ABSTRACT

Determination of paracetamol and Codeine Phosphate simultaneously using thereversed phase High Performasce Liquid Chromatography (HPLC) have beenperformed. The mobile phase solution obtained can separates paracetamol fromcodeine phosphate with good resolution (RS = 2,704), this mobile phase were amixture of methanol and phosphate buffer 20 mM pH 2,5 (15:85). The condition ofHPLC used were Zorbax C8 column, temperature 27oC, flow rate 1,5 ml/minutes anddetected by DAD (λ = 284 nm). Linearity of paracetamol and codeine phosphate weregood (r paracetamol 0,9999 with Vxo = 0,51 % and r codeine phosphate 0,9999with Vxo 1,22 %). Precision instrument for paracetamol and codeine phosphatewere 0,77% and 0,79 % respectively. Accuracy of the methods were in the range ofaccepted limits ( 98 - 102 % ), average recovery of paracetamol and codeinephosphate in the simulation matrix were 99,99 % and 100,5 % respectively. Precisionmethods of paracetamol 0,64% and codeine phosphate 0,75%. The concetration ofparacetamol and codeine phosphate in sample tablet were 100,8% and 99,52%respectively, that fulfill of USP XXIV requairement ( 90 - 110 % ).

Keyword : HPLC, Paracetamol, Codeine phosphate, Optimation of mobile phase.