2242 Strata Br - Chromservis Strata Full... · The Strata line is comprised of ultra-pure sorbents, which are designed to give high recoveries for traditional SPE applications such

SM SM

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Why Strata? 2 Revolutionary Sorbents 3 strata-X 3

strata-X-C 7

Screening Drugs of Abuse 11 Strata Screen-C 11

Strata Screen-A 11

Strata Screen-C GF 11

Method Development 13

Phase Descriptions 15 Reversed Phases 15

Normal Phases 16

Ion Exchange Phases 17

Ordering Information 18 Strata SPE Tubes 18

Large Format SPE 19

High-Throughput SPE 20 96-Well Plates 20

Bulk SPE 21

Sepra 21

Sample Processing Stations 22

SPE Accessories 22

Table of contents

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Phenomenex technical consultants are available to help with your SPE method development. Please feel free to contact us or visit www.phenomenex.comfor SPE:

n Troubleshooting

n Technical Solutions

n Converting Liquid/LiquidExtraction to SPE

n Method Optimization

Tel: 310-212-0555 Fax: 310-328-7768 Web: www.phenomenex.com

2

Why Strata™?

If Strata SPE or Sepra Bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE products or Sepra Bulk for FREE!

Rugged The unique manufacturing process makes sorbents extremely rugged and

provides consistently high recoveries for a wide range of applications.

ReproducibleSophisticated characterization techniques are used to ensure high

efficiencies and invariable masses from lot-to-lot resulting in highly

reproducible sorbents.

RevolutionaryThe Strata line is comprised of ultra-pure sorbents, which are designed

to give high recoveries for traditional SPE applications such as Strata

C18, and revolutionary, deconditioning resistant, sorbents that streamline

the method development process and redefine the limits of SPE such

as strata-X and strata-X-C.

GuaranteedA comprehensive certificate of analysis is included in every box. QC data

obtained throughout the production process is documented to provide

complete traceability and ensure guaranteed quality.

What More Could You Ask For?


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P

P

P

P

P

Feature strata-X C18Styrene-divinylbenzene

polymer

Surface Chemistry

Surface modified styrene-divinylbenzene polymer

Silica particle functionalized with C18 Highly crosslinked polymer

Retention Mechanism

Hydrophobic, H-bonding, andAromatic

Hydrophobic Hydrophobic and Aromatic

Hydrogen donating characteristics

Slightly acidic surface, shows a strong interaction with basic compounds

None None

Resistant to deconditioning

Yes, can run dry without negatively impacting retention

No No

Water-wettable Yes No No

Analyte Capacity 10-15% of sorbent bed mass 1-5% of sorbent bed mass 10-15% of sorbent bed mass

VersatilityEnhanced polar analyte retention compliments ability to extract hydrophobic compounds

Extracting hydrophobic compounds

Extracting hydrophobic compounds

Polymeric Reversed Phase Sorbent for Extraction of Acidic, Basic and Neutral Compounds

strata-X is a patent pending versatile sorbent that can be used to separate both polar and non-polar compounds. This revolutionary technology consists of hydrophilic, hydrophobic, and aromatic retention mechanisms that offer the following benefits:

• Strong π−π retention mechanism that provides high recoveries for aromatic compounds

• Deconditioning resistant sorbent bed that can run dry without losing selectivity

• Enhanced polar selectivity for polar metabolites

X-SPEct More from strata-X strata-X vs. Traditional SPE Sorbents.

Improved Recovery for Aromatics

strata-X is a water-wettable, surface modified styrene-divinylbenzene polymer which retains analytes by π−π interaction, giving high recoveries for aromatic compounds. Elution solvents that have strong π character such as acetonitrile can be used to disrupt the π−π interaction of the sorbent and analyte.

strata-X Ordering Information: p. 6

π electron density of aromatic

analyte

π electron density of strata-X

Sorbent Characteristics

Particle Size 33µm

Pore Size 85Å

Surface Area 800m2/g

pH stability 1-14


Put A Stop To Stopcocks

The deconditioning resistant sorbent bed eliminates the need to adjust stop-cocks on a vacuum manifold during manual tube processing, and eliminates the well–to-well variability that often results from 96-well plate automation.

Maintains High Recovery Even After Vacuum Dried for 10 minutes!

The strata-X Method for Reversed Phase ExtractionVolumes suggested are for a 30mg bed mass.

4

CH3OHCH3OH

Analyte elutes!

5% CH3OH + H2O5% CH3OH + H2O

Impurities eluteoff the sorbent!

Analyte

Impurities

H2O + CH3OHH2O + CH3OH

H2OCH3OHCH3OH CH3OHCH3OH

Analyte elutes!



Analyte

Impurities



Analyte elutes!



Analyte

Impurities



Analyte elutes!



Analyte

Impurities


H2OCH3OHCH3OH

DoxepinRanitidine

CaffeineTheophylline Procainamide

Silica-basedC18

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Conditions:Tubes: 30mg/1mL strata-X, 100mg/1mL Strata C18-E

Sample: Analytes spiked in phosphate buffer saline (PBS) solution at concentration of 1µg/mL, n=3

Method: The strata-X method was used. After the conditioning step the sorbent was dried under vacuum up to 10 min. Percent recoveries were determined by HPLC analysis.

Conditions:Method: Analysis spiked in porcine serum at concentration of 0.5 or 1.0µg/mL. The strata-X method was used, see above. Contact Phenomenex for more complete method details.

1.Condition

with 1mL methanol

2.Equilibratewith 1mL water

3.Load Sample

4.Wash Impuritieswith 1mL 5% methanol

in water and dry 30 sec. at full vacuum

5.Elute Analyte!with 1mL methanol

or 50:50 acetonitrile/ methanol

Relative Recovery of Acidic, Basic, and Neutral Compounds

CH3OHCH3OH

Analyte elutes!



Analyte

Impurities


H2OCH3OHCH3OH


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strata-X Desalting Method

Polarity Scale Log P* Compounds % Recovery

PolarPolarPolarPolar

-0.78-0.07-0.020.46

TheobromineCaffeine

TheophyllineAcetaminophen

99969896

Moderately PolarModerately Polar

1.462.15

Phenol2-Chlorophenol

9597

Non-PolarNon-Polar

4.134.29

2,4,6-TribromophenolDoxepin

10193

Flexibility for a Wide Range of Applications

The surface chemistry of the strata-X sorbent allows for very selective interactions between the sorbent surface and analyte, resulting in versatility for a wide range of applications, as illustrated in the example below.

Selective Elution and Desalting of Peptides

Enhanced Polar Selectivity Great for the extraction of polar metabolites and non-polar parent compounds!

*Log P is the partition coefficient that can be used to determine the polarity of a compound.

An example of the compounds that can be retained by strata-X!

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OH

BrBr

Br

OH

OH

Cl

O

N

Phenol

2-Chlorophenol

Doxepin Caffeine Theophylline

2,4,6-TribromophenolTheobromine

NO

NN

O

N

NH

N

O

N

NO

N

N

O

NO

NH


6

1mL (100/box) 3mL (50/box) 6mL (30/box)

Phase 30mg 60mg 200mg 500mg 100mg 200mg 500mg

strata-X 8B-S100-TAK 8B-S100-UBJ 8B-S100-FBJ 8B-S100-HBJ 8B-S100-ECH 8B-S100-FCH 8B-S100-HCH

Note: tube sizes not proportional nor actual size.

• Extraction of Phenols from Water using

strata-X (TN-001)

• Extraction of Nitroanilines from Water

using strata-X (TN-003)

• Extraction of Acidic, Basic, and Neutral

Compounds using strata-X (TN-004)

• TCAs Extracted from Serum using

strata-X (TN-006)

• Fast Extraction of Acyclovir from Plasma

using strata-X On-line Cartridge (TN-009)

Ordering Information

strata-X Tubes

strata-X 96-Well plates (2/Box)

Phase 5mg 10mg 30mg 60mg

strata-X 8E-S100-NGB 8C-S100-AGB 8E-S100-TGB 8E-S100-UGB

strata-X On-Line Extraction Cartridge

Order No. Dimensions Unit/Box Price

00M-S033-B0-CB strata-X on-line extraction cartridge, 25µm, 20 x 20mm

ea

CH0-5845 Cartridge holder, 20mm ea

Technical Notes Available Contact your Phenomenex Distributor and Request A Technical Note Today.


Feature strata-X-C Silica-Based Mixed-Mode

Surface Chemistry

Styrene-divinylbenzene polymer surfaced modified with a strong cation exchanger group

Functionalized silica particles (Alkyl chain + SCX)

Retention Mechanism

Hydrophobic, H-bonding, Aromatic and Ionic

Hydrophobic and Ionic

Resistant to deconditioning

Yes, can run dry without negatively impacting retention

No

pH Stability 1-14 2-7.5

Versatility

Multimode retention mechanism gives enhanced recoveries for all 1°, 2°, and 3° amines including those with aromatic and polar character

Traditionally used for 1°, 2°, and 3° amines

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New Phase

Polymeric Cation Exchange Sorbent for Extraction of Basic Compounds

strata X-C is a patent pending sorbent that gives extremely high recoveries for bases using the strong interaction of ion exchange. The revolutionary selectivity of strata X-C offers significant advantages over silica-based cation exchange sorbents:

• Mixed-mode functionality includes π−π selectivity for aromatic bases and cation exchange selectivity for primary, secondary and tertiary amines

• Strong secondary interactions eliminate the use of

halogenated solvents when cleaning up drugs of abuse

• Deconditioning resistant sorbent that can run dry without losing ion exchange selectivity

• Ability to fractionate acidic and neutral components from bases

• 100% organic washes provide cleaner extracts from biological samples reducing ion suppression

X-SPEct More from strata-X-C strata-X-C vs. Silica-Based Mixed-Mode Sorbents.

Sorbent Characteristics

Particle Size 33µm

Pore Size 85Å

Surface Area 800m2/g

pH Stability 1-14

Ionic Capacity 1meq/g

strata-X-C Ordering Information: p. 10Tel: 310-212-0555 Fax: 310-328-7768 Web: www.phenomenex.com

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New Phase

strata-X-C Method for THC

*After elution samples are reconstituted and derivatized, then analyzed on a GC/MS using a Zebron™ ZB-5. Contact Phenomenex for additional details about this method.

strata-X-C Ordering Information: p. 10

Strong Secondary Interactions Eliminate the Use of Halogenated SolventsSpecialized Extraction of THC from Urine on strata-X-C.

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THC-COOHTHC-COOH Glucuronide

(Base Hydrolysis)

Acrylamide

Verapamil

Doxepin

Naproxen

Diphenhydramine

Chlorpheniramine

Diazepam

Flunitrazepam

Flurazepam

Bromazepam

Pseudoephedrine

Phenylpropanolamine

Acetaminophen

Codeine

Metoprolol

Propranolol

Phencyclidine

Methadone

Methamphetamine

Neurotoxin

Antidepressant

TCA

NSAID

Antihistamine

Benzodiazepine

Decongestant

Analgesic

Beta Blockers

Drugs of Abuse

0 20 40 60 80 100

% Recovery

% Recovery for a Wide Range of Analytes on

strata-X-C

High Recoveries for Basic Compounds

1 Conditionwith 2mL methanol

2 Equilibratewith 2mL 0.1N HCl

3 LoadHydrolyzed samples

4 Wash 12mL 0.1N HCl

5Wash 2

2mL 0.1N HCl/acetonitrile 70:30* dry for 4 minutes

6Wash 3

200µL glacial acetic acid, let it percolate

under gravity 30-40 sec dry 1min

7 Elute2mL 2% glacial acetic acid in acetonitrile

5% NH4OH+ CH3OH5% NH4OH+ CH3OH

Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH


Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH


Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH


Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH

CH3COOHCH3COOH

Analyte elutes!

0.1N HCL0.1N HCL


0.1N HCL +ACN0.1N HCL +ACN


CH3COOH +ACNCH3COOH +ACN

Impurities elute!

Analyte

Impurities

0.1N HCLCH3OHCH3OH

CH3COOHCH3COOH

Analyte elutes!

0.1N HCL0.1N HCL





Impurities elute!

Analyte

Impurities

0.1N HCLCH3OHCH3OH

CH3COOHCH3COOH

Analyte elutes!

0.1N HCL0.1N HCL





Impurities elute!

Analyte

Impurities

0.1N HCLCH3OHCH3OH

Class of Compounds

The mixed-mode functionality and wide pH range of this sorbent make strata-X-C extremely versatile for toxicological and phar-maceutical analyses.


New Phase

9

Ion suppression study performed with post column infusion of Atenolol, positive ESI mode m/z 267. Contact Phenomenex for additional method details.

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strata-X-C Ordering Information: p. 10

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SPE Conditions: Tubes: 60mg/3mLMethod: The IS (5.0µg/mL) for acidic and neutral drugs was spiked with analytes in PBS before loading onto the tube. The ext. std. (50.0µg/mL) for basic drugs was added to elution 2 before evaporation.

HPLC Conditions: Luna® C18(2) Column was used. Contact Phenomenex for details.

Complete Removal of Biological Contaminants The strong ion exchange mechanism allows for aggressive washes with 100% organic solvents with no loss in recovery for analytes. This allows for the complete removal of components, which may cause ion suppression on a MS.

Reduce Ion Suppression with strata-X-C

Selective Recovery of Bases from Acidic and Neutral Components

The revolutionary mixed-mode selectivity of strata-X-C may be used to fractionate basic compounds from acidic and neutral components. This is especially useful for applications that may require selective elution of acidic, neutral and basic compounds.

Fractionation on strata-X-C

The strata-X-C MethodVolumes suggested are for a 60mg bed mass.


Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH

*Go Slow; Flow rate not to exceed 2 mL/min. The extract of this method is volatile and can be lost during evaporation.

1.Condition

with 2mL methanol

2.Equilibratewith 2mL water

3.Load Sample

acidified with 20µL H3PO4/mL

4.Wash Impuritieswith 2mL 0.1% H3PO4

and dry 1-3min*

5.Removal of

Neutral & Acidic Components

with 2mL methanol

6.Elute Analyte!

with 2mL 5% NH4OH/methanol


Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH 5% NH4OH+ CH3OH5% NH4OH+ CH3OH

Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH

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Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities



Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities



Analyte elutes!

0.1% H3PO40.1% H3PO4


CH3OHCH3OH


Analyte

Impurities


H2OCH3OHCH3OH


10


• Rapid Removal of Acrylamide from French

Fries using strata-X-C (TN-007)

• Basic Drugs from Biological Matrices on

strata-X-C (TN-008)

• Extraction of Sulfa Drugs from Plasma

using strata-X-C (TN-010)


strata-X-C Tubes

strata-X-C 96-Well plates (2/Box)


Phase 30mg 60mg 200mg 500mg 100mg 200mg 500mg

strata-X-C 8B-S029-TAK 8B-S029-UBJ 8B-S029-FBJ 8B-S029-HBJ 8B-S029-ECH 8B-S029-FCH 8B-S029-HCH

Phase 10mg 30mg 60mg

strata-X-C 8E-S029-AGB 8E-S029-TGB 8E-S029-UGB

New Phase



THC-COOH Recovery Comparison Cocaine Metabolite Recovery Comparison

Screen C is a great alternative to Varian Bond Elut Certify®, ISOLUTE® Confirm™ HCX, J.T. Baker narc-2™, Worldwide Monitoring® Clean Screen™ DAU*

Screen A is a great alternative to Varian Bond Elut Certify® II, ISOLUTE® Confirm™ HAX, J.T. Baker narc-1™, Worldwide Monitoring® Clean Screen™ THC*

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Figure 2. Percent absolute recovery of a wide spectrum of analytes by Strata™ Screen-C GF. Contact Phenomenex for details concerning this method.

Figure 1. Average flow time of porcine serum (diluted 1:1 with PBS buffer) under gravity flow. Flow rate of Strata™ Screen-C GF is 70% and 270% faster than those of Xtrackt® and Certify HF®, respectively.

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Strata™ Screen-A & C

Extraction of Drugs of Abuse

Strata Screen-A and Screen-C silica-based sorbents combine hydrophobic retention of a C8 ligand plus a strong ion exchanger. The dual selectivity of these sorbents works well for extracting acidic and basic drugs of abuse from biological matrices such as serum and urine.

Strata Screen-C GF (Gravity Flow)

Strata Screen-C GF is a 200µm particle, mixed-mode sorbent that can be used to effectively clean up viscous samples and provide the following benefits:

• Superior flow in absence of a vacuum

• High extraction efficiency in comparison to traditional mixed- mode sorbents of smaller particle size

• Extraction of acidic, neutral and basic drugs

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*Contact Phenomenex for details concerning this method.

Gravity Flow Comparison Recovery of a Wide Spectrum of Analytes



Strata Screen-A and Screen-C Tubes (for drugs of abuse)


Phase 100mg 100mg 150mg 200mg 300mg 500mg 150mg 200mg 500mg

Screen-CScreen-A

8B-S016-EAK8B-S019-EAK

8B-S016-EBJ —

8B-S016-SBJ—

8B-S016-FBJ8B-S019-FBJ

8B-S016-RBJ—

——

8B-S016-SCH—

——

8B-S016-HCH8B-S019-HCH

Screen-C GF — — — 8B-S026-FBJ — 8B-S026-HBJ — 8B-S026-FCH 8B-S026-HCH


Strata™ Screen-A & C

12

Strata Screen-A and Screen-C 96-Well plates (2/Box) Phase 25mg 50mg

Screen-C 8E-S016-CGB 8E-S016-DGB

Screen-A 8E-S019-CGB 8E-S019-DGB

• Extraction of Amphetamines from Urine using Strata Screen-C (ER-001)

• Extraction of Cocaine and Benzoylecgonine from Urine using Strata Screen-C (ER-002)

• Extraction of Opiates from Urine using Strata Screen-C (ER-003)

• Extraction of PCP from Urine using Strata Screen-C (ER-004)

• Extraction of 6-MAM from Urine using Strata Screen-C (TN-005)

• Extraction of THC-COOH from Urine using Strata Screen-A (ER-006)



Pore Size: the average diameter of the porous opening on the surface of a solvent particle.For a compound to be extracted in a SPE method, the molecule must not be too large to fit into the pores. For large molecules, sorbents with extra large pores must be used.

Bed Volume: the sum of the interstitial volume plus the pore volume of the sorbent.The bed volume of the sorbent determines the solvent volumes required in a SPE method. In general, solvent volumes between 4 to 8 times the bed volume are typically necessary to ensure proper conditioning, washing and elution. Conditioning with less than 4 to 8 bed volumes increases the risk of incomplete solvation of the bed and low or irreproducible recoveries, while more than 4 bed volumes is typically unnecessary.

Conventional packed bed, silica-based SPE products typically have a bed volume of approximately 150µL per 100mg of sorbent. Polymeric sorbents require larger solvent volumes for proper conditioning, washing and eluting. It is recommended to use 200-250µL per 100mg of sorbent.

Reservoir = Sample Capacity (i.e. 1mL, 3mL, 6mL, 12mL, 20mL, 60mL)

The column volume refers to the dimensions of the SPE tube. It determines the largest volume solvent or sample that can be delivered per aliquot.

Since the majority of SPE is performed on relatively “dirty” samples that contain many potential contaminants, it is common practice to be conservative and choose a slightly larger sorbent mass.

Determining Proper Bed MassSilica-based sorbents Non-ionic sorbents retain a mass of solute (analyte plus retained contaminants) that is equivalent to approximately 5% of the sorbent mass. Therefore, a 100mg cartridge can retain approximately 5mg of total solute mass.

Polymeric sorbents The large surface area of polymers give a higher capacity that is approximately 10-15% of the sorbent mass.

Ion exchange sorbents Capacity is determined by the number of charged sites on the sorbent available to interact with the analyte.

Sorbent Bed Mass = Analyte Capacity(i.e. 30mg, 50mg, 60mg, 100mg, 150mg, 200mg, 500mg, 1g, 2g, 5g, 10g, 20g)

The sorbent bed mass determines the maximum amount of analyte that can be retained by the sorbent.

Reduce breakthrough by selecting the proper bed mass!

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n Troubleshooting




Selecting the Proper Tube

13 Tel: 310-212-0555 Fax: 310-328-7768 Web: www.phenomenex.com

Sorbent Selection

What exactly is my compound?

Separation Modes

aqueous

water

ionic

organic

organic

neutral

organic

neutral

nonpolar

reversedphase

strata-X

SDB-L

C18

C8

PH

(+)basic

ionexchange

strata-X-C

SCX

WCX

Screen-C

Screen-C GF

(-)acidic

ionexchange

SAX

NH2

Screen-A

polar

reversedphase*

strata-X

CN

neutral

moderatelypolar

reversedphase

strata-X

C8

CN

nonpolar

reversedphase

strata-X

SDB-L

C18

C8

PH

moderatelypolar

normalphase

Si-1

NH2

polar

normalphase

CN

Si-1

FL-PR

NH2

(+)basic

ionexchange

strata-X-C

SCX

WCX

Screen-C

Screen-C GF

(-)acidic

ionexchange

SAX

NH2

Screen-A

ionic

SeparationMode:

Selecting the Proper Strata Phase

*Note: These can be difficult to extract. Consider non-retentive reversed phase to remove interference.

SPE ReferenceManual & User GuideCD-Rom VersionOrder No.: AA0-6068Price:Hard Copy VersionOrder No.: AA0-6067

Price:

Polar compounds that have a log P value <1, example: Caffeine (log P= -0.07)

Moderately Polarcompounds that have a log P value between 1 and 3, example: Phenol (log P=1.46)

Non-Polarcompounds that have a log P value >3, example: Doxepin (log P = 4.29)

N

N N

N

O

O

N

O

OH

Do not know the log P values of your compound? Visit : http://esc.syrres.com/interkow/physdemo.htm

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Mechanism Typical Application

Reversed Phase Extraction of hydrophobic or polar organic analytes from aqueous matrix

Normal Phase Extraction of polar analytes from non-polar organic solvents

Ion Exchange Extraction of charged analytes from aqueous or non-polar organic samples


strata-X (patent pending)Selectivity: this revolutionary, patent-pending, polymeric SPE sorbent has numerous retention mechanisms, including hydropho-bic, hydrophilic and π-π interactions.

• General screening for acidic, neutral and basic organic compounds in same sample.

• Extracting a non-polar parent compound along with its more polar metabolite in same sample.

• Enhanced retention and clean up of polar compounds.

• Great starting point for all reversed phase applications

C18-ESelectivity: nonspecific non-polar retention mechanism with the surface end-capped to minimize secondary polar silanolinteractions.

• Extraction of hydrophobic molecules from aqueous and biological samples.

• Desalting a matrix, as ions are not retained by the sorbent.

• Separate large proteins from drugs. The drugs can be extracted from a sample, while macromolecules >15kD are too large to effectively interact with the sorbent.

C18-USelectivity: primarily non-polar retention mechanismcombined with secondary polar silanol interactions.

• Increased extraction efficiency and enhanced clean up of hydrophobic compounds that contain hydroxy or amine functional groups from water or biological fluids.

• Desalting a matrix.

• Separate large proteins from polar drugs or metabolites. The drugs can be extracted from a sample, while macromolecules >15kD are too large to effectively interact with the sorbent.

C18-T (wide pore)Selectivity: nonspecific non-polar retention mechanism withthe surface end-capped to minimize secondary polar silanolinteractions.

• Extracting large hydrophobic molecules (up to 75kD) from water or biological matrices.

• Desalting a matrix.

• Separate large proteins from drugs. The drugs can be extracted from a sample, while macromolecules >74kD are too large to effectively interact with the sorbent.

C8Selectivity: nonspecific non-polar retention mechanism with the surface end-capped to minimize secondary polar silanolinteractions.

• Extracting hydrophobic compounds from water or biological fluids that are retained too strongly on Strata C18-E or

strata-X.

PhenylSelectivity: unique non-polar retention mechanism via π-π andhydrophobic interactions.

• Extracting aromatic hydrophobic compounds.

CNSelectivity: unique selectivity toward non-polar and polarcompounds combined with secondary polar silanol interactions.

• Retaining non-polar, compounds that are retained too strongly on Strata C18-E or C8.

• A normal phase sorbent used to extract polar compounds from non-polar sorbents.

SDB-L (Styrene-divinylbenzene)Selectivity: combines non-polar retention mechanism via π-π and hydrophobic interactions completely free from secondary silanol interactions.

• Extraction of non-polar and polar molecules.

• Large volume environmental samples.

• Extracting large proteins (up to 90kD), which can effectively interact with the large pores of the sorbent, making it useful in applications such as desalting a sample.

Reversed Phases

Reversed Phase Material CharacteristicsPhase Particle Size

(µm)Pore Size

(Å)Surface Area

(m2/g)Carbon Load

(%)Bonding End Capping

strata-X 33 85 800 – – No

C18-E 55 70 500 17.0 trifunctional Yes

C18-U 55 70 500 18.0 trifunctional No

C18-T 55 140 300 15.0 trifunctional Yes

C8 55 70 500 10.5 trifunctional Yes

Phenyl 55 70 500 10.5 trifunctional Yes

CN 55 70 500 10.0 trifunctional No

SDB-L 100 260 500 – – No


Normal Phases

Silica (Si-1)*Selectivity: unbonded surface results in hydrogen bonding, which gives strong retention mechanism for polar compounds.

• Extraction of polar compounds that are similar in structure.

CNSelectivity: dual selectivity toward polar and non-polar compounds combined with secondary polar silanol interactions.

• Normal phase sorbent that can effectively extract polar compounds from non-polar solvents.

• Extracting polar compounds that may be retained too strongly on Strata Si-1 such as hydroxyl or amino groups.

Florisil (Pesticide Residue)Selectivity: polar retention attributed to magnesium ion on the silica surface.

• Extraction of pesticides from environmental samples.

• Extraction of halogented compounds.

NH2Selectivity: retention mechanism of polar analytes by either hydrogen bonding or weak anion exchange.

• Extraction of strong anions from aqueous samples.

• Extraction of polar compounds similar in structure.

• Extracting polar compounds that may be retained too strongly on Strata Si-1 such as hydroxyl groups.

Normal Phase Material CharacteristicsPhase Particle Size

(µm)Pore Size

(Å)Surface Area

(m2/g)Carbon Load

(%)Bonding End Capping

Si-1 (Silica) 55 70 550 – – –

FL-PR (Florisil) 170 80 300 – – –

CN 55 70 500 10.0 trifunctional No

NH2 55 70 500 5.0 trifunctional No

*Silica is hydroscopic, any water adsorbed to the surface can greatly reduce the retention of organic solutes. It is important to keep silica sorbents dry and free from water.

16Tel: 310-212-0555 Fax: 310-328-7768 Web: www.phenomenex.com

17

Ion Exchange Phases

strata-X-C (patent pending)Selectivity: unique selectivity of strata-X plus strong cationexchanger to give mixed-mode retention of target analytes.

• Extraction of aromatic bases by π-π selectivity.

• Aggressive clean up of basic compounds from organic solvents.

• Fractionation of acidic and neutral components from basic analytes.

WCX (weak cation exchange)Selectivity: unique selectivity of carboxylic acid (CBA)bonded to silica surface.

• Extraction of quaternary amines.

• Extraction of strong cations.

SCX (strong cation exchange)Selectivity: surface bonded benzene sulfonic acid groupfor the retention of organic bases from biological andaqueous sample matrices.

• Extraction of 1°, 2° and 3° amines from biological fluids.

• π-π selectivity of benzene ring allows for some hydrophobic retention of analytes.

Screen-CSelectivity: incorporates the selectivity of a C8 plus astrong cation exchanger to give mixed-mode retention fortarget analytes.

• Extraction of basic drugs from biological matrices such as blood, serum and urine.

• Available in gravity flow format, 200 µm particle size (Screen-C GF) for viscous samples such as horse urine and plasma.

SAX (strong anion exchange)Selectivity: unique selectivity of a quaternary amine always charged which allows for retention of analytes from biological and aqueous matrices.

• Extraction of organic acids.

• Extraction of biomolecules.

WAX (weak anion exchange)Selectivity: NH2 bonded to silica surface to give retentionmechanism by either hydrogen bonding or weak anionexchange.

• Extraction of strong ions from aqueous solvent.

• Extraction of biomolecules.

Screen-ASelectivity: incorporates the selectivity of a C8 plus a strong anion exchanger to give mixed-mode retention for target analytes.

• Extraction of acidic drugs from biological matrices such as blood, serum and urine.

Ion Exchange Phase Material CharacteristicsPhase Particle Size

(µm)Pore Size

(Å)Surface Area

(m2/g)Carbon Load

(%)Bonding End

CappingpKa Ionic Capacity

(meq/g)

strata-X-C 33 85 800 – – – <1.0 1.0

WCX 55 70 500 8.0 trifunctional No 4.5 0.8

SCX 55 70 500 9.0 trifunctional No <1.0 0.9

Screen-C 55 70 500 proprietary trifunctional – <1.0 proprietary

Screen-C GF 200 70 500 proprietary trifunctional – <1.0 proprietary

SAX 55 70 500 6.5 trifunctional No Alwayscharged

0.9

NH2 (WAX) 55 70 500 5.0 trifunctional No 9.8 1.3

Screen-A 55 70 500 proprietary trifunctional – Alwayscharged

proprietary

Ansys Captiva® and Bond Elut Certify® are registered trademarks of Varian Inc. Xtrackt® is a registered trademark of United Chemical Technologies Inc. Empore™ is a trademark of 3M. Strata™, Sepra™ and Zebron™ are a trademark of Phenomenex Inc. Luna® is a registered trademark of Phenomenex Inc. Phenomenex is in no way affiliated with Varian, United Chemical Technologies Inc., or 3M. Applications shown may not be representative of all SPE applications.


18

Strata™ SPE TubesIf Strata SPE or Sepra Bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE products or Sepra Bulk for FREE!

SPE ReferenceManual & User GuideCD-Rom VersionOrder No.: AA0-6068Price: Hard Copy VersionOrder No.: AA0-6067

Price:


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Strata Tubes


Phase 50mg 100mg 100mg 200mg 500mg 500mg 1g

SDB-L — 8B-S014-EAK — 8B-S014-FBJ 8B-S014-HBJ 8B-S014-HCH 8B-S014-JCH

C18-E 8B-S001-DAK 8B-S001-EAK 8B-S001-EBJ 8B-S001-FBJ 8B-S001-HBJ 8B-S001-HCH 8B-S001-JCH

C18-U — 8B-S002-EAK — 8B-S002-FBJ 8B-S002-HBJ 8B-S002-HCH 8B-S002-JCH

C18-T — 8B-S004-EAK — 8B-S004-FBJ 8B-S004-HBJ 8B-S004-HCH 8B-S004-JCH

C8 — 8B-S005-EAK — 8B-S005-FBJ 8B-S005-HBJ 8B-S005-HCH 8B-S005-JCH

Phenyl — 8B-S006-EAK — 8B-S006-FBJ 8B-S006-HBJ — 8B-S006-JCH

SCX — 8B-S010-EAK 8B-S010-EBJ 8B-S010-FBJ 8B-S010-HBJ 8B-S010-HCH 8B-S010-JCH

WCX — 8B-S027-EAK — 8B-S027-FBJ 8B-S027-HBJ 8B-S027-HCH 8B-S027-JCH

SAX — 8B-S008-EAK 8B-S008-EBJ 8B-S008-FBJ 8B-S008-HBJ 8B-S008-HCH 8B-S008-JCH

NH2 — 8B-S009-EAK — 8B-S009-FBJ 8B-S009-HBJ 8B-S009-HCH 8B-S009-JCH

CN — 8B-S007-EAK — 8B-S007-FBJ 8B-S007-HBJ 8B-S007-HCH 8B-S007-JCH

Si-1 — 8B-S012-EAK — 8B-S012-FBJ 8B-S012-HBJ 8B-S012-HCH 8B-S012-JCH

Florisil — — — — 8B-S013-HBJ 8B-S013-HCH 8B-S013 -JCH


19

Strata™ Giga™ Tubes

Benefits of Strata Giga Tubes • Large analyte capacity• Available in 12, 20, 60, and 150mL tube sizes for large volume samples• Pre-packed sorbent bed for consistent recoveries• Great alternative to Varian Mega Bond Elut Flash® and ISOLUTE® Flash columns • Can be used to directly scale-up from smaller SPE formats

Note: tube sizes not proportional nor actual size.*Available in 50g and 70g/150mL formats

Strata Giga Tube Ordering Information


Phase 100mg 500mg 1g 2g 1g 5g 10g 20g

C18-E — 8B-S001-HDG — 8B-S001-KDG — 8B-S001-LEG 8B-S001-MFF 8B-S001-VFF

C8 — — — 8B-S005-KDG — 8B-S005-LEG 8B-S005-MFF —

Phenyl — — — 8B-S006-KDG — 8B-S006-LEG 8B-S006-MFF —

SCX — 8B-S010-HDG — 8B-S010-KDG — 8B-S010-LEG 8B-S010-MFF 8B-S010-VFF

SAX — 8B-S008-HDG — 8B-S008-KDG — 8B-S008-LEG 8B-S008-MFF 8B-S008-VFF

NH2 — 8B-S009-HDG — 8B-S009-KDG — 8B-S009-LEG 8B-S009-MFF 8B-S009-VFF

CN — — — 8B-S007-KDG — 8B-S007-LEG 8B-S007-MFF —

Si-1* — 8B-S012-HDG — 8B-S012-KDG — 8B-S012-LEG 8B-S012-MFF 8B-S012-VFF

Florisil — — — 8B-S013-KDG — 8B-S013-LEG 8B-S013-MFF —

strata-X — 8B-S100-HDG 8B-S100-JDG — 8B-S100-JEG — — —

strata-X-C 8B-S029-EDG 8B-S029-HDG 8B-S029-JDG — — — — —

A. Silica-based

Giga Format 500mg/12mL 2g/12mL 5g/20mL 10g/60mL 20g/60mL

Retention Capacity† 25mg 100mg 250mg 500mg 1000mg

Suggested Elution Volume† 5mL 20mL 50mL 100mL 200mL

B. Polymeric-based

Giga Format 100mg/12mL 500mg/12mL 1g/12mL 1g/20mL

Retention Capacity† 15mg 75mg 150mg 150mg

Suggested Elution Volume† 2mL 10mL 20mL 20mL

†Retention capacities and elution volumes are specific to the chemical nature of the analyte being extracted, its concentration in the sample, the chemical nature of the eluting solvent and the bed mass used. The above are only guidelines. An elution study should be conducted. As a general rule of thumb recommended solvent wash and elution volumes for silica-based material should be 150µL per 100mg of sorbent and 200µL per 100mg of polymeric sorbent. ISOLUTE® Flash columns are registered trademarks of Argonaut Technologies Inc. Varian Mega Bond Elut® is a registered trademark of Varian Inc.

New Format


Strata Method Development 96-well plate • Each Strata 96-well plate contains six silica-based sorbents and

two polymeric resins. One row of C18-E, C18-U, C18-M, C18-T, C8, phenyl (50mg/well) and strata-X and SDB-L (30mg/well) for parallel screening.

Order No. Unit/box Price

KS0-7293 1

96-well plate AccessoriesOrder No. Description Unit Price

Manifold

AH0-7284 96-Well Plate Manifold, Acrylic 1 ea

Sealing Mats

AH0-7195 Pierceable sealing mats, 96 square well 50/pk

AH0-7362 Sealing tape pad 10/pk

Collection Plates

AH0-7192 0.35mL/well 96 square well collection plate 50/pk

AH0-7193 1mL/well 96 square well collection plate 50/pk

AH0-7194 2mL/well 96 square well collection plate 50/pk

AH0-7408 Solvent waste reservoir tray 25/pk


Strata 96-well plate 2 Plates/Box

Manifold Replacement Gaskets available, contact your Phenomenex Technical Consultant.

96-Well Plates

20

Phase 5mg 10mg 15mg 25mg 30mg 50mg 60mg 100mg

strata-X-C — 8E-S029-AGB — — 8E-S029-TGB — 8E-S029-UGB —

strata-XSDB-L

8E-S100-NGB—

8E-S100-AGB—

——

—8E-S014-CGB

8E-S100-TGB—

—8E-S014-DGB

8E-S029-UGB—

——

— —

Screen-CScreen-A

——

——

——

8E-S016-CGB8E-S019-CGB

——

8E-S016-DGB8E-S019-DGB

——

——

C18-EC18-UC8PhenylNH2

CNWCXSCXSAXSi-1

——————————

——————————

8E-S001-BGB—————————

8E-S001-CGB—

8E-S005-CGB8E-S006-CGB8E-S009-CGB8E-S007-CGB8E-S027-CGB8E-S010-CGB8E-S008-CGB8E-S012-CGB

——————————

8E-S001-DGB8E-S002-DGB8E-S005-DGB8E-S006-DGB8E-S009-DGB8E-S007-DGB8E-S027-DGB8E-S010-DGB8E-S008-DGB8E-S012-DGB

——————————

8E-S001-EGB—

8E-S005-EGB8E-S006-EGB8E-S009-EGB8E-S007-EGB8E-S027-EGB8E-S010-EGB8E-S008-EGB8E-S012-EGB

Compatible with most liquid handling systems and robotic workstations


Sepra™

Sepra is a high performance bulk SPE sorbents. Works well for applications such as MSPD*(matrix solid phase dispersion) in which drugs of interest are separated from homogenized animal tissue or food sources, and can also be used for flash analysis to effectively purify organic compounds. Overall Sepra sorbents are:

• Economical

• Rugged

• Available in polar and non-polar phases for wide range of selectivity

• Available in gram to multi-kilogram quantities

If Sepra Bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Sepra Bulk for FREE!

*Interested in MPSD for your analysis? Please contact your Phenomenex Technical Consultant for technique and accessory information.

Sepra Material CharacteristicsPhase Particle

Size (µm)

Pore Size(Å)

Surface Area

(m2/g)

Carbon Load

%

Bonding End Capping

pH Stability

Ionic Capacity (meq/g)

Reversed Phase

C18-E C18-T C8Phenyl CN SDB-L

505050505095

65140656565225

520300520520520520

trifunctionaltrifunctionaltrifunctionaltrifunctionaltrifunctional

—

YesYesYesYesNoNo

1 to 81 to 81 to 81 to 81 to 8

1 to 14

——————

Normal Phase

Silica FL-PR (Florisil)

50165

6575

300300

——

NoNo

1 to 81 to 8

——

Ion Exchange

SCX SAXNH2

505050

656565

520520520

trifunctionaltrifunctionaltrifunctional

NoNoNo

1 to 81 to 81 to 8

0.90.91.3

Sepra Ordering InformationPhase 100g Price 1kg Price

Reversed Phase

C18-E C18-T (wide pore)C8Phenyl CN SDB-L

04G-434804G-440504G-440604G-440704G-440904G-4412

04K-434804K-440504K-440604K-440704K-440904K-4412

Normal Phase

Silica FL-PR (Florisil)

04G-441004G-4411

04K-441004K-4411

Ion Exchange

SCX SAXNH2

04G-441304G-441404G-4408

04K-441304K-441404K-4408

17.015.010.510.59.8—

——

9.06.55.0


Vacuum Manifolds

Vacuum Manifold Replacement Parts

Adaptor Caps

Waste Container

Polypropylene Needles

Collection Racks

Stopcocks

24 – Position Vacuum Manifold

AH0-6024

SPE 24-Position Vacuum Manifold, Complete Assembly for 1 eafor 1, 3, 6mL Tubes


AH0-6023

SPE 12-Position Vacuum Manifold,Complete Assembly 1 ea for 1, 3, 6mL Tubes


AH0-7502

SPE 10-Position Tall-Boy Vacuum Manifold Complete Assembly 1 ea for Giga Tubes

SPE Accessories

Ordering InformationOrder No. Description Unit Price

24 – Position Vacuum Manifold Replacement Parts

AH0-6026AH0-6028AH0-6030AH0-6032AH0-6038

SPE Glass ChamberSPE Cover, Gasket and 24 StopcocksSPE GasketsSPE Vacuum GaugeSPE Collection Rack, including shelves, legs, clips and posts

eaea

2/pkeaea

AH0-6041AH0-6044

SPE Plate for 13mm Test TubeSPE Plate for 16mm Test Tube

eaea

AH0-6049AH0-6051

SPE StopcocksSPE Drying Attachment

24/pkea


AH0-6025AH0-6027AH0-6029AH0-6031AH0-6037

SPE 12-Position Glass ChamberSPE Cover, Gasket and 12 StopcocksSPE GasketsSPE Vacuum Gauge, Valve and Glass ChamberSPE Collection Rack, including shelves, legs, clips and posts

eaea

2/pkeaea

AH0-6040AH0-6043AH0-6042AH0-6045AH0-6046AH0-6047

SPE Plate for 13mm Test TubeSPE Plate for 16mm Test TubeSPE Plate for Volumetric FlaskSPE Plate for Autosampler VialSPE Plate, DimpleSPE Plate, Base

eaeaeaeaeaea

AH0-6048AH0-6050AH0-6052

SPE StopcocksSPE Drying AttachmentSPE 12-Position Vacuum Waste Container, polyproplyene

12/pkea

10/pk


AH0-7503 SPE 10-Position Tall-Boy Vacuum Manifold Glass Chamber ea

AH0-7504 SPE 10-Position Tall-Boy Vacuum Manifold Cover, Gasket and 10 Stopcocks

ea

General Vacuum Manifold Accessories

AH0-6033AH0-6034AH0-6035AH0-6036

SPE Manifold Needles, polypropyleneSPE Manifold Needles, polypropyleneSPE Manifold Needles, stainless steelSPE Manifold Needles, stainless steel

12/pk24/pk12/pk24/pk

AH0-6053AH0-6054

Female Luer FittingsMale Luer Fittings

2/pk2/pk

AH0-6055AH0-6056

Support Posts for RackLegs for Cover, black

3/pk4/pk

AH0-6057AH0-6058AH0-6059

Vacuum Gauge and Valve AssemblyValve Assembly OnlyVacuum Gauge Only

eaeaea

AH0-6060AH0-6061

Retaining ClipsVacuum Manifold Plugs

12/pk50/pk

AH0-6062AH0-6063

Control Valve, Teflon®

Control Valve, Teflon®

25/pk50/pk

AH0-6064AH0-6065

Teflon® NeedlesTeflon® Needles

100/pk500/pk

AH0-7191AH0-7378AH0-7379

Adaptor Caps for 1, 3 and 6mL SPE tubesAdaptor Caps for 12 and 20mL SPE tubesAdaptor Caps for 60mL SPE tubes

15/pk5/pk5/pk

22

Use an adaptor cap to attach an empty SPE tube.

Adaptor Cap(AH0-7191)


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Documents

2242 Strata Br - Chromservis Strata Full... · The Strata line is comprised of ultra-pure sorbents, which are designed to give high recoveries for traditional SPE applications such