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2424 Evaporative Light Scattering DetectorPutting confidence in your detection strategy
Benefits of adding ELS detection ■■ Orthogonal detection
technique
■■ Detection of analytes
without UV/Vis
chromophore and/or do
not ionize well in MS
■■ Nanogram-level
detection limits
■■ Mass response
independent of
analyte’s optical
properties
Waters 2424 ELS Detector.
The 2424 ELS Detector allows you to reproducibly detect semi-volatile components as demonstrated by this chromatographic test mix of semi-volatile and non-volatile components. Excellent detection is achieved across the range of components without interference from the DMSO sample diluent.
Caffe
ine
Naph
azol
ine
Oxy
met
azol
ine
Oxy
buty
nin
Nabu
met
one
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ACHIEV E RICHER ANALYSIS
The Waters® 2424 Evaporative Light Scattering (ELS) Detector, with its
compact and innovative design, offers near universal detection of non-
volatile and semi-volatile sample components. The 2424 ELS Detector is
compatible with both isocratic and gradient separations and has nebulizer
options to cover a wide range of analytical and microbore flow rates. By adding
the orthogonality of ELS to your detection strategy, you increase peak detection
confidence across a wide application space. The 2424 ELS Detector can be
used as part of an Alliance® HPLC or LC/MS system under the control of either
Empower® or MassLynx® software, or can be operated as a stand-alone detector.
Quantification for your entire sample
Finding the right balance in a detector designed for evaporative light scattering
detection can be the difference between good sensitivity of semi-volatile
components and high peak dispersion. The 2424 ELS Detector was designed
with this critical balance in mind and delivers reproducible quantification for
semi-volatile components without adding high dispersion to fast LC separations.
Sample components that elute in the column void volume can be difficult to
detect with many techniques, creating an issue of particular concern for strong
UV absorbing diluents such as DMSO. The desolvation characteristics of the
2424 ELS Detector can remove the sample diluent interference to give visibility
to unretained components in your sample.
AP PLICAT ION SOLUT IONS FOR T HE LABORATORY
Laboratories around the world rely on the proven performance
of Waters ELS detectors for superior results across a vast range
of applications, including the analysis of carbohydrates, fatty
acids, lipids, surfactants, and pharmaceuticals.
Complex applications with gradient analysis
Whether trying to monitor a complex process such as the
consistent production of food oils or verifying their authenticity
to prevent adulteration, the use of gradient analysis is
important for providing a wider separation range of your
samples. The 2424 ELS Detector is entirely compatible with
gradient analysis, and with significantly reduced stabilization
times as compared to other ELS detectors, you can get
consistent answers, faster.
Enhance your existing detection capabilities
For applications where not all components have a UV
chromophore, adding ELS detection expands the visibility
of your sample by providing a complementary
detection capability.
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Molasses
Food sugar standard1 2 3
45
12
31. p-Toluamide 2. Fructose 3. Glucose4. Sucrose 5. Maltose 6. Lactose
The gradient analysis of triglycerides in edible oils, overlay of safflower oil (blue) and olive oil (green).
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sulfa
me-
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lfam
e-K
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harin
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harin
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amat
e
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alos
e
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arta
ne
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espe
ridin
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arta
me
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espe
ridin
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0.000.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00
AU
LSU
While some artificial sweeteners can be analyzed by UV, several lack UV chromophores. In this example, all these artificial sweeteners can be quantified by adding ELS detection.
Simple food sugars lack a UV chromophore, but they are easily detected and quantified in complex matrices using the 2424 ELS Detector.
ALL T HIS T ECHNOLOGY, WIT HOUT ALL T HE BENCHSPAC E
The 2424 ELS Detector hosts a multitude of innovative design features for detection of your non-UV samples, but with
simple integration into your system and your laboratory environment.
Designed for your laboratory■■ Compact footprint for system stacking and reduced
laboratory space requirement
■■ Front-mounted, user-installable lamp
■■ Easily accessible nebulizers covering wide flow rate range
■■ Open access ready with accelerated nebulizer stabilization
Maximize your method performance■■ Sampling rates up to 80 points/sec
■■ Independent optimization of digital filtering
■■ Active nebulizer heating and cooling
■■ Auto-optimize gain function
■■ Optional external column heater control
Detection technology■ Reference energy monitoring for consistent long-term signal■ 60° scatter angle detection to minimize stray light and optics polarization
for long-term optimal sensitivity■ Normalized energy output for improved bench-to-bench repeatability
Nebulization technology■ Concentric flow nebulizer designed
for consistent droplet formation■ Heating for method optimization■ Cooling for rapid stabilization
Desolvation technology■ Temperature controlled and
monitored to provide optimal conditions for desolvating semi-volatile components
■ Optimized geometry for analytical HPLC peak volumes and flow rates
■ Desolvation of interfering sample diluent peaks to expose unretained sample components
Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com
Waters, Empower, MassLynx, and Alliance are registered trademarks of Waters Corporation. SystemsQT and T he Science of What’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.
©2013 Waters Corporation. Printed in the U.S.A.February 2013 720004525EN TC-PC
COMPLET E T HE CHROMATOGRAPHIC P ICTURE AND BUILD HIGHER CONFIDENC E IN YOUR RESULT S
Pair a 2424 ELS Detector with a Waters 2489 UV/Vis Detector and SQ Detector 2
single quadrupole mass detector, and achieve the capability to:
■■ Derive more complete peak information in one chromatographic run,
■■ Detect molecules that lack a UV/Vis chromophore, complementing absorbance
detector information, and
■■ Analyze compounds that don’t ionize well by MS.
Supported in both Empower and Masslynx software, you can select the right
chromatographic data system for your laboratory’s needs. The 2424 ELS Detector
is also compliance-ready as part of the Alliance HPLC family of systems supported
within the Systems Qualification Tool (SystemsQT™) technology.
Alliance HPLC System with 2424 ELS Detector, 2998 PDA Detector, and SQ Detector 2.
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