The Dako FLEX RTU solution Ensures optimal staining results, slide after slideUsing validated protocols and optimized reagents reduces the risk of false negative or false positive results. A robust, specific and sensitive IHC assay is critical for providing staining results that support an accurate patient diagnosis. Dako FLEX RTU is developed in collaboration with pathology experts to ensure optimal staining results.

Advanced Staining Dako FLEX RTU

DATA SHEET

Plug and play on Dako advanced staining platforms

�� Pre-diluted and carefully selected for specificity and sensitivity

�� Proven concept since 2007�� Excellent antibody quality -

confirmed by various EQA schemes

�� Broad portfolio of IVD antibodies

FLEX RTU antibodies

�� Proven visualization with low complexity and very high sensitivity (Skaland et al. 2009)

�� Visibility of antigen in high and low expressing tissue

�� Kit configuration provides – Flexibility – Consistency – Simplicity

EnVision™ FLEX visualization system

Validated protocols

� Automated protocols developed for Dako solutions

� Optimized, validated protocols developed in cooperation with leading pathologists

� Robust protocols compensate for pre-analytical variations

The solution consists of:

�� Pre-diluted antibody — FLEX RTU antibody�� Visualization system — EnVision™ FLEX/FLEX+ �� Optimized and validated protocol — the recipe for consistent high-quality results

Dako protocols provide consistent high-quality resultsThe most important element in the qualification of the staining results of clinical samples is accurate selection and staining of control tissue.

Dako protocols are the result of a comprehensive study of numerous different tissue types, tissue thicknesses, protocol-step durations, target-retrieval methods, antibody dilutions, and pre-analytical variations.

The Dako optimized protocol ensures that every test performance is highly robust, accurate and consistent, compensating for variations in pre-analytical parameters to provide diagnostic certainty slide after slide.

Find the most up-to-date range of FLEX RTU antibodies for all of our advanced staining platforms at www.dako.com/products

2906

9 15

AU

G13

Australia +61 3 9357 0892

Canada +1 905 335 3256

France +33 1 64 53 61 44

Japan +81 3 5802 7211

Poland +48 58 661 1879

United Kingdom +44 (0)1 353 66 99 11

Austria +43 1 408 43 34 0

China +86 21 3612 7091

Germany +49 40 69 69 470

Korea +82 2 402 6775

Spain +34 93 499 05 06

United States of America +1 805 566 6655

Belgium +32 (0) 16 38 72 20

Denmark +45 44 85 97 56

Ireland +353 1 479 0568

The Netherlands +31 20 42 11 100

Sweden +46 8 556 20 600

Brazil +55 11 50708300

Finland +358 9 348 73 950

Italy +39 02 58 078 1

Norway +47 23 14 05 40

Switzerland +41 41 760 11 66

www.dako.com

Represented in more than 100 countries

Corporate Headquarters Denmark +45 44 85 95 00

Non-optimized incubations

Pre-analytical variation

Pre-analytical variation

IHC protocols with unsaturated incubations increase total output variation

IHC protocols with saturated incubations minimize total output variation

Analytical variation

Analytical variation

Total variation

Total variation

Optimized incubations

Dako recommended protocols ensure optimal incubations to maximize signal transfer (right). This minimizes the total output variation in the IHC test.