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Paper Code (biotech12-08770720)
A novel and feasible viral transport media for SARS-CoV-2
diagnosis with prolonged preservation time
Saleh Javaher 1,2, Mohammad Arefkhani1,2, Alireza Fattahpour1,2, Hajarossadat
Ghaderi2, Atefeh Alipour3* and Hosein Shahsavarani1,2*
1. Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran
2. Lab. of Regenerative Medicine and Biomedical Innovations, Pasteur institute of Iran, Tehran, Iran
3. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran
Corresponding author’s email ([email protected];[email protected]
Introduction
1
Materials and Methods
2
Results and Discussion
3
Swab held at Fridge(4 °C) Swab held at 20–25 °C
Media Period 0 days 7 days 0 days 7 days 14days
Pasteur VTM Ct Value ND 26.0 25.1 27.0 28
our VTM Ct Value ND 25.8 26.3 26.0 26
Quantitative PCR (qPCR) was used to analyze inactivated SARS-CoV-2
Swab type Type Mfg Positive control Negative control
Hopes Flocked Hakiman Ofogh pars 25.6 ND
Good care Flocked China 26 ND
Goodwood Flocked China 27.1 ND
Traditional span type showed very weak detection capability compared to flocked type
Conclusion
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In conclusion, our proposed VTM formula proved robust for culture-based analyses provided the
inoculated VTM was stored at 4 °C, BUT It showed prolonged preservation time even for 2 weeks in
RT.
Based on obtained Ct values of spiking experiments, it was found that our VTM supported highly
consistent amplification of the SARS-CoV-2 target.
References
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McAuley, J., Fraser, C., Paraskeva, E. et al. Optimal preparation of SARS-CoV-2 viral transport medium for culture. Virol J 18, 53 (2021). https://doi.org/10.1186/s12985-021-01525-z
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