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Aspergillus oryzae MTCC 5341, when grown on wheat bran as substrate,produces several extracellular acid proteases

!roduction o" the #a$or acid protease %constituting 34& o" the total' by solid(state "er#entation is opti#ized )pti#u# operating conditions obtained aredeter#ined as p* 5, te#perature o" incubation o" 3+C, de"atted soy our

addition o" 4&, and "er#entation ti#e o" 1-+ h, resulting in acid proteaseproduction o" ./4 0 1+5 2g bran

)pti#u# "actor levels are p* 54, incubation te#perature o" 31C, 44&de"atted soy our addition, and "er#entation ti#e o" 1-3 h to yield a #axi#u#activity o" .03 0 1+5 2g bran

!roteases occupy a central position in co##erce, accounting "or nearly /5& o" the global enzy#e #aret They are used extensively in the detergent, leather,phar#aceutical, and "ood industries

ood applications o" proteases include their use in cheese(#aing, beer

clarication, protein hydrolysate production, phar#aceutical, and cos#eticindustries

Acid proteases nd application in the production o" seasoning #aterials, proteinhydrolysates, "er#entation o" soy sauce, and as digestive aids

ila#entous "ungi are exploited "or the production o" industrial enzy#es due totheir ability to grow on solid substrate and produce a wide range o" extracellular enzy#es

several reports have appeared recently about isolation o" acid proteases "ro#di6erent "ungi, Aspergillus oryzae is an organis# o" choice, due to its generallyregarded as sa"e %78A9' status

opti#ize "actors inuencing acid protease production by using 8esponse(sur"ace

#ethodology %89M' As a part o" purication and characterization o" proteases"ro# A oryzae MTCC 5341, sodiu# dodecyl sul"ate polyacryla#ide gelelectrophoresis %9:9;!A7<' and zy#ogra# conr# the release o" onedo#inant acid protease

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=a Metodolog>a de 9upercies de 8espuesta es un con$unto de t?cnicas

#ate#@ticas y estad>sticas utilizadas para #odelar y analizar proble#as en losue una variable de inter?s es inuenciada por otras <l ob$etivo es opti#izar lavariable de inter?s <sto se logra al deter#inar las condiciones Bpti#as deoperaciBn del siste#a