Becausehigh-contentdataismultiparametricinnature,anumberofadvancedanalysistoolsfordataminingareeasilyaccessibleinAcuityXpressSoftware.FromK-Means,K-Medians,HierarchicalClusteringtoSelf-OrganizingMapsandmore,cellscanbeclusteredbyphenotypesandcompoundsbyresponses.Interactiveimagezoomallowsdrilldowntotheoriginalimagestovalidatetheresults.Withinteractiveimagezoom,itisalsopossibletoselecttheimagedatatoanalyzespecificpopulationsofcellsorcompounds.

Interactivescatterplotsofanymeasurementagainstanyother,withinorbetweenplatescanbeeasilycreated.ThearrayofstatisticaldataincludesZ',p-values,correlationcoefficientsandprincipalcomponentscores.

COMPLETE SOLUTION FOR High-content DATA ANALYSIS AND VISUALIZATION

AcuityXpress™SoftwareprovidescellularinformaticsfortheCompleteImagingSolutionfromMolecularDevicesandhasbeenspecificallydesignedtoaddresstheneedsofhigh-contentdataanalysisatanenterpriselevel.AcuityXpressSoftwaresharestheMDCStore™DatabasewithMetaXpress®Softwareforseamlessintegrationofimageacquisition,imageanalysisandinformatics.Thisintegrationenablesdirectlinkageofdataanalysiswiththeoriginalimages.

TheinteractiveimagezoomfeatureinAcuityXpressSoftwaretakesvisualizationofhigh-contentdatatothenextlevel.Thisuniqueandground-breakingtoolallowsfordirectinteractionofimageandnumericaldataatalllevelsandinbothdirections—fromthedetailedviewoftheindividualhigh-resolutionimagestotheglobal,high-levelviewofalloftheimagedatainalargescreen.(Seetheinteractiveimagezoomdrilldownviewsonpage3.)

Curve-fittingforIC50andEC50calculationcanbegeneratedwithpredefinedfunctions,aswellascustomfunctionswithuptosixindependentvariablesand24fittedparameters.Anumberofsearch,minimizationandweightingmethods,andparameterseedingandmodelcomparisonareavailablefortheverybestfits.

Benefits

•Interactive image zoom drill down enables bidirectional interaction between images and numerical data

•Flexible and easy-to-use high-end data mining tools

•Fully integrated with the complete imaging solution

• Open API for easy read/write access to the database

AcuityXpress High Content Informatics Software

The interface in AcuityXpress Software displays a number of different views of the same data set. All views are linked

together. A: cluster view; B: 3-D scatter plot of principal components analysis results; C: analysis of dose-response

using curve fitting (left) or multi-parametric analysis (right);

D: analysis 2-D scatter plots.

AcuityXpress High Content Informatics Software interface

InteractionwiththedatabaseiseasyandcompletewithasimplewizardforthecreationofadvanceddatabasequeriesandanApplicationProgrammingInterface(API)toallowwritingtoandreadingfromthedatabase.Visualizeddatacanbeexportedforpublicationqualityimages.Plateannotationscanbemanagedplatebyplate

A

B

C

D

withthegraphicalPlateLayoutManagerorfromtextfilesorthroughtheAPI.Finally,visualizationscanbeexportedforpublicationqualityimages.

Interactive image zoom brings visualization of high-content data to the next level by allowing direct interaction of image and numerical data at all levels and in both directions—from the detailed view of the individual high-

resolution images to the global, high-level view of all of the image data in a large screen.

AcuityXpress Software interactive image zoom drill down feature

Computer requirementsMinimum client•IBM-compatible32-bitcomputerwitha2.6GHzorfasterIntelPentiumprocessor

•MicrosoftWindowsXPoperatingsystem

•512MBsystemmemory(RAM);morememorymayberequiredforprocessinglargeimagedatasets

•500MBavailableharddiskspaceforclientprograms

•2GBavailableharddiskspacefordatabaseandlocalimagestorage

•32-bitgraphicsdisplay

•2USBportsforsoftwaresecuritykeys

•1CDdrive

Recommended client•IBM-compatible32-bitcomputerwitha3.2GHzorfasterIntelXeonprocessor

•MicrosoftWindowsXPoperatingsystem

•4GBsystemmemory(RAM)

•100GBormoreavailableharddiskspacefordatabaseandlocalimagestorage

•Harddrivesshouldhave16MBcache

•32-bitgraphicsdisplay,256MBvideoRAM,dualmonitoroption

•2USBportsforsoftwaresecuritykeys

•GigabitEthernetcard

•1CD/DVDwriter

Server specificationsPleasediscussyourserverrequirementswithyourcomputervendor.Serverspecificationsdependstronglyonthenumberofsimultaneoususerstosupport.Werecommendthefollowing:

Database requirementsOneofthefollowingdatabaseserversoftwarepackages:•MicrosoftSQLServer2000SP3orlater,Standard,Enterprise,DatacenterandPersonalEditions

•AcuityXpressandMetaXpressSoftwareversions2.0andabovearealsocompatiblewithMSSQLServer2005editions

•Oracle9iRelease2orOracle10g

Minimum database server•IBM-compatible32-bitcomputerwitha2GHzorfasterIntelPentium4orXeonprocessor

•Operatingsystemsupportedbydatabaseapplicationinuse

•1GBsystemmemory(RAM)

•100GBavailableharddiskspace

•2USBportsforsoftwaresecuritykeys

Recommended database server•IBM-compatible32-bitcomputerwithdual2.6GHz(orfaster)Xeonprocessors

•MicrosoftWindowsXPorServer2003operatingsystem

•1GBormoresystemmemory(RAM)

•1TBormoreavailablespaceonaSATAorSCSIRaid0,5,or10storagesub-system

•GigabitEthernetcard

•2USBportsforsoftwaresecuritykeys

Recommended ACE image file server•IBM-compatible32-bitcomputerwith2GHzorfasterIntelPentiumprocessor

•MicrosoftWindowsXPorServer2003operatingsystem

•1GBormoresystemmemory(RAM)

•1TBormoreavailablespaceonaSATAorSCSIRaid0,5,or10storagesub-system

•GigabitEthernetcard

Forbestthroughput,itisrecommendedthattheclient,databaseandimagefileserverbelocatedonseparatecomputers.

Ordering informationAcuityXpressHighContentInformaticsSoftwarePartNumber:9500-0105

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. ©2012 Molecular Devices, LLC • 3/12 • Printed in USA • PN: 0120-1448.D

Patents: http://www.moleculardevices.com/productpatents

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