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AGARIC DIVERSITY IN THE AGARIC DIVERSITY IN THE GREAT SMOKY MOUNTAINS GREAT SMOKY MOUNTAINS
NATIONAL PARKNATIONAL PARK
PART OF THE GSMNP ALL PART OF THE GSMNP ALL TAXA BIODIVERSITY TAXA BIODIVERSITY INVENTORY (ATBI)INVENTORY (ATBI)
PROJECT GOALS
• Three years of collections by experts in various groups of agaric fungi
• Databasing of current and past collections
• Bar coding of species using ribosomal ITS region
• Species pages available on web
TENN
• Tennessee herbarium web site lists 1500 species from the GSMNP
• Goal – to link to photos and records in next three years
SPONSORSKaren HughesRon PetersenEd Lickey
Gymnopus – 450 sequencesCollybiaOmphalotus Artomyces (Clavicorona)LentinellusLentinulaXerulaRamaria
PARTICIPANTSAime, Cathy – Rusts and CrepidotusAmmirati, Joe - CortinariusBaroni, Tim – EntolomaBuyck, Bart – Russula / Lactarius Caranza, Julietta - TrametesCifuentes, Joaquin - GeneralHalling, Roy – Gymnopus and othersHorak, Egon - GeneralKerrigan, Rick - AgaricusLodge, Jean - HygrophoraceaeMata, Juan Luis - GymnopusMatheny, Brandon - InocybeMcCleneghan, Coleman - PholiotaMethven, Andrew - LactariusOvrebo, Clark - TricholomaTulloss, Rod -AmanitaVerbaken, Anameka – Russula /Lactarius
COVERAGE
Andrew Miller - Pyrenomycetes
TWO PROBLEMS FOR BARCODING USING THE ITS REGION
PROBLEM 1
INABILITY TO DISTINGUISH SOME APPARENT MORPHOSPECIES (GYMNOPUS SECT LEVIPEDES)
• But – does distinguish a number of Gymnopus collections previously grouped as the same morphospecies (cryptic species)
TWO PROBLEMS FOR BARCODING USING THE ITS REGION
PROBLEM 2
THE ITS REGION IN COLLECTIONS FROM THE GSMNP HAS RELATIVELY HIGH LEVELS OF HETEROZYGOSITY FOR INDELS.
•Requires cloning with its attendant problems
Cortinarius sp. = 31.3%
Lactarius sp. = 25.0%
Hygrophorus = 57.7%
Saprobes = 28.5%
PROPORTION OF COLLECTIONS FROM THE GSMNP THAT WERE ALREADY REPRESENTED IN GENBANK
[98% CONGRUENCE WAS ARBITRARILY CONSIDERED TO BE CONSPECIFIC]
• 159/533 MATCHED SPECIES IN GENBANK• 23/533 MATCHED UNNAMED ENVIRONMENTAL SAMPLES
• 351/533 HAD NO MATCH IN GENBANK
FOR AGARICS, SEQUENCE COVERAGE IS STILL SIGNIFICANTLY INCOMPLETE
GSMNP BAR CODING PROJECT?
• Home of an ATBI
• Center of Biodiversity
• Highly varied terrain /ecosystems
• Nice collecting spot
• Good field station
• On the ground help
• Need I say more?