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FXR -/- + RSG. FXR +/+ + RSG. 250 µm. 250 µm. A. Weight of BAT. 0.7. Control. 0.6. **. RSG. ***. 0.5. 0.4. Weight (g). 0.3. C. 0.2. 0.1. 0. FXR +/+. FXR -/-. B. FXR +/+ - Control. 20. FXR +/+ + RSG. 15. Adipocyte fraction (%). 10. 5. 0. FXR -/- - Control. 20. - PowerPoint PPT Presentation
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Abdelkarim M et al.. Figure 1
B
A Weight of BAT
We
igh
t (g
)
FXR+/+ FXR -/-0
0.1
0.2
0.3
0.4
0.5
0.6** ***
0.7Control
RSG
Adipocyte diameter (µm)
Ad
ipo
cy
te f
rac
tio
n (
%)
0
5
10
15
20
0-20 20-40 40-6060-8080-100100-120120-140 140-160 160-180 180-200
0
5
10
15
20
Ad
ipo
cy
te f
rac
tio
n (
%)
FXR+/+ - ControlFXR+/+ + RSG
FXR-/- - ControlFXR-/- + RSG
C FXR+/+ + RSG FXR -/- + RSG
250 µm 250 µm
Abdelkarim M et al.. Figure 2
012345678
CD36 LPL
0
1
2
3
4
5aP2
0
1
2
3
4
0
1
2
3
4
5
6C/EBPβ
0
1
2
3
4
5
6C/EBPα
0
1
2PPARγ
0
1
2
3
4PERILIPIN
0
1
2
3
4ADRP
012345678
FSP27
Adiponectin
0
1
2
3
4
5
0
1
2
3
4S3-12
ControlRSG
ControlRSG
ControlRSG
FXR+/+ FXR-/- FXR+/+ FXR-/- FXR+/+ FXR-/- FXR+/+ FXR-/-
mR
NA
/Cy
clo
ph
ilin
mR
NA
/Cy
clo
ph
ilin
mR
NA
/Cy
clo
ph
ilin
FXR+/+ FXR-/- FXR+/+ FXR-/- FXR+/+ FXR-/-
FXR+/+ FXR-/- FXR+/+ FXR-/- FXR+/+ FXR-/- FXR+/+ FXR-/-
***
* **** *** ***
*
*
****
**** ***
C
A
B
Abdelkarim M et al.. Figure 3
mR
NA
/C
yc
lop
hil
in
0
C/EBPα
20
40
60
80
100
120
*
*
*
*
C/EBPβ
0
1
2
3
4
5
6
7
8FXR+/+
FXR-/-FXR+/+
FXR-/-
RSG
Days of Differentiation
mR
NA
/C
yc
lop
hil
in
0
10
20
30
40
50
**
*
*
PPARγFXR+/+
FXR-/-1000
1200aP2
0
200
400
600
800
**
**FXR+/+
FXR-/-
RSG + +- -D4 D8
+ +- -D4 D8
+ +- -D4 D8
+ +- -D4 D8
Abdelkarim M et al.. Figure 4
0
50
100
150
200250
300
350
D0 D2 D4 D8
*
*S3-12
*
0
1000
2000
3000
4000
5000
D0 D2 D4 D8
PERILIPIN
mR
NA
/Cy
clo
ph
ilin
FSP27
0
5000
10000
15000
20000
25000
30000
D0 D2 D4 D8
**
mR
NA
/Cy
clo
ph
ilin
02468
10121416
D0 D2 D4 D8*
*ADRP
Days of differentiation Days of differentiation
FXR+/+
FXR-/-
FXR+/+
FXR-/-FXR+/+
FXR-/-
FXR+/+
FXR-/-
C
µg
gly
ce
rol /
mg
pro
tein
Lipolysis
10
20
30
40
50
60
70
80
D4 D4+ISO D8 D8+ISO
***
**
Days of differentiation
FXR+/+
FXR-/-
mg
TG
/ m
g p
rote
in
10
12
14
16
18
D4 D8
2
4
6
8
D0
*TG content
Days of differentiation
FXR+/+
FXR-/-
Ace
tate
inco
rpo
rati
on
(d
pm
x1
04 ) /
mg
pro
tein
*
D0 D4 D8
5
10
15
20
25
30
**
de novo lipogenesis
Days of differentiation
FXR+/+
FXR-/-
Range of area
Cu
mu
late
d a
rea
(µm
2)
Size distribution of lipid droplets
0
1000
2000
3000
4000
5000
6000
<3 µm2
3-6 µm2
6-18 µm2
18-80 µm
2
>80 µm2
**
*
**FXR+/+
FXR-/-
B
0
50
100
150
200250
300
350
D0 D2 D4 D8
*
*S3-12
*
D0 D2 D4 D8m
RN
A/C
yc
lop
hili
nFSP27
0
5000
10000
15000
20000
25000
30000
D0 D2 D4 D8
**
mR
NA
/Cy
clo
ph
ilin
02468
02468
D0 D2 D4 D8*
*+/+
-/-
+/+
-/-
FXR+/+
FXR-/-
+/+
-/-FXR+/+
FXR-/-
+/+
-/-
FXR+/+
-/-
+/+
-/-
C
µg
gly
ce
rol /
mg
pro
tein
10
20
30
40
50
60
70
80 FXR+/+
FXR-/-
+/+
-/-
+/+
-/-
+/+
-/-
Ace
tate
inco
rpo
rati
on
(d
pm
x1
04 ) /
mg
pro
tein
*
D0 D4 D8
5
10
15
20
25
30
**
de novo lipogenesisFXR+/+
FXR-/-
Ace
tate
inco
rpo
rati
on
(d
pm
x1
04 ) /
mg
pro
tein
*
D0 D4 D8
5
10
15
20
25
30
**
FXR+/+
FXR-/-FXR+/+
FXR-/-
Cu
mu
late
d a
rea
(µm
2)
1000
µ2
-
2
µ2
-µ
2
+/+
-/-
B
A
Abdelkarim M et al.. Figure 5
Days of differentiation
B C
mR
NA
/Cyc
lop
hil
in
Days of differentiation
ADRP
0
5
10
15
20
25
30
D0 D4 D80
100
200
300
400
500
600
D0 D4 D8
*
PERILIPIN
FSP27
0
200
400
600
800
1000
D0 D4 D8
*
*
S3-12
D0 D4 D8
mR
NA
/Cyc
lop
hil
in
0
50
100
150
200FXR+/+
FXR -/-
FXR+/+
FXR -/-FXR+/+
FXR -/-
FXR+/+
FXR -/-
AaP2
D0 D4 D8 D0 D4 D8D0 D4 D8D0 D4 D8
mR
NA
/Cyc
lop
hil
in
0100200300400500600700800900
**
0
40
80
120
160
200
*
C/EBP
0
1
2
3
4
5
6
*
***
C/EBP
0
2
4
6
8
10
* *
PPAR
FXR+/+
FXR -/-FXR+/+
FXR -/-FXR+/+
FXR -/-FXR+/+
FXR -/-
aP2
D0 D4 D8 D0 D4 D8D0 D4 D8D0 D4 D8
mR
NA
/Cyc
lop
hil
in
0100200300400500600700800900
**
0
40
80
120
160
200
*
C/EBP
0
1
2
3
4
5
6
*
***
C/EBP
0
2
4
6
8
10
* *
PPARFXR+/+
FXR -/-FXR+/+
FXR -/-FXR+/+
FXR -/-FXR+/+
FXR -/-
FXR+/+
FXR -/-
Day 8Day 0
Days of differentiation
FXR+/+
FXR -/-
Day 8Day 0
mR
NA
/Cyc
lop
hil
in
ADRP
0
5
10
15
20
25
30
D0 D4 D80
100
200
300
400
500
600
D0 D4 D8
*
PERILIPIN
FSP27
0
200
400
600
800
1000
D0 D4 D8
*
*
S3-12
D0 D4 D8
mR
NA
/Cyc
lop
hil
in
0
50
100
150
200FXR+/+
FXR -/-
FXR+/+
FXR -/-FXR+/+
FXR -/-
FXR+/+
FXR -/-
Abdelkarim M et al.. Figure 6
0102030405060708090
100PPAR
0
200
400
600
800
1000
1200aP20
5
10
15
20
25
30C/EBPα
0
1
2
3
4
5
6
7C/EBPβ
0
5000
10000
15000
20000FSP27
0
5
10
15
20
25
30 S3-1202468
10121416
ADRP
0
20
40
60
80
100
120
140PERILIPIN
A BFXR-/- MEFs + empty retrovirus
FXR-/- MEFs + FXR retrovirus
Day 8Days of differentiation Days of differentiation
D0 D2 D4 D8 D0 D2 D4 D8
D0 D2 D4 D8 D0 D2 D4 D8Days of differentiation Days of differentiation
C
mR
NA
/Cy
clo
ph
ilin
mR
NA
/Cy
clo
ph
ilin
mR
NA
/Cy
clo
ph
ilin
mR
NA
/Cy
clo
ph
ilin
FXR-/- +empty retrovirusFXR-/- +FXR retrovirus
FXR+/+ +empty retrovirus
FXR-/- +empty retrovirusFXR-/- +FXR retrovirus
FXR+/+ +empty retrovirus
**
****
***
**
*
*
****
**
*
******
** ***
******
****
**
Abdelkarim M et al.. Figure 7Abdelkarim M et al.. Figure 7
C
D0 D2 D4 D8
Days of differentiation
D0 D2 D4 D8
FXR+/+ FXR -/-
c.i. acidred
Days of differentiation
D0 D2 D4 D8D0 D2 D4 D8
FXR+/+ FXR -/-
-catenin protein
-catenin
c.i. acidred
mR
NA
/Cyc
lop
hil
in
Cyclin
0
1
2
3
*
0
1
2
3
4
5c-Myc
*
mR
NA
/Cyc
lop
hil
in
0
1
2
3
Days of differentiation
0
1
2
3
4
5
*
D
mR
NA
/Cyc
lop
hil
in
LRP5
0
1
2
3
4
5
6
7
8
D0D1D2 D4 D8
* ***
Axin2
0
1
2
3
4
5
6
**
D1D2 D4 D8D0
mR
NA
/Cyc
lop
hil
in
0
1
2
3
4
5
6
7
8
D4 D80
1
2
3
4
5
6
D1D2 D4 D8D0
0
1
2
3
4
5
6
D0 D2 D4 D8D1
*sFRP5
0
1
2
3
4
5
6
D0 D2 D4 D8D1
*
mR
NA
/Cyc
lop
hil
in
sFRP1
0
5
10
15
20
D0 D2 D4 D8D1
*
mR
NA
/Cyc
lop
hil
in
0
5
10
15
20
D0 D2 D4 D8D1
* FXR+/+
FXR-/-FXR+/+
FXR-/-FXR+/+
FXR-/-
D0 D1D2 D4 D8 D0 D1 D2 D4 D8
mR
NA
/Cy
clo
ph
ilin
mR
NA
/Cy
clo
ph
ilin
β-catenin
mR
NA
/Cy
clo
ph
ilin
A B
*
0
1
2
3
4
FXR+/+ FXR-/-
LRP5
Axin2Cyclin D1
c-Myc
0
1
2
3
4
5
FXR+/+ FXR-/-
*
0
1
2
3
4
FXR+/+ FXR-/-
***
0
1
2
3
4
***
FXR+/+ FXR-/- 0
1
2
3
4
**
FXR+/+ FXR-/-
FXR+/+
FXR-/-FXR+/+
FXR-/-FXR+/+
FXR-/-
FXR +/+ -/- -/- +/+ -/- -/- +/+ -/- -/-
+ + - + + - + + -Empty retrovirus
FXRretrovirus
- - +- - +- - +
0
1
2
3
4c-Myc Axin2 LRP5
* **
* ** *
E
FXR +/+ -/- -/-
Empty retrovirus
FXRretrovirus
+ + -
- - +
c.i. acidred
-catenin
c.i. acidred
mR
NA
/Cyc
lop
hil
inm
RN
A/C
yclo
ph
ilin
Abdelkarim M et al.. Figure 8
D0 D4
FXR
sFRP1
Days of differentiation
c.i. acidred
- catenin
c.i. red
+/+ +/+
+-
-/- -/- -/-
- - -
A B
0
0.5
1
1.5
2
2.5
3
3.5
4
FXR +/+ -/- -/- +/+ -/- -/-
sFRP1 - - + - - +
PPAR aP2
***
**
![HIGH-RESOLUTION ESTIMATION OF ......crop reference ET, the basis for energy-driven components of the ETRM. Red represents high reference ET [Ketchum et al., 2016]. 29 Figure 4.2 Figure](https://img.pdfslide.us/doc/110x75/5fb354d0b3f74968ee78ba63/high-resolution-estimation-of-crop-reference-et-the-basis-for-energy-driven.jpg)
