The tree-based progressive method for multiple sequence alignment, which utilizes:

(a) a tree (dendrogram) obtained by cluster analysis (b) pairwise alignment of 2 sequences’ alignments.

(a)

DEHUG3

DEPGG3

DEBYG3

DEZYG3

DEBSGF

(b) L W R D G R G A L Q

L W R G G R G A A Q

D W R - G R T A S G

L R R - A R T A S A

L - R G A R A A A E

(modified from Speed’s ppt presentation,see p. 81 in Kanehisa’s book)