PATENT ABSTRACTS

4729951

P R O C E S S F O R T H E I M P R O V E M E N T O F A N T I B I O T I C

P R O D U C T I O N BY IN V I V O G E N E T I C R E C O M B I N A T I O N

Lajos Ferenczy, Antal Mai, Istvan Ott, Gabor Ambrus, Tibor Lang, Szeged, Hungary assigned to Biogal Gyogyszergyar

A process for the production of aminoglycoside antibiotics using fused protoplasts derived from streptomyces. The cells are precultured in a medium containing sucrose, calcium and magnesium salts. Protoplasts are formed and then fused. The fused protoplasts are regenerate and antibiotic producing ability is screened for. The regenerate cells produced an antibiotic com- plex of modified composition or demonstrated increased or reduced antibiotic productivity compared to the fusion partners.

4729954

P L S 0 1 0 P L A S M I D V E C T O R

Sanford A Lacks, Tanjore S Balganesh assigned to The United States of America as represented by the United States Department of Energy

Disclosed is recombinant plasmid pLS 101, con- sisting essentially of a 2.0 Kb maiM gene frag- ment ligated to a 4.4 Kb Tcr DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selec- tion, as well as processes for enrichment of recombinant plasmids in Gram-positive bac- terial systems.

4731325

A R R A Y S O F A L T E R N A T I N G N U C L E I C A C I D F R A G M E N T S

F O R H Y B R I D I Z A T I O N A R R A Y S

Airi Palva, Tuula M Ranki, Hans Soderlund, Helsinki, Finland assigned to Orion-yhtyma

The invention is related to improved nucleic acid reagents comprising arrays of nucleic acid frag- ments and combinations of such fragments. The preparation of such fragments by recombinant

241

DNA techniques and their use in sandwich hybridization methods is also described. By making different combinations of the nucleic acid fragments-some labeled and some affixed to solid carriers, it is possible to create kits for the identification of e.g. veneral diseases. The im- proved nucleic acid reagents comprise two series of at least two alternating nucleic acid fragments, which are homologous to sequences in the nucleic acid to be identified, one of the series being labeled and one affixed to a solid carrier. Nucleic acid fragments belonging to different series must not be homologous to each other. Sandwich hybridization tests performed with arrays of nucleic acid fragments are at least four times as sensitive as sandwich hybridization tests performed with reagents belonging to the prior art.

4731327

M E T H O D F O R S T A B I L I Z I N G R E C O M B I N A N T D N A

M O L E C U L E S A N D E N H A N C I N G T H E E X P R E S S I O N T H E R E O F I N

S T R A I N S O F B A C I L L U S

Yoshi Furutani, Masaru Honjo, Kazuaki Man- abe, Hiroak Shimada, Noboru Tomioka, Miura, Japan assigned to Agency of Industrial Science and Technology; Ministry of International Trade and Indust

A recombinant DNA molecule, a portion of which includes a vector whose replication is in- itiated independently of the temperature- sensitive mutant gene of a temperature-sensitive mutant strain of the genus Bacillus as host is in- troduced into the host. The host is then cultured at a temperature which does not completely in- hibit chromosomal DNA replication. By this process, the recombinant DNA molecule can be stabilized in the host and its expression can be enhanced.

4731499

H Y B R I D C O R N P L A N T A N D S E E D

Vladimir Puskaric, Lori Carrigan, Woodstock, MN, Canada assigned to Pioneer Hi-Bred Inter- national Inc

According to the invention, there is provided a hybrid corn plant, designated 3790, produced by crossing two Pioneer Hi-Bred International, Inc. proprietary inbred lines of corn. This invention

242 PATENT ABSTRACTS

thus relates to the hybrid seed 3790, the hybrid plant produced from the seed, variants, mutants, and modifications of Pioneer hybrid 3790. This hybrid corn plant is characterized by superior yields and excellent early-season cold tolerance, and good grain quality.

4734369

T I S S U E C U L T U R E S O F L Y C O P E R S I C O N S P P .

David A Evans, William R Sharp assigned to Campbell Soup Company

A method is provided of producing variant plant varieties of Lycopersicon spp. by tissue culture techniques employing 6-benzyladenine at high concentrations. The technique produces phenotypic variants that are the result of heritable nuclear genetic changes.

4734363

L A R G E S C A L E P R O D U C T I O N O F DNA P R O B E S

Nanibhushan Dattagupta, Peter M M Rae, Donald M Crothers, Thomas R Barnett assigned to Molecular Diagnostics lnc

A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said poly- nucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized poly- nucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the ex- tended oligonucleotide. The product can be used for making analytical probes.

4734491

DNA S E Q U E N C E S E N C O D I N G HYBRID L Y M P H O B L A S T O I D -

L E U K O C Y T E H U M A N I N T E R F E R O N S

Marvin H Caruthers assigned to University Pa- tents Inc

DNA sequences encoding for novel human lymphoblastoid-leukocyte hybrid interferons. Methods of making nucleotide sequences com- prising ribosome binding sites, promoter re- gions, and segments of human lymphoblastoid and leukocyte interferons are described. DNA sequences for hybrid interferons are cloned and expressed in plasmid vectors. Transformed microorganisms and novel lymphoblastoid- leukocyte polypeptide interferons having bio- logical or immunological acitivity are disclosed.