5 VIG Alison & Stan [Kompatibilitätsmodus] · Ms Rasha ElMs Rasha El-Khadem AU ... 12 commercial cabbage lots (1 cv): ... 5 VIG Alison & Stan [Kompatibilitätsmodus] Author:

INTERNATIONAL SEED TESTING ASSOCIATION (ISTA) www.seedtest.org

Vigour Committee ReportVigour Committee Report

Zurich 2009


Vigour Committee Members: 2009

R i ti J h H t NZ A d L h lt DK

Ch i D Ali A P ll GB U it d Ki d

Resignations: John Hampton, NZ; Anders Lomholt, DK

Chair Dr Alison A Powell GB – United KingdomVice Chair Dr Michael Kruse DE - Germany

Ms Patricia Brownfield US – United StatesDr Emanuela Casarini IT ItalyDr Emanuela Casarini IT - ItalyDr Malavika Dadlani IN - IndiaDr Ibrahim Demir T - TurkeyMs Rasha El Khadem AU AustriaMs Rasha El-Khadem AU - AustriaDr Hulya Ilbi T - TurkeyDr Mohammad Khajeh Hosseini IR - IranProf Seok Hyeon Kim K – South KoreaProf Seok Hyeon Kim K South KoreaDr Tim Loeffler NL - NetherlandsMs Gillian McLaren GB – United KingdomDr Stan Matthews GB – United KingdomgMs Marie-Helene Wagner FR - FranceMs Yan Rong Wang CH - China


Outline• Method Development

– Development of new vigour tests– Addition of new species to validated tests– Research developments

• Method Validation

fi i• Proficiency tests

• Trainingg

• The future


Method Development

1 D l f i1. Development of new vigour testsRate of germination test: WG led by S Matthews

2. Addition of new species to validated testsC d i i bConductivity test: soyabean

3 Research developments3. Research developments


1. Development of new vigour tests: Rate of Germination TestWork on maizeWork on maize

Two publications (Matthews and Khajeh Hosseini, 2007, 2008; SST 34, 35): Rate (MGT) and soil emergenceRate (MGT) and soil emergence

One publication (Khajeh Hosseini et al, 2009; SST 37); poster at Brazilian Congress: Field emergence and repeatability 13oC, 3 labs

2006 – 2007 (Seed Symposium, Brazil): R bili i 4 l b (13 d 20 C) l d MGTRepeatability in 4 labs (13 and 20oC); early counts and MGTRelated to cold test and compared to AA (paper in preparation)

2007: Argentinian WG (AM Bologna): repeatability in 8 13 labs (13 and 20oC)2007: Argentinian WG (AM, Bologna): repeatability in 8-13 labs (13 and 20oC)Early counts and MGT related to field emergence

2007 -2008: Validation study - repeatability in 6 labs (13 and 20oC)2007 2008: Validation study repeatability in 6 labs (13 and 20 C)Early counts related to field emergence


V lid ti t dValidation study

L d St M tth (UK) R h El Kh d (AT)• Leaders: Stan Matthews (UK), Rasha El Khadem (AT)

• 6 participants:6 participants: – Italy (LaRAS)– France (SNES)– Austria (2 labs, Pioneer; AGES)– Iran (University of Ferdowsi)

UK (U i it f Ab d )– UK (University of Aberdeen)

• 9 lots screened Feb/ March 2007 (Iran, UK);9 lots screened Feb/ March 2007 (Iran, UK); 6 lots selected for comparative test; distributed April 08

• Assessment of rate of germination13oC; 20oC; 2 runs at each temperature


T bl C i f h i l i l i ti (%) ft 66 h t 20oC

Seed lot Laboratory Overall mean

Table: Comparison of mean physiological germination (%) after 66 h at 20oC.Most rapid germination, high vigour (ranked 1); slowest germination (ranked 6).

Seed lot Laboratory Overall mean

1 2 3 4 5 6

Run Run Run Run Run Run

1 2 1 2 1 2 1 2 1 2 1 2

12 95 97 93 94 98 100 100 99 98 99 99 100 98

1 1 1 1= 1 1 1 1= 1 1 1 1 1

4 92 96 90 94 90 94 97 99 96 98 94 94 95

2 2 2 1= 2 2 2= 1= 2 2 2 2 2

9 90 94 90 9 9 91 93 92 9 91 919 90 94 88 90 89 88 97 91 93 92 89 91 91

3 3 3 2 3 3 2= 5 3 3 4 3 3

5 65 72 84 74 67 62 95 94 91 91 93 82 81

4 4 4 4 4 5 4= 3 4 4 3 4 4

7 62 64 - - 65 66 78 88 71 83 59 65 70

5 5 5 4 4 6 5 5 5 5 55 5 - - 5 4 4 6 5 5 5 5 5

10 29 32 31 24 38 41 76 93 61 77 28 16 46

6 6 6 6 6 6 6 4 6 6 6 6 6


Other Crops:

P D i t l (2008) S d S i d T h l 36Pepper: Demir et al (2008); Seed Science and Technology, 36

Cucurbits: Mavi, Demir and Matthews, 2009;Cucurbits: Mavi, Demir and Matthews, 2009; submitted for publication

Cotton: with Cotton Seed Distributors in Australia


100 100

Cotton in Australia 2007

y = -20.255x + 133.84R2 = 0.83 ***

80

y = 0.3442x + 44.743R2 = 0.76 ***80

60

d em

erge

nce

60

d em

erge

nce

20

40

% F

iled

20

40

% F

iled

SlowOlder seed

SlowOlder seed

02 3 4 5

00 20 40 60 80 100

MGT (18 o C) d % Germination 3d (18 o C)

Slow germinating lots give low field emergenceLab tests: Aberdeen, UKField emergence: Australia

Slow germinating lots give low field emergence



C d ti it t t b

2. Addition of new species to validated tests

Conductivity test: soyabean

Leaders: Rasha El Khadem (AT), Alison Powell (UK)Leaders: Rasha El Khadem (AT), Alison Powell (UK)

5 seed lots from Italy, sent out May 2008

7 laboratories:UK (2 l b OSTS C b id d Edi b h)UK (2 labs: OSTS, Cambridge and Edinburgh) Italy (LaRAS)France (SNES)France (SNES)Iran (University of Ferdowsi)Turkey (Ege University)Netherlands (Seminis)


Comparison of conductivity readings from 7 laboratoriesRed numbers indicate seed lot ranking from 1 (high vigour) to 5 (low vigour)

Lab Lot Lab meansA B C D E

Red numbers indicate seed lot ranking from 1 (high vigour) to 5 (low vigour)

meansA B C D E1 18.9 (3) 17.4 (2) 15.3 (1) 21.2 (4) 22.2 (5) 19.0 d

2 18 6 (3) 17 8 (2) 16 4 (1) 21 8 (4) 22 7 (5) 19 4 cd2 18.6 (3) 17.8 (2) 16.4 (1) 21.8 (4) 22.7 (5) 19.4 3 18.5 (2) 19.4 (3) 17.1 (1) 21.5 (4) 23.0 (5) 19.9 bc

4 18.9 (2) 20.8 (3) 16.3 (1) 21.4 (4) 25.1 (5) 20.5 ab( ) ( ) ( ) ( ) ( )5 15.8 (1=) 18.0 (3) 15.8 (1=) 20.6 (4) 23.9 (5) 18.8 d

6 19.3 (2=) 19.3 (2=) 17.4 (1) 22.8 (4) 26.3 (5) 21.0 a

7 16.0 (2) 16.8 (3) 15.3 (1) 20.2 (4) 20.9 (5) 17.8 e

Lot 18.0 c(2)

18.5 c(3)

16.21 d(1)

21.4 b(4)

23.5 a(5)means (2) (3) (1) (4) (5)


Validation of conductivity test for soyabeans:

Method validation report submitted

Following acceptance of report:Rules proposal to be submitted November 2009


3. Research developments

Conductivity (EC) measurements of solute leakage from Brassica species

Mirdad and Powell (2006); Seed Science and Technology, 34: EC predicts germination in artificially aged cauliflower and cabbage

Demir et al (2008); Seed Science and Technology, 3616 commercial cabbage seed lots (2 cvs): EC predicts standard germination and vigour (germination after CD)

Hampton et al (2009); Seed Science and Technology, 37:p ( ) gy26 Brassica seed lots: 3 vegetable species (18 lots), 3 forage species(8 lots) EC correlated with field emergence for all 26 lots and forB. napus (3 forage + 7 vegetable lots)

Matthews et al (2009) submitted12 commercial cabbage lots (1 cv): EC before and after CDgRegression equations between EC before and after CD estimate emergence in modules


• Conductivity alone may identify vigour differences• Conductivity may replace germination after CD to y y p g

predict vigour

Conclusion• Potential for vigour tests for Brassica spp based onPotential for vigour tests for Brassica spp based on

conductivity.• Next step international comparative tests• Next step- international comparative tests


Method ValidationMethod Validation

Controlled Deterioration test for Brassica speciesMethod validation report accepted November 2008p pRules proposal at Annual Meeting 2009

Extension of the Conductivity test to Phaseolus sppMethod Validation report accepted October 2008p pRules proposal at Annual Meeting 2009


Proficiency TestProficiency Test

October 2008: Conductivity test for Pisum sativum

33 laboratories: 13 accredited for vigour

Test preparation: Gillian McLaren, Edinburgh

Thanks to Zita Ripka for supplying seed lots


Distribution of Z-Scores all lots all labs Vigour 08-3 P.sat

Frequency

10

12

14

16q y

4

6

8

10

0

2

-4.5 -4 -3.5 -3 -2.5 -2 -1.5 -1 -0.5 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 More

Z-Scores


Laboratory Ratings

A B C BMP

Laboratory Ratings

Accredited for vigour 10 3 - -

V l t 6 5 3 6Voluntary 6 5 3 6

Voluntary, Accredited 6 2 2 2Voluntary, Non-accredited 0 3 1 4


Training 2008-2009

Seed Moisture, Germination and Vigour WorkshopNakuru, Kenya, November 2008

Gillian McLaren – vigour presentations


The FutureNext year:Rules proposal: Addition of soyabean to species for conductivity test

Validation and Rules proposal: Rate of Germination test for maize

And beyond:Further work on use of conductivity test for Brassica

Direct measurementsTo replace germination after CD

Rate of germination test for other species: comparative tests

Documents

5 VIG Alison & Stan [Kompatibilitätsmodus] · Ms Rasha ElMs Rasha El-Khadem AU ... 12 commercial cabbage lots (1 cv): ... 5 VIG Alison & Stan [Kompatibilitätsmodus] Author: