A great fermentation step, now what?

Mathieu Bailly / Daniel Bar

4th Bio-based Chemicals Summit Del Mar, CA January 29th, 2013

2

Established 1988 Shareholder 99% Tokuyama Corp. (Japan) Turnover 2012 26 MM € Total staff 60 EURODIA in North America Ameridia (New Jersey, USA) since 1999 EURODIA in Russia Eurodia Kuban (Krasnodar, Kuban State) EURODIA in China Lixus Separation Technologies (Shanghai)

EURODIA INDUSTRIE S.A.

A leading producer of specialty chemicals Established 1918 Turnover 2011 3 Billions USD Employees 4700 Subsidiaries 107 (including EURODIA)

TOKUYAMA CORPORATION

Eurodia / Ameridia designs and implements complete process solutions for purification and fractionation.

3

ADVANCED SEPARATION TECHNOLOGIES

Membrane filtration systems

(MF, UF, NF, RO)

Electrodialysis (conventional & bipolar)

Chromatography & ion exchange resins

COMPLETE PROCESS

SOLUTIONS

OUR TECHNOLOGIES

4

Whey processing / demineralization Proteins fractionation Milk standardization

Tartrate stabilization of wines and juices pH adjustment of wines and juices Alcohol removal from wines

4

MAIN INDUSTRIES DAIRY

WINE

OUR APPLICATIONS

Liquid sugar production Sugar decalcification Demineralization, clarification & decoloration of syrups Purification of specialty sugars and polyols Production of glucose & fructose Purification of oligosaccharides

STARCH AND SUGAR

5 5

OUR APPLICATIONS

MAIN INDUSTRIES (cont.)

Purification of organic acids Gluconic Acid Lactic Acid (PLA production / Bioplastics) Succinic Acid (Building block: BDO / PBS / others… Acetic Acid (Vitamins) Ketogulonic Acid (Vitamin C) others

Demineralization and purfication of amino acids

Glycine

Purification of plant proteins From peas From potatoes others

GREEN CHEMISTRY

6

EURODIA : INTERNATIONAL REFERENCES

Canada

USA

Mexico

Chile

Argentina

Brazil

South Africa

Australa

New Zealand

Russia

China

Europe

North Africa Middle East

Japan

GREEN CHEMISTRY ORGANIC ACIDS

The right purification process

• Meets the stringent purity requirements of the chemical industry

• Gives a high product yield • Has low operating costs • Minimizes the capital investment • Has the lowest effluent load The right process solution is often an optimized combination of several separation techniques

GREEN CHEMISTRY ORGANIC ACIDS

FERMENTATION

CLARIFICATION

PURIFICATION

CONCENTRATION

SUGARS

ORGANIC ACIDS

BIPOLAR MEMBRANE ED ION EXCHANGE RESINS CHROMATOGRAPHY NANOFILTRATION

CROSS-FLOW MF and UF

A good fermentation (or catalytic reaction) is only the start to obtain bio-based chemicals meeting the industry purity requirements .

The right purification process is key!

9

Initial product quality & quantity

Process solution

Final Product quality

Investment costs Production costs

Effluent specifications

Other constraints Selection based on know-how or from

R&D studies

Calculations based on dedicated files / data base

GREEN CHEMISTRY ORGANIC ACIDS

15 years of R&D programs and feedback from industrial

successes

GREEN CHEMISTRY ORGANIC ACIDS

FOCUS ON A GREEN TECHNOLOGY: BIPOLAR MEMBRANE ELECTRODIALYSIS (EDBM) An efficient production of acid and base from salts (e.g. Lactic acid / NH4OH from Ammonium Lactate) Driving force : electricity no chemicals, no effluents Recently: Development of high temperature EDBM (65°C)

• Minimization of energy footprint (by >25%) e.g. 0.73 vs 0.95 kWh/kg of lactic acid

• Improved control of bacterial contamination of DSP • Possibility to process viscous and specific streams (e.g. Succinic Acid)

GREEN CHEMISTRY ORGANIC ACIDS

BIPOLAR MEMBRANE ELECTRODIALYSIS (EDBM): PRINCIPLE « WATER SPLITTING » MEMBRANE

Bipolar

membrane

- OH- H+ H2O

CEL

AEL

+

GREEN CHEMISTRY ORGANIC ACIDS

BIPOLAR MEMBRANE ELECTRODIALYSIS (EDBM): PRINCIPLE TWO-COMPARTMENT STACK FOR ORGANIC ACID PRODUCTION

+ -

BP BP BP BP BP K K K K K K

K: cation exchange membrane BP: bipolar membrane

Cathode Anode OH- H+ OH- H+ OH- H+ OH- H+ OH- H+

Organic Salt (NaAc)

X- X- X- X- X-

Na+ Na+ Na+ Na+

Organic acid HX (+NaAc)

NaOH

Water

GREEN CHEMISTRY ORGANIC ACIDS

SOME EDBM INDUSTRIAL REFERENCES

GREEN CHEMISTRY ORGANIC ACIDS

ORGANIC ACIDS :THE FUTURE IS NOW…

GREEN CHEMISTRY ORGANIC ACIDS

THE KEY FOR PURIFICATION: COMBINING VARIOUS TECHNIQUES

Filtration Ceramic TAMI membranes / UF: to optimize DSP Organic membranes / NF: as part of polishing steps Ion Exclusion Chromatography Removal of salts, color, sugar, AA… Separation of organic salts from acids Ion Exchange Resins (conventional or continuous) Key Polishing steps to remove last traces of impurities Combination with complementary technologies: crystallization , evaporation, distillation, etc .

GREEN CHEMISTRY ORGANIC ACIDS

THE KEY FOR PURIFICATION: THE COMBINATION OF VARIOUS TECHNIQUES SOME INDUSTRIAL REFERENCES

GREEN CHEMISTRY ORGANIC ACIDS

CONCLUSIONS

• Eurodia/Ameridia has the expertise in bio-based organic acids to develop the most suitable downstream purification processes

• With our pilot facilities and simulation software, we have the tools to guarantee performance for our clients specific feedstreams

• We work under strict confidentiality obligations.

Thank you for your attention

www.ameridia.com