Accelerate Protein Sample Preparation and Analysis · chromatography resins that exhibit high resolution and binding capacities with low non-specific binding for the purification

Accelerate Protein SamplePreparation and Analysis

www.pall.com/lab

Products for Proteomics, BiopharmaceuticalResearch, Diagnostics, and Protein Chemistry

2

Reliable sample preparation and fractionation processes are

key to the success of proteomic research. Effective steps

can facilitate the discovery of life-saving and life-enhancing

diagnostic and pharmaceutical products. Ineffective steps

can lead to loss of valuable samples and time.

Pall Life Sciences manufactures membranes and

chromatography resins that exhibit high resolution

and binding capacities with low non-specific binding

for the purification and concentration of proteins. We

incorporate these media into devices that use fast, gentle

methods and minimize handling to protect your samples.

Our attention to product quality and performance is

expressed in reproducible, reliable results.

Pall also offers a variety of products for protein detection

including PVDF and nitrocellulose membranes for western

blotting, available as cut membranes or integrated into

multi-well filter plates.

Inspiring Confidence in Critical Proteomic Steps

OptimizedPall offers the largest selection of membrane andchromatography resin chemistries and manufactures these under precise, highly controlled conditions to ensure product quality. Applying a unique blend of LeanManufacturing and Six-Sigma principles, our qualityassurance procedures result in superior products withexceptional lot-to-lot reproducibility. Pall is one of the fewcompanies to control device manufacturing through allstages, from media production to housing material selectionto final device assembly. This gives us a distinct qualityadvantage, allowing us to maximize processing accuracy,speed, safety and reliability.

“The results of my work can change people’s lives.That’s why I count on Pall technologies.”

www.pall.com3

Global reach

Pall Corporation is the largest filtration, separation and

purification company in the world. Our diversity and global

reach provide us with unique application insights and

product development opportunities that we use to benefit

our customers. Pall scientific laboratories worldwide work to

solve complex customer problems, test products, explore

new product applications, and provide ongoing technical

support. Our global manufacturing facilities and distribution

networks ensure product availability, easy ordering, and

fast delivery.

Personalized solutions

With over 50 years of experience, Pall knows how to

combine the optimal materials to improve performance in

a range of applications. Our goal is to provide you with the

best products for your application and help you use them

to their fullest potential. Although we offer thousands of

standard product configurations, Pall product teams

work diligently with our clients and other industry

leaders to develop leading-edge technologies and

optimize product configurations.

Scaleable

Whether you are processing a single sample or detecting

thousands of samples, Pall offers a variety of device

configurations to support your techniques. And when

scale-up is a factor, Pall offers product platforms that

incorporate the same membranes and materials of

construction to allow precise scale-up of processing

volumes from lab to process scale.

Automation compatible

Pall’s high throughput products meet industry guidelines

to ensure compatibility with all standard robotic equipment.

Our filter plates meet the ANSI/SBS X-2004 specifications

for smooth operation and worry-free performance. We

partner with industry-leading equipment manufacturers

to develop products and optimize the automation for

both routine and unique applications.

“When we need support, Pall has theresources that link service to science.”

ARRAYS (Pages 13, 29)

MULTI-WELL FILTER PLATES (Pages 14 - 17)

CENTRIFUGAL DEVICES/SPIN FILTERS (Pages 18 - 19)

PROTEIN PURIFICATION KITS (Pages 20 - 21)

TANGENTIAL FLOW FILTRATION PRODUCTS (Pages 22 - 25)

PREFILTRATION/CLARIFICATION (Pages 26 - 27)

DETECTION PRODUCTS (Pages 28 - 29)

CHROMATOGRAPHY PRODUCTS (Pages 8 - 13)

Ultrogel® AcA, Trisacryl® GF Size Exclusion Chromatography Resins

Ceramic HyperD® Ion Exchange Chromatography Resins

Trisacryl, HyperD, HyperCel™ Affinity Chromatography Resins

SDR HyperD Solvent-Detergent Removal Resins

MEP HyperCel Hydrophobic Charge Induction Chromatography Resins

Mustang® Ion Exchange Membrane Devices

Omega™ Ultrafiltration Membrane for Size Exclusion

AcroPrep™ Plates with Hydrophilic Filtration Membranes

AcroPrep Plates with Ion Exchange Membranes

AcroPrep Plates with Prefilters

AcroPrep Plates with Ultrafiltration Membranes for Size Exclusion

AcroPrep Plates with Glass Fiber

AcroWell™ Plates with Binding Membranes

Microfiltration Centrifugal Devices

Ultrafiltration Centrifugal Devices

Enchant™ Kit for Albumin Depletion

Enchant Kits for IgG Purification

Enchant Multi-Protein Affinity Separation Kit

Minimate™ TFF Capsules

Ultrasette™ Lab TFF Devices

LV Centramate™ Lab TFF Systems

Centramate Lab TFF Systems

Acrodisc® Syringe Filters with Prefilters

VacuCap® Vacuum Filtration Devices

Vivid™ Gene Array Slides

FluoroTrans® PVDF Membranes

BioTrace™ PVDF Membrane

BioTrace NT Nitrocellulose Membrane

UltraBind™ Affinity Membrane

Immunodyne® ABC Membrane

Protein Chip System Based on Arrays

Vivid Gene Array Slides

4

Sample complexity reduction is an important first step to facilitate access to

the low abundant proteins of interest for disease research and diagnostics.

The process for human serum and plasma frequently includes depletion of

highly abundant proteins such as albumin and IgG in combination with other

fractionation technologies prior to 2D-Gel or LC-MS/MS separation. This can

be achieved with a combination of specific affinity ligand-based depletion and

ion exchange chromatography-based fractionation technologies. Pall provides

separation technologies and devices to address these challenging needs in

both low and high throughput applications.

Addressing the Challenges ofProtein Sample Preparation

“Sample preparation...it’s the last thing I want to worry about but the first thing on my mind. That’s why I trust Pall.”

Application guide

Sample Preparation Sample DetectionOptimization

www.pall.com

† In combination with appropriate chromatography resin.

5

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6

Pall has thousands of media chemistries from which to

select, and we are constantly modifying and developing

new chemistries to meet your specific application

challenges. Combined with superior housing materials

in a multitude of configurations and industry-leading

research, development, and manufacturing resources,

Pall is uniquely positioned to deliver products exactly

to your specifications.

Finding the Perfect FitFor Your Application

Versatile separation technologies

Pall gives you more filtration and separation material

options than any other organization in the world. This

means you have the best technologies available to optimize

your application performance. Our quality control procedures

result in the production of media with exceptional lot-to-lot

reproducibility and uniformity to give you the consistent,

accurate results you require.

Reliable device configurations

While the heart of Pall is media development and

manufacturing, the design and manufacture of devices

is the ultimate expression of Pall technologies. This is

where it all comes together – high performance media,

outstanding housing materials, and devices designed to

maximize processing accuracy, speed, safety, reliability,

and ease of use.

The charts on pages 5 and 7 will help you evaluate

media and device options based on your applications

and desired material characteristics.

www.pall.com7

Characteristic

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Membrane Name Medium Recommended Applications

Bio-Inert® Membrane Modified Nylon Hydroxyl • • • Filtration of protein solutions, low protein binding,general filtration, clarification of cell lysate and tissue homogenates

Fluorodyne® II Membrane PVDF Hydroxyl • • • • Filtration of low concentration proteins,sample preparation, general filtration

GH Polypro Membrane Polypropylene Proprietary • • • • Time-resolved fluorescence, bead-based assays,(GHP) fluorescent detection of analytes, sample prep

prior to HPLC, general filtration of aqueous and organic solvents

HT Tuffryn® Membrane Polysulfone Proprietary • • • Proven performance in documented procedures

LoProdyne® LP Membrane Nylon Hydroxyl • • • • Filtration of low concentration proteins,sample preparation

Pallflex® Media Glass Fiber Varies • • • • • Prefilters, DNA extraction

PTFE Membrane PTFE PTFE • • • • Vent filters, chemical and molecular synthesis

Supor® Membrane Polyethersulfone Proprietary • • • • General filtration applications, low protein binding,bead-based assays

Supor R Membrane Polyethersulfone RepelTM Treated • • • Vent filters

Versapor® Membrane Acrylic Copolymer Proprietary • • • General filtration applicationson a Nonwoven Support

Versapor R Membrane Acrylic Copolymer Repel Treated • • • Vent filterson a Nonwoven Support

OmegaTM Membrane Modified Proprietary • • • • • Sample preparation, PCR cleanup, sequencing Polyethersulfone cleanup, ultrafiltration separations, nucleic acid and

protein purification, concentration and fractionation

BioSepra® Size Exclusion Varies • • Fractionation, purification, desaltingResins (Ultrogel®, Trisacryl®)

BioSepra Q Ion Exchange Composite Quaternary • •Resins (HyperD®) Material (ceramic) Amine

BioSepra S Ion Exchange Composite Sulfopropyl • •Resins (HyperD) Material (ceramic)

BioSepra DEAE Ion Composite Diethylaminocthyl • •Exchange Resins (HyperD) Material (ceramic)

BioSepra CM Ion Exchange Composite Carboxymethyl • •Resins (HyperD) Material (ceramic)

BioSepra Affinity Resins Varies Varies • • Abundant protein removal, lgG purification,(Blue Trisacryl M, Protein A Ceramic glycoprotein enrichment, lipoprotein purificationHyperD F, Heparin HyperD M,Lysine HyperD, IMAC HyperCel™)

BioSepra Solvent-Detergent Composite Hydrophobic • • Detergent removalRemoval Resins (SDR HyperD) Material (silica) Polymer Moiety

BioSepra Hydrophobic Charge Cellulose Polymer 4-Mercapto- • • Purification of poly and monoclonal antibodies of Induction Chromatography ethyl-pyridine various species, enzymes and recombinant proteinsResins (MEP HyperCel)

Mustang® E Membrane Polyethersulfone Quaternary • • • Removes endotoxin from buffers, water, neutral (positively-charged) Ammonium sugar solutions, and certain biological solutions

Mustang Q Membrane Polyethersulfone Quaternary • • • • • Strong anionic exchanger for DNA clearance,(positively-charged) Amine nucleic acids and negatively-charged proteins,

viral particle purification/concentration

Mustang S Membrane Polyethersulfone Sulfonic Acid • • • • Strong cationic exchanger for positively- (negatively-charged) charged proteins, viral particle

purification/concentration

Biodyne® A Membrane Nylon 6,6 • • • • Macroarrays, microarrays, Southern blots,(amphoteric) northern blots, dot blots, reverse dot blots, DNA

fingerprinting, colony and plaque lifts, ELISA

Biodyne B Membrane Nylon 6,6 Quaternary • • • Macroarrays, microarrays, Southern blots,(positively-charged) Ammonium northern blots, dot blots, reverse dot blots, DNA

fingerprinting, binds negatively-charged molecules

Biodyne C Membrane Nylon 6,6 Carboxyl • • Reverse dot blots, ELISA,(negatively-charged) binds positively-charged molecules

Biodyne Plus Membrane Nylon 6,6 Quaternary • • • Macroarrays, microarrays, Southern blots,(positively-charged) Ammonium northern blots, dot blots, DNA fingerprinting, ELISA

BioTraceTM NT Membrane Nitrocellulose • • • • Western blots, colony/plaque lifts,Southern blots, protein/nucleic acid dot blots,flow-through diagnostic tests, northern blots

BioTrace PVDF Membrane PVDF • • Western blots, protein dot blots

FluoroTrans® PVDF Membrane PVDF • • N-terminal protein sequencing, lowest levels of autofluorescence

FluoroTrans W Membrane PVDF • • Western blots, Southern blots

Immunodyne® ABC Membrane Modified Nylon Proprietary • • • • Oligonucleotide arrays, reverse dot blots,Activated protein arrays, immunoassaysSurface

Leukosorb® Membrane Proprietary Proprietary • • Leukodepletion, nucleic acid extraction,in situ PCR

UltraBindTM Membrane Modified Aldehyde • • Affinity chromatography,Polyethersulfone ELISA, ELISPOT(unsupported)

SurfaceChemistry

Protein purificationand analysis media

Separation Technologies

Microfiltration

Ultrafiltration

Binding

Chromatography

Protein concentration, protein fractionation,contaminant removal, separation based on charge

8

portfolio and expands our offering to include resins. The

BioSepra line of chromatography resins greatly simplifies

protein purification and fractionation. These broad lines of

chromatography products exhibit superior performance

and are useful for affinity, ion exchange, size exclusion,

and hydrophobic interaction chromatography (HIC).

Unique mixed-mode BioSepra products also exist to

provide solutions to current sample preparation challenges

such as detergent removal and antibody purification.

Special features

True Scalability

The resins Pall offers for small-scale discovery

applications are the same ones offered to our

customers currently manufacturing biopharma-

ceuticals. The ability to scale up is essential for

those working in drug discovery, development, and

manufacturing. These resins can be used in varying size

chromatography columns, as well as in batch mode for

single prep or high-throughput mode. This is ideal for

quick preps or in situations where optimizing purification

conditions is required.

Versatile Product Line

Pall bottled resins can be used for small and large sample

sizes involving single use or high throughput methods of

purification. Our base resin varies depending on targeted

applications. These resins can be used in combination

with Pall device configurations such as multi-well filter

plates and spin devices.

High Binding Capacities and Fast Flow Rates

By tailoring attributes such as chemistry, pore size,

and resin diameter to specific applications, Pall

chromatography resins exhibit the highest

performance characteristics possible while

ensuring reliable, reproducible protein isolation.

Chromatography Products Expand Separation OptionsPall offers chromatography products to facilitate research

needs, scale-up, and polishing. Our chromatography

solutions are available in resin or membrane formats to

support your specific application. Choose from our extensive

portfolio of media including flat sheet membrane, bulk resin,

and media incorporated into specific product housings. Pall

products allow you to tailor your product selection to the

nature of the purification you desire.

BioSepra® resins facilitate high capacity purification

Chromatography continues to be an essential technology

for the purification of biomolecules. Pall’s recent acquisition

of BioSepra products complements our current technology

www.pall.com9

ApplicationsDetergent removal (SDR HyperD Resin)

Protein fractionation (Q, S, DEAE, CM Ceramic HyperD Resins)

IgG purification from various sample types (Protein A Ceramic HyperD F Resin)

IgG purification from cell culture supernatant (MEP HyperCel™ Resin)

Albumin depletion (Blue Trisacryl® M Resin)

Desalting (Trisacryl GF05 M and Ultrogel® AcA 202 Resins)

Tagged biomolecule purification (IMAC HyperCel Resin)

Reference materialSell Sheet, BioSepra Chromatography Media, PN 33400

BioSepra Ion Exchange Resins Exhibit Extremely High Dynamic Binding Capacity

The HyperD line of ion exchange resins shows high dynamicbinding capacity (50-110 mg BSA/mL of resin for resins testedhere). Dynamic binding capacity is measured in a 1 mL packedcolumn by pumping BSA (anion) or lysozyme (cation) at 5 mg/mLin a suitable binding buffer until the column capacity is exceeded. The capacity is then calculated by estimating the volume of proteinrequired to achieve this “breakthrough” and expressed as mg/mLmedia volume. Anion and cation chemistries are available in both 50 µm (HyperD F resins) and 20 µm (HyperD 20 resins) particlesizes for improved resolution.

Flow Rate (mL/min)Media 1 5 10Q Ceramic HyperD® 20 106.0 mg/mL 91.5 mg/mL 82.5 mg/mL

DEAE Ceramic HyperD F 101.5 mg/mL 87.5 mg/mL 77.5 mg/mL

S Ceramic HyperD F 80.5 mg/mL 61.5 mg/mL 53.5 mg/mL

S Ceramic HyperD 20 98.0 mg/mL 89.5 mg/mL 83.5 mg/mL

CM Ceramic HyperD F 108.0 mg/mL 87.5 mg/mL 73.5 mg/mL

Efficient Removal of Detergents from ProteinSolutions Using the BioSepra SDR HyperD Resin

Detergent Protein SolutionsTriton (DBC = 60-80 mg/mL) IgG AT-III Bovine SerumInitial Conc. (ppm) 10,000 10,000 10,000

Final Conc. (ppm) < 10 < 10 340

Removal Efficiency > 99.9% > 99.9% > 95.2%

SDR HyperD resin binds detergents used in viral inactivation processes (e.g., TnBP and Triton* X-100), as well as other common detergents used in protein procedures (e.g., CHAPS,SDS, ASB14). High recovery of proteins (exclusion limit 10 kDa) is obtained. This product exhibits high adsorption capacity for small hydrophobic molecules and is stable in acidic, polar organic and oxidizing solutions.

10

Membranes are recommended in chromatography

applications when there is a need to purify large molecules

or in situations where faster flow is required. Membrane

chromatography is extremely economical because flow rates

are significantly faster than traditional resin chromatography,

decreasing processing time and increasing throughput. Pall’s

Mustang® membranes possess large convective pores and

have dynamic binding capacities that are relatively insensitive

to the effects of high flow rates, even for large molecules

such as plasmids and viruses.

Membrane devices for ionexchange chromatographyspeed processing

Special features

Scaleable

For laboratory-scale applications, Mustang membranes are

available in Acrodisc® units for single samples and AcroPrep™

96-well filter plates for high throughput sample processing.

Devices with Mustang S and Mustang Q membranes can be

scaled up to larger-capacity capsules and cartridges from Pall.

Application-specific Membrane Chemistries

Mustang Q membrane is a strong anion exchanger that

effectively binds plasmid DNA, negatively-charged proteins,

and viral particles. Mustang S membrane is a strong cation

exchanger that effectively binds positively-charged proteins

and viral particles.

Fast Flow Rates for Rapid Separations

Mustang membranes withstand high flow rates to render

faster purification without affecting recovery rates.

www.pall.com11

ApplicationsContaminant removal such as DNA viral particles, host cell proteins, or endotoxin

Isolation via capture and release of plasmid DNA, virus, or target protein from a complex mixture

Protein fractionation or capture

Antibody purification

Reference materialProduct Data, Acrodisc Unit with Mustang S Membrane, PN 33256

Product Data, Acrodisc Unit with Mustang Q Membrane, PN 33255

0

5

10

15

20

25

30

min 0.6 1.2 1.9 2.5 3.1 3.8 4.4 5.0 5.7 6.3Time (min)

Abso

rban

ce28

0nm

(mAU

)

0

5

10

15

20

25

Cond

uctiv

ity(m

s/cm

)

Goat IgG

BSA

AbsorbanceConductivity

Unbound Proteinfrom Goat IgG

0

200

400

600

800

1000

1200

1400

1600

1800

0 5 10 15 20 25

Time (min)

Prot

ein

(mg/

mL)

Elution Peak

54 mg/mL at 0Breakthrough

Acrodisc Unit with Mustang Q Membrane:Resolution with BSA and Goat lgG

Acrodisc Unit with Mustang Q Membrane:Dynamic Binding with BSA

The conditions used to generate data for the resolution graphabove include buffer: 25mM Tris pH 8.0; salt: 1M NaCl in25mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 columnvolume (CV); flow rate: 2.3 mL/min (13 cv/min); sampleloading: 4% of total binding capacity.

A solution of 0.524 mg/mL BSA was pumped through theAcrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1minutes and was calculated as 54 mg/mL using:

flow rate (2.3 mL/min) X initial protein BSA concentration (0.524 mg/mL) X time (8.1 min)

membrane bed volume of Mustang Q membrane in 25 mm Acrodisc unit (0.18 mL)

Particle SizeChromatography Type Product Description (Average) Capacity Primary Applications

Size Exclusion (Gel Filtration)

Separation by Molecule Size Bulk Resin

Ultrogel® AcA Ultrogel AcA are polymeric resins for size exclusion composed 100 µm N/A Fractionation, purification of biomolecules of polyacrylamide and agarose, characterized by narrow particle by size, molecular weight determinationsize distribution.

Trisacryl® GF05 M Trisacryl GF are highly hydrophilic copolymer resins designed 60 µm N/A Lowest exclusion limit for desalting and other for medium pressure gel filtration. small molecule removal

Trisacryl GF2000 LS Trisacryl GF are highly hydrophilic copolymer resins designed 120 µm N/A Purification of macromoleculesfor medium pressure gel filtration.

Ion Exchange

Separation by Charge Bulk Resin

Q Ceramic HyperD® 20 Strong anion exchanger. Ceramic HyperD ion exchangers employ 20 µm > 85 mg/mL (4) Polypeptide and plasmid purificationa high capacity hydrogel polymerized within the large pores of a rigid ceramic bead.

S Ceramic HyperD 20 Strong cation exchanger. Ceramic HyperD ion exchangers employ 20 µm > 85 mg/mL (5) Polypeptide purification a high capacity hydrogel polymerized within the large pores of a rigid ceramic bead.

Q Ceramic HyperD F Strong anion exchanger. Ceramic HyperD ion exchangers employ 50 µm > 85 mg/mL (4) Recombinant proteins, monoclonal antibodies,a high capacity hydrogel polymerized within the large pores of plasmid, vaccine purification, capture stepa rigid ceramic bead.

S Ceramic HyperD F Strong cation exchanger. Ceramic HyperD ion exchangers employ 50 µm > 75 mg/mL (5) Recombinant proteins, monoclonal antibodies,a high capacity hydrogel polymerized within the large pores of vaccine purification, capture stepa rigid ceramic bead.

DEAE Ceramic Weak anion exchanger. Ceramic HyperD ion exchangers employ 50 µm > 85 mg/mL (4) Recombinant proteins, monoclonal antibodies,HyperD F a high capacity hydrogel polymerized within the large pores of plasmid, vaccine purification, capture step

a rigid ceramic bead.

CM Ceramic HyperD F Weak cation exchanger. Ceramic HyperD ion exchangers employ 50 µm > 60 mg/mL (6) Recombinant proteins, monoclonal antibodies,a high capacity hydrogel polymerized within the large pores of vaccine purification, capture stepa rigid ceramic bead.

Q HyperZ® Q HyperZ are specifically designed for high productivity 75 µm > 80 mg/mL (9) Expanded bed and packed bed separationsexpanded bed chromatography and efficient capture of biomolecules directly from crude, unclarified samples in a single pass operation.

CM HyperZ CM HyperZ are specifically designed for high productivity 75 µm ~ 50 mg/mL (10) Expanded bed and packed bed separationsexpanded bed chromatography and efficient capture of biomolecules directly from crude, unclarified samples in a single pass operation.

Membrane Filter Plates and Devices

Mustang® Q Strong anion exchanger. Also available in AcroPrep™ 96 filter plates N/A 50 - 60 mg/mL Protein fractionation350 µL or 1 mL, and Acrodisc® syringe filters.

Mustang S Strong cation exchanger. Also available in AcroPrep 96 filter plates N/A 45 - 50 mg/mL Protein fractionation350 µL or 1 mL, and Acrodisc syringe filters.

Affinity

Separation Using Bulk ResinSpecific Ligands

Blue Trisacryl M Blue Trisacryl M is an affinity chromatographic resin used for the 60 µm HSA: 10 - 15 mg/mL; Albumin depletionpurification of a wide variety of enzymes and proteins such as kinases, BSA: 5 - 7 mg/mL (1)albumin, interferons and some coagulation factors. The basic matrix is Trisacryl GF2000, a macroporous non-ionic resin on which Cibacron* blue is covalently immobilized.

IMAC HyperCel™ IMAC HyperCel uses tridentate IDA (imino-diacetic-acid) as a 90 µm 30 - 60 µmol Tagged biomolecule purificationchelating agent. The ligand is immobilized on the HyperCel Cu++/mL resinbase sorbent, a stable and robust resin.

Protein A Ceramic Protein A Ceramic HyperD F is a high capacity affinity resin 50 µm > 30 mg/mL (2) IgG purification/depletionHyperD F prepared using a rigid proprietary ceramic bead. Recombinant

Protein A is immobilized to a specially formulated hydrogel within the porous ceramic bead.

Heparin HyperD M Heparin HyperD M composite chromatography resin is used to purify 80 µm > 25 mg/mL (3) Purification of coagulation factors,biological molecules that bind to heparin such as coagulation factors, lipoproteins, growth hormones, growth growth factors and lipoproteins. Heparin HyperD M is composed of a factors, nucleic acid binding enzymesporous rigid mineral bead containing heparin bound hydrogel filled pores.

Lysine HyperD Lysine HyperD is used to purify biological molecules that bind to lysine 70 µm N/A Purification of glycoproteinssuch as glycoproteins. Lysine HyperD is comprised of a porous rigid mineral bead containing lysine (L-lysine) bound hydrogel filled pores.

Kits

Enchant™ Albumin For the depletion of albumin from plasma or serum. Includes all N/A > 2 mg albumin Albumin depletion or isolationDepletion Kit buffer and devices needed for 25 purifications. per purification

Enchant Protein A Kit For the purification of IgG. Includes all buffers and devices needed N/A 11 - 19 mg human IgG depletion or purificationfor IgG Purification for 50 purifications. IgG/mL of gel, 6 - 8 mg

mouse IgG/mL of gel

Enchant Protein G Kit For the purification of IgG. Includes all buffers and devices N/A 10 - 15 mg human IgG depletion or purificationfor IgG Purification needed for 10 purifications. IgG/mL of gel

Enchant Multi-Protein For the removal of 99% of albumin and IgG from N/A > 99% removal Albumin and IgG fractionationAffinity Separation Kit serum/plasma samples.

12

Chromatography products selection guide

Particle SizeChromatography Type Product Description (Average) Capacity Primary Applications

Mixed Mode and Hydrophobic Charge Induction (HCIC)

Bulk Resin

MEP HyperCel MEP HyperCel (4-mercapto-ethyl-pyridine) resin is specifically 90 µm > 20 mg/mL (7) Purification/depletion of polyclonal anddesigned for the capture and purification of monoclonal and polyclonal monoclonal antibodies of most speciesantibodies. In contrast to Protein A resins, IgG binding on MEP HyperCel is essentially independent of subclass or species.Weakly binding variants (e.g., murine IgG, rat IgG) are well retained.

SDR HyperD SDR HyperD is a mixed mode of size exclusion, normal phase 80 µm 60 - 80 mg/mL (11) Solvent and detergent removaland reversed phase. It is a unique resin designed to eliminate solvent and detergent while recovering NATIVE protein. SDR HyperD is a composite resin that combines a silica bead moiety filled with long chain aliphatic polymers that are cross-linked to provide a 3D mesh with a low size exclusion limit of 10 kDa which excludes proteins.

Hydroxyapatite

Bulk Resin

HA Ultrogel HA Ultrogel hydroxyapatite resin is composed of cross-linked 120 µm Cytochrome C: Immunoglobulin separation,agarose beads with micro-crystals of hydroxyapatite entrapped > 7 mg/mL (8) glycoproteins, vaccinesin the agarose mesh.

www.pall.com13

Process proteomics centers enhance service

The identification and optimization of protein purification

parameters can be a tedious task. Process proteomics

methodologies can be used to streamline the initial scouting

of protein purification conditions as well as for some of

the optimization steps. Process proteomics is performed

using common chromatographic chemistries (e.g., anion

exchange, cation exchange, IMAC, etc.) on a protein

chip, in the wells of a multi-well filter plate, or using small

columns. Using this approach, multiple binding, washing

and elution conditions can be tested on your sample

simultaneously. Successful scale-up from these small-scale

experiments to traditional column chromatography has

proven to be quite useful.

Pall's Process Proteomics Service Centers assist customers

in selecting and optimizing resins and membranes for the

purification of proteins used in the scale-up and production

of therapeutic proteins and other bioprocess applications.

With access to a large portfolio of both resin and media

technologies, Pall can provide highly integrated solutions

for our customers.

A Unique Combination ofChromatography Modes

(1) capacity determined in PBS buffer using 5 mg/mL

(2) dynamic binding capacity, 10% breakthrough, 100 cm/h, determined using 10 mg/mL hu IgG in PBS, pH 7.4; elution in 0.1 M sodium citrate, pH 2.5; column 4.6 ID x 100 mm

(3) dynamic binding capacity at 600 cm/h, using hu ATIII at 72.5 UI/mL in 20 mM Tris-HCl, 0.3 M NaCl, pH 7.4; elution with 20 mM Tris-HCl, 2 M NaCl, pH 7.4; 10 cm bed height

(4) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6

(5) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL lysosome in 50 mM sodium acetate, pH 4.5

(6) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL hu IgG in 50 mM sodium acetate,100 mM NaCl, pH 7.4

(7) dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in PBS, flow rate: 60 cm/h

(8) capacity for cytochrome c, determined using 5 mg/mL cytochrome c diluted 50/50 in 1 mM phosphate buffer,pH 6.8; at 12.5 cm/h

(9) dynamic binding capacity, 10% breakthrough determined using 5 mg/mL BSA in 50 mM Tris-HCl buffer,pH 8.6; 150 mM NaCl

(10) dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in 50 mM sodium acetate buffer, pH 4.7; 150 mM NaCl

(11) dynamic binding capacity, 10% breakthrough at 300 cm/h, determined using 5 mg/mL Triton* in PBS, pH 7.4

Protein Interaction withCalcium Phosphate

14

As samples get smaller and more numerous, the need for

novel methods to purify proteins and improve assays has

led Pall to develop a broad line of multi-well filter plates

that target specific application challenges. AcroPrep™

and AcroWell™ filter plates feature individually sealed

membranes that eliminate crosstalk and solution weeping.

The proprietary sealing technology allows us to seal virtually

any type of membrane or media configuration into a

device platform to meet ever-changing industry needs.

Special features

Broad Selection to Suit Your Application

The unique nature of protein science results in a variety

of device requirements for each application type. Pall

understands this need and has a full portfolio of 96- and

384-well filter plates that can be optimized for your assay.

Our portfolio includes a selection of single- and multi-layer

membranes, plate colors, well volumes, and outlet tips.

Multi-well Filter Plates Speed Your Rate of Discovery

Pall’s filter plate product line includes two platforms that

address different application needs:

AcroPrep filter plates are engineered with special outlet tipsand splash guards, and can be used for both filtrate- and retentate-based applications. Membranes are individually cut, placed and sealed in the wells using a proprietary sealing process that ensures seal integrity. A distinctive valve technology eliminates sample leaking.

AcroWell filter plates are designed to support retentate and hybridization-based binding applications. These plates are constructed of two membrane layers; the bottom layer protects the upstream functional membrane and acts as a barrier to passive flow.

Using a plate optimized for your application will reduce

sample loss, make automation easy, and add consistency

and reliability throughout your entire process.

www.pall.com15

Automation Compliant

Pall’s plates are designed in accordance with the standards

of the ANSI/SBS X-2004. Rigid construction enables the

plates to be easily maneuvered by robotic instrumentation

and assures that the plates will seat properly on vacuum

manifolds, wash stations, hotel carousels and deck

platforms. Plates are compatible with industry leading

workstations including: Tecan, Qiagen Inc., Tomtec,

PerkinElmer Life and Analytical Sciences, Beckman Coulter

Inc., Caliper Life Sciences, Inc., Waters, Proteodyne, and

Hamilton Company.

Mass Spec Friendly, Chemically Resistant, and Low Binding

The polypropylene housing assembly has been tested to

ensure that the materials of construction do not contribute

to ion suppression/enhancement. In addition, the housing

materials and media have been optimized and tested to

reduce extractables, ensuring that unwanted materials are

not introduced to your sample. The housing is compatible

with a broad range of aqueous and organic liquids, and is

noted for its low biomolecule binding that minimizes non-

specific adsorption of samples to the plates.

No Crosstalk

Specially engineered fluid directors and outlet tips on the

bottom of the AcroPrep plate are designed to reduce the

potential for downstream crosstalk. Elimination of crosstalk

upstream is assured through individually sealing a membrane

in each well using Pall’s proprietary sealing technology. Pall

understands the critical nature of each sample, and we

know that seal failure will cause sample loss. To ensure

integral sealing of each well, we test each lot of product for

seal integrity prior to release. You can be assured that

AcroPrep and AcroWell filter plates will provide a robust

platform that will eliminate concerns of sample loss and

cross contamination.

16

Efficient Optimization of Protein PurificationConditions: AcroPrep™ 96 Filter Plates as “Mini-chromatography” Columns

Although size separation applications can be done effectively

using ultrafiltration, a more specific affinity Immobilized Metal

Affinity Chromatography (IMAC) system is typically needed

to purify tagged biomolecules from crude lysates. Pall

has demonstrated the use of our multi-well filter plates to

perform high throughput IMAC. The low protein binding

and low weeping properties of the AcroPrep 96 filter plate

with low protein binding membrane are ideal for convenient

incubation of the sample directly in wells. The biomolecule-

friendly AcroPrep 96 filter plate allows the researcher to

rapidly and reliably screen numerous samples under a

variety of conditions to determine protein purification

parameters. A single filter plate can be matrixed to:

Screen for metal ions for both custom and pre-charged resins.

Optimize elution conditions.

Optimize resin-to-load ratio.

Aliquots of Ni-NTA resin (Qiagen) were mixed with E. coli inclusionbody lysate containing a His-tagged TEV protease construct(load). The slurry was either incubated in a microfuge tube andthen transferred to a filter plate (left panel) or incubated directly in a well of an AcroPrep 96 filter plate with 0.2 µm Bio-Inert®

membrane (right panel). After washing, the samples were seriallyeluted with either 3 X 200 µL (left panel) or 3 X 50 µL (right panel)of elution buffer. The load (L), flow through (FT), wash (W1 andW2), and elution (E1, E2, and E3) were analyzed by SDS-PAGE.The incubation of sample/resin slurry directly in the wells of thefilter plate gave similar recoveries to those incubated in microfugetubes, allowing the simplification of sample handling. Based onthis observation, the on-plate incubation procedure was used for the subsequent experiments.

The AcroPrep 96 filter plate shows consistent well-to-well

performance, giving protein biochemists an edge in the

development of protein purification protocols.

M L FT W1 W2 E1 E2 E3

Incubation in Centrifuge Tubes

Incubation in AcroPrep 96 Plates

M FT E1 E2 E3

www.pall.com17

Efficient Desalting and High Protein Recovery Using AcroPrep 96 Filter Plates

The efficiency of AcroPrep 96 ultrafiltration filter plates to

remove salts and other small molecules does not diminish

sample recovery. Pall has demonstrated that when using

an AcroPrep 96 filter plate with 10K Omega™ membrane,

the recovery of ovalbumin proteins (45 kDa) at two different

concentrations (0.1 and 1.0 mg/mL) was greater than 90%.

Salt removal efficiencies were also greater than 95%. Using

BSA (66 kDa) and a 30K Omega membrane, similar protein

recoveries and desalting efficiencies were observed.

300 µL of indicated protein solutions was added to wells of 10K or 30K plates. Each test plate was matched to a receiver plate and the assembly spun at 2,000 x g for 40 min. Following centrifugation,retained proteins were collected by adding 300 µL of buffer to eachassay well, then allowing the plate to stand at room temperature for 5 min. before pipetting up and down 10 times to remove sample to fresh tubes. Protein concentration was determined using UVspectrophotometric analysis (n = 3). Percentage of salt removal was determined using a conductivity meter. A representative experiment is shown.

AcroPrep 96 Filter Plate with 10K Omega Membrane

AcroPrep 96 Filter Plate with 30K Omega Membrane

99.1 9497.3 93

0

1020

30

40

5060

70

8090

100

% Salt Removed % Protein Recovered

Ovalbumin (45kDa) 0.1 mg/mL,500 mM NaCl

Ovalbumin (45kDa)1.0 mg/mL200 mM NaCl

99.2 9498.1 95

0

10

20

30

40

50

60

70

80

90

100

% Salt Removed % Protein Recovered

BSA (66kDa) 0.1 mg/mL,500 mM NaCl

BSA (66kDa) 1.0 mg/mL,200 mM NaCl

ApplicationsBead-based applications Lysate clarification

Protein concentration Protein fractionation

Protein purification Gross fractionation

Protein desalting Size exclusion separations

Reference materialSell Sheet, AcroPrep and AcroWell Multi-well, Membrane-bottom Plates, PN 33287

Product Data, AcroPrep Membrane-bottom Plates, PN 33296

Product Data, AcroWell 96 Membrane-bottom Plates, PN 33306

Protocol, Desalting/Buffer Exchange for Biomolecules Using AcroPrep 96 Ultrafiltration Filter Plates, PN 33309

Protocol, Lysate Clearance for Prokaryotic DNA Isolation Using the AcroPrep 96 Filter Plate, PN 33308

Protocol, Automated Purification of Combinatorial Libraries Using AcroPrep 96 Filter Plate with GHP Membrane, PN 33245

Protocol, Biomolecule Binding and Blocking Procedures for AcroWell 96 Filter Plates with BioTrace™ NT and BioTrace PVDF Membranes, PN 33189

Protocol, Using the AcroWell 96 Filter Plate for Receptor/Ligand Binding, PN 33179

Technical Report, IMAC Purification of Polyhistidine-tagged Protein Using the AcroPrep 96 Filter Plate, PN 33354

Technical Report, Automated Plate ELISA and Dot-Blot Assays Using AcroWell 96 Filter Plates and a Robotic Workstation with Integrated Plate Reader, PN 33293

Technical Report, Development of a Fluorescent Ligand-Binding Assay Using the AcroWell Filter Plate, PN 33220

Technical Report, The AcroWell 96 Filter Plate: Low Fluore-scence Background Using the DELFIA* System, PN 33137

Technical Report, The AcroWell Filter Plate Minimizes Crosstalk, see www.pall.com/proteomics

18

Pall’s ultrafiltration centrifugal devices simplify many common

protein handling procedures. These devices provide efficient

concentration and salt removal of samples from 50 µL to

60 mL in just minutes. Choose from membranes that have

been developed to assure low nonspecific biomolecule

binding and provide consistent, high recovery of target

molecules. Ultrafiltration reduces the amount of handling

that can cause damage to samples, leaving concentrated

samples ready for direct incorporation into downstream

applications at critical stages in the discovery process.

Centrifugal Devices Facilitate Pure,Concentrated Product with High Recoveries

Pall’s microfiltration centrifugal devices are used in protein

separation and small-scale general filtration procedures.

These devices can be used in combination with

chromatography resins to create a fast, efficient

method for purifying proteins of interest.

Special features

Rapid Processing

Achieve high recoveries in as little as five to ten minutes.

High Performance Membranes

Omega™ polyethersulfone ultrafiltration membrane provides

higher flow rates and is lower protein binding than competitive

membranes. This results in lower processing time and the

highest possible recoveries.

Low Protein Binding

Devices are constructed of low-binding materials

to maximize sample recovery.

Variety of MWCO’s and Pore Sizes

Available with ultrafiltration membranes for rapid concentrating

and/or desalting of proteins. Also available with low-binding

microfiltration membranes for particulate removal or

chromatography separations.

Easy to Use

Once you have identified the right MWCO or pore size ranging

from 1 kD to 0.45 µm, the devices are color-coded for easy

visual identification.

Ideal for Fast Batch Mode Chromatography Applications

Nanosep® centrifugal devices with microfiltration membrane

serve as a perfect housing for chromatography resin. The spin

device can be filled with the resin of choice to perform the

desired protein purification application.

www.pall.com19

Match device size to sample volume

Pall’s centrifugal devices are available in a range of

sizes to accommodate your specific sample volumes.

AcroPrep™ filter plates can be used with smaller sample

volumes in similar applications.

Device Sample VolumeAcroPrep 384 filter plate < 100 µL

AcroPrep 96 filter plate, 350 µL < 350 µL

AcroPrep 96 filter plate, 1 mL < 1 mL

Nanosep device < 0.5 mL

Microsep™ device 0.5 - 3.5 mL

Macrosep® device 3 - 15 mL

Jumbosep™ device 15 - 60 mL

Easy-to-assemble stirred cell systems process 2 to 150 mL

Pall’s Stirred Cell Systems

provide a versatile format that

can be disposed of when working

with biologically hazardous or

radioactive materials, or cleaned

and reused up to 20 times.

These devices are 100% integrity tested to ensure the

membrane and cell reservoir are integral. Ultrasonically

sealed membranes eliminate the need for O-rings that can

leak. The devices are easy to assemble, use and clean up,

and feature as much as 50% more effective filtration area

than conventional stirred cells of the same volume.

ApplicationsConcentrate, purify and desalt peptides and proteins

Separate proteins from acrylamide gels

Prepare samples for HPLC analysis

Fractionate proteins

Reference materialSell Sheet, Centrifugal Devices, PN 33327

Product Data, Centrifugal Devices for Ultrafiltration and Microfiltration, PN 32984

Product Data, Stirred Cell Systems and Ultrafiltration Membrane Disc Filters, PN 32985

Protocol, Desalting/Buffer Exchange for Biomolecules Using AcroPrep 96 Ultrafiltration Filter Plates, PN 33309

Protocols, Nanosep Centrifugal Devices, PN 32989

Technical Report, Fast and Efficient Elution of Proteins from Polyacrylamide Gels Using Nanosep Centrifugal Devices, see www.pall.com/proteomics

Technical Report, Purification and Handling of DNA Fragments, see www.pall.com/proteomics

Technical Report, Nanosep Centrifugal Ultrafiltration Devices and PCR: Before and After, see www.pall.com/proteomics

Technical Report, Single-tube DNA Purification and Cloning Using Ultrafiltration Devices, see www.pall.com/proteomics

Nanosep Devices Exhibit Fast Spin Times and High Recoveries

Samples of 0.5 mL of a 1.0 mg/mL solution were centrifuged at 14,000 x g andconcentrated to a volume of 10 to60 µL using Nanosep centrifugaldevices with Omega membrane.

MWCO 3K 10K 30K 100K 300KSolute Solute MW (Kd) Spin Time (min.) 15 10 8 5 3Vitamin B12 1,335 % Recovery 7 - - - -

Aprotinin 6,200 % Recovery 99 51 11 - -

Cytochrome C 12,400 % Recovery 100 89 77 1.8 -

Chymotrypsinogen A 25,000 % Recovery - 97 94 2.1 -

Ovalbumin 45,000 % Recovery - 97 92 3 -

BSA 67,000 % Recovery - - 100 26 1.5

Phosphorylase B 97,400 % Recovery - - 95 91 1

IgG 156,000 % Recovery - - - 97 1.5

Thyroglobulin (1 mg/mL) 677,000 % Recovery - - - 100 91

20

The first step in isolating new drug targets is critical, and

reliable purification with minimal loss is key. Biospecific

affinity ligand technologies such as Pall’s Enchant Protein A

or G antibody purification/depletion kits are widely used in

immunoglobulin purification for research and therapeutic

applications. Enchant Protein Purification Kits rapidly deplete

unwanted abundant proteins and unmask low abundant

biomarkers from human and animal-derived serum and

plasma samples. Alternately, these same kits can be used

to collect the abundant protein fraction for further analysis.

Convenient kit formats eliminate the need to handle messy

slurries or deal with column packing. These all-in-one kits

include the protocol, purification columns and buffers, and

offer one of the lowest costs per sample available.

Enchant™ Protein Purification Kits Expedite Proteomic Sample Prep

Special features

Enchant Albumin Depletion or Purification KitEffectively process up to 100 µL of serum or plasma in 5 simple steps.

Remove > 2 mg of albumin per column.

Remove albumin from multiple species including human, rat, goat, calf, and bovine.

Albumin can be discarded or recovered for further analysis.

Enchant IgG Depletion or Purification Kits High binding. Bind between 11-19 mg of human IgG/mL of gel (typical binding capacity).

Purify a variety of IgG molecules from a broad range of species including human, horse, mouse, rat, cow, goat, etc.

Each column can be regenerated and used for ten purifications.

Convenient kits contain all components necessary to affinity purify or deplete IgG with either Protein A or Protein G affinity resin from ascites fluid, serum or plasma.

IgG can be discarded or recovered for further analysis.

Enchant Multi-Protein Affinity Separation KitAchieve 99% removal of albumin and IgG from human serum or plasma samples.

High specificity. Will not remove low abundant, low molecular weight biomarkers.

No loss of protein on the fractionation columns.

Can process up to 50 µL human serum or plasma in just 15 minutes.

Albumin and IgG fractions can be further fractionated and analyzed.

Reference materialSell Sheet: Enchant Albumin Depletion Kit, PN 33355

Sell Sheet: Enchant IgG Purification Kits, PN 33356

Product Data, Enchant Life Science Kits Albumin Depletion, see www.pall.com/proteomics

Product Data, Enchant Life Science Kits IgG Purification, see www.pall.com/proteomics

www.pall.com21

Effective Depletion of Human Serum Albumin (HSA)and IgG from Plasma and Serum Samples

Depletion of both IgG and HSA using Enchant Abundant ProteinDepletion kits. One mL of human plasma was processed using theEnchant Protein A IgG purification kit. Then, 30 µL of the columnflow through was treated with the Enchant albumin depletion kit.Samples were loaded onto 4-12% SDS-PAGE gels and resolved in MOPS/SDS running buffer under non-reducing conditions.Proteins were visualized with colloidal Coomassie* blue. Left panelshows results for human plasma; right panel shows results forhuman serum. Lane 1 in both panels shows starting material, 1 µL. Lane 2 shows IgG removal from the Protein A column flowthrough. Lane 3 shows subsequent removal of albumin.

1 2 3 1 2 3

IgG

HSA

Visualize Low Abundant Proteins with EffectiveAlbumin Depletion

Two dimensional gel electrophoresis (2DGE) analysis of humanplasma following treatment using the Enchant Albumin Depletion kit.Briefly, 20 µL of human plasma was diluted with 30 µL of bindingbuffer and added to the Enchant albumin depletion column.Sample was incubated for 10 minutes at room temperature thenspun at 12,000 x g for one minute. Filtrate was recovered, addedback to resin, incubated for 2 minutes and spun again. Therecovered filtrate and starting material were analyzed by 2DGE byfocusing in the first dimension on a pH 4-7 IPG strip then resolvingthe second dimension on an 8-16% tris-glycine gel under reducingconditions. Proteins were visualized with colloidal Coomassie blue.

Versatile Purification and Depletion of IgG and HSA from Multiple Species: Protein A Kit

Purification of different species’ IgG’s using Enchant Protein A IgGPurification kit. One mL of plasma (human, rat) or serum (rabbit,mouse) was processed according to insert instructions. The plasma or serum starting material (1 µL – Lanes 1, 3, 5, 7) and one eluatefrom each species with an A280 of approximately 1.0 (10 µL – Lanes2, 4, 6, 8) were loaded onto 4-12% SDS-PAGE gels and resolvedunder non-reducing conditions in MOPS/SDS running buffer. Proteinswere visualized with colloidal Coomassie blue staining. Efficientdepletion occurred with each species processed.

1 2 3 4 5 6 7 8

IgG

IgG HC

IgG LC

Human Rabbit Mouse Rat

Versatile Purification and Depletion of IgG and HSA from Multiple Species: Protein G Kit

Purification of different species’ IgG’s using Enchant Protein G IgGPurification kit. One mL of plasma (human, rat) or serum (rabbit,mouse) was processed according to insert instructions. The plasmaor serum starting material (1 µL – Lanes 1, 3, 5, 7) and one eluatefrom each species with an A280 of approximately 1.0 (10 µL – Lanes2, 4, 6, 8) were loaded onto 4-12% SDS-PAGE gels and resolvedunder non-reducing conditions in MOPS/SDS running buffer. Proteinswere visualized with colloidal Coomassie blue staining. Efficientdepletion occurred with each species processed.

IgG

IgG HC

IgG LC

Non-depleted

Depleted

Plasma Serum

1 2 3 4 5 6 7 8

Human Rabbit Mouse Rat

22

Tangential Flow Filtration (TFF) is a rapid and efficient

method for separating and purifying biomolecules. Pall’s

TFF products combine low protein binding ultrafiltration or

microfiltration membranes with optimized flow path design

to quickly concentrate samples while achieving high

concentration factors for sample volumes from 10 mL

to thousands of liters. Systems are designed for easy set

up and use. By incorporating the same path length and

materials of construction throughout our TFF product line,

conditions established during pilot-scale trials can easily be

applied to process-scale applications.

Increase Productivity Using Tangential Flow Filtration

Special features

Easy Set Up and Use

Simply connect the TFF device to a pump and pressure

gauge(s), add sample, and process.

High Concentration Factors

Low hold-up volumes allow high concentration factors

to be achieved from small starting volumes.

Fast and Efficient Processing

Higher concentrations can be achieved in less time than with

centrifugal devices or stirred cells. Sample concentration and

diafiltration can be achieved on the same system, saving

time and avoiding product loss.

Scale Up or Down

Identical fluid path lengths and materials of

construction allow precise linear scale-up to

larger systems. The membrane area of a

smaller device can be increased simply by

connecting multiple devices or adding cassettes.

Assuring predictable performance saves time when

scaling a process from pilot to production.

Economical

TFF devices and cassettes can be cleaned and reused, or

disposed of after a single use. A simple integrity test can be

performed to confirm that membrane and seals are intact.

www.pall.com23

General Product Selection Based on Starting Sample Volume

Recommended Typical Filtrate Retentate Flow Rate/ Minimum

TFF Capsule Membrane Area/ Flow Rate** at Capsule or Cassette Starting Sample Concentratedor Cassette* Capsule or Cassette 50 LMH 20 °C for Screen Channel Volume Range Volume***

LAB SCALE/SCALE-UPMinimate™ 50 cm2 (0.05 ft2) 4 mL/min 30 - 80 mL/min 25 - 1000 mL < 10 mL

LV Centramate™ 0.01 m2 (0.1 ft2) 8 mL/min 60 - 80 mL/min 40 - 2000 mL 10 mL

LV Centramate 0.02 m2 (0.2 ft2) 15 mL/min 120 - 160 mL/min 60 - 4000 mL 15 mL

PROCESS DEVELOPMENT AND SMALL-SCALE PRODUCTIONUltrasette™ 0.084 m2 (0.9 ft2) 4 L/hr 1200 - 1500 mL/min 0.2 - 5 L 100 mL

Centramate 0.093 m2 (1.0 ft2) 4.6 L/hr 600 - 800 mL/min 0.2 - 25 L 100 mL

* Data is per unit or cassette. Centramate holder can hold fivecassettes. Other column data can be calculated by multiplying table values by the number of cassettes installed in the holder.

** Typical filtrate flow rate is based on an average filtrate flow rate of 50 LMH and a process time of about four hours. Actual valuemay be higher or lower depending on the MWCO of membrane,

sample composition and viscosity, operating conditions, i.e.,transmembrane pressure, cross flow rate, temperature, etc.

*** Minimum concentrated volume depends on system hold-upvolume, reservoir design and pump type and speed. Smallervolumes can be achieved by minimizing tubing lengths and use of properly sized components, tubing, fittings, etc.

Optimize performance byselecting the proper device

Choosing the appropriate cassette or device size depends

on the total sample volume, the required process time, and

the desired final sample volume. Performance parameters

for Pall’s laboratory TFF devices are presented below.

24

Minimate™ TFF system streamlines lab-scale concentration, desalting, andbuffer exchange processes

The Minimate TFF system efficiently concentrates samples

from up to one liter to as little as 5 mL, enabling high

concentration factors. Subsequent desalting or buffer

exchange steps can be run on the same system with

minimal user intervention. The system works with Pall’s

Minimate TFF capsule, a disposable device designed to

accelerate and simplify scale-up applications.

www.pall.com25

Gain Precise Control of Protein Concentration with the Minimate TFF System and a LiquidChromatography System

-50

0

50

100

150

200

250

5 10 15 20 25 30 35 40

Time (min)

Abso

rban

ce(m

AU)

0

5

10

15

20

25

Cond

uctiv

ity(m

S/cm

)

Conductivity

A280

A600

The concentration of a 1 mg/mL BSA solution was accomplishedby leaving the diafiltration feed line open to air. The run, using aMinimate 10K capsule, was processed at 25 mL/min recirculationrate. Salt, protein (A280) and turbidity (A600) were monitored using in-line sensors.

Facilitate Sequential Buffer Exchange andConcentration with the Minimate Capsule and a Liquid Chromatography System

-100

100

300

500

700

900

1100

80 100 120 140 160 180 200 220 240 260

Time (min)

Abso

rban

ce(m

AU)

0

10

20

30

40

50

60

70

80

90

100

Cond

uctiv

ity(m

S/cm

)

Conductivity

A280

A600

Sequential diafiltration followed by concentration was documentedfor a single run using a 1 mg/mL BSA solution in 1X PBS, 1MNaCl starting solution. The run using a Minimate 10K capsule wasprocessed at 25 mL/min recirculation rate with the buffer exchangefrom high salt to low salt. The diafiltration buffer feed line was thenopened to air, allowing the concentration of the sample. Salt,protein (A280) and turbidity (A600) were monitored using in-line sensors.

ApplicationsConcentration and desalting proteins and peptides

Protein fractionation

Sample preparation prior to or post chromatography

Reference materialBrochure, Improve Biopurification Processes, PN 33377-BIO

Data Sheet, Minimate Tangential Flow Filtration System and Minimate TFF Capsule, PN 33366

Technical Report, Increased Productivity Using Minimate Capsules to Replace Stirred Cell Systems, PN 33342

Technical Report, The Partnership of the Minimate TFF Capsulewith Liquid Chromatography Systems Facilitates Lab-scale Purifications and Process Development Through In-line Monitoring, PN 33339

Technical Report, Desalting and Buffer Exchange by Dialysis, Gel Filtration or Diafiltration, PN 33290

Technical Report, Diafiltration: A Fast, Efficient Method for Desalting or Buffer Exchange of Biological Samples, PN 33289

Technical Report, Introduction to Tangential Flow Filtration for Laboratory and Process Development Applications, PN 33213

Minimate TFF Capsule Frequently Asked Questions, see www.pall.com/proteomics

26

Although a basic filtration concept, the clarification and

prefiltration of samples remains an important function within

proteomics. When filtration is used as a prefilter, matching

the proper filter media and device to the application is

critical. Here, larger pore size filter materials are used to filter

solutions prior to more detailed analysis. When selecting the

best product for your application, numerous factors need to

be considered. Sample viscosity, sample volume and

sample recovery are just some of the aspects that will drive

the selection of the optimal device.

Simplify Prefiltration and Clarification Procedures

Device

VacuCap® PFBottle-top Filters

Serum Acrodisc®Syringe Filters

Acrodisc PSFSyringe Filters

Acrodisc PFSyringe Filters

AcroPrepTM 96Filter Plates

46284638

4524 4525

AP-4523

46584187

5053 5041 5046

Sample Viscosity

Recommended Part Numbers

Sam

ple

Volu

me

Pall offers a number of media and device options for fast,

effective filtration with minimal sample hold-up for both

single sample and high throughput processing. From

sample volumes of a few microliters to multiple liters, Pall

can supply the best product solution for your application.

www.pall.com27

Rapid, Effective Clarification of Plasmid Purification Lysates Increases Recoveries

The use of filtration for the clarification of plasmid purification

lysates enables a rapid and effective alternative to centrifugal

sedimentation. The AcroPrep 96 filter plate with an integral

prefilter configuration improves the time and effectiveness

of filtration by allowing the prefiltration of viscous samples.

The use of an integral prefilter shortens filtration times,

allows greater flow rates, and results in high recoveries

equal to sedimentation.

Filtration times were measured for 100 µL and 200 µL lysatesamples. Average flow rates were calculated for the AcroPrep 96filter plate with Bio-Inert® membrane (PN 5042), AcroPrep 96 filterplate with Glass Fiber prefilter over Bio-Inert membrane (PN 5046),and a Competitor hydrophilic plate containing a floating prefilter(COMP). Error bars indicate standard error (n=8).

For the complete protocol see, “Lysate Clearance for

Prokaryotic DNA Isolation Using the AcroPrep 96 Filter

Plate,” PN 33308.

12

10

8

6

4

2

0

Flow

Rate

(mL/

sec)

100 µL 200 µLVolume Filtered

PN 5042PN 5046COMP

Built-in Prefilter Enhances Throughput of Viscous,Particulate-laden or Proteinaceous Solutions

The more particulate-laden and/or the higher the protein

concentration in a solution, the more difficult it is to filter.

These types of liquids may clog filters prematurely. The

integration of a prefiltration media dramatically increases both

the throughput and flow rates of proteinaceous solutions.

Acrodisc and Acrodisc PF syringe filters with 0.2 µm Supor®

membrane were challenged with bovine serum or a bacterialculture (107 cfu/mL) at a constant pressure of 1.4 bar (140 kPa, 20 psi). The use of a prefilter (PF) device significantly increased throughput and flow rate.

100%

Calf

Seru

m(m

L)

Time (sec)

0

2

4

6

8

10

0 5 10 15 20

B.di

min

uta

(mL)

Time (sec)

0

10

20

30

40

50

0 5 10 15 20

0.2 µm PF

Reference materialBrochure, Improve Biopurification Processes, PN 33377-BIO

Product Data, Sterile Acrodisc Syringe Filters, PN 33175

Product Data, VacuCap and VacuCap PF Vacuum Filtration Devices, PN 32914

Product Data, AcroPrep Membrane-bottom Filter Plates, PN 33296

Technical Report, Syringe Filter Efficiency and Effect of Filtration on HPLC Column Life, PN 33312

28

For protein characterization, the use of membrane tech-

nology is ideal for obtaining detailed structural information.

Pall offers an impressive range of membranes compatible

with all common protein detection procedures. Our

membranes feature superior binding capacities and

extremely low background to facilitate the transfer and

retention of rare and low-abundant proteins for detection

and further characterization.

Pall’s strict quality manufacturing specifications ensure

consistent, precise membrane performance from

lot to lot and blot to blot. Our membranes set industry

standards for reproducible results, durability, and high

signal-to-noise ratios. Choose from a range of commonly

used formats including rolls, discs, sheets, or custom cuts.

PVDF Membranes

FluoroTrans®, FluoroTrans W, and BioTrace™ PVDF

membranes are hydrophobic in nature and strongly bind

proteins of interest. FluoroTrans membrane is recommended

for protein sequencing and is compatible with all reagents

involved in the Edman reaction. FluoroTrans W and BioTrace

PVDF membranes exhibit low background and high tensile

strength, and are the best membranes available for detection

of protein after Western transfer. All FluoroTrans membranes

exhibit exceptionally low burn through.

Nitrocellulose Membranes

BioTrace NT pure nitrocellulose membrane can be used

for Western transfers and ELISpot assays. The membrane

offers high protein binding and is compatible with a wide

range of protein stains and immunodetection techniques.

Activated Membranes

Pall offers two activated membranes for covalent protein bind-

ing: UltraBind™ modified polyethersulfone has a high ratio of

covalent to non-covalent protein binding; Immunodyne® ABC

membrane offers the sharp spot geometry of nylon with a

proprietary covalent attachment chemistry.

Obtain High Sensitivity for Protein Detection

Gain sensitive, detailed structural information

Rabbit reticulocyte lysate (GE Healthcare) was loaded in lanes of polyacrylamide gels at full strength, 1/3 and 1/10 dilutions. After electrophoresis, proteins were transferred to membranes.Membranes were stained with 0.1% Amido Black, 45% methanol,2% acetic acid for 4 minutes and were then destained for 5 minutes with two changes of 90% methanol, 2% acetic acid. Stained membranes were rinsed in water and air dried.

FluoroTrans Membrane has Excellent Sensitivity,Signal, and Extremely Low Background in Western Transfers

FluoroTransmembrane

FluoroTrans Wmembrane

Competitor PVDF membrane

Avoid membrane damage, make filter handling easy

Pall’s forceps feature flat, smooth tips that gently handle

membrane filters. Polypropylene finger grips provide a

comfortable and secure hold. Choose traditional black

or multi-colored finger grips for forceps that are easy

to identify, track, and see on the lab bench.

www.pall.com29

High Sensitivity Double Antibody Assay for HSR Using Vivid Microarray Slides

1st antibody: Mouse anti-human HSA. 2nd antibody: Goat anti-mouse IgGconjugated to APC. Eight dilutions of HSAwere printed onto a VividGene Array slide using a Genomic Solutions G3 robot; 4x4 blocks; 4 blocks per dilution.Spots are visible at the lowest level printed (10 pg).

pg/spot

1,250

625

312

156

78

39

20

10

Direct fluorescent detection with Vivid™ microarray slides

Vivid Microarray Slides exhibit good signal to noise and

dose response along with excellent sensitivities when used

for double antibody type assays. Easy protocols, simple

immobilization steps, and automation-friendly design make

Vivid slides the ideal choice for consistent, detectable results.

ApplicationsWestern transfers

N-terminal protein sequencing

ELISA

Affinity separation

ELISpot Assays

Reference materialProduct Data, Membranes for Transfer and Immobilization, PN 33082

Product Data, AcroWell 96 Membrane-bottom Filter Plates, PN 33306

Protocol, Double Antibody Nanoimmunoassay with Direct Fluorescent Detection, PN 33273

Protocol, Biomolecule Binding and Blocking Procedures for AcroWell 96 Filter Plates with BioTrace NT and BioTrace PVDF Membranes, PN 33189

Protocol, Transfer and Detection Procedures for Pall Life Sciences Membranes, PN 33167

Technical Report, Automated Plate ELISA and Dot-Blot Assays Using AcroWell 96 Filter Plates and a Robotic Workstation with Integrated Plate Reader, PN 33293

AcroWell™ 96 filter plates expand detection potential

For protein detection in a multi-well format, AcroWell 96 filter

plates are excellent for parallel or automated applications.

Choose plates with BioTrace NT (nitrocellulose) or PVDF

membrane. See pages 14 – 17 for more information on

filter plates.

30

Ordering InformationChromatography resins(lab scale volumes)

Blue Trisacryl® M Affinity Chromatography ResinPart Number Description Packaging

25896-051 Blue Trisacryl M 5 mL




DEAE and SP Spherodex® LS Silica/DextranComposite Ion Exchange ResinsPart Number Description Packaging

26455-023 DEAE Spherodex LS 100 g

20080-024 SP Spherodex LS 100 mL

HA Ultrogel® HydroxyapatiteChromatography ResinPart Number Description Packaging

24775-075 HA Ultrogel Hydroxyapatite 5 mL




Heparin HyperD® M Affinity Chromatography ResinPart Number Description Packaging

20029-062 Heparin HyperD M 5 mL




IMAC HyperCel™ Chromatography ResinPart Number Description Packaging

20093-069 IMAC HyperCel 5 mL



Lysine HyperD Chromatography ResinPart Number Description Packaging

20059-058 Lysine HyperD 5 mL




MEP HyperCel Hydrophobic Charge Induction Chromatography (HCIC) ResinPart Number Description Packaging

12035-069 MEP HyperCel 5 mL




Methyl Ceramic HyperD F Chromatography ResinPart Number Description Packaging

20051-033 Methyl Ceramic HyperD F 25 mL



Protein A Ceramic HyperD F AffinityChromatography ResinPart Number Description Packaging

20078-036 Protein A Ceramic HyperD F 5 mL




SDR HyperD Solvent-Detergent Removal Chromatography ResinPart Number Description Packaging

20033-065 SDR HyperD 5 mL



20033-015 SDR HyperD 1000 mL

Q and CM HyperZ® Ion Exchange ResinsPart Number Description Packaging

21012-010 Q HyperZ 50 g

21012-020 Q HyperZ 250 g

21012-030 Q HyperZ 1 kg

21011-010 CM HyperZ 50 g

21011-020 CM HyperZ 250 g

21011-030 CM HyperZ 1 kg

Q, S, DEAE, CM Ceramic HyperD,CM Trisacryl M Ion Exchange ResinsPart Number Description Packaging

20040-051 Q Ceramic HyperD 20 5 mL





20038-055 S Ceramic HyperD 20 5 mL





20066-098 Q Ceramic HyperD F 5 mL




20062-089 S Ceramic HyperD F 5 mL




20067-070 DEAE Ceramic HyperD F 5 mL




20050-084 CM Ceramic HyperD F 5 mL




26708-016 CM Trisacryl M 300 mL

Trisacryl GF Size Exclusion Chromatography ResinsPart Number Description Packaging

25914-060 Trisacryl GF05 M 100 mL


25916-040 Trisacryl GF05 LS 100 mL

25916-016 Trisacryl GF05 LS 1000 mL


26064-022 Trisacryl GF2000 M 1000 mL

26065-045 Trisacryl GF 2000 LS 100 mL

26065-011 Trisacryl GF 2000 LS 1000 mL

Ultrogel AcA Size Exclusion Chromatography ResinsPart Number Description Packaging

23013-025 Ultrogel AcA 22 100 mL

23013-014 Ultrogel AcA 22 1000 mL


23015-019 Ultrogel AcA 34 1000 mL


23022-015 Ultrogel AcA 44 1000 mL


23019-011 Ultrogel AcA 54 1000 mL

24892-022 Ultrogel AcA 202 100 mL

24892-010 Ultrogel AcA 202 1000 mL

Ion exchange chromatography devices

AcroPrep™ 96 Filter Plates, 350 µL WellPart Number Description Packaging

5047 Mustang® Q membrane, natural 10/pkg

5048 Mustang S membrane, natural 10/pkg

AcroPrep 96 Filter Plates, 1 mL WellPart Number Description Packaging

5062 Mustang Q membrane, natural 5/pkg

5063 Mustang S membrane, natural 5/pkg

Acrodisc® Units, Mustang MembranesPart Number Description Packaging

MSTG25Q6 Acrodisc unit with Mustang Q 10/pkgmembrane, 0.8 µm, 25 mm,(blister non-sterile packs)

MSTG25S6 Acrodisc unit with Mustang S 10/pkgmembrane, 0.8 µm, 25 mm,(blister non-sterile packs)

www.pall.com31

Multi-well filter plates

AcroPrep™ 96 Filter Plates, 350 µL WellPart Number Description Packaging

5033 3K Omega™ membrane, natural 10/pkg

5034 10K Omega membrane, natural 10/pkg



5045 0.2 µm GHP membrane, natural 10/pkg


5043 0.45 µm GHP membrane, white 10/pkg

5044 0.45 µm GHP membrane, black 10/pkg

5037 0.2 µm PTFE membrane, natural † 10/pkg

5038 0.45 µm PTFE membrane, 10/pkgnatural †

5042 0.2 µm Bio-Inert® membrane, 10/pkgnatural

5046 3.0 µm glass fiber media/0.2 µm 10/pkgBio-Inert membrane, natural

5031 1.0 µm glass fiber, natural †† 10/pkg

5032 1.0 µm glass fiber, white †† 10/pkg

5029 0.45 µm Supor® membrane, 10/pkgnatural

5039 1.2 µm Supor membrane, natural 10/pkg

5041 Prefilter material/1.2 µm 10/pkgSupor membrane, natural

5047 Mustang Q ion exchange 10/pkgmembrane, natural

5048 Mustang S ion exchange 10/pkgmembrane, natural

5049 54 µm screen, natural 10/pkg

AcroPrep 96 Filter Plates, 1 mL Well Part Number Description Packaging



5055 0.2 µm PTFE membrane, 5/pkgnatural ††

5056 0.45 µm PTFE membrane, 5/pkgnatural ††

5051 1.0 µm glass fiber media, 5/pkgnatural †

5053 3.0 µm glass fiber 5/pkgmedia/0.2 µm Bio-Inert membrane, natural

5062 Mustang Q ion exchange 5/pkgmembrane, natural

5063 Mustang S ion exchange 5/pkgmembrane, natural

AcroWell™ 96 Filter Plates, 350 µL WellPart Number Description Packaging

5020 0.45 µm GHP membrane, natural, 10/pkg350 µL well

5021 0.45 µm GHP membrane, white, 10/pkg350 µL well

5022 0.2 µm BioTrace™ NT 10/pkgmembrane, white

5025 0.2 µm BioTrace NT membrane, 10/pkgblack

5023 0.45 µm BioTrace PVDF 10/pkgmembrane, natural

5026 0.45 µm BioTrace PVDF 10/pkgmembrane, black

5027 0.45 µm BioTrace PVDF 10/pkgmembrane, white

AcroPrep 384 Filter Plates, 100 µL WellPart Number Description Packaging

5076 10K Omega™ membrane, 10/pkglong tips, natural

5077 10K Omega membrane, 10/pkgshort tips, natural

5078 30K Omega membrane, 10/pkglong tips, natural


5080 100K Omega membrane, 10/pkglong tips, natural


5070 0.45 µm GHP membrane, 10/pkglong tips, natural

5071 0.45 µm GHP membrane, 10/pkgshort tips, natural

5072 1.0 µm glass fiber media, 10/pkglong tips, natural

5072W 1.0 µm glass fiber media, 10/pkglong tips, white

5073 1.0 µm glass fiber media, 10/pkgshort tips, natural

5073W 1.0 µm glass fiber media, 10/pkgshort tips, white

5084 1.2 µm Supor membrane, 10/pkglong tips, natural

5085 1.2 µm Supor membrane, 10/pkgshort tips, natural

Vacuum ManifoldPart Number Description Packaging

5017 Multi-well plate vacuum manifold 1/pkg

5014 1 mL receiver plate spacer block 1/pkg

5015 350 µL receiver plate 1/pkgspacer block

5016 Replacement accessory kit 1/pkg(includes O-ring, gasket,allen wrench)

AccessoriesPart Number Description Packaging

5225 Adapter collar for centrifugation 2/pkg

5230 96-well plate cap mats 5/pkg

5231 Multi-well plate lids 10/pkg

Centrifugal devices

Nanosep® Centrifugal Devices,Omega MembranePart Number Description Packaging

OD003C33 3K, gray 24/pkg



OD010C33 10K, blue 24/pkg



OD030C33 30K, red 24/pkg



OD100C33 100K, clear 24/pkg



OD300C33 300K, orange 24/pkg



Nanosep MF Centrifugal Devices,Bio-Inert MembranePart Number Description Packaging

ODM02C33 0.2 µm, aqua 24/pkg



ODM45C33 0.45 µm, wildberry 24/pkg



Nanosep MF Centrifugal Devices,GHP MembranePart Number Description Packaging

ODGHPC34 0.45 µm, clear 100/pkg

ODGHPC35 0.45 µm, clear 500/pkg

Microsep™ Centrifugal Devices,Omega MembranePart Number Description Packaging

OD001C41 1K, yellow 24/pkg








OD050C41 50K, green 24/pkg






OD990C41 1000K, purple 24/pkg


Microsep MF Centrifugal Devices,Bio-Inert MembranePart Number Description Packaging





Macrosep® Centrifugal Devices,Omega MembranePart Number Description Packaging













† PTFE plates have polyester support.†† Glass Fiber plates have polypropylene support.

32

Ordering InformationCentrifugal devices

Macrosep® Centrifugal Devices,Omega™ MembranePart Number Description Packaging













Jumbosep™ Centrifugal Device Starter KitsPart Number Description Packaging

FD000K65 Generic starter kit, 4/pkg(no membrane inserts)

FD003K65 3K starter kit, gray 4/pkg

FD010K65 10K starter kit, blue 4/pkg

FD030K65 30K starter kit, red 4/pkg

FD100K65 100K starter kit, clear 4/pkg

FD300K65 300K starter kit, orange 4/pkg

Jumbosep Centrifugal Device Membrane InsertsPart Number Description Packaging

OD003C65 3K membrane insert, gray 12/pkg

OD010C65 10K membrane insert, blue 12/pkg

OD030C65 30K membrane insert, red 12/pkg

OD100C65 100K membrane insert, clear 12/pkg

OD300C65 300K membrane insert, orange 12/pkg

Jumbosep Device Accessory ProductsPart Number Description Packaging

FD001X65 Filtrate receiver and cap 12/pkg

FD002X65 Sample reservoir and cap 12/pkg

FD003X65 Insert release 24/pkg

Stirred cell systems

10 mL Omega Membrane-cell PacksPart Number Description Packaging

OC001C30 1K, yellow 10/pkg

OC003C30 3K, gray 10/pkg

OC005C30 5K, tan 10/pkg

OC010C30 10K, blue 10/pkg

OC030C30 30K, red 10/pkg

OC050C30 50K, green 10/pkg

OC100C30 100K, clear 10/pkg

OC300C30 300K, orange 10/pkg

150 mL Omega Membrane-cell PacksPart Number Description Packaging

OC001C60 1K, yellow 5/pkg

OC003C60 3K, gray 5/pkg

OC005C60 5K, tan 5/pkg

OC010C60 10K, blue 5/pkg

OC030C60 30K, red 5/pkg

OC050C60 50K, green 5/pkg

OC100C60 100K, clear 5/pkg

OC300C60 300K, orange 5/pkg

Stirred Cell Generic Starter KitsPart Number Description Packaging

FC000K30 10 mL Generic Starter Kit 1 kit

FC000K60 150 mL Generic Starter Kit 1 kit

Enchant™ protein purification kits

Enchant Life Science Kit Albumin DepletionPart Number Description Packaging

5300- Enchant Albumin Depletion 25 ALBDEP Kit (25 Nanosep® 0.45 µm samples

GHP centrifugal devices,25 Nanosep filtrate tubes, 25 albumin-depleting discs, 6.25 mL binding/wash buffer)

Enchant Life Science Kits IgG PurificationPart Number Description Packaging

5300- Enchant Protein A IgG 50IGGPROA Purification Kit (5 Protein A purifications

affinity purification columns,5 desalting columns, 1 liter Protein A binding buffer,500 mL Protein A elution buffer)

5300- Enchant Protein G IgG 10 IGGPROG Purification Kit (1 Protein G purifications

affinity purification column,5 desalting columns, 240 mL Protein G binding buffer,120 mL Protein G elution buffer)

Enchant Multi-Protein Affinity Separation KitPart Number Description Packaging

5300- Enchant Multi-Protein Affinity 24AFFMPS Separation Kit (24 Nanosep purifications

centrifugal devices, 5 mL anti-HSA resin, 5 mL anti-IgG resin, 20 mL wash buffer, 20 mL elution buffer)

Omega Membrane Discs (25, 43, 47, 50, 62, and 76 mm provided 12/pkg; 90 and 150 mm provided 6/pkg)MWCO 25 mm 43 mm 47 mm 50 mm 62 mm 76 mm 90 mm 150 mm1K OM001025 OM001043 OM001047 OM001050 OM001062 OM001076 OM001090 OM001150

3K OM003025 OM003043 OM003047 OM003050 OM003062 OM003076 OM003090 OM003150







www.pall.com33

*One Minimate TFF accessory kit is included in each Minimate TFF capsule package.

Tangential flowfiltration products

Minimate™ TFF Capsules,Omega™ MembranePart Number Description Packaging

OAD65C12 650D 1/pkg

OA001C12 1K 1/pkg

OA003C12 3K 1/pkg

OA005C12 5K 1/pkg

OA010C12 10K 1/pkg

OA030C12 30K 1/pkg

OA050C12 50K 1/pkg

OA070C12 70K 1/pkg

OA100C12 100K 1/pkg

OA300C12 300K 1/pkg

OA500C12 500K 1/pkg

OA990C12 1000K 1/pkg

Minimate TFF Capsule Accessory ProductPart Number Description Packaging

88216 Minimate Fitting Kit* 1/pkgConsists of male luer to 3.2 mm (1/8 in.) hose barb, female luer to 3.2 mm (1/8 in.) hose barb,3.2 mm (1/8 in.) i.d. tubing,tubing screw clamp, tubing clamps, adhesive strips (loop and hook)

Minimate TFF SystemsPart Number Description Packaging

OAPMP110 115V AC 50/60 Hz 1/pkg

OAPMP220 230V AC 50/60 Hz 1/pkg

OAPMP220UK 230V AC 50/60 Hz 1/pkgwith UK plug

Minimate TFF Reservoir AssemblyPart Number Description Packaging

OARES110 Reservoir Assembly 1/pkg115V AC 50/60 Hz

OARES220 Reservoir Assembly 1/pkg230V AC 50/60 HZ

OARES220UK Reservoir Assembly 1/pkg230V AC 50/60 HZ with UK plug

LV Centramate™ TFF Holder and AccessoriesPart Number Description Packaging

FS003K10 LV Centramate cassette holder 1/pkg

FS007X01 Bronze nuts and washers 4/pkg

FS710M01 Pressure gauge assembly 1/pkg[2 needed (filtrate and retentate); 3 needed if monitoring required]

FS700X10 Male:male connector to attach 8/pkgpressure gauges to holder

LV Centramate Cassettes, Alpha™ Membrane Medium Suspended

MWCO EFA Screen Screen

10K 0.01 m2 (0.1 ft2) AS010C12P1 AS010C11P1

10K 0.02 m2 (0.2 ft2) AS010C12P2 AS010C11P2

LV Centramate Cassettes, Supor® Membrane Medium Suspended

Pore Size EFA Screen Screen

0.1 µm 0.01 m2 (0.1 ft2) PSM10C12P1 PSM10C11P1










LV Centramate Cassettes, Omega Membrane Medium Suspended

Pore Size EFA Screen Screen

1K 0.01 m2 (0.1 ft2) OS001C12P1 OS001C11P1

1K 0.02 m2 (0.2 ft2) OS001C12P2 OS001C11P2

3K 0.01 m2 (0.1 ft2) OS003C12P1 OS003C11P1

3K 0.02 m2 (0.2 ft2) OS003C12P2 OS003C11P2

5K 0.01 m2(0.1 ft2) OS005C12P1 OS005C11P1

5K 0.02 m2 (0.2 ft2) OS005C12P2 OS005C11P2

10K 0.01 m2 (0.1 ft2) OS010C12P1 OS010C11P1

10K 0.02 m2 (0.2 ft2) OS010C12P2 OS010C11P2

30K 0.01 m2 (0.1 ft2) OS030C12P1 OS030C11P1

30K 0.02 m2 (0.2 ft2) OS030C12P2 OS030C11P2

50K 0.01 m2 (0.1 ft2) OS050C12P1 OS050C11P1

50K 0.02 m2 (0.2 ft2) OS050C12P2 OS050C11P2

70K 0.01 m2 (0.1 ft2) OS070C12P1 OS070C11P1

70K 0.02 m2 (0.2 ft2) OS070C12P2 OS070C11P2

100K 0.01 m2 (0.1 ft2) OS100C12P1 OS100C11P1

100K 0.02 m2 (0.2 ft2) OS100C12P2 OS100C11P2

300K 0.01 m2 (0.1 ft2) OS300C12P1 OS300C11P1

300K 0.02 m2 (0.2 ft2) OS300C12P2 OS300C11P2

500K 0.01 m2 (0.1 ft2) OS500C12P1 OS500C11P1

500K 0.02 m2 (0.2 ft2) OS500C12P2 OS500C11P2

1000K 0.01 m2 (0.1 ft2) OS990C12P1 OS990C11P1

1000K 0.02 m2 (0.2 ft2) OS990C12P2 OS990C11P2

0.16 µm 0.01 m2 (0.1 ft2) OS994C12P1 OS994C11P1

0.16 µm 0.02 m2 (0.2 ft2) OS994C12P2 OS994C11P2

Centramate Cassette HoldersPart Number Description Packaging

FS001K10 Includes stainless steel 1/pkgCentramate cassette holder,assorted fittings, torque wrench, and socket

FS002K10 Includes polyethylene holder 1/pkgwith Centramate cassette,stainless steel top and bottom brace plates, assorted fittings,torque wrench, and socket

Centramate Sanitary Gauge Fitting PackagesPart Number Description Packaging

FS005K10 2-gauge fitting package consists 1/pkgof (8) 1/2 in. EPDM gaskets,(2) 1-1/2 in. EPDM gaskets,(2) 1/2 in. x 1-1/2 in. TC tees,(3) 1/2 in. TC to 1/4 in. ID tube barbed fittings, (8) 1/2 in. TC clamps, (1) 1/2 in. diaphragm valve, (1) filtrate manifold,(2) 0-60 PSIG glycerin-filled gauges, (3) 3/4 in. TC to 1/2 in.ID tube barbed fittings, and (2) 1-1/2 in. TC sanitary clamps

FS006K10 3-gauge fitting package consists 1/pkgof (10) 1/2 in. EPDM gaskets,(3) 1-1/2 in. EPDM gaskets,(3) 1/2 in. x 1-1/2 in. TC tees,(3) 1/2 in. TC to 1/4 in. ID tube barbed fittings, (13) 1/2 in. TC clamps, (2) 1/2 in. diaphragm valves, (1) filtrate manifold,(3) 0-60 PSIG glycerin-filled gauges, (3) 3/4 in. TC to 1/2 in.ID tube barbed fittings, and (3) 1-1/2 in. TC sanitary clamps

Centramate PE Fitting PackagesPart Number Description Packaging

FS007K10 2-gauge fitting package 1/pkgconsists of (2) 1/4 in. NPT nipples, (2) 1/4 in. NPT tees,(2) 1/4 in. NPT to 1/4 in.barbed fittings, (1) 1/4 in. NPT to 1/4 in. barbed elbow fitting,(2) PSIG glycerin-filled gauges,(1) 1/4 in. ID tubing, (2) 1/4 in.NPT to 1/2 in. barbed fittings,(5) stainless steel hose clamps,and (1) 1/4 in. NPT to 1/4 in.barbed tee fitting

FS008K10 3-gauge fitting package 1/pkgconsists of (4) 1/4 in. NPT nipples, (4) 1/4 in. NPT tees,(3) 1/4 in. NPT to 1/4 in.barbed fittings, (2) 1/4 in. NPT to 1/4 in. barbed elbow fittings,(3) PSIG glycerin-filled gauges,(1) 1/4 in. ID tubing, (2) 1/4 in.NPT to 1/2 in. barbed fittings,and (5) stainless steel hose clamps

34

Ordering InformationTangential flowfiltration products

Centramate™ Cassette Hardware SystemsPart Number Description Packaging

FS010K10 Centramate system, 1/pkg2-gauge includes PN FS001K10 and FS005K10

FS011K10 Centramate system, 1/pkg3-gauge includes PN FS001K10 and FS006K10

Centramate PE Cassette Hardware SystemsPart Number Description Packaging

FS012K10 Centramate PE system, 1/pkg2-gauge includes PN FS002K10 and FS007K10

FS013K10 Centramate PE system, 1/pkg3-gauge includes PN FS002K10 and FS008K10

Centramate Cassettes, Omega™ Membrane Fine Medium Suspended

MWCO Screen Screen Screen

1K OS001C10 OS001C12 OS001C11

3K OS003C10 OS003C12 OS003C11

5K OS005C10 OS005C12 OS005C11

10K OS010C10 OS010C12 OS010C11

30K OS030C10 OS030C12 OS030C11

50K OS050C10 OS050C12 OS050C11

70K OS070C10 OS070C12 OS070C11

100K OS100C10 OS100C12 OS100C11

300K N/A OS300C12 OS300C11

500K N/A OS500C12 OS500C11

1000K N/A OS990C12 OS990C11

0.16 µm N/A OS994C12 OS994C11

Centramate Cassettes, Supor® MembraneMedium Suspended

Pore Size Screen Screen

0.03 µm PSM03C12 PSM03C11






Centramate Cassettes, Alpha™ MembraneFine Medium Suspended

MWCO Screen Screen Screen

10K AS010C10 AS010C12 AS010C11

Ultrasette™ Device Packages,Omega Membrane

Suspended MWCO Screen Channel Screen

1K, yellow OS001C70 OS001C72

3K, gray OS003C70 OS003C72

5K, tan OS005C70 OS005C72

10K, blue OS010C70 OS010C72

30K, red OS030C70 OS030C72

50K, green OS050C70 N/A

70K, brown OS070C70 N/A

100K, clear OS100C70 OS100C72

300K, orange OS300C70 OS300C72

Device packages include (1) device in the MWCO of your choice, (2) storagecaps for feed/retentate, filtrate outlet cap,(2) tubing clamps, and 0.6 m (24 in.) of 4.8 mm (3/16 in.) tubing.

Ultrasette Device Accessory ProductsPart Number Description Packaging

FS002X70 Accessory kit consists of 1/pkg1.8 m (6 ft.) of PharMed* #24 feed/retentate tubing,0.6 m (24 in.) of 4.8 mm (3/16 in.) Tygon filtrate tubing, (8) stainless steel tubing clamps, (1) screw clamp, and (1) barbed fitting

FS005X70 Gauge fitting package 1/pkgconsists of (1) 0 - 4.1 bar (0 - 60 psi) 3.2 mm (1/8 in.) NPT pressure gauge,(2) 6.4 mm (1/4 in.) O.D.polypropylene barbed tube to 3.2 mm (1/8 in.) NPT connectors, (1) 3.2 mm (1/8 in.) threaded polypropylene tee,(1) screw clamp,(2) stainless steel tubing clamps

FS001X70 Mounting bracket, 1/pkgholds Ultrasette device securely during operation; suction cups on the bottom of the bracket allow for placement on smooth surfaces

Ultralab™ Systems with 115 V PumpPart Number Description Packaging

FS006X75 2 L Ultralab system 1/pkgconsists of an Ultrareservoir container, Masterflex* L/S* variable speed peristalic pump, and Ultrasette accessory kit; connects to Ultrasette device sold separately

FS007X70 5 L Ultralab system 1/pkgconsists of an Ultrareservoir container, Masterflex L/S variable speed peristalic pump and Ultrasette accessory kit; connects to Ultrasette device sold separately

Ultralab Systems with 230 V PumpPart Number Description Packaging

FS016X75 2 L Ultralab system 1/pkgconsists of an Ultrareservoir container, Masterflex L/S variable speed peristalic pump, and Ultrasette accessory kit; connects to Ultrasette device sold separately

FS017X70 5 L Ultralab system 1/pkgconsists of an Ultrareservoir container Masterflex L/S variable speed peristalic pump, and Ultrasette accessory kit; connects to Ultrasette device sold separately

Ultrareservoir™ ContainersPart Number Description Packaging

FS005X75 2 L container includes 1/pkg0 - 4.2 bar pressure gauge,4.0 mm fittings, 6.4 mm fittings,and 3-way valve; suitable for use with Ultrasette device

FS006X70 5 L container includes 1/pkg0 - 4.2 bar pressure gauge,4.0 mm fittings, 6.4 mm fittings,and 3-way valve; suitable for use with Ultrasette device; fittings supplied are 6.4 mm

FS007X75 500 mL container includes 1/pkg0 - 4.2 bar pressure gauge,4.0 mm fittings, 6.4 mm fittings,and 3-way valve; suitable for use with LV Centramate system

www.pall.com35

Prefiltration and clarification

Acrodisc® PF Syringe Filters,Supor MembranePart Number Description Packaging

4187 0.8/0.2 µm, 25 mm, sterile 50/pkg

4658 0.8/0.2 µm, 32 mm, sterile 50/pkg

Acrodisc PSF GxF Syringe Filter, Glass FiberPart Number Description Packaging

AP-4523 GXF/Glass, 25 mm, 50/pkg,non-sterile 200/cs

Serum Acrodisc Syringe Filter,Supor MembranePart Number Description Packaging

4525 GF/0.2 µm, 37 mm, sterile 20/pkg

Acrodisc Syringe Filter, Glass FiberPart Number Description Packaging

4524 1 µm (nominal), 37 mm, 15/pkg,non-sterile 60/cs

VacuCap® 60 PF Device,Supor MembranePart Number Description Packaging

4638 0.8/0.2 µm, 60 mm, sterile 10/pkg

VacuCap 90 PF Device,Supor MembranePart Number Description Packaging

4628 0.8/0.2 µm, 90 mm, sterile 10/pkg

Protein detection products

Biodyne® A MembranePart Number Description Packaging

60113 0.2 µm, 30 cm x 3 m roll 1/pkg

60102 0.45 µm, 82 mm discs 50/pkg

60103 0.45 µm, 85 mm discs 50/pkg

60104 0.45 µm, 132 mm discs 50/pkg

60105 0.45 µm, 137 mm discs 50/pkg

60101 0.45 µm, 7 x 8.5 cm sheets 10/pkg

60100 0.45 µm, 20 x 20 cm sheets 10/pkg

60120 0.45 µm, 20 cm x 3 m roll 1/pkg

60106 0.45 µm, 30 cm x 3 m roll 1/pkg

60108 1.2 µm, 30 cm x 3 m roll 1/pkg

Biodyne B Membrane, 0.45 µmPart Number Description Packaging

60202 82 mm discs 50/pkg




60201 7 x 8.5 cm sheets 10/pkg

60200 20 x 20 cm sheets 10/pkg

60209 20 cm x 1 m roll 1/pkg

60208 20 cm x 3 m roll 1/pkg

60207 30 cm x 3 m roll 1/pkg

Biodyne C Membrane, 0.45 µmPart Number Description Packaging







60251 29 cm x 3 m roll 1/pkg

Biodyne Plus Membrane, 0.45 µmPart Number Description Packaging







60406 30 cm x 3 m roll 1/pkg

BioTrace™ NT Nitrocellulose TransferMembranePart Number Description Packaging







66485 30 cm x 3 m roll 1/pkg

FluoroTrans® MembranePart Number Description Packaging

PVM020C-160 7 x 8.4 cm sheets 10/pkg

PVM020C-195 8.5 x 9 cm sheets 20/pkg

PVM020C1015 10 x 15 cm sheets 10/pkg

PVM020C-196 13 x 14 cm sheets 10/pkg

PVM020C2020 20 x 20 cm sheets 10/pkg

PVM020C-099 26 cm x 3.3 m roll 1/pkg

FluoroTrans W MembranePart Number Description Packaging

BSP0158 7 x 9 cm sheets 10/pkg



BSP0161 26 cm x 3.3 m roll 1/pkg

BioTrace PVDF Transfer MembranePart Number Description Packaging



66547 20 cm x 1 m roll 1/pkg

66543 30 cm x 3 m roll 1/pkg

UltraBind™ Affinity MembranePart Number Description Packaging


66545 30 cm x 3 m roll 1/pkg

Immunodyne® ABC MembranePart Number Description Packaging

NBCH3RI 0.45 µm, 30 cm x 3 m roll 1/pkg

NNCH3RI 1.2 µm, 30 cm x 3 m roll 1/pkg

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7/06, PDF, GN05.1256 V2 PN 33411

Documents

Accelerate Protein Sample Preparation and Analysis · chromatography resins that exhibit high resolution and binding capacities with low non-specific binding for the purification