ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center Richmond Virginia 23219-1535 USA. *...
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ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center Richmond Virginia 23219-1535 USA. * ABTECH Scientific, Inc. Virginia Biotechnology Research Park 800 East Leigh Street, Suite 52 Richmond, Virginia 23219 USA Clinical Diagnostics Using Electroconductive Bio-Smart Thin Film Biosensors [email protected]http://www.abtechsci.com Anthony Guiseppi-Elie, Sc.D. A "Hands On" Workshop Theory and Practice of Point of Care Tests from Development Through Manufacturing Mar 31 - 2 Apr 2009.......... San Diego, CA
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center Richmond Virginia 23219-1535 USA. * ABTECH Scientific, Inc. Virginia Biotechnology Research
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. * ABTECH Scientific, Inc.
Virginia Biotechnology Research Park 800 East Leigh Street, Suite
52 Richmond, Virginia 23219 USA Clinical Diagnostics Using
Electroconductive Bio-Smart Thin Film Biosensors
[email protected]://www.abtechsci.com Anthony
Guiseppi-Elie, Sc.D. A "Hands On" Workshop Theory and Practice of
Point of Care Tests from Development Through Manufacturing Mar 31 -
2 Apr 2009.......... San Diego, CA
Slide 2
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. ABTECH Scientific, Inc. Founded
in 1995 and located in the Biotechnology Research Park in Richmond,
Virginia, ABTECH uses its platform electroactive polymer sensor
technology (EPST) to develop and deliver non- invasive,
near-patient molecular diagnostic products of clinical
significance.
Slide 3
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. ABTECH Scientific, Inc.
Laboratory Products Group Advanced Products Group
Microlithographically fabricated devices, related microelectrodes
(pH and Ref), flow cell products (gas and liquid) and instruments
that are used in research and development of electroconductive
polymer sensor technology. Non-invasive, near- patient molecular
diagnostic products of clinical significance and based on
electroconductive polymer sensor technology.
Slide 4
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Scope The presentation introduces
several of the device products of the Laboratory Products Group and
illustrates how these devices and substrates may be used in
chemical and biological sensor research and development around the
use of inherently electroconductive polymers.
Slide 5
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 5 Biosensors Defined
Classification of Biosensor Systems Based on molecular recognition,
transduction principles or physical placement Biosensor System
Systems Requirements Challenges in Biosensor Systems Development
and Application: Technical and Commercial Examples of Biosensor
Systems: Voltammetric Biosensor for lateral flow assay Implantable
Biochip for intramuscular monitoring during trauma Future of
Biosensor Systems Outline
Slide 6
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Biosensors are test and
measurement systems that include a biotransducer, which involves
biological recognition and signal transduction, and
instrumentation, which allows data capture and information
presentation, and is a format that allows the performance of rapid,
point-of- concern bioassays. Biosensors Defined The biosensor has
emerged as a suitable platform for the point-of-concern screening
and diagnostics in human and animal health, the environment and
national security.
Slide 7
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Biosensors Defined Biosensor --
Definition A biosensor is a measurement and/or monitoring system
that uses biologically active molecules, cell fragments, whole
cells or tissue as part of a biotransducer, which transforms
chemical information ranging from concentration of a specific
sample component to total composition analysis, into an
analytically useful signal. A biosensor is therefore a class of
chemical sensor Chemical Biosensor -- IUPAC Definition A chemical
biosensor is a device that transforms biochemical information
ranging from concentration of a specific sample component to total
composition analysis, into and analytically useful signal.
Slide 8
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. A Prototypical Biotransducer
Slide 9
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. A Prototypical Biosensor
System
Slide 10
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. The goal of biosensor systems is
to produce analytical data in context, i.e. useful information,
that allows the end-user to make decisions and take actions.
Competitive advantage Foot print (size of the device) Convenience
Large enough market to justify the cost of development Speed of the
test to be performed Why and Where Biosensor Systems are Used
Slide 11
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Sensor Semantics Detector vs.
Sensor A detector is generally not repeatable, is not updateable,
is not quantitative and not regenerable. A sensor is repeatable,
updateable, is quantitative and/or regeneragble. Advance, Confirm,
Clear Blue Easy, Answer, and ept Pregnancy Test Strips are
Detectors of Human chorionic gonadotropin (HCG) produced by the
placenta. Glucose biosensor for monitoring in critical care of
diabetes or monitoring of a bioprocess.
Slide 12
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 12 Broad Applications of
Biosensors Molecular diagnostics in patient health care measurement
and monitoring Blood metabolites (glucose, cholesterol),
therapeutic drugs (theophyline), drugs-of-abuse (cocaine). Food and
beverage industry Microbial pathogens in food (Salmonella ssp.)
Environmental and ecological monitoring Assaying of polychlorinated
biphenyls (PCBs) in soil samples Bioprocess monitoring Monitoring
of amino acids in mammalian cell culture
Slide 13
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Various Microarrays Formats
Affymetrix/Roche CYP450 Nanogen Osmetech CF GE Healthcare
Metrigenixs Flow-Thru 4-D Chip BCI SNPstream & Universal
Capture plate Combimatrix BioDots Lateral Flow & Dispensing
Amic Micronics lab card Brain Tumor beChip
Slide 14
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. ABTECHs Business and Technology
Space Enabling biochemical signal transduction with amplification
Uses electrochemical transducers (amperometric, voltametric,
impedimetric) Fashioned by microlithography with critical
dimensions less than 25 microns (defines microelectrode
electrochemistry) Focused on point-of-concern clinical molecular
diagnostics
Slide 15
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. microbore Microfabricated arrays
for electrochemical transduction Borosilicate Glass 1. Surface
Preparation 2. Photo Resist Application and soft bake Silicon
Nitride (Si 3 N 4 ) Sputtered Au (100 nm) on Ti/W (10 nm) 3.
Alignment and Exposure 4. Develop positive photoresist and post
bake 5. Fluro-plasma etch (Si 3 N 4 ) layer 6. Strip photoresist
layer
Slide 16
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Microfabricated Interdigitated
Microsensor Electrodes (IMEs) IME XX50.5 M, CD, FD; Au, Pt, ITO XX
= 05, 10, 15, 20 m IME XX25.3 M, CD, FD; Au, Pt, ITO M = Monolithic
CD = Combined Differential FD = Full Differential AFM Profile of 15
m IME
Slide 17
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Sensor Test Clip (STC-4)
Microfabricated Interdigitated Microsensor Electrodes (IMEs) Sensor
Test Clip (STC-7)
Slide 18
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Microfabricated Interdigitated
Microsensor Electrodes (IMEs) IME XX50.5 M, CD, FD; Au, Pt, ITO XX
= 5, 10, 15, 20 m
Slide 19
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Microelectrodes Oxygen
Microelectrodes pH and REF Microelectrodes
Slide 20
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. IME 1010.3 FD Au, Pt, ITO Liquid
Flow Cell Detector
Slide 21
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Schematic illustration of the IME
XX25.3 chips Collector Electrode Generator Electrode Digit width =
spacing = 5, 10, 15 & 20 microns)
Slide 22
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Photomicrograph and AFM Images
IME XX25.3 chips
Slide 23
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Ref: Analyst, 2007, 132, 365370 m
= number of the micro electrodes I = length of electrode, w =
width, and g = gap distance Signal amplification via redox cycling
IME XX25.3 chips
Slide 24
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Bioimmobilization of hydrogel
membranes onto IME chips
Slide 25
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Hydrogel Layer Glass substrate
SEM Photomicrograph of a Hydrogel Membrane of IME XX25.3 chips
Slide 26
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. MSRCVs obtained experimentally
with and without hydrogel coating on the IMEs in the presence of 1
mM Fc-COOH prepared in 0.1 M TRIS + 0.1 M KCl (pH = 7.2).
Slide 27
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Simulated (modeled) MSRCV for the
four IMEs with and without hydrogel in the presence of 1 mM Fc-COOH
following Butler-Volmer kinetics.
Slide 28
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Comparison of voltammograms of
IMEs at scan rate of 50 mV s -1
Slide 29
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. IME 1010.3 FD Au, Pt, ITO Gas /
Vapor Flow Cell Detector
Slide 30
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Chips of 5m, 10m, 15m and 20m
with electropolymerized PPy, PTh or PAn Sensor Arrays of VOC
Responsive Polymers for the Electronic NOSE
Slide 31
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. IAME-co-IME 2-1 Au, Pt
Independently Addressable Microband Electrodes and Microfrabricated
Interdigitated Microsensor Electrodes)
Slide 32
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 4 bands 3 mm long 2 m lines, 1 m
space 5 + 5 fingers 3 mm long 2 m lines, 1 m space IAME-co-IME 2-1
Au, Pt Independently Addressable Microband Electrodes and
Microfrabricated Interdigitated Microsensor Electrodes)
Slide 33
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. IAME-co-IME 2-1 Au, Pt
Slide 34
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. IAME-co-IME 2-1 Au, Pt
Independently Addressable Microband Electrodes and Microfrabricated
Interdigitated Microsensor Electrodes) 2 line 1 space
Slide 35
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. MDEA XXX Au, Pt Microdisc
Electrode Arrays 1
Slide 36
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. MDEA DEVICES Disc dimensions /
Number of Discs Active Area Conductor MDEA 3600 D = 3,600 m 1 disc
0.10 cm 2 Au, Pt, ITO MDEA 250 D = 250 m 207 microdiscs 0.10 cm 2
Au, Pt, ITO MDEA 100 D = 100 m 1,296 microdiscs 0.10 cm 2 Au, Pt,
ITO MDEA 050 D = 50 m 5,184 microdiscs 0.10 cm 2 Au, Pt, ITO 50
m100 m250 m x 50 3600 m x 50 Microdisc Electrode Arrays 2
Slide 37
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 50 m 100 m 250 m 3600 m Microdisc
Electrode Arrays 3 5,184 microdiscs 1,296 microdiscs 207 microdiscs
1 macro disc
Slide 38
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. PAnCl on MDEA 50 Au x50 PAnCl on
MDEA 100 Au x50 PAnPSS on MDEA 100 Au x50 PAnPSS on MDEA 50 Au x50
Total current density applied = 100 mA/cm 2 Total time = 20 s Total
charge density = 2 C/cm 2 Microdisc Electrode Arrays 4
Slide 39
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Independently Addressable
Microband Electrodes (IAMEs) XX = 5, 10, 15, 20 m Microband
Electrodes IAME XX04, Au, Pt or ITO
Slide 40
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Independently Addressable
Interdigitated Microsensor Electrodes (IAIMEs) XX = 5, 10, 15, 20 m
Microband Electrodes IAIME XX10, Au, Pt or ITO
Slide 41
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Design and Development of an
Electrochemical Lateral Flow Assay
Slide 42
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Introduction to Lateral Flow
Assays Definition: LFAs are devices designed to detect the presence
(or absence) of an analyte in a sample. Operating Principles:
Antibody-Antigen Capture Interaction; Capillarity
Slide 43
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Applications, advantages and
disadvantages of LFA LFAs are used in Home testing - Pregnancy
tests Rapid point of care testing HIV, Troponin T, Malaria, Drugs
of abuse, Fertility, Respiratory diseases. Human and animal
diagnostics Field testing of various environmental and agricultural
analytes Laboratory use Advantages User friendly format Very short
time to get test results Long term stability over wide climatic
ranges Relatively inexpensive to make Disadvantages Subjective
interpretation of results Non-quantitative at best Poor coefficient
of variation Lack of electronic documentation Sensitivity
limits
Slide 44
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Challenges with existing LFDs:
Opportunities for New Technologies Improved performance for Lateral
flow Improved sensitivity and specificity Improved
manufacturability Quantitative and semi-quantitative rapid tests
Patent pressures leading to innovation As new applications for
Lateral Flow Devices expand, there is demand for:
Slide 45
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Responding to the opportunity
with novel technology Improved quality of the flow field realized
through the use of proprietary fiber bed technology. Quantitative
detection realized through the use of proprietary microfabricated
array electroanalytical detection technology.
Slide 46
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Electrochemical Flow Through
Assay
Slide 47
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. A B C D E F G Electrochemical
Cell-on-a-Chip (ECC)
Slide 48
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Electrochemical Cell-on-a-Chip
(ECC) XX = 05, 10, 15, 20 m ECC IME XX04, Au, Pt or ITO 2 cm 1 cm
C1 C2 C2WE2 C2WE1 C2CE C1WE1 C1WE2 C1CE REF Schematic illustration
of a microfabricaed, dual-channel, electrochemical cell-on-a-chip
interdigitated microsensor electrode (ECC IME) device showing the
fluid flow over the microelectrode arrays. A=C2W2, B= C2CE, C=C2W1,
D=C1W1, E=C1CE, F=C1W2, G=REF. C2 is Cell 2, C1 is Cell 1, W is
working electrode, CE is counter electrode and REF is reference
electrode. A B C D E F G
Slide 49
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Counter Electrode Interdigitated
WE1 and WE2 Reference Electrode, Ag/AgCl Si 3 N 4 Passivation Layer
Unmodified Polypyrrole modified Unsilverized Silverized
Electrochemical Cell-on-a-Chip (ECC)
Slide 50
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. An integrated biochip showing the
microfabricated pattern of electrodes bonded and sealed into the
Microncs microfluidic T-cassette. Electrochemical Cell-on-a-Chip
(ECC) Integrated in a Microfluidic Cassette Polypyrrole
modified
Slide 51
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 1mM fcCOOH for 30 s at 200 s 1mM
fcCOOH for 10 s at 1350 s DI for 60 s at 1150 s Test Platform
Slide 52
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Design and Development of an
Implantable Biochip for Physiologic Status Monitoring during
Hemorrhage
Slide 53
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. PSMBioChip System (Physiologic
Status Monitoring) An Implantable Biochip for Physiologic Status
Monitoring 4 x 6 mm 4 x 1.5 x 0.5 cm Discrete Prototype Device ASIC
Device Glucose, Lactate, pH and Temperature
Slide 54
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. PSMBioChip Biotransducer PSM Dual
Microdisc Array Biotransducer Electrocond uctive Bio- smart
Hydrogel On Microdisc Array 2 mm 4 mm ECC MDEA 5037 A.
Guiseppi-Elie, S. Brahim, G. Slaughter and K. R. Ward, Design of a
Subcutaneous Implantable Biochip for Monitoring of Glucose and
Lactate, (2005) IEEE Sensor Journal, 5( 3), pp. 345-355.
Slide 55
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Si 3 N 4 Au Diffusion domain
Microdisc 2r Front-end biotransducer for discrete component
prototyping of the PSMBioChip 50x Counter electrode Reference
electrode Working electrode Electrochemical-Cell-on-a-Chip
Microdisc Electrode Array (MDEA) 50 m
Slide 56
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA.
Slide 57
ECC MDEA 5037 qualified by CV at 100 mV/s in 1.0 mM FcCOOH in
TRIS Buffer Two counter electrodes Two working electrodes Single
Reference electrode
Slide 58
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Performance enhancement of the
microdisc electrode array format of the PSMBioChip Hydrogel
CoatedUn-Coated G. Justin, A. R. Abdur Rahman, and A.
Guiseppi-Elie, "Bioactive Hydrogel Layers on Microdisc Electrode
Arrays: Cyclic Voltammetry Experiments and Simulations,"
Electroanalysis (2009) (accepted) Enhanced effective area with
reduction of disc diameter Enhancement maintained for 50 m device
beneath hydrogel
Slide 59
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. HydrogelTissue/Solution GOx Fe 2+
COOH Glucose Gluconolactone Lactate Pyruvate e- LOx Fe 2+ COOH e-
Au MDEA5037 Fabrication of the electrochemical
p(HEMA)/p(pyrrole)/Ox biotransducer AuSi 3 N 4 Glass Walter Torres
and Anthony Guiseppi-Elie, Simulations of Redox Mediation within
Bioactive Hydrogels of Amperometric Biosensors Journal of
Macromolecular Science, Part A: Pure and Applied Chemistry (2006)
(12) 1923 - 1928
Slide 60
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. Custom Spot Production Microarray
Array Fabrication BioDot AD 3400 Hydrogel Enzyme Cocktails
Slide 61
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA.
Slide 62
Slide 63
LOx or GOx Acr-PEG-NHS (Acr-PEG) n -GOx + ++
N-hydroxysuccinimide n Derivatization and functionalization of
(PEGylation) of oxidoreductase enzymes 0:1, 2:1, 4:1, 6:1, 8:1,
10:1, 12:1 Substrate (glucose / Lactate) RT, 2.5 h HEPES buffer n =
79; 113; 170 for 3,500; 5,000; 7,500 Zhang M, Li X. H, Gong Y. D,
Zhao N. M, Zhang X. F. Properties and biocompatibility of chitosan
films modified by blending with PEG. Biomaterials (2002) 2
3:2641-2648 Vamsi K. Yadavalli, Won-Gun Koh, George J. Lazur,
Michael V. Pishko Microfabricated protein-containing poly(ethylene
glycol) hydrogel arrays for biosensing Sensors and Actuators B 97
(2004) 290297.
Slide 64
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. In vitro Amperometric
Characterization of MDEA 5037 Biotransducer Sense Region 1
(Lactate) Sense Region 2 (Glucose) 4 mm 2 mm BioSTAT Dual
Potentiometer MDEA 5037
Slide 65
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. In vitro response of planar
glucose biosensor incorporating bio-smart hydrogel membrane of
composition 80:10:2.5:2.5:5.0 mol% (HEMA:TEGDA:PEGMA:MPC:Py) in 0.1
M PBKCl, pH 7.0 In vitro response of MDEA lactate biosensor
incorporating bio-smart hydrogel membrane of composition
80:10:2.5:2.5:5.0 mol% (HEMA:TEGDA:PEGMA:MPC:Py) in 0.1 M PBKCl, pH
7.0 In vitro response curves for glucose and lactate 50 m 100 m 250
m
Slide 66
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. PSM Biochip Monitoring of Glucose
and Lactate in Rats Real-Time In-Vivo Interstitial Fluid Blood
lactate and glucose concentration taken from drawn blood BioChip
capsule reading in the interstitial fluid of one rat
Slide 67
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. In vivo Amperometric performance
of the PSMBioChip IMTRAMUSCULAR BLOO D
Slide 68
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA.
Slide 69
69 Hemorrhage Remote Receiver Data Logger Ultimate
Demonstration : 1. Conscious Hemorrhaging Animal 2. Comparison of
VO 2 and Remote Glucose and Lactate Metabolic Chamber Signal
surgically implanted in rat model Glucose and Lactate Monitoring
and Data Transformation to oxygen debt Sensor- Transmitter PSM
BioChip Implant Capsule
Slide 70
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 70 Biosensor System Requirements
High degree of selectivity and sensitivity Rapid response
Repeatability/reproducibility Rapid recovery from insult Stability
Low drift Long lifetime Internal calibration and internal
referencing Reagentless - no auxiliary reagents required No sample
preparation Minimum cost per test+
Slide 71
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 71 Challenges in Biosensors
Systems Development and Application: Technical Stable, long-term
attachment of the bioactive layer to the solid state transducer
Stability of the bioactive layer in the biotransducer Reproducible
placement of small volumes of bioactive materials onto devices
Microarraying and microfluidic techniques. Photolithographic
patterning of bioactive layers Large scale manufacture - (The
I-Stat story) Biocompatibility of in vivo biosensors
Slide 72
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 72 Challenges in Biosensor
Systems Development and Application: Commercial Perceived marginal
cost benefit over established tests Cost of reagents - bioactive
reagents are expensive relative to typical reagents used in the the
chemical industry. Measurement culture - entrenched, protected
Pathologists, Environmental Scientists, Food Processors Integrated
biosensor systems that produce analytically useful data.
Understanding the end-user environment Distribution Channels
Slide 73
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. 73 Future of Biosensor Systems
Developments in manufacturability Pick and play; bio-inspired
approaches Microfabrication techniques applied to biosensor
production Photolithographic patterning of bioactive layers
Integration of biotransducers with microfluidic systems Sample
handling, preparation and delivery Improvement in sensitivity,
stability and selectivity Recombinant biomolecules Stabilization of
biomolecules in membranes and gels Integration of biosensors with
effector/actuator devices Controlled release
Slide 74
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. ABTECHs Patents A. Guiseppi-Elie;
US Patent No. 5, 766, 934; Issued on June 16, 1998. "Chemical and
Biological Sensor Devices Having Electroactive Polymer Thin Films
Attached to Microfabricated Devices and Possessing Immobilized
Indicator Moieties". A. Guiseppi-Elie; US Patent No.: 5, 352, 574;
Issued on: October 4th, 1994. "Electroactive Polymers with
Immobilized Active Moieties". A. Guiseppi-Elie; US Patent No.: 5,
312, 762; Issued on: May 17th, 1994. "Method of Measuring an
Analyte by Measuring Electrical Resistance of a Polymer Film
Reacting with the Analyte".
Slide 75
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA. People Scientific Advisory Board
Anthony Guiseppi-Elie, Sc.D. David Allara, Ph.D. Norman Sheppard,
Jr. Ph.D. Management Team James W. Sharpe, MBA Chairman and CEO
Anthony Guiseppi-Elie, Sc.D. President and Scientific Director
James R. Funkey, MA, CPA VP and Controller Ann M. Wilson, Ph.D.
Director, Applications Development Board of Directors James W.
Sharpe, MBA Chairman Anthony Guiseppi-Elie, Sc.D. President and
Scientific Director Annette Guiseppi-Elie, Ph.D. Secretary Cynthia
Garwon-Burke, Ph.D. Patent and IP Council Leigh P. Gregory Patent
Attorney Clemson, SC, USA
Slide 76
ABTECH Scientific, Inc. 800 East Leigh Street VA BioTech Center
Richmond Virginia 23219-1535 USA.