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Bioremediation of selenium-contaminated environmental samples S. Hapuarachchi and T. G. Chasteen Department of Chemistry Sam Houston State University. Abstract - PowerPoint PPT Presentation
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Bioremediation of selenium-contaminated environmental samples
S. Hapuarachchi and T. G. Chasteen Department of ChemistrySam Houston State University
Abstract
A great deal of attention is now being focused towards the chemistry of toxic selenium in water and detoxification of selenium compounds. Selenium is an animal nutrient and has been used as anti-oxidant process but it can be fatal to living beings if a high amount of selenium exposure occurs. For an example, the accumulation of Se in the Kesterson Reservoir of California has been a serious threat to the animals around that area.
It is important to address environmental problems like this. Therefore, scientists have been trying to reduce the toxic nature of these selenium contaminated environmental sites by introducing detoxification methods. One of the detoxification methods currently being practiced is bioremediation. The reducing power of bacteria such as Pseudomonas fluorescens has been used to reduce the toxicity of soluble forms of selenium.
Understanding of the effectiveness of the bioremediation process is key to the improvement of this process. In this studies the distribution of selenium among three different physical states generated by a living bacteria culture and new ways to improve this bioremediation method will be discussed.
Introduction
What is Selenium? An element found in 1817 Name after Greek word, Selene, meaning “the moon”
Different forms of Selenium Metallic form (Se0 like carbon or solid iron or aluminum) Water soluble forms such as selenate and selenite Gaseous forms that will bubble out of solution, (CH3)2Se
Uses of Selenium Glass manufacturing industry Electronic applications such as rectifiers, solar batteries Use in plastics, paints, enamels, ink and rubber Semi-conductor materials
Environmental problems associated with selenium
Water contamination Power River Basin, Wyoming Kesterson Reservoir of California
Soil contamination Se contamination affecting plants and animals
Environmental Cleanup Methods
Biological Treatments
Filtration after pH adjustment
Evaporation and soil removal
Bioremediation
Different microbial pathways for the metabolism of toxic compounds facilitate the removal of these compounds from the environment.
Bacterium like Pseudomonas fluorescens can detoxify soluble selenium ions by reducing them to insoluble and other less toxic forms.
Aim of this study
Calculate the distribution of selenium among three different physical states generated by a living bacteria culture exposed to toxic forms of Se.
Modify this process to improve effectiveness of bioremediation process.
Experimentation
Bioreactor experiments Anaerobic culture growth
(without O2 present)
Sequential anaerobic/aerobic growth(without O2) followed by aerobic (with O2)
Sample analysis Inductively coupled plasma spectrometry
Results Mass balance with anaerobic culture growth
Se distribution (solid, liquid, gas) after 72 hrs of growth
Mass balance with mixed anaerobic alternating with aerobic growth
12 hrs anaerobic growth6 hrs aerobic growthTotal of 4 cycles (72 hrs total)
Phase % Recovery (SD)
Liquid 92.167(±8.31)
Solid 6.900(±1.32)
Gas 0.004(±0.002)
Total Recovery 99.071(±8.07)
Table 1. Ten mM of selenite (n=3)
Mass balance with anaerobic culture growth.
Results
% Recovery (SD)Phase
96.161(±0.62)Total Recovery
0.041(±0.07)Gas
32.440(±19.81)Solid
66.680(±18.29)Liquid
Table 2. One mM of selenite (n=6)
Mass balance with anaerobic culture growth.
Results
Table 3. Ten mM selenate (n=3)
Phase % Recovery (SD)
Liquid 95.067(±6.98)
Solid 0.733(±0.06)
Gas 0.001(±0.001)
Total Recovery 95.801(±6.93)
Mass balance with anaerobic culture growth.
Results
Table 4. Ten mM selenite (n=1)
Phase % Recovery
Liquid 80.934
Solid 6.337
Gas 0.001
Total Recovery 87.272
Mass balance w/ sequential anaerobic/aerobic culture growth.
Results
58.472Liquid
92.083Total Recovery
0.005Gas
33.606Solid
% RecoveryPhase
Table 5. One mM selenite (n=1)
Mass balance w/ sequential anaerobic/aerobic culture growth.
Results
Conclusions
Selenite was more effectively reduced by Pseudomonas fluorescens than selenate. (This may be because selenite is more toxic and getting rid of it as a solid is more useful.)
When low amounts of selenite are present in the solution, reducing efficiency is higher. (Because of toxicity, less selenite present may allow more detoxification to occur.)
Sequential anaerobic/aerobic culture growth does not have a big effect on this detoxification process as carried out. We saw no real difference in elemental Se product between cultures grown completely anaerobically as compared to mixed anaerobic and aerobic periods.