,000QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-JenningsDimensions Size (WxDxH) [cm]

40x45x50 32x50x41 32x40x41 53x63x50

Weight [kg] 35 24 24 52


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Hemolyzer® 5: CompetitorsModel Hemolyzer® 5 Celltac E MEK-7222 Celltac E MEK-8222 Mythic 22 OT/CT Mythic 22 ALDistributor Analyticon Nihon Kohden Nihon Kohden Orphee Orphee WBC diff. 5 5 5 5 5Number of parameters 24 22 22 22 22Histograms RBC, PLT RBC, PLT RBC, PLTScattergrams 4diff (L-M-N-E), BAS 5-diff 5-diff

Throughput (PT/h) 60 51 80 50(OT)/45(CT)Sample volume [μl] 100 55 (CBC + diff); 30(CBC) 56 (CBC + diff); 30 (CBC) 17 (OT)/20 (CT) 20

Open/Closed sampling / /optional /optional / (CT only) /STAT Function Number of reagents 3 5 5 3 3Bar code reader optional optional optional optional Autoloader (capacity) optional (100) - (50) - (50)Standalone Touch Screen Internal printer - optional optional - -RS-232/USB 1/4 / / 1/2 1/5Database capacity 100,000 100,000 1,500 1,500QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Dimensions Size (WxDxH) [cm]

40x45x50 61.3x58.3x55 25x34x35 48x40x40 38.2x48.5x52

Weight [kg] 35 55 11 25 38


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Agile - affordable - accurate 1 Analyticon Biotechnologies AG Lichtenfels - Germany