250150100

75

50

37

25

20

15

10

Figure 1

’

-Conglycinin

Acidic

Basic

Glycinin

Mr

1 2 3

x105

0 5 10 15 20 25 30 Time(min)

0.0

0.5

1.0

1.5

Intens.

2.0 1:5

1:25

1:50

0.0

0.5

1.0

1.5

x105

0 5 10 15 20 25 30 Time(min)

Intens.

1:25

1:50

A

B

Figure 2

Figure 3

0

1

2

x105Intens.

0 5 10 15 20 25 30 Time(min)

A

BC

D

E

FG H

A

B

C

D

EF

G H

A

B

CD

EF

G

H

A

B

C

DE

FG

H

3

4

5

6

7

8 A

B

C

D

9

Figure 4

0.0

2.5

5.0

x105Intens.

0 5 10 15 20 25 30 Time(min)

A

B

C

D

E

F

G H

Base peak electropherogram

Figure 5

Mr (3)640.3

0.0

0.5

1.0

Intens.

200 300 400 500 600 700 800 900 m/z

Mr (2)630.4

Mr (4)684.3

M+H417.2570

M+H641.3511 Mass spectra

(9.5 min, peak A)

0

1

2

3

4

5

Intens.

200 300 400 500 600 700 800 900 m/z

M+H685.3153

Deconvolutedmass spectra

(9.5 min, peak A)

x105x104

M+5H587.4964

M+4H734.1155

0.0

0.5

1.0

1.5

2.0

2.5

Intens.

400 600 800 1000 1200 1400 1600 m/z

Mass spectra(15.2 min, peak C)

x105

0

2

4

6

500 1000 1500 2000 2500 3000m/z

Deconvolutedmass spectra

(15.2 min, peak C)

0.0

0.5

1.0

1.5

1000 1500 2000 2500 m/z

Mr (1)933.1

Mr (3)2754.3

Mr (2)2666.2

Mr (4)2776.3

Mr (5)2792.2

0.0

0.2

0.4

0.6

0.8

Intens.

M+2H1378.6605

500 1000 1500m/z

Mass spectra(25.7 min, peak G)

Deconvolutedmass spectra

(25.7 min, peak G)

M+H631.3779

Mr (1)416.2

M+3H978.4777

M+1H658.3186

Mr (1)657.3

Mr (2)2931.4

M+3H919.4486

x105Intens.x105

Intens.x105

C

A

B

Figure 6

5 10 15 20 25 300

Time(min)

5 10 15 20 25 300

Time(min)

0

1

2

3

4

x105Intens.

Rel

ati

ve

inte

ns

ity

Base peak electropherogram

Extracted ion electropherograms

Figure 7

0.0

1.5

3.0

Intens.x105

4.5

6.0

Time(min)

0 5 10 15 20 25 30

7.5

Conventional soybean

Transgenic soybean