Embed Size (px)
Citation preview
Adhesion of Human Lens Epithelial Cells on Hydrophilic Acrylic IOLs Coated With
Polyethylene Glycol in Culture
Authors: Antonio Carlos Lottelli Rodrigues, Magda Massae Hata Authors: Antonio Carlos Lottelli Rodrigues, Magda Massae Hata Viveiros, Ricardo Torres Soares, Nilson C. Cruz, Michelle Sako Viveiros, Ricardo Torres Soares, Nilson C. Cruz, Michelle Sako
Omodei, Silvana Artioli Schellini, Cláudia Aparecida RainhoOmodei, Silvana Artioli Schellini, Cláudia Aparecida Rainho
The authors have no financial interest in the subject matter of this posterThe authors have no financial interest in the subject matter of this poster
[email protected][email protected]
[email protected][email protected]
Botucatu Medical School, UNESP - São Paulo State University - Brazil
Support:
Introdution
• The posterior capsular opacification (PCO) remains a frequent complication of cataract surgery ranging from 3.5% to 55.6%, depending on the design and material of the intraocular lens.
• Studies on improved IOL biomaterial have focused on treatments that Studies on improved IOL biomaterial have focused on treatments that are able to potentialize the inhibition of cell adhesionare able to potentialize the inhibition of cell adhesion
• One of this treatment the polymerization of the acrylic hydrophobic surface IOLs with polyethylene glycol. This kind of treatment has shown a significant reduction in protein deposition and cell adhesion in the IOL (Lee et al., 2007)
Purpose
To evaluate the ability of cell adhesion in the acrylic the ability of cell adhesion in the acrylic
IOLs coated with hydrophilic polyethylene glycol IOLs coated with hydrophilic polyethylene glycol
(threated group) and uncoated IOLs (control group) (threated group) and uncoated IOLs (control group)
in crystalline epithelial cells human culture.in crystalline epithelial cells human culture.
Methods
• The research was Approval by the Botucatu Medical School
Ethics committee.
• We performed the epithelial cells culture from the anterior
capsule of 144 patients undergoing cataract extraction by undergoing cataract extraction by
phacoemulsification in the Botucatu Medical School.phacoemulsification in the Botucatu Medical School.
• Two patterns of cells growth in culture were observed, a Two patterns of cells growth in culture were observed, a
dendritic one and a rounded one.dendritic one and a rounded one.
Patterns of cell growth:
Round pattern Dendritic pattern
Methods
• When reaching the semi-confluence, five rounded pattern When reaching the semi-confluence, five rounded pattern epithelial cell cultures and five dendritic pattern cell cultures were epithelial cell cultures and five dendritic pattern cell cultures were trypsinizedtrypsinized
• 3,000 cells of each culture was seed on the Hydrophilic IOLs (Ioflex®, Mediphacos, Belo Horizonte - Brazil) untreated and treated with polyethylene glycol, both the samples in triplicate.
• The treated IOLs were submitted to treatment with polyethyleneglycol (PEG 300) 392°F evaporated to a glass chamber at a pressure of – 0.0045 Torr, using radio frequency energy 5 to 15W for 30 minutes.
Methods
• IOLs were left at 98,6° F in a humidified incubator with 5% CO2 for 2 hours for cell adhesion to occur .
• Then nutrient with 20% fetal bovine serum was added, and the cultures kept in the incubator for 24 hours.
• The IOLs were washed three times with PBS to remove The IOLs were washed three times with PBS to remove the loose cells .the loose cells .
• Then the IOLs were them trypsinized to detach adhered cells. • The number of cells removed from which IOL was counted
with hemocytometer.
Methods
• 126 (87.5%) cultures out of 144, showed the rounded pattern, and 18 (12.5%) the dendritic pattern.
group group
GERAL GERAL
Round patternRound pattern Dendritic pattenDendritic patten
Treated Treated 1000 (0; 2000) 1000 (0; 2000) 1000 (0; 2000) 1000 (0; 2000) 640 (0;1000) 640 (0;1000)
Control IOLControl IOL 2000 (0; 2000) 2000 (0; 2000) 1000 (1000; 1000 (1000; 2000) 2000)
2000 (0; 2000) 2000 (0; 2000)
value (p) value (p) p<0,05 p<0,05 p=0,421 p=0,421 p=0,095 p=0,095
Results
Table 1: Mean values and minimum and maximum number of cells per group and cell type.
• Overall, there was less cell adhesion on IOLs treated compared to untreated controls, with statistical significance (p <0.05).
• There was no statistical difference in samples of round There was no statistical difference in samples of round pattern; however, there is a tendency for greater pattern; however, there is a tendency for greater adherence in untreated IOLs (p = 0.421).adherence in untreated IOLs (p = 0.421).
• Samples of dendritic cells also showed no statistical difference, but with a significant trend toward greater compliance in untreated IOLs (p = 0.095).
Results
• By using primary cell cultures, it was possible to add a new variable (cell type) to the experiment, which allowed us to get closer to the real situation. The finding of two distinct types of cells was not expected
• Analysis of the two cell pattern revealed their distinct behavior. Although no statistical difference was found, the dendritic pattern seems to be more adherent in comparison with the round pattern, and the adhesion inhibition in the treated IOLs group was higher in the dendritic pattern in comparison with this control group.
Discussion
• These findings lead us to suppose that patients with dendritic pattern cells in culture, were more likely to develop PCO because this kind of cells could be transformed into activated myofibroblasts.
• We could not find in the literature experiments that cite and compare these two types of cells in culture, since only cell
strainsstrains were used for this kind of study.
Discussion
• The coating of the IOLs with polyethylene glycol was effective in The coating of the IOLs with polyethylene glycol was effective in
inhibiting cell adhesion on treated IOLs when compared to inhibiting cell adhesion on treated IOLs when compared to
untreated controls (p <0.05). untreated controls (p <0.05).
• The unexpected finding of two patters of human lens epithelial The unexpected finding of two patters of human lens epithelial
cells in culture opens new perspective for studies on this subject.cells in culture opens new perspective for studies on this subject.
Conclusion
