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#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Warm upWarm up
How did we use indicators in the Diffusion Lab?
How did we use indicators in the Diffusion Lab?
Find your Diffusion Lab
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through a MembraneDiffusion Through a Membrane
DIN – Turn your lab over to the back. Write down a few sentences about what is
happening in the video AND why it is happening.
EGG VIDEO
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Review of the Cell MembraneReview of the Cell Membrane
• The Cell Membrane is Semi-Permeable or Selectively Permeable
• Small molecules (like glucose) are able to pass through the membrane while large molecules (like starch) cannot pass through
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through A MembraneDiffusion Through A Membrane
glucose molecule
PART of a starchmolecule
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
What we used for the labWhat we used for the lab
• Dialysis Tubing – used to make a “cell” that will allow small particles to pass through and not large particles
• Glucose Solution – a simple sugar made of one small molecule of sugar
• Starch Solution – a complex carbohydrate made of hundreds of simple sugars bonded together
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
IndicatorsIndicators
• Starch Indicator Solution – (Iodine), a really small molecule (It’s only 2 atoms big)
• Glucose Indicator Solution – Benedict’s solution
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Initial CellInitial Cell
“Cell” with glucose and starch
Water and starch indicator solution
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
The Final CellThe Final Cell
The water stayed amber
The cell turned blue-black
Water from outside the cell tested positive for glucose
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Quick Video of Iodine (Starch Indicator
Solution) diffusing INTO the Cell!
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
What Happened??What Happened??
• Glucose was small enough to pass through the membrane so it ended up in the water outside the cell
• Starch was too big to pass through the membrane so it didn’t get out of the cell
• Iodine (starch indicator solution) was small enough to pass through the membrane so it ended up in the cell turning the cell blue-black
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through a MembraneDiffusion Through a Membrane
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through a MembraneDiffusion Through a Membrane
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
RED ONION IN FRESH WATERRED ONION IN FRESH WATER
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Red Onion in Fresh WaterRed Onion in Fresh Water
cell wall
cytoplasm
red onion cellsin tap watercell membrane
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Add Salt Water to CellsAdd Salt Water to Cells
bathing the cells in NaCl = salt
water, by “wicking”it through
NaCl
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
RED ONION IN SALT WATERRED ONION IN SALT WATER
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through a MembraneDiffusion Through a Membrane
red onion cells insalt water
cytoplasm
cell wallcell membrane
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through A MembraneDiffusion Through A Membrane
bathing the cells in distilled water, by “wicking” it
through
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through a MembraneDiffusion Through a Membrane
cell wall
cytoplasm
red onion cellsin distilled water – returned to normal
cell membrane
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through A MembraneDiffusion Through A Membrane
Which cell is in distilled water andwhich is in salt water?
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Diffusion Through A MembraneDiffusion Through A Membrane
Applications – salt on roads to melt snow intravenous saline solutions salty foods make you thirsty salt on slugs to kill them salty foods do not spoil as easily gargling with salt water digestion of starch to glucose
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
• Quick video of what happened to iodine in “cell” tube
• A diffusion lab for absent students
• Student summary of diffusion state lab
• Osmosis demo with raw egg
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Activity – Analysis Questions
• Your table will be assigned one question from the analysis section on pages 8 and 9
• Work on the question with your table for 5 minutes and put your answer on your easel.
• Be ready to share and explain to your classmates
** If you finish early, check that ALL other sections of the lab are complete
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Answer on looseleaf and attach to lab
1. How did we observe diffusion and osmosis in this lab activity? Did this help with your understanding of these concepts? Explain.
2. Why was lab safety important during this activity?
3. What was your favorite part of this lab? What was most challenging?
Answer on looseleaf and attach to lab
1. How did we observe diffusion and osmosis in this lab activity? Did this help with your understanding of these concepts? Explain.
2. Why was lab safety important during this activity?
3. What was your favorite part of this lab? What was most challenging?
REGENTS PRACTICEReflection Reflection
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
#30 How did we observe diffusion in
the State Lab?
Ticket out the DoorTicket out the Door
Think of our experiment yesterday…
1.What molecule diffused OUT of the “cell”? Why was it able to?
2.What molecule could NOT diffuse out of the “cell”? Why not?
3.What molecule diffused INTO the cell? What did it react with in the cell? How could you tell it reacted?
