Order ID: 98061 Order Type: Enhance

PT. Genetika Science Indonesia,

Ruko Puri Mansion, Blok A No 19.

Jl. Lingkar Luar Barat Puri Kembangan.

Jakarta Barat, 11610, Indonesia.

Attn : Mr. Wiardi/ Sul Hidayah

Lane # SampleName SamType SamSize Volume Progress CommentsIf Insist Proceed,

Production Suggest

1 6 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

2 16 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

3 21 Unpurified PCR Product 347 20Wait customer

confirm

Too faint DNA band /DNA amount was too low to

be detected. If insist proceed, result will not be

guaranteed

Gel Extraction.

4 114 Unpurified PCR Product 347 20Wait customer

confirm

No band observed. If insist proceed, result will not

be guaranteed

Direct proceed cycle

sequencing without

purification

5 116 Unpurified PCR Product 347 20Wait customer

confirm

No band observed. If insist proceed, result will not

be guaranteed

Direct proceed cycle

sequencing without

purification

6 120 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

7 121 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

8 131 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

9 139 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

10 140 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

11 141 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

12 149 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

13 152 Unpurified PCR Product 347 20Wait customer

confirm

No band observed. If insist proceed, result will not

be guaranteed

Direct proceed cycle

sequencing without

purification

14 170 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

15 172 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

16 174 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

17 198 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

18 200 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

19 209 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

20 216 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

21 219 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

22 223 Unpurified PCR Product 347 20 Proceed Gel Extraction. Primer-dimers observed.

23 P13K_32 Unpurified PCR Product 188 20 Proceed PCR clean up.

24 P13K_43 Unpurified PCR Product 188 20 Proceed PCR clean up.

25 P13K_80 Unpurified PCR Product 188 20Wait customer

confirm

Too faint DNA band /DNA amount was too low to

be detected. If insist proceed, result will not be

guaranteed

Direct proceed cycle

sequencing without

purification

26 P13K_114 Unpurified PCR Product 188 20 Proceed PCR clean up.

27 P13K_120 Unpurified PCR Product 188 20 Proceed PCR clean up.

28 P13K_121 Unpurified PCR Product 188 20 Proceed PCR clean up.

29 P13K_124 Unpurified PCR Product 188 20Wait customer

confirm

Too faint DNA band /DNA amount was too low to

be detected. If insist proceed, result will not be

guaranteed

PCR clean up.

30 P13K_139 Unpurified PCR Product 188 20 Proceed PCR clean up.

31 P13K_140 Unpurified PCR Product 188 20 Proceed PCR clean up.

32 P13K_144 Unpurified PCR Product 188 20 Proceed PCR clean up.

33 9P13K_149 Unpurified PCR Product 188 20 Proceed PCR clean up.

34 9P13K_150 Unpurified PCR Product 188 20Wait customer

confirm

No band observed. If insist proceed, result will not

be guaranteed

Direct proceed cycle

sequencing without

purification

35 9P13K_157 Unpurified PCR Product 188 20Wait customer

confirm

No band observed. If insist proceed, result will not

be guaranteed

Direct proceed cycle

sequencing without

purification

36 9P13K_193 Unpurified PCR Product 188 20 Proceed PCR clean up.

37 9P13K_208 Unpurified PCR Product 188 20 Proceed PCR clean up.

38 9P13K_223 Unpurified PCR Product 188 20 Proceed PCR clean up.

Order ID: 98061

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 100bp

16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 100bp

Condition: 0.8% agarose gel

Amount of DNA ladder loaded per lane: 0.2ug each

Volume of Sample loaded per lane: 1uL each

100bp DNA Ladder (bp): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500

100bp DNA Ladder (ng/0.2ug): 16, 16, 14, 16, 34, 12, 11, 16, 16, 20, 29

Note: The DNA ladder is not applicable for sizing comparison of non-linear DNA samples (e.g. plasmid DNA)

31 32 33 34 35 36 37 38 100bp