���� �� �� �� �� �� �� �� �� � � � � � � �� � � ���� � ���

� � � � � � � � � � � � � !" # $ $ % & ' ( ) * + , - . / 0 1

µ23 4 5 $ 6 7 ) * + * 0 8

µ23 4 5 $ 6 3 ) * + * 0 +

µ23 4 5 $ 6 ) ) * + * 0 9

µ23 4 5 $ 6 : ) * + / 0 ;µ

2" # $ " < & = = ) * + / 0 ;µ

2> ? @ A B C D E ? F ? G H B I J A C K E E L C K G ? C M D N M O M C ? L N M A L O I ? P L @ F EQ R S T U V W X U Y V Z X R V [ \ ] ^ _ ` a U Ub R c X S Y d e f Z Y [ g h Y i R V X i j X S Y k l f i Y j m n o ] k p ] qr Y U d Y j f i T j Y [ _ \ s QMPa

WHAT IS SUNSHELL?

THE NEXT GENERATION

HARDCORE SHELL PARTICLE

FEATURES OF SUNSHELL 2.6 µm AND 5 µm

. 1.6 µm and 3.4 µm of core and

0.5 µm and 0.6 µm of superficially

porous silica layer.

. Same efficiency and high throughput

as a Sub-2 µm and 3 µm particle.

. Same pressure as a 3 µm and

5 µm particles.

. Same chemistry as Sunniest

technology (reference figure below).

. Good peak shape for all compounds

such as basic, acidic and chelating

compounds.

. High stability (pH range for

SunShell C18, 1.5 to 10).

. Low bleeding.

t u vt w u v u v ttttu vt tu vtu vtu vu vt t t tu vt u v t u v u vt t u vtu v u vt tttu vtu vt t t t u v u vu vt tt tu vtu v t u v u vt t tttt u v t u v u v ttt t ttu vtu vt u v u vt w ttt u vt u v t u vtt ttu vt wtx y z { | } ~ �

� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � �SunShell C18 shows same efficien-

cy as a Sub 2 µm C18. In compar-

ison between fully porous 2.6 µm

and core shell 2.6 µm (SunShell),

SunShell shows lower values for

A term, B term and C term of Van

Deemter equation. The core shell

structure leads to higher perfor-

mance compared with the fully

porous structure.

Furthermore back pressure of

SunShell C18 is less than a half

compared to Sub-2 µm C18s.

0.5 µm

1.6 µm

2.6 µm

CORE

POROUS SILICA

Secure your analysis with SunShell hardcore column technology

Unique bonding technology combined with core shell particles gives you faster performance and more

reliable results. The SunShell technique assures top efficiency with all kinds of LC and UHPLC systems.

�

HOW DOES SUNSHELL WORK?

NARROW PARTICLE DISTRIBUTION

Wide particle distribution (Conventional silica gel D90/D10=1.50)

Narrow particle distribution (Core Shell silicaD90/D10=1.15)

A TERM

The size distribution of a core shell

(SunShell) particle is much narrower

than that of a conventional totally

porous particle, so that the space in

between the particles in the column is

reduced and efficiency increases by

reducing Eddy Diffusion

(multi-path diffusion) as the A term in

Van Deemter Equation.

VAN DEEMTER EQUATION

SUNSHELL

� � � � � � � � � � � � � �   ¡ � ¢ £ ¤ � ¥ � ¡ ¥ ¦ � � � § ¨ � � � ¦ � � � � � ©ª � � � � � « ¢ £ ¬ � �   � � £ ¦ ¨ � � � �   ¡ � ¢ £¤ ­ � � ¡ � � � �   ¡ � ¢ £ § ¢ � � � � § � � £ � ©® � � � � � ¯ ¦ ¡ ¡ � � ¦ £ ¡ � � �° ± ² ³ ´ ´ µ ¶ ´ · ¸ ¹ º ± ¶ » ¼ ² ½¾¿ À Á  ÃÄ À Á  à ŠÀ Á  ÃÆ Ç È É Á Á à À Á Â Ê Ë Ì À¤ � ¥ � ¡ ¥ ¦ � � � § ¨ � � � ¦ � � � � � ©

Í

B TERM

Diffusion of a solute is

blocked by the existence

of a core, so that a solute

diffuses less in a core

shell silica column than

in a totally porous silica

column. Consequently

B term in Van Deemter

Equation reduces in the

core shell silica column.

C TERM

As shown in the left figure, a core shell

particle has a core so that the diffusion

path of samples shortens and mass

transfer becomes fast. This means that

the C term in Van Deemter Equation re-

duces. In other words, HETP (theoretical

plate) is kept even if flow rate increas-

es. A 2.6 µm core shell particle shows

the same column efficiency as a totally

porous Sub-2 µm particle.

The right figure shows the diffusion

width of a sample in a 2.6 µm core shell

particle and a 2 µm totally porous par-

ticle. Both diffusion widths are almost

the same. The 2.6 µm core shell particle

is superficially porous, so that the dif-

fusion width becomes narrower than

particle size. Same diffusion means

same efficiency.

Î Ï Ð Ð Ñ Ò Ñ Ó Ô Ñ Õ Ð Ö Õ Ó × Ï Ø Ù Ú Ï Ó Û Ö Ú Ï Ð Ð Ù Ü Ï Õ Ó

Ý Þ ß à á â ã ä å æ æ á Þ ã Þ å ç è é ß ê ã è Þ ë ã à à è Þ ß á â Þ å ä ã ß æ é è ê â å ì à ã Þ í

î Õ Ø Û Ö Ö ï ð Õ Ò Õ Ù Ü Ü Ï Ö Ï Ô Ûñ Õ Ò Ñ Ü ò Ñ Ö Ö Ü Ï Ö Ï Ô Û Ý ì ß ê ã ë å â ó ß á â é ß ê ã Þ ô à ß ì õ Þ ä å æ æ á Þ å ß ç ß æ è Þ ß à á â ã í

ö ÷ ø ù ú û ü ý ÷ þ ÷ ÿ � ü ÿ ÿ � ý ü ÷ þ ù ú � � � � �µ � � � �

µ � �

µ� � �

µ�� � � � � � � � � � � �� � � � � �� � � � � � � � � � �� � � � � � � � � � � � � �� � � � � � !

" � �µ �C o re S h ell T o ta lly

p o rou s

# $ % % & ' $ ( )# $ % % & ' $ ( )* + , , - . - / 0 - + / 1 2 / 3 + 4 5 6 + / 7 1 6 + , , 5 8 + 2 /

* + , , 5 8 + 2 / 2 , 7 8 7 9 : 1 - + / 0 2 . - 8 ; - 1 17 / 6 4 2 4 7 1 1 < : 2 . 2 5 8 8 + 1 + 0 7

=> ?@ A@ BA == = C A = C > = C B = C ? @D E F G H I J K L M N O M P Q R

S T U V W X V V Y Y Z X V [ \ V ] ] S ^ _ ` ab c U V W X V V Y Y Z X V [ \ V ] ] S ^ _ ` ab c U V W X V V Y d Z e f ] ] g h Z X Z i [ S ` jkl mn op oq rp ns tu Y Z ] i a v w x i v x \ V ] ] Y y z { S ^ _µ

a T c | S ^ y a a} ~ � � � � � � ~ � ~ � � � µ� � � � � � � � �� ~ � � � � � � � � � � � � � � ~ � � � � � � � � � � � � � � � � � � � � �� � � � � � � � � � � � � � � � � �

  ¡ ¢ £ ¢ ¤ ¥ ¡ ¢ ¦ § ¨ £ © ¨ ª «   ¡ ¨ £ © ¬ ­ © ® ¯ ­ £

° ± ² ³ ² ´ ° ± µ ³ ¶ · ¶ ¸ ¹ · ³ º » ¼ ± ² ½ ¾ µ ³ ¶

¿

SUNSHELL

C18 - 2.6 µm

SUNSHELL C18 COMPARISON

À Á Â Ã Ä Å Æ À À Ç ÈÉ Ê Ë Ì Í Î ÄÀ Á Â Ã Ä Å Æ Ï À Ç ÈÐ Ì Ë Ç Í Î Ä

À Á Â Ã Ä Å Æ Ñ À Ç ÈÐ Ð Ë Ð Í Î ÄÒ Ó Å Ò Ô Õ Ö Ö À Ç ÈÐ Ç Ë È Í Î Ä× Ø Ù Ú Û Ü ÝÞ ß à á â ã Þ ã ß ã à ã á ã â ß Þ ß ßä å æ å ç æ è é ç æ è ê å ë ê è çÀ Á Â Ã Ä Å Æ ì À Ç ÈÐ Ð Ë í Í Î Ä

î ï ð Ç Ê Ë ñî ï ð ò Ë íî ï ð ò Ë Êî ï ð í Ë ñî ï ð ó Ë ñ× Ø Ù Ú Û Ü Ý× Ø Ù Ú Û Ü Ý× Ø Ù Ú Û Ü Ý× Ø Ù Ú Û Ü Ý

ô õ ö÷ ø ùú ï û ü ù ø× ØØ Ù Ú Û Ü Ý ô õ ö ý þ ÿ � � ô� � ù � � � þú ï û ü ù �× Ø Ù Ú Û Ü Ý� � ÿ � � ô � �� � ù � � � þ× Ø Ù Ú Û Ü Ýú ï û � ø ù ô õ ö ý þ� � ù � �ú ï û � ù ×× ØØ ÙÙ Ú Û Ü Ýô õ ö ý þ ÿ � � ô � �� � ù � � � þú ï û � ù × Ø Ù Ú Û Ü Ý

Mobile phase: CH3OH/H2O=75/25

Flow rate: 1.0 mL/min, Temperature: 40°ºC

Sample: 1 = Uracil, 2 = Caffeine, 3 = Phenol,

4 = Butylbenzene, 5 = o-Terphenyl,

6 = Amylbenzene, 7 = Triphenylene

Hydrogen bonding Hydrophobicity Steric selectivity

Company A C18 0.48 1.54 1.20

Company B C18 0.35 1.56 1.50

Company C C18 0.42 1.57 1.25

Company D C18 0.44 1.60 1.31

Sunshell C18 0.39 1.60 1.46

Retention of standard samples and back pressure were compared for five kinds of core shell type

C18s. Company A C18 showed only a half retention in comparison with SunShell C18. Steric

selectivity becomes large when ligand density on the surface is high. SunShell C18 has the largest

steric selectivity as well as the highest ligand density leading to the longest retention time.

HIGHEST RETENTION / LARGEST STERIC SELECTIVITY / LOWEST BACKPRESSURE

�

Amitriptyline overloads much more at

acetonitrile/buffer mobile phase than

methanol/buffer which causes tail-

ing. Five kinds of core shell C18s were

compared as refers to loading capacity

of amitriptyline. Thanks to the unique

bonding technology Sunshell gives ex-

traordinary peak shape, which means

better sensitivity and accuracy of the

method.� � � �� � � � � � � � � � � �� ! " ! # $ " ! # % & % # "

' ( ) � * � � ' ( ) � * � �' ( ) � * � �' ( ) � * � �' ( ) � * � �+ , - .+ , - .

/ 0 1 2 3 4 5 6 7 8 0 /9 : ; : < ; = > < ; = ? : @ ? = <A B C DE F G H I J K I L L MN B C DE F G H I J K I L L M

O P Q O K I L L B C DE F G H I J K I L L MO P Q Q R I J SB C D T U E V P L L W X G H G P J MB B C DE F G H I J K I L L MY B C DE F G H I J K I L L MZ [ \ 0 ] 0 7Z [ \ 0 ] 3 1Z [ \ 0 ] 1 4Z [ \ 1 ] 3 2Z [ \ 2 ] 1 0 Z [ \ 3 ] 2 7 A B C DE F G H I J K I L L MN B C DE F G H I J K I L L M

O P Q O K I L L B C DE F G H I J K I L L MB B C DE F G H I J K I L L MY B C DE F G H I J K I L L M+ , . ^ _ ` ^ a b c cd e f g b h a b ci j k l m l n o o p qd e f g b h a bi j k l m r s ^ _ `o p qi j k l m s t u o pd e f g bi j k s m r v i j k r m v n

i j k l m s w+ , - . ^ _ ` ^ a bd e f g b h ax y z { | } ~� � � � y � � | � � � � � � �� � � � � { � y � � | � | � � � � � � �� � � � � � � � { � � | � � } � � � � � � �� � � z � � � � { � � | � � } � � � � � � �

BEST PEAK SHAPE AVAILABLE

� � � � � � � � � � �  ¡ ¢ £ � ¤ � ¥ � ¤ ¦ � � � § ¨ © ª � « � � � « � � ¤ � � ¬ ­ ­ � ¦ « ® ¯ ° © ± ² © § ³ © ´ � � � � � � � � � � �  ¡ ¢ £ � ¤ � ¥ � ¤ ¦ � � � § µ © ª � � ª ª � ¥ � ¬ ª � £ � ¤ � ¤ � « ® ² ° ¶ ± ³ © § ² © ´Company A C18: Kinetex C18

Company B C18: Accucore C18

Company C C18: PoroShell C18 EC

Company D C18: Ascentis Express C18

¶

SUNSHELL C18 STABILITY

Stability under acidic pH condition was evaluated at 80°C

using acetonitrile/1% trifluoroacetic acid solution (10:90) as

mobile phase. 100% aqueous mobile phase expels from the

pores of C18 packing materials by capillarity and packing

materials do not deteriorate. Adding 10% acetonitrile to the

mobile phase enables accurate evaluation.

Stability under basic pH condition was evaluated at 50°C

using methanol/Sodium borate buffer pH 10 (30:70) as

mobile phase. Sodium borate is used as an alkaline stan-

dard solution for pH meters, which allows for a high buffer

capacity. Elevated temperature of 10°C reduces column life

to one third. The other company shows stability when tested

at ambient (room) temperature. If room temperature is 25°C,

column life is sixteen times longer than at 50°C.

�� �� �· �¸ �� � �� � � � � · � ¸ � � � � � � �¹ º» ¼½¾ ¿ºÀº½ ºÁ½¾ ÂÁà Ä

Å Æ Ç È É Êx Ë � x Ì } | | Í � ¸Í � z { y � � Î Í � ¸Í � z { y � � Ï Í � ¸Í � z { y � � � Í � ¸Í � z { � y � Í Í � ¸

Ð Ñ Ò Ó Ô Õ Ö × Ö Ø × Ù Ú Û Ü Ý × Ý Ú ÛÍ � | Ë z � Þ � ß } ~ � � à � � � z zá � â � | } { Ì y Þ } ~ Í ã ä Í � å � � � æ ç è � é { ã � � � � å ê �è | � ë � y � } ~ � � � z ì å z � �ç } z { } � y � Ë � } ~ ¸ � í Í

î ï ï ð ñ ð ò ó ô ð õ ó ï ö õ ö ï ô ð ÷ ôøù øú øû øü øý ø ø

ø ý ø ø ø ù ø ø ø þ ø ø ø ú ø ø ø ÿ ø ø ø û ø ø ø� � � � � � � � � � � � � �� � � � � � � � ý ü� � � � � � � � � ý ü� � � � � � � � � ý ü� � � � � � � � � ý ü� � � � � � � � � ý ü� �� �� !�"� �� �#$% &� '�% �()�(�* +

, - . / 0 1 2 3 2 4 3 5 6 7 8 9 3 9 6 7: ; < = > ? @ A B C D E F G H I J > >K ; L A < C M N O P C D: Q R S Q T H F > K @ ; U A = > L ; V O W C T J F > K X O S Q Y Z F T H J T [ \ ] M Q J F ^_ < ; ` V O W C D F I [ > a T > A ?b C > M C V O W = V C D E F c :

d e f g e h i j k j l kEXPANDED pH RANGE DUE TO

THE SUNSHELL BONDING TECHNOLOGY

m

TIC of Q1

BLEEDING TEST USING LC/MS

Column size: 50 x 2.1 mm

Mobile phase:

A) 0.1% Acetic acid

B) CH3CN

Gradient:

Time: 0min 1min 5min 7min

%B: 5% 5% 100% 100%

Flow rate: 0.4 mL/min

Temperature: 40 °C

MS: ABI API-4000 Ionization:

Turboionspray (cation)

Measurement mode:

Q1 Scan m/z 100-1000

The high stability of the SunShell columns also means low bleeding

in LC/MS analysis as shown here.

n o

SUNSHELL C18 EFFICIENCY

p q r s t u v w s u w x y r r p z { | } ~ � t z � � � � ~ � t tp q r s t u v w s u w x y r r p z { | } ~ � t � � � } ~ z t t

Sample: 1 = Uracil

2 = Toluene

3 = Acenaphthene

4 = Butylbenzene

Column: SunShell C18, 2.6 m 150 x 4.6 mm

Mobile phase: CH3CN/H2O=70/30

Flow rate: 1.0 mL/min

Pressure: 15.5MPa

Temperature: 25 ºC

UHPLC: Jasco X-LC� � � � � � �� � � �� � � � � � � � � � � � � � � � �

� r � � y � � � � � { | � � �� � � � � �

� r � � y � � � � z � | � � � Column: SunShell C18, 2.6 m 50 x 2.1 mm

Mobile phase: CH3CN/H2O=60/40

Flow rate: 0.3 mL/min

Pressure: 7 MPa

Temperature: 23 ºC

UHPLC: Jasco X-LC

� � � �� � � � � � � � � � � � � � � � �

ORDERING INFO OF SUNSHELLInner diameter

(mm)1.0 2.1 3.0 4.6 USP category

Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no

Sunshell C18, 2.6 µm

30

50

75

100

150

250

---

CB6141

---

CB6161

CB6171

---

CB6931

CB6941

CB6951

CB6961

CB6971

---

CB6331

CB6341

CB6351

CB6361

CB6371

CB6381

CB6431

CB6441

CB6451

CB6461

CB6471

CB6481

L1

EFFICIENCY = 253,000 plates/m

�   ¡ ¢ £ ¤ ¥ ¦ § ¨ © ª « ¦ ª ª�   ¡ ¢ £ ¤ ¥ ¦ § ¨ ¦ ¬ « ª ª ª

n n

SUNSHELL

Can be used in any L1 method - but with improved performance.

C18 - 5 µm

­ ® ¯ ­ ° ± ² ² ³ ´ µ ¶ · ¸ ¹º » ¼ ½ ¾ ¿ À­ ® ¯ ­ Á ± ² ²  ´ µ à ¹º ¼ ½ ¾ ¿» À Ä Å Æ Ç È É È È ÈÄ Å Æ Ê Ë É È È È Ä Å Æ Ë Ì É È È ÈÍ Î Ï ÐÑ Ò Ó Ô ÕÖ Ó Ô Õ

Totally porous silica

Sunniest C18, 5 µm

Core shell silica

SunShell C18, 2.6 µm

Core shell silica

SunShell C18, 5 µm

Specific surface area 340 m2/g 150 m2/g 90 m2/g

Retention

time (tR)

Retention

factor (k)

Retention

time (tR)

Retention

factor (k)

Retention

time (tR)

Retention

factor (k)

1) Uracil 1.70 0 1.34 0 1.30 0

6) Amylbenzene 19.96 10.74 16.56 11.36 13.43 9.33

Relative value of Amylbenzene 100% 100% 83% 106% 67% 87%

Column size: 150 x 4.6 mm

Mobile phase: CH3OH/H2O=75/25

Flow rate: 1.0 mL/min

Temperature: 40°ºC

Sample: 1 = Uracil

2 = Caffeine

3 = Phenol

4 = Butylbenzene

5 = o-Terphenyl

6 = Amylbenzene

7 = Triphenylene

HPLC: Hitachi LaChrom ELITE (Tubing, 0.25 mm i.d.)

There is a small difference

of k between totally porous

and core shell particles.

ORDERING INFO OF SUNSHELLInner diameter

(mm)1.0 2.1 3.0 4.6 USP category

Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no

Sunshell C18, 5 µm150

250

---

---

---

---

CB3371

CB3381

CB3471

CB3481L1

× Ø

SUNSHELL

C18 -WP / RP-AQUA / C8 / PHENYL / PFP - 2.6 µm

Column: SunShell C18, C18-WP, RP-AQUA, C8, Phenyl, PFP, 2.6

µm

150 x 4.6 mm

Mobile phase: CH3OH/H2O=75/25

Flow rate: 1.0 mL/min

Temperature: 40°ºC

Sample: 1 = Uracil

2 = Caffeine

3 = Phenol

4 = Butylbenzene

5 = o-Terphenyl

6 = Amylbenzene

7 = Triphenylene

Hydrogen bonding Hydrophobicity Steric selectivity

PFP 1.00 1.31 2.38

Phenyl 1.00 1.48 1.01

C8 0.32 1.46 1.08

RP-AQUA 0.52 1.52 1.30

C18-WP 0.40 1.55 1.35

Sunshell C18 0.39 1.60 1.46

Ù Ú Û Ü Ý Þ Ù Þ Ú Þ Û Þ Ü Þ Ý Ú Ù Ú Ú Ú Ûß à á à â á ã ä â á ã å à æ å ã â

ç è é ç ê ë ì ì í î íç è é ç ê ë ì ì ï ð

ç è é ç ê ë ì ì ï ñ ðò ó ô õ ö

÷ó ø ô õ ö ù

ù ÷ú û ü ú ý þ ÿ ÿ � �ò óô õ ö ù ÷ ç è é ç ê ë ì ì � í � � � � �ç è é ç ê ë ì ì ï ñ ð � � íò ó ô õ ö ù ÷ò ó ô õ ö ù ÷

ó ø ôòò õ ù ö ø ÷ ç è é ç ê ë ì ì í ê ë é � ìó ø ôò õ ù ö ø ÷ ú û ü ú ý þ ÿ ÿ ý

ULTIMATE SELECTIVITY

FOR YOUR ANALYSIS

�

ORDERING INFO OF SUNSHELLInner diameter

(mm)1.0 2.1 3.0 4.6 USP category

Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no

Sunshell C8, 2.6 µm

30

50

75

100

150

---

---

---

---

---

CC6931

CC6941

CC6951

CC6961

CC6971

CC6331

CC6341

CC6351

CC6361

CC6371

CC6431

CC6441

CC6451

CC6461

CC6471

L7

Sunshell PFP, 2.6 µm

30

50

75

100

150

---

---

---

---

---

CF6931

CF6941

CF6951

CF6961

CF6971

CF6331

CF6341

CF6351

CF6361

CF6371

CF6431

CF6441

CF6451

CF6461

CF6471

L43

Sunshell C18-WP, 2.6 µm

30

50

75

100

150

---

---

---

---

---

CW6931

CW6941

CW6951

CW6961

CW6971

CW6331

CW6341

CW6351

CW6361

CW6371

CW6431

CW6441

CW6451

CW6461

CW6471

L1

Sunshell RP-AQUA, 2.6 µm

30

50

75

100

150

---

CR6141

---

CR6161

CR6171

CR6931

CR6941

CR6951

CR6961

CR6971

CR6331

CR6341

CR6351

CR6361

CR6371

CR6431

CR6441

CR6451

CR6461

CR6471

Equivalent

to L62

Sunshell Phenyl, 2.6 µm

30

50

75

100

150

---

---

---

---

---

CP6931

CP6941

CP6951

CP6961

CP6971

CP6331

CP6341

CP6351

CP6361

CP6371

CP6431

CP6441

CP6451

CP6461

CP6471

L11

C18 -WP / RP-AQUA / C8 / PHENYL / PFP - 2.6 µm

�

SUNSHELL

� � � � � � �� � � � � � �� � � � � � � � � �(X: Cl, OCH3, OC2H5)

� �� �� � � �� �� � � � � � � �� � � � �� ���� ��� � �� � � � � � �� �� �� �� ��� � � � � � �� � ���� � � �� � � � �� � �� � �� � ��� � � � � � ��� � � ���� �! " # $ % " & ' ( ) * + ' , - . - & . * / * , - # $ 0 "12 . * % " & ' ( , + ' , - . - / * , $ 0 " 3 2 4 5 67 8 9 : ; < = > 8 ? > < @ A < ; B C A : < @ : D = 7 8 9 : ; < = > 8 ? > < @ A < ; B C = 9 : E = < = : B C F B D ? > D @B D E > G > 8 < E H A C < 8 :

HFC18 - 16 / HFC18 - 30 - 2.6 µm

High speed separations of proteins and peptides.

What is HFC18? Hexa-Functional C18 has six functional groups.

The HFC18 is much more stable under acidic conditions.

Proteins/peptides are often analysed

at acidic pH. The wide pore SunShell

phases are optimized for superior life

time at extreme conditions.

I J

KK L MK L NK L OK L PK L QK L RK L SK L TK L UM M K M K K M K K KV WXYZ[\] YV _` a Ybcd eef bd

g h i j k l m n j o j i p q n rs t u v w x v y y O K z {s t u v w x v y y M R z {

| } ~ � � � � � ~ � � � � � } � } � � } ~ � � � � � � � � ~ � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � �   � ¡ � ¢ � � � � � � � �£ ¤ ¥ ¦ § ¨ © ª © « ª ¬ ­ ® ¯ ° ª ° ­ ®± ­ ¨ ¤ ² ® ³ ´ ¤ ® ´ µ © ¨ ¨ ¶ · ± ¸ ¹ º ¸ » ¼ ½ » ² ¾ ¿ À Á ¼ ½ ¸ ² ²Â ­ § ° ¨ © à µ ¦ « © ³± ¶ Ä ± Å Æ À ½ ¸ Ç È ­ ¥ ² ° ¬ ¦ ¬ ° ¯ ¾ à ¶ ¼ ½ » É Ê À Æ » À· ¨ ­ Ë ¥ ¦ ª © ³ À ½ Ê ² Ì Æ ² ° ®Í © ² à © ¥ ¦ ª ¤ ¥ © ³ Î À Ï ±Â © ¦ « ¤ ¥ © ² © ® ª ¬ ­ ® ¯ ° ª ° ­ ®Â ­ § ° ¨ © à µ ¦ « © ³ ± ¶ Ä ± Å Æ ¶ Ð Ñ É » À Æ Ê À· ¨ ­ Ë ¥ ¦ ª © ³ À ½ Ê ² Ì Æ ² ° ®Í © ² à © ¥ ¦ ª ¤ ¥ © ³ Ê À Ï ±´ ¦ ² à ¨ © ³ ¸ É Ò ¥ ¦ ¬ ° ¨¼ É Ó ¤ ª Ô ¨ § © ® Õ © ® ©À¼ ÀÊ À» À¹ À¸ À À

À ¼ À À Ê À À » À À ¹ À À ¸ À À ÀÖ ×Ø ÙÚÛ Ü×Ý×Ú ×ÞÚÛ ßÞà áâã ä å æ ç è éê ë ì í î ï ð ñ ò ó ô ó ò õ ö ÷ ø ù ë úû ê ü ý þ ÿ � � � � �

ORDERING INFO OF SUNSHELLInner diameter

(mm)1.0 2.1 3.0 4.6 USP category

Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no

Sunshell HFC18-16, 2.6 µm

50

100

150

---

---

---

CG6941

CG6961

CG6971

CG6341

CB6361

CB6371

CG6441

CG6461

CB6471

L1

Sunshell HFC18-30, 2.6 µm

50

100

150

---

---

---

C46941

C46961

C46971

C46341

C46361

C46371

C46441

C46461

C46471

L1

HFC18 - 16 / HFC18 - 30 - 2.6 µm

� �

SUNSHELL

2 -EP (ETHYLPYRIDINE) - 2.6 µm

Figure 1

Figure 1: Chromatogram of the separation

for the 17-components mix using the Sun

Shell 2-EP 150 x 3.0 mm column. A meth-

anol gradient of < 2 minutes was used on

the Agilent 1260 Infinity SFC system. SFC

conditions: flow rate: 4.0mL/min; outlet

pressure 160 bar; column temperature

55°C. Gradient program: 5.0-7.5% in 0.20

min, then 7.5-20% in 1.3 min and held at

20% for 0.2 min.

The 2.6 µm core shell column shows

only one third of back pressure in com-

parison with the 1.7 µm fully porous

column. However, both show almost

the same efficiency. By such low back

pressure, a difference of density of su-

percritical fluid between an inlet and an

outlet of the column is reduced. Conse-

quently, 2.6 µm core shell column per-

forms a superior separation for SFC.

HARDCORE SFC SEPARATIONS

� �

Figure 2

Figure 2: Chromatogram of the separa-

tion for the 17-components mix using

Acquity UPC2 Viridis 2-EP 100 x 3.0 mm

column. 16 of the 17 components were

resolved. A methanol gradient of < 2

minutes was used on the Agilent 1260

Infinity SFC system. SFC conditions: flow

rate 3.5 mL/min; outlet pressure 160 bar;

and column temperature 70°C. Gradient

program: 5.0-12.5% in 1.0 min, 12.5% for

0.25 min, then 12.5-20% in 0.75 min.

Courtesy of Pfizer Inc.

ORDERING INFO OF SUNSHELLInner diameter

(mm)1.0 2.1 3.0 4.6 USP category

Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no

Sunshell 2-EP, 2.6 µm

30

50

75

100

150

---

---

---

---

---

CE6931

CE6941

CE6951

CE6961

CE6971

CE6331

CE6341

CE6351

CE6361

CE6371

CE6431

CE6441

CE6451

CE6461

CE6471

2 -EP - 2.6 µm

SUNSHELL

For Hydrophilic Interaction Chromatography.

Highly efficient separation of very polar compounds. Rapid equilibration.� � � � � � � � � � � � � � � � � � � � � � � � � ! � � � � � � � � " � # $ � � % �HILIC -AMIDE - 2.6 µm

The stationary phase of SunShell HILIC-Amide

consists of AMIDE and HYDROPHILIC GROUP,

so that this stationary phase is more polar than

an individual group. High speed separation is a

result of core shell structure that derives high

efficiency and fast equilibration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

ZY \ [X X Y Z [ \] ^ _ ] ` a b b c d e d f g h i j k a] f l i m n _ of l p a q ` a b b r l b o l bh f l i m n _ of l p a q ` a b b ] j b j s nt / H / + H 5 ' + H 5 * / I * 5 + u v w x y z { | w y v } v | } { ~ | ~ � � � } { z { | v � � � | � � v � � � � � � � � � � { z v � � ~ � v z� � � � { w � v y y v } v | } { ~ | � x � } ~ y } � x | � � ~ y v � � v � � � ~ � � ~ � �

ORDERING INFO OF SUNSHELLInner diameter

(mm)1.0 2.1 3.0 4.6 USP category

Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no

Sunshell HILIC-Amide, 2.6 µm

30

50

75

100

150

---

---

---

---

---

CH6931

CH6941

CH6951

CH6961

CH6971

CH6331

CH6341

CH6351

CH6361

CH6371

CH6431

CH6441

CH6451

CH6461

CH6471

L68

� �

SUNSHELL

� � � � � �� � � �   � ¡ ¢   � ¡ £ � ¤ £ ¡  ¥ ¦ § ¨ © ª« ¬ ­ ¬ ® ­ ¯ ° ® ­ ¯ ± ¬ ² ± ¯ ®³ ´ µ ¶ · ¸ ¹ º » ¼ ¸ ½¾ ¿ À ¾ Á  ¿ Ã Ä Å Æ Ç Â È Ç É ´ Ê Ë Ì Í Î Î ¶ Ì Ï Ì¾ ¿ À ¾ Á  ¿ Ã Ä Å Ð Ç Â Ñ Ç É ´ Ê È Ì Æ Î Î ¶ Ì Ï ÌÒ · ´ Ó Ê » Ô ¸ ½Ç Ì Ñ Î Õ Â Î ¶ Ö É ´ Ê Ë Ì Í Î Î ¶ Ì Ï ÌÍ Ì × Î Õ Â Î ¶ Ö É ´ Ê È Ì Æ Î Î ¶ Ì Ï ÌØ ¸ Î ¹ ¸ Ê » Ô Ù Ê ¸ ½ Ë Ú Û ¾Ü ¸ Ô ¸ Ý Ô ¶ ´ Ö ½ Þ ß à Ë Ú Ç Ö Îá » Î ¹ · ¸ ½ Í Å Þ Ê » Ý ¶ ·Ë Å Ø ´ · Ù ¸ Ö ¸Ñ Å â Ý ¸ Ö » ¹ º Ô º ¸ Ö ¸È Å ã Ù Ô ä · µ ¸ Ö å ¸ Ö ¸

æ ç è æ é ê ë ë ì í î ï ð ñ ò ó ô õ ö ð ñ í ó ó æ ç è æ é ê ë ë ì í î ï ð ñ ò ó í ô õ ö ÷ ñ ò ó óø ù ú û ü ý ú þ ý ÿ � � � ù � ú � �ø ù ú û þ ý ÿ � � � ù � ú � �

ø ù ú û ü ý ú þ ý ÿ � � � ù � ú � �ø ù ú û þ ý ÿ � � � ù � ú � �ú � � � � � � � ù �� � � � � � � �ú � � � � � � � � ù �� � � � � � � �

ú � � � � � � � � ù �� � � � � � � � � �ú � � � � � � � ù �� � � � � � � � � �� � � � � � � � � � � ! " # � � � �$ % & � � ' � � ( � ! " # � � � �

The filter is made of porous glass sized 4 mm i.d. and 4 mm thickness

Pore size is 2 µm

Low dead volume structure

Back pressure on glass filter is ca 0.1 MPa at 1.0 mL/min of flow rate

Upper pressure limit is more than 60 MPa

Available for 2.1 mm i.d to 4.6 mm i.d. columns

PROTECT YOUR COLUMNS

RP GUARD FILTER

ORDERING INFO OF SUNSHELL RP GUARD FILTER

(available as a guard column for reversed phase because of C18 bonding) No

Sunshell RP Guard Filter Starter Kit (holder, cartridge, tubing)

Sunshell RP Guard Filter for exchange (5 pcs)

Sunshell RP Guard Filter holder

CGGAAKN

CBGAAC

CBGAAH

) * + , , - . + / 0 1 2 3 4 , + 5 1 6

7 8 9 : ; < = ; > 8 < ;? > @ ; <A B C D E FG H I J K K L M NO G K K 9 ; E F @ PC 8 E : ; : Q D @ P R I S7 ; T I U K K

tel +43 (0)2572/4381 | fax +43 (0)2572/20791

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