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18693. 18645. 18709. 18893. Additional data file 5A. 18693. 18645. 18709. 18893. 5B. 18693. 18645. 18709. 18893. 5C. 18693. 18645. 18709. 18893. 5D. 18693. 18645. 18709. 18893. 5E. - PowerPoint PPT Presentation
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18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
Mass (Da)
B6 M1
B6 M2
Rel
ativ
e in
tens
ity
B6 M3
B6 M4
B6 M5
Additional data file 5A
18645 1870918693
18893
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
Mass (Da)
B6 F1
B6 F2
B6 F3 R
elat
ive
inte
nsity
B6 F4
B6 F5
B6 F6
B6 F7
B6 F8
5B
18645 1870918693
18893
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
Mass (Da)
BALB/c M1
BALB/c M2
BALB/c M3
Rel
ativ
e in
tens
ity
BALB/c M4
BALB/c M6
BALB/c M5
5C
18645 1870918693
18893
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
Mass (Da)
BALB/c F1
BALB/c F2
BALB/c F3
Rel
ativ
e in
tens
ity
BALB/c F4
BALB/c F5
BALB/c F6
BALB/c F7
5D
18645 1870918693
18893
5E
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
18600 18700 18800 18900 19000 19100 19200
0.0
0.5
1.0
129S5 M1
129S5 M2
129S5 F1
Mass (Da)
Rel
ativ
e in
tens
ity
129S5 F2
18645 1870918693
18893
Additional data file 5. Individual variation in ESI-MS mass spectra of MUP isoforms in urine. Urine samples from multiple individuals from each strain/sex were analysed by intact mass spectrometry, and the individual spectra are displayed from 18600 Da to 19190 Da. Dotted lines locate the commonly observed mass corresponding to specific proteins. (A) male B6 (n = 5); (B) female B6 (n = 8); (C) male BALB/c (n = 6); (D) female BALB/c (n = 7); (E) male (n = 2) and female (n = 2) 129S5. The spectra are scaled to the base peak intensity (the highest peak in the whole mass spectrum = 1).
