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Michael R. Emmert-Buck, MD, PhD
Pathogenetics Unit
Laser Capture Microdissection Core
Laboratory of Pathology
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Specimen Workflow:
Patient or Model System
Tissue Processing
Molecular Analysis
Further Research;
Clinical Impact
Pathogenetics Unit
Laboratory MissionDevelop technologies for tissue analysis
Pathogenetics Unit
1. Laser Dissection
2. In Situ Methods
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NIH Collaborators:
Bob Bonner, Tom Pohida,
Paul Smith, Seth Goldstein,
Lance Liotta, Zhengping Zhuang
Tissue
Pathogenetics Unit
1991 2013Development Timeline
Arcturus PixCell
(First 10 Years)
Arcturus XT
(Second 10 Years)
Applications
Animal Model
Clinical Specimens
Plants
In vitro Cultures
Live Cell
LCM Cores
Over 100
Publications
~ 3,000
1991-95
Prototypes
1996-2001
LCM
2001-13
Improvements
2001-13
Applications
Example = LCM
As of 2013
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Tissue
Pathogenetics Unit
1991 2013Development Timeline
1991-95
Prototypes
1996-2001 2001-13
ImprovementsLCM
2004-13
Automation xMD
2004-13 2009-13
SIVQ
PGUMike Tangrea
University of Michigan
Ulysses BalisJerome Chang
NIH Collaborators
LCM Core
Applications
2001-13
Current Focus Automation xMD, SIVQ-LCM
1) High-throughput (xMD; SIVQ-LCM)
2) Precision (xMD)
3) Low Cost Kit (xMD)
4) Visualization (xMD)
5) Beta-testing - LCM Core; U-M (xMD; SIVQ-LCM)
6) Commercial Partner (xMD; SIVQ-LCM)
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1991 2013
Most ImportantAccomplishments
Pathogenetics Unit
Nathan - 2000 Emily - 2002 Alicia - 2005
1991-95
Prototypes
1996-2001
LCM
2001-13
Improvements
2001-13
Applications
2004-13
Automation
2004-13
xMD
2009-13
SIVQ
Development Timeline
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Tissue
Pathogenetics Unit
xMD, SIVQ-LCM
Applications,
Commercialize
2013 2031
Next Twenty Years
Clinical Utility of
Laser Dissection
??
Future questions to address
What is the role of laser dissection, if any, in
moleculardiagnostics?
Is there a compelling need?
Does cell purity matter in clinical assays?
- DNA mutation testing? NGS?- mRNA? miRNA?
- Proteomics?
Is there a need for a new meeting or working group?
Clinical
Microdissection
Working Group
NIH Collaborators
Mark Raffeld, ArmandoFilie, Liqiang Xi, LCM
Core
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Pathogenetics Unit
CollaboratorsLCM, xMD Extramural
Robert Bonner Ben Shapiro
Thomas Pohida Elisabeth Smela
Nicole Morgan Clinical Dissection WG
Don JohannLaboratory of Pathology
Avi Rosenberg SIVQ-LCM
Armando Filie Ulysses Balis
Mark Raffeld Jerome Chang
Liqiang Xi
Maria Merino MEN1
Elaine Jaffe Stephen Marx
Jason Hipp Sunita Agarwal
Stephen Hewitt
Mark Roth
