Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143 S113

clusion, our findings showed that this lichen species can be used asa natural antimutagenic/anticarcinogenic agent.

http://dx.doi.org/10.1016/j.copbio.2013.05.347

Total phenolic, total flavonoid content and the DPPH free radicalscavenging activity of Lactarius deliciosus (L.)

Dilsad Onbasılı 1, Gökcen Yuvalı Celik 1, Hikmet Katırcıoglu 2,Ibrahim Narin 1

1 Erciyes University, Faculty of Pharmacy, Department of Pharmaceu-tical Biotechnology, Kayseri, Turkey2 Gazi University, Ankara, TurkeyE-mail address: odilsad@gmail.com (D. Onbasılı).

Lactarius deliciosus (L.) is a well-known mushroom which is widelyused as a food in Kastamonu province of Turkey. The search of newproducts with antioxidative properties is very active domain ofresearch. Mushrooms have been used as food and food-flavoringmaterial in soups and sauces for centuries due to their unique andsubtle flavor. L. deliciosus (L.), commonly known as the Saffron milkcap, red pine mushroom is one of the best known members ofthe large milk-cap genus Lactarius in the order Russulales. In thepresent investigation, L. deliciosus were used for quantitative esti-mation of the total phenolic contents as gallic acid equivalent (GAE)per gram dry weight and total flavonoid quantification were deter-mined by a colorimetric method. The antioxidative activity of theextract was elucidated by DPPH free radical scavenging capacityof the extract. As a result; DPPH scavenging activity of methanolicmushroom extract was found to be >17. The total phenolic contentand total flavonoid were found as 4.84 ± 0.32 mg GAE/g extract and2.76 ± 0.03 mg/g. In this study showed that the use of mushroomsnot only for their nutritional properties but also as a source for thedevelopment of drugs and nutraceuticals.

http://dx.doi.org/10.1016/j.copbio.2013.05.348

Antibacterial activity of ethanol extracts of mahaleb cherry(Prunus mahaleb L.) against some bacteria

Naciye Selcen Bayramcı 1, Demet Erdönmez 2, Yakup Budak 3,Özlem Egri 4

1 Department of Bioengineering, Faculty of Engineering and NaturalSciences, Gaziosmanpasa University, 60250 Tokat, Turkey2 Institute of Graduate Studies in Science, Division of Biol-ogy/Biotechnology, Hacettepe University, Beytepe, 06800 Ankara,Turkey3 Department of Chemistry, Faculty of Arts and Sciences,Gaziosmanpasa University, 60250 Tokat, Turkey4 Institute of Graduate Studies in Science, Division of Nanotechnol-ogy and Nanomedicine, Hacettepe University, Beytepe, 06800 Ankara,TurkeyE-mail address: selcenbayramci@hotmail.com (N.S. Bayramcı).

Different infections caused by gram-negative and gram-positivepathogens result in millions of illnesses worldwide. The mahalebcherry (Prunus mahaleb L.), a wild member of the Rosaceae fam-ily, is an important rootstock for cultivated cherry plants andits various parts are used as a spice and traditional medicine inTurkey. All the plant parts of mahaleb contain essential oil andcoumarins as the major secondary metabolites. The aim of thisstudy is to assess in vitro antibacterial activities of P. mahaleb L.ethanolic extract. The antibacterial activity of Prunus mahaleb L.fruits and seeds (core) ethanolic exracts was determined using discdiffusion and agar diffusion method against pathogens and non-

pathogen bacteria. The test bacterial strains were Escherichia coliDH5˛, Staphylococcus aureus USA 300, Pseudomonas aeruginosaPA14, Pseudomonas aeruginosa PA01, Agrobacterium tumefaciensA136, Chromobacterium violaceum 12472, Klebsiella pneumoniae,Vibrio harveyi, Bacillus subtilis 3610, B.thuringiensis, B.megatorium.It was found that the inhibition zone of tested bacteria againstP. mahaleb L. fruits, fruits and seed, and only seed (core) exractshave changed between 0 and 23 mm diameter of inhibition zone.The antibacterial activity of P. mahaleb L. fruits was not observed,but the seeds have shown antibacterial activity against all bacte-ria. Seed and fruit extracts are used with conjunction antibacterialactivity were increased.

http://dx.doi.org/10.1016/j.copbio.2013.05.349

In vitro antimicrobial, antioxidant and antibiofilm activities ofBryum capillare, a bryophyte sample

Dilsad Onbasılı 1, Gökcen Yuvalı Celik 1, Ergin Murat Altuner 2,Berrak Altınsoy 1, Belma Aslım 3

1 Erciyes University, Faculty of Pharmacy, Department of Pharmaceu-tical Biotechnology, Kayseri, Turkey2 Kastamonu University, Faculty of Science and Arts, Department ofBiology, Kastamonu, Turkey3 Gazi University, Faculty of Sciences, Department of Biology, Ankara,TurkeyE-mail address: odilsad@gmail.com (D. Onbasılı).

The aim of this study is to determine antimicrobial, antioxidant andantibiofilm activities of Bryum capillare (BC), a bryophyte sample.BC samples were collected subjected to ethanol extraction afterair drying. Antimicrobial activity was identified by determiningthe minimum inhibitory concentration (MIC) values. Antibiofilmactivity of the extracts was tested against Staphylococcus aureus,Staphylococcus epidermidis and Candida albicans. Total phenoliccompounds in the extracts were determined and antioxidant activ-ities of the samples were identified by DPPH radical scavengingassay, metal chelating activity on ferrous ions and plasma lipidperoxidation inhibitory assay. As a result of the study, the MIC val-ues were found to be ≥125 �g mL−1 against all strains. In addition,BC extract showed 5% antibiofilm activity against only S. epider-midis. Total phenolic compounds were determined as 23.26 mg g−1

and the results of DPPH radical scavenging activity, metal chelatingactivity on ferrous ions and plasma lipid peroxidation activity werefound to be 15%, 3% and 4% respectively. As a result, it can be con-cluded that although the antimicrobial, antioxidant and antibiofilmactivity of BC extracts were low, but further research is needed tobe conducted for a detailed investigation.

http://dx.doi.org/10.1016/j.copbio.2013.05.350

A comparative study on the antioxidant and antimicrobial activ-ity of Ganoderma lucidum extracts

Gökcen Yuvalı Celik 1, Dilsad Onbasılı 1, Berrak Altınsoy 1, BelmaAslım 2

1 Erciyes University, Faculty of Pharmacy, Department of Pharmaceu-tical Biotechnology, Kayseri, Turkey2 Gazi University, Faculty of Sciences, Department of Biology, Ankara,TurkeyE-mail address: gyuvali.11@gmail.com (G.Y. Celik).

The aim of the present study was to investigate antioxidative andantimicrobial effects of Ganoderma lucidum. Antioxidant activitiesof the ethanolic and methanolic extracts from G. lucidum were

S114 Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143

evaluated by using DPPH radical scavenging, metal chelating, totalflavonoid and total antioxidant activity assays. In addition, theamounts of total phenol, �-carotene, and lycopene componentsin the extracts were determined. DPPH radical scavenging effectwas detected in the methanol extract (IC50 = 3.82 ± 0.04 �g/ml) washigher than the ethanol extracts (IC50 = 7.03 ± 0.07 �g/ml). Com-pared to reference antioxidant, the methanol and ethanol extractsof G. lucidum provided a lower IC50 than BHA. Total phenol wasthe major antioxidant component found in the mushroom extracts.Using HPLC the phenolic compounds like apigenin, biochanin,catechin, quercetin, kaemferol, luteolin and rutin were detectedand quantified. Total antioxidant activity was measured accord-ing to ferric thiocyanate method. The results clearly showed thatmethanolic extracts had stronger total antioxidant activity thanethanolic extracts at the same concentration. The antimicrobialefficiency of the mushroom was evaluated according to microdi-lution broth methods. The ethanolic extract of G. lucidum having aMIC value 250 �g/ml, had the maximum antibacterial efficiencyagainst C. albicans ATCC 10231. These results showed that G.lucidum may be used in pharmaceutical applications because of itseffective antioxidant properties.

http://dx.doi.org/10.1016/j.copbio.2013.05.351

A comparative study of volatile oils extracting efficiency fromthree different mint species

Oana Munteanu 1, Iuliana Cretescu 2, Camelia Peev 1

1 “Victor Babes” University of Medicine and Pharmacy, Timisoara,Romania2 Banat’s University of Agricultural Sciences and Veterinary Medicine,Timisoara, RomaniaE-mail address: oanamnt@gmail.com (O. Munteanu).

Vegetal natural extracts containing pharmacologically active com-pounds are interesting in obtaining cosmetic and pharmaceuticalproducts. In the present work, most efficient use of aqueous andalcoholic extracts from three mint species was studied. To makebest use of each active component extracted, main purpose of thiswork was achieving the most advanced extraction of carbonyl com-pounds in mint.

To obtain aqueous and alcoholic extracts of Mentha arven-sis, Mentha crispa and Mentha × piperita, following materials wereused: grind leaves from these mint species, ethyl alcohol 96%,distilled water. The extracted plant material comes from controlcultures. Three different extraction methods were used: Soxhletextractor, normal reflux and maceration. Maceration providesvitamins, enzymes, volatile oils that would degrade by boiling.Refluxing in the short time (less than 3 hours) is done in order toextract active principles with average molecular weight, solublein hot alcohol and distilled water. The Soxhlet extractor is usedto extract in the short time (less than 5 hours) almost the wholerange of active principle. Obtained extracts were concentrated byevaporation of the solvent mixture with a rotatory evaporator.

Analyzing the volatile oil content of the extracts we can con-clude that the most effective types of extraction are:

• maceration method for Mentha arvensis (1.36% oil content ofactive principles extracted);

• method by refluxing for Mentha crispa (1.46% oil content of activeprinciples extracted);

• Soxhlet extractor method for Mentha × piperita (1.87% oil contentof active principles extracted).

http://dx.doi.org/10.1016/j.copbio.2013.05.352

Artemisia plant’s constituents HPLC identification and theirfungicide activity

Diana Monica Obistioiu 1, Klaus Stolze 2, Ivo Schmerold 2, Cos-min Ioan Tuns 1, Ileana Nichita 1, Eugenia Dumitrescu 1, DianaBrezovan 1, Romeo Teodor Cristina 1

1 USAMVB, Faculty of Veterinary Medicine, Timisoara, Romania2 University of Veterinary Medicine, Institute for Pharmacology andToxicology, Vienna, AustriaE-mail address: nimsavet@yahoo.com (C.I. Tuns).

Candidiasis is a current topic, Candida albicans being structuresable to show a strong and extensive action, expressed with specificmycoses whose evolution are, often, train/rebellious to treatment.

Aim of this study was to identify Artemisia plant constituentswith greater fungicidal effect than standard antifungal drugsused frequently in the treatment of candidiasis. Methodol-ogy included High Performance Liquid Chromatography (HPLC)method, which was used to identify five standard compounds:linalool, cineole, cariophyllene, thujone and eugenole within dif-ferent Artemisia essential oils. Also, the fungicidal effect of thesestandard compounds and their mixtures against clinical isolates ofC. albicans was followed using the Disc Diffusion Assay. Linaloolwas identified at 32.365 min; cineole is composed of an iso-mer mixture and has produced a series of five peaks at: 41.485,43.885, 44.780, and respectively 45.885 min. �-Thujone gener-ated a peak at 33.065 min, �-cariophyllene, at 42.832 min andeugenole at 27.740 min. Standards tested showed fungicidal effectas follows: eugenole > linalool > cariophyllene > thujone > cineole.Eugenole was by far the best antifungal standard tested, itsefficacy proving to be bigger by 32.2% to Nystatin and as amixture; mixture eugenole + linalool + cariophyllene + thujone hadan efficacy greater than Nystatin by +123.5%. After the in vitrotests done we can conclude that eugenol as single standardtested gave better results that the commonly used Nystatin butin mixtures its effect was +123.5% better that the allopathicdrug.

http://dx.doi.org/10.1016/j.copbio.2013.05.353

Effect of roscovitine on cardiac hypertrophy in human stem cellderived cardiomyocytes

Dimitar Trifonov 1, Hemanth Tummala 2, Sophie Clements 2, Niko-lai Zhelev 2

1 IMC University of Applied Sciences Krems, Krems, Austria2 University of Abertay Dundee, Dundee, Scotland, UKE-mail address: dimitartrifonov@imc-krems.eu (D. Trifonov).

Cardiac hypertrophy occurs as a response to a variety of stimuli(mechanical, hemodynamic, hormonal, pathologic etc) as the heartadapts to higher demands for cardiac work by increasing ven-tricular muscle mass. It has been shown that hypertrophy isaccompanied by activation of cyclin-dependent kinase 9, suggest-ing that CDK9 could be a suitable molecular target for therapeuticintervention. In this study we have investigated the effect ofroscovitine, a potent inhibitor of CDK2, CDK7 and CDK9 on car-diac hypertrophy. We have used a novel cell-based assay wherehypertrophy was induced in human stem cell derived cardiomy-ocytes by growth factors such as Angiotensin II and Endothelin-1.Our results demonstrate that roscovitine can prevent the inductionof cardiac hypertrophy through inhibition of CDK9. Furthermorewe have analyzed the effect of roscovitine on calcium dynam-ics in human cardiomyocytes. In addition, the perspective of