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Also available with Zivak® Multitasker Full Automated Sample Preparation AND Injection System
1.7 minutes analysis time
Sensitive separation with Zivak® 25-OH Vitamin DUHPLC column
Approximately 1000 analysis with one column
Suitable for anyLC-MS/MS System
25-OH Vitamin D2/D3LC-MS/MS Analysis Kitin Human Serum Samples
VITAMIN PROFILING
Main methods and procedures that have beenselected are based on EN ISO 13485 and 98/79/EC.
1.7 MINUTES
LC-MS/MS analysiswith high accuracy
and quantitativedetection
Properties of the analysis kitAccuracy 96 %
!
*Reference upon request
[email protected]+90 212 210 62 15 +90 212 210 62 14
ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY
Distribuido en España por: INGENIERIA ANALITICA, S.L Tel: (+34) 902.45.6677Fax: (+34) 902.46.6677 www.ingenieria-analitica.com [email protected]
Sample Chromatograms and Validation Results
25-OH Vitamin D2/D3LC-MS/MS Analysis Kitin Human Serum Samples
The test results have been validated with an AB Sciex API 4000 LC-MS/MS instrument in Nov. 2014.
Tested by with an accuracy of 96%
Sample chromatograms are for serum calibrator of Zivak® 25-OH Vitamin D2/D3 LC-MS/MS Analysis Kit
(µg/L)
(µg/L)
(%)
(%CV)
(%CV)
( 0-200 µg/L)
25-OH Vitamin D3
LOD
LOQ
Accuracy
Precision Intra-Assay
Precision Inter-Assay
Linearity (R2)
0.51
1.53
98.5
3.4
4.4
0.998
(µg/L)
(µg/L)
(%)
(%CV)
(%CV)
( 0-200 µg/L)
25-OH Vitamin D2
LOD
LOQ
Accuracy
Precision Intra-Assay
Precision Inter-Assay
Linearity (R2)
0.50
1.50
97.6
2.6
3.5
0.998
0.25 0.50 0.75 1.00 1.25 1.50 1.75
minutes
0
1
2
3
4
5
6383.3>257.2 [-16V]VITAMIN D3 1.06
0.25 0.50 0.75 1.00 1.25 1.50 1.75
minutes
0
1
2
3
4395.2>269.2 [-18V]VITAMIN D2 1.11
VITAMIN PROFILING
Distribuido en España por: INGENIERIA ANALITICA, S.L Tel: (+34) 902.45.6677 www.ingenieria-analitica.com [email protected]
