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SUPPLEMENTAL DATA. A convenient system for highly specific and sensitive detection of miRNA expression. Xiangqi Li, Minjie Ni, Chaobao Zhang, Wubin Ma, Yonglian Zhang. Fig. S1 Detection of miRNAs under different conditions by stem-loop PCR. miR-125a-3p. Marker. miR-122. miR-291. miR-326. - PowerPoint PPT Presentation
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A convenient system for highly specific and sensitive detection of miRNA expression
Xiangqi Li, Minjie Ni, Chaobao Zhang, Wubin Ma, Yonglian Zhang
SUPPLEMENTAL DATA
A
B
C
miR
-138
miR
-99b*
miR
-125
a-3p
miR
-291
miR
-292
-5p
miR
-295
miR
-326
miR
-308
5
NO miR
-122
Mar
ker
miR
-138
miR
-99b*
miR
-125
a-3p
miR
-291
miR
-292
-5p
miR
-295
miR
-326
miR
-308
5
NO miR
-122
Mar
ker
miR
-138
miR
-99b*
miR
-125
a-3p
miR
-291
miR
-292
-5p
miR
-295
miR
-326
miR
-308
5
NO miR
-122
Mar
ker
Fig. S1 Detection of miRNAs under different conditions by stem-loop PCR.
50bp100bp
50bp100bp
50bp100bp
Fig. S2 The sensitivity of the detection of miR-7578 and distinguishing miR-7578 from its precursor by northern blotting.
Rno-miR-7578
Pre-miR-7578
10 fmol
1 fmol
1 amol
1 zmol
JEG
-3 C
ell
MG
-63
Cell
U-2 O
S cel
l56
37 C
ell
T24 C
ell
Rno-miR-29a by aLHCD
Rno-miR-29a by northern blotting A
B
Fig. S3 Detection of miR-29a in different cell lines by different methods.
Fig. S4 Quantitative detection of miRNA levels using U6 as an internal control by LH-PCR.
Initial relative quantity
I1 I2 I8
Re
lativ
e q
ua
ntity
by
LH-P
CR
0
2
4
6
8
10
Fig. S5 Evaluation of LH-PCR amplification efficiencies of miRNA and its precursor.
RNA
Pre-miR-122 miR-122Re
lativ
e a
mp
lific
atio
n e
ffe
cie
ncie
s
0
20
40
60
80
100
120
miR-200b family
miR-200a miR-200c miR-200bRe
lativ
e a
mp
lific
atio
n e
ffe
cie
ncie
s
0
20
40
60
80
100
120
Fig. S6 Evaluation of LH-PCR amplification efficiencies of miRNA and its family members.
miR-29a
miR
-29a
miR
-29b
miR
-29c
Tissue RNA
Fig. S7 Detection of miR-29 family members by conventional Northern blotting.
Rno-miR-138
pre Hela liver kidey lung brain
Fig. S8 Detection of miR-138 and its precursor from tissue samples by stem loop-PCR .