4 Ctkc Bp Vitekms Simplified User Guide en 2012mar08

8/10/2019 4 Ctkc Bp Vitekms Simplified User Guide en 2012mar08

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S im p l i f i e d U s e r u i d e

V I T EK MS Sim p l i f ie d U s e r G u id e V e rs i o n 4 p a g e 1


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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 2

Table of contentsVITEK MS instrument

Configuration...

Workflow

Material and consumables ..

Protocol ID&AST..

Protocol ID

Protocol AST. page 10

page 4

page 5

page 6

page 7

page 8

page 9


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VITEK MS Prep Station page 11

MYLA Slides composition.... page 12

VITEK MS Acquisition Station. pages 13-15

MYLA : Results to be reviewed... pages 16-19

MYLA : Results to be approved.. pages 20-21

MYLA : Electronic Signature page 22

MYLA : AST review..... page 23

VITEK MS Users maintenance... page 24

Table of contents


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VITEK MS Instrument

Indicates an alarm

Status OK


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MS-ID Database

( CE/IVD) embedded into MylaTM

P ow er I on is in g V ac uu m N eg at iv eP ow er I on is in g V ac uu m N eg at iv eP ow er I on is in g V ac uu m N eg at iv e

Po w e r Io n is in g V ac uu m N eg at iv ePo w e r Io n is in g V ac uu m N eg at iv ePo w e r Io n is in g V ac uu m N eg at iv e

Prep Station

Configuration


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Workflow

Acquisition Station

P ow er Io ni si ng V ac uu m Neg at iv eP ow er Io ni si ng V ac uu m Neg at iv eP ow er Io ni si ng V ac uu m Neg at iv e

Prep Station

Slidedescription

Spectra

Slidedescription

Peak lists

Slide

description

MS-ID Database

( CE/IVD) embedded into MylaTM

Step 1Operator

Information

Step 3

Step 2


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Material & consumables

For Bacteria & Fungi

Pipette ( 0.1-2.5l) + tips without filter

Loops 1l

VITEK MS-DS Target Slides 48 spots ref 410893

Calibrant : LyfoCults Plus E.coli ATCC 8739 Ref. 301145

Columbia agar + 5 % Sheep blood Ref. 43 041 / 43 049

or Trypcase Soy agar + 5 % Sheep blood Ref. 43 001 / 43 009

Matrix: VITEK MS-CHCA ref 411071

For Molds & Mycoplasma

Please refer to :

For Yeast

VITEK MS-FA ref 411072


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ProtocolIdentification VITEK MS

& AST VITEK 2Prep Station

Matrix deposit

1L CHCA *

Calibrant

Deposit

+ Matrix

Step 2

Step 4

A B

Step3

McF

* Prior extraction for yeasts only: 0,5L Formic acid deposit

Step 1


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ProtocolIdentification VITEK MS only

Prep Station

Matrix deposit

1L CHCA *

Calibrant

Deposit

+ Matrix

Step 2

Step 3


O

therisolates

OtherAcquisitiongroups

Step 1


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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 0

ProtocolAST VITEK 2 only

Prep Station

McF



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6 .Scan the

VITEK2 Card

barcode andplace the card

on the cassette

1 . Scan the

target slide

2 . Scan the Lab

ID on the plate

3 . Choose

between

Bacteria or Fungi

4 . Deposit the

spots

5 . Prepare thebacterial McF

suspension

7 . F4 Validate

Prep Station


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Slides Composition

Homepage


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Acquisition Station

1. Open the door

2. Scan the

target slides

barecodes

3. VITEK MS

retrieves target

slides

information

4. Load the

target slides into

the VITEK MS

5. Start the

Acquisition

6. Acquisition in

progress

7. End of

acquisition


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Acquisition Station:Spots color code


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Acquisition Station:Spectra features

Average of all the spectra

collected for the sample

Averaged data afterprocessing

(smoothing, threshold

substraction, peak

detection)

Peak list sent to the MS-ID

server


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Results to be reviewed

Detail of VITEK MS ID to be

reviewed

Homepage

Technologist Dashboard


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Refresh the list of results

Review selected identif ication results.

The results are removed from this list

and will be displayed in the Reviewed tab

Review all identif ication results with

a high confidence level.



Discard selected identification results.



Enter and assign a comment for theselected result (s) if applicable.

Print a report file of the selected

result(s) to be reviewed


Indicates that an organism is a highly pathogenicorganism or a critical pathogen

This result must be review using the following icon


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Rules for Consolidation :

-The consolidated identification result can be sent as it is.

- Consolidate the identification by selecting a result

- Result cannot be consolidated, repeat the analysis

(1) Select the

organism name(2) Validate the selected

organism name

Optional: Display a pop-up

window to enter comments

for the selected result(s)

Indicates that an organism has been selected


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Results to be approvedOnly if the 'Review and Approve for result

Validation' setting is enabled in MYLA

Detail of VITEK MS ID to be

approved

Homepage

ManagerDashboard


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Refresh the list of results

Approve the selected result (s)

The results are removed from this list andwill be displayed in the Approved tab

Approve all ident ification resul ts with

a high confidence level


and will be displayed in the Approved tab

Reject the selected result(s)

The rejected results are removed from this

list and will be displayed in the To be

reviewed tab with the status To rework

Display a pop-up window to enter

comments for the selected result(s)

Print a report file of the selected

result(s) to be approved

Results to be approvedOnly if the 'Review and Approve for result

Validation' setting is enabled in MYLA

When a comment is entered, the button

is displayed on the result line


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Electronic SignatureOnly if the Electronic Signature

option setting is enabled in MYLA

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When you review, discard, approve & reject result(s), an Electronic Signature

has to be entered to confirm the sending of results.

The 1st time, you have to enter the User name and Password, then only thePassword is required.


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AST Review


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VITEKMS Users maintenance

Maintenance Operation Frequency Refer to

Cleaning the VITEK MS

and the Acquisition Station

+ Acquisition Station monitor

To be established according your

internal procedure .

To be written in your SOP

Chapter 5 Maintenance

Cleaning the Adapter To be established according yourinternal procedure .


Changing the desiccant When the desiccant turns to pink

Cleaning of the O-ring seal & thesealing face of the door To be established according your

internal procedure .


Chapter 12

Documents

4 Ctkc Bp Vitekms Simplified User Guide en 2012mar08