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5’ dTP-PCR. 3’ dTP-PCR. 85 °C. -dF/dT. 90 °C. -dF/dT. + GM06891 (PM male). Normal male blood spot. + GM07862 (FM male). + NA20244 (NL male, 41 rpts). A. + NA20244 (NL male, 41 rpts). + GM06891 (PM male). + GM07862 (FM male). Normal male blood spot. NA20244 (NL male, 41 rpts). - PowerPoint PPT Presentation
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5’ dTP-PCR
GM04738 (NL male, 30 rpts)
NA20244 (NL male, 41 rpts)
GM06891 (PM male)
GM07862 (FM male)
Normal male blood spot
+ NA20244 (NL male, 41 rpts)
+ GM06891 (PM male)
+ GM07862 (FM male)
70 72 74 76 78 80 82 84 86 88 90 92 94
Temperature (°C)
-dF/dT 85 °C
70 72 74 76 78 80 82 84 86 88 90 92 94
Temperature (°C)
-dF/dT 90 °C
3’ dTP-PCR
GM04738 (NL male, 30 rpts)
NA20244 (NL male, 41 rpts)
GM06891 (PM male)
GM07862 (FM male)
Normal male blood spot
+ NA20244 (NL male, 41 rpts)
+ GM06891 (PM male)
+ GM07862 (FM male)
A
B
Supplemental Figure 5: (A) Melt peaks of blood spots from a normal male mixed with genomic DNA from NA20244 (41 repeat male), GM06891 (premutation male) and GM07862 (full mutation male); (B) Melt peaks from male genomic DNA samples GM04738 (30 repeats), NA20244 (41 repeats), GM06891 (premutation) and GM07862 (full mutation).