A

B

C

Ribosome

1) What is the difference between proteins A, B, C?

2) Where in the cell are these proteins synthesized?

3) The preparation of proteins B and C needs more steps than protein A. Why?

Copy the figure.

Functions of Membrane Proteins:

Transporter Enzyme Receptor

Cell Identity

Functions of Membrane Proteins(B)

Transport Proteins

Recognition

“Tags”

ReceptorsEnzym

es

C: A protein that is secreted by exocytosis.For example, Insulin (a hormone)

A Few General Facts:• Humans make about 30,000 different proteins (amino-acid chains).

• About 30% of these proteins are connected to the membrane.

• About 10% are secreted outside cells.

• The rest (60%) are water-soluble and function in the cytoplasm.

A

B

C

Ribosome

So, how do proteins get delivered to the membrane?!

Vesicular Transport Stolaf

Watch animation, use handout to mark the steps.

1) What is the purpose of the process?

2) What is happening in each step (6 steps)?

Vesicular Transport animation

“How Cells Operate?”

Work on the handout – coloring, short questions.

Can you now tell what each part of the cell is responsible for?

Membrane-bound organelles:

Golgi

Rough ER(with ribosomes)

Smooth ER(no ribosomes)

Cell Motion Music

Cell Motion Narrative

While you are watching:

What are the roles of:-Cytoskeleton-Nucleus-Ribosomes-Motor proteins

Click on “Watch video, high”

Red Blood CellsT-Helper Lymphocyte

Lymphocyte

Signal from another body cell

Lipid Raft: Cell, Stop!

Signal Transduction into cell interior

Decent into interior along microfilaments

Microfilaments are changing cell shape

Actin filament snapped into two by some enzyme.

Microtubules assembly

Micro-tubules disassembly- Net “movement”

Kinesin carries vesicle on microtubulue.

Contains proteins destined to the outside

Microtubule organizing center

Focus shifts from MOTC to the nucleus

mRNA shoots out of the nucleus

mRNA triggers the assembly of ribosomes

Ribosome translates a new protein that meantime folds up.

Protein is sent to the mitochondria

Another protein is translated and spinned into the ER

Vesicles carry new protein to Golgi.

Golgi vesicles carry protein to cell membrane

New protein is released to extra-cellular space

Lipid raft gathersaround new protein

Membrane proteins rise up

Proteins match and connect to opposite arteriole cell

As a result, arteriole cell makes space for white blood to exit.

Lymphocyte squeezes out to fight an infection.

Homework: Use page 79. On the cell coloring handout – Fill in the function of each organelle, and specify if it is related to:Protein synthesis and deliveryInformation centerEnergyStorage and waste disposalPhysical support