ABSTRACT Fruits of Romanian native plants from Adoxaceae family
(European Cranberrybush - Viburnum opulus L. E1 and European Black
Elderberry - Sambucus nigra L.-E2) have biological activity mainly
due to their high content of anthocyanins and other polyphenols.
They were used to obtain new nanomaterials based on gold
nanoparticles. We have controled the size, shape and surface
morphology of these nanomaterials (AuNPs-E1, AuNPs-E2) prepared by
using a green method; the new nanomaterials have been used in
medicine to treat some skin diseases. We have evaluated their in
vitro and in vivo effect and we have identified the effectiveness
of new topic therapies based on nanotechnology using high frequency
ultrasound. THE NON-INVASIVE ASSESSMENT OF THE EFFICACY OF GOLD
NANOPARTICLES-ANTHOCYANINS NANOMATERIALS ON CUTANEOUS PATHOLOGY
Liliana Olenic 1, Adriana Vulcu 1, Camelia Grosan 1, Luminita David
2, Bianca Moldovan 2, Maria Perde-Schrepler 3, Adriana Gabriela
Filip 4, Maria Crisan 4, Ioana Chiorean 5 1 National Institute for
Research and Development of Isotopic and Molecular Technologies,
Donath Street, Cluj-Napoca, Romania 2 Faculty of Chemistry and
Chemical Engineering, 11 Arany Janos Street RO , Cluj-Napoca,
Romania 3 Ion Chiricu Oncology Institute, Republicii Street
Cluj-Napoca, Romania 4 Faculty of General Medicine, Iuliu Haieganu
University of Medicine and Pharmacy Strada Emil Isac 13,
Cluj-Napoca, Romania 5 Faculty of Mathematics and Informatics,,
Babe-Bolyai University, Koglniceanu Street, 1, Cluj-Napoca, Romania
EXPERIMENTAL RESULTS AND DISCUSSIONS CONCLUSION Gold nanoparticles
functionalized with anthocyanins extracted from Viburnum opulus L.
and Sambucus nigra L. (AuNPs-E1 and AuNPs-E2) were prepared and
characterized by UV-Vis and FTIR spectroscopy and by Transmission
Electron Spectroscopy (TEM) (diameters between nm) There were
assessed cytotoxicity tests of the extract alone and of the
functionalized nanoparticles; To study the antiinflammatory effect,
keratinocytes treated with non-toxic doses of anthocyanins and
nanoparticles were exposed to increasing doses of UVB irradiation
The histograms of the skin thickness for 8 patients presented an
involution of diseases after treatment, fact that indicates the
very good antiinflammatory effect of our nanomaterials. Figure 1.
(a) Viburnum opulus L., berries; (b) Sambucus Nigra L., berries;(c)
And (d) TEM images of AuNPs with E1 respectively E2 INCDTIM Figure
2. UV-Vis spectra of (a) AuNPs-E1; (b) AuNPs-E2 Acknowledgement
This work was elaborated in the frame project no 147/2012, through
the program Partenerships in priority areas-PN II, developed with
the support of ANCS, CNDI-UEFISCDI 1. In vitro toxicity studies (a)
(b) (d) (c) Figure 3. The viability of HaCaTcell and A431 cells
function of nanomaterials concentration Figure 4. Compared of cell
survival between the two line of cells with the two types of
nanoparticles 2. In vivo toxicity studies Figure 5. Level of
malondialdehyde for different lots Figure 6.Glutatione level for
different lots Figure 7. Edema of foot after 2 h treatment 3.
Evaluation of new prepared nanomaterials on skin diseases Figure 8.
Images token with high-frequency ultrasound before and after
topical application of new nanomaterials for 2 weeks Figure 9. The
histogram of skin thickness for 8 patients before and after
treatment with nanomaterials
