References:

Wildanger, D. et al. “Solid Immersion FacilitatesFluorescence Microscopy with NanometerResolution and Sub-Ångström EmitterLocalization“, Adv. Mater. 24, 309 (2012)

Specs:

2-color 2D STED resolution < 30x30 nm(25x25 nm typical)

3D STED resolution < 100x100x100 nm,80x80x90 nm typical (with easy 3D STEDmodule)

Change between objectives (water/ oil/ glycerol immersion)

APD detection with highest detection sensitivity(>62% @ 680nm)

Pulsed STED laser and time gating

Combine with STED @595nm and RESOLFTfor up to 6 superresolution channels

Pulse / pixel / line / frame interleaved acquisition

Abberior multicolor STED @ 775 nm

Spectral properties and labels:

488 nm 594 nm 640 nm 775 nm Excitation wavelengths pulsed STED

Recommended labels:

Abberior STAR 520SXP, Abberior STAR 580, Abberior STAR 635P, Abberior STAR RED, ATTO 647N, ATTO 590, etc.

Confocal STED

RESCue mode optional

Göttfert, F. et al. “Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution“, Biophysical J.105, L01 (2013)

Harke, B. et al. “Three-Dimensional Nanoscopyof Colloidal Crystals“, Nano Lett. 8, 1309(2008)

Berning, S. et al. “Nanoscopy in a LivingMouse Brain“, Science 335, 551 (2012)

561nm520 nm

Aberration corrections for 2D and 3D STED