S-1

Supporting Information

Authors names:

Stephanie Moore

Stephanie Hess

James Jorgenson

Affiliation:

The University of North Carolina at Chapel Hill

Article Title:

Extraction, Enrichment, Solubilization, and Digestion Techniques for Membrane Proteomics

Table of Contents:

Table S-1. Improved Protocol vs. FASP Protocol with yeast cell lysate-----...-...page S-2

Table S-2. Default digestion protocol----------------------.page S-3

Table S-3. Protein Lynx Global Server (PLGS) V2.5 settings-----------...page S-4

Table S-4. Proteins identified in comparison of the Improved vs. Default protocol---page S-5

S-2

Table S-1. Shotgun digestion protocols: Improved Protocol vs. FASP Protocol with yeast cell lysate.

Improved Protocol 1. 50 µL of lysed yeast cell lysate (2.24

mg/mL), 40 µL ammonium bicarbonate (50 mM, pH 7.8) and 20 µL of 5 % sodium deoxycholate solution.

2. Sonicated solution for 10 min and placed at 80 °C for 10 min.

3. Add 10 mM dithioerythritol, sonicate 10

min, place at 80 °C for 10 min.

4. Add 20 mM iodoacetamide, sonicate 10 min, incubate in dark 30 min at room temperature.

5. Add trypsin 1:30 trypsin:protein concentration. Digest overnight 37 °C.

6. Acidify to give 0.5-1 % TFA.

7. Sit for 2 hr at 60 °C, centrifuge 15,000 rpm for 5 min and collect supernatant.

Shotgun Protein Identification Results: Sample 1 = 193 Sample 2 = 211 Sample 3 = 202

FASP Protocol [37, 55] 1. 50 µL of lysed yeast cell lysate (2.24

mg/mL) and 150 µL of lysis solution containing 4 % SDS in 100 mM Tris/HCl pH 7.6 and 0.1 M dithioerythritol placed in centrifuge tube.

2. Placed at 95 °C for 3 min, centrifuged

15,000 rpm for 15 min. 3. 30 k filters (Microcon YM-30) were

washed twice with 200 µL of UA solution (8M urea in 0.1 Tris/HCl pH 8.5) by centrifugation 15,000 rpm for 15 min.

4. Reduced yeast protein solution was

added to filter with 200 µL of UA solution, centrifuged 14,000 rpm for 15 min. Wash again with 200 µL of UA solution.

5. 100 µL of 0.05 M iodoacetamide solution

in UA was added and allowed to incubate for 20 min at room temperature.

6. Two washings of 100 µL of UA solution,

centrifuged 14,000 rpm for 15 min. 7. Two washings of 100 µL of 50 mM

ammonium bicarbonate solution, centrifuged 14,000 rpm for 10 min.

8. 40 µL of trypsin stock (0.4 µg/µL) added

to solution with 80 µL of 50 mM ammonium bicarbonate to wet/cover filter. Sample was mixed and incubated overnight at 37 °C room temperature.

9. Sample was centrifuged at 14,000 rpm

for 10 min to collect peptides, washed with 40 µL of 50 mM ammonium bicarbonate and acidified to give 0.5-1 % TFA.

Shotgun Protein Identification Results: Sample 1 = 5 Sample 2 = 1 Sample 3 = 3

S-3

Table S-2. Default digestion protocol adapted from: (http://www.genome.duke.edu/cores/proteomics/sample-preparation)45

Procedure:

a) Perform Bradford assay to determine protein concentration of each solution. Normalize concentrations for samples within a group using 50 mM AmBic.

b) In order to maximize solubility of proteins, add calculated volume of Waters RapiGest

to have 0.1-0.2 % RapiGest in final concentration pre-digestion. Make up RapiGest with 50 mM AmBic.

c) Heat at 40°C for 10 minutes. Spin down condensate. d) Make up 100 mM DTT in 50 mM AmBic for reduction step. Add DTT to each solution

to make the final DTT concentration 10 mM. e) Heat solution at 80°C for 15 minutes. f) Remove from heat and cool for 5 minutes (to room temp). Spin down condensate. g) Make up 200 mM iodoacetamide (IAM) in 50 mM AmBic for alkylation step. Add IAM

to each solution to make the final IAM concentration 20 mM (2X molar excess of DTT).

h) Incubate the solutions in the dark at room temperature for 30 minutes. i) Add trypsin to each solution at 1:30 trypsin:protein concentration. Make trypsin stock

in 50 mM AmBic. j) Digest overnight at 37°C. k) Following digestion, centrifuge condensate to bottom of vial. Add TFA and MeCN to

give 0.5-1.0% TFA and 2% MeCN by volume.

l) Samples sit for 2 hours at 60°C. Centrifuge at 15,000 rpm for 5 min, and pipette supernatant into an autosampler vial.

S-4

Table S-3. Protein Lynx Global Server (PLGS) V2.5

Processing Parameters in PLGS V2.5

Chromatography peak width automatic

MS TOF resolution automatic 10,000

Lock Mass 785.84265 Da/e Glu-1-Fibrinopeptide

Lock Mass Window 1 Da

Low Energy Threshold 150 counts

Elevated Energy Threshold 50 counts

Intensity Threshold 750 counts

Workflow Template in PLGS V2.5

Databank S.cerevisiae from http://www.expasy.org/proteomics

Peptide tolerance automatic*

Fragment tolerance automatic*

Minimum fragment ion matches per peptide 3

Minimum fragment ion matches per protein 7

Minimum peptide matches per protein 1

Maximum hits 20

Maximum Protein Mass 250,000

Primary Digest Reagent (selection varied depending on study: trypsin, chymotrypsin, or pepsin)

Secondary Digest Reagent (used for tandem digestions)

Missed Cleavages 1

Fixed Modifications Carbamidomethyl C

Variable Modifications Oxidation M, Deamidation N, Deamidation Q

False Positive Rate 4

* When “automatic” is selected the proprietary PLGS algorithm interrogates each data set to determine an ideal value for the analysis.

S-5

Table S-4. Proteins identified in comparison of the Improved vs. Default protocol. All reported

proteins were required to be identified in at least 2 of the 3 replicate UHPLC-MS run.

*The PLGS Score is generated taking into account several factors, including confidence of the

identification.

Fo

un

d In

Pro

tein

Typ

e

mW

(Da)

pI (p

H)

PL

GS

Sco

re*

To

tal #

Pep

tides

Co

vera

ge %

Pro

du

ct Io

ns

Dig

est

Pep

tides

Mo

difie

d

Pep

tides

Default A0A0A7NYF6_YEAST membrane 58759 6.6 563 5 6 52 4 1

Default ATP10_YEAST membrane 32073 9.9 1633 6 29 38 6 0

Default ATP19_YEAST membrane 7529 9.6 8224 5 51 39 4 1

Default ATP23_YEAST membrane 26873 7.5 953 5 26 21 4 0

Default ATP5E_YEAST membrane 6738 10.1 1598 3 68 22 3 0

Default COX8_YEAST membrane 8900 10.8 5325 5 32 42 4 0

Default ERG26_YEAST membrane 38681 6.2 1238 17 47 71 13 1

Default ISD11_YEAST membrane 11258 10.7 1789 4 47 25 4 0

Default MIC60_YEAST membrane 61044 6.2 1243 33 51 210 24 8

Default PSA4_YEAST membrane 28421 7.2 2135 18 58 87 11 2

Default PSA5_YEAST membrane 28599 4.5 1985 15 55 65 12 2

Default PSA6_YEAST membrane 25588 7.2 3518 11 47 68 8 0

Default SSO2_YEAST membrane 33712 4.6 2080 15 34 115 14 6

Default VPS4_YEAST membrane 48142 4.9 1259 21 45 97 14 2

Default YBR4_YEAST membrane 16028 6.8 614 2 28 9 2 0

Default YMN1_YEAST membrane 39951 8.3 1649 9 28 58 8 2

Default YP036_YEAST membrane 7050 5.4 2978 5 84 38 5 2

Default YPT53_YEAST membrane 24632 4.9 1063 16 46 83 12 2

Default AIM9_YEAST non-membrane 72368 8.9 1467 31 49 152 24 3

Default APT1_YEAST non-membrane 20573 4.8 1593 5 36 38 5 0

Default CDK1_YEAST non-membrane 34039 8.5 2328 18 45 75 12 1

Default CPYI_YEAST non-membrane 24341 6.1 902 14 46 70 9 1

Default CYS3_YEAST non-membrane 42516 6.1 1142 16 53 78 13 1

Default DBP1_YEAST non-membrane 67875 9.1 1022 24 35 132 19 3

Default DUG1_YEAST non-membrane 52837 5.3 1914 20 46 135 15 2

Default FSH1_YEAST non-membrane 27322 5.9 1225 10 51 65 10 1

Default GCSH_YEAST non-membrane 18781 4.5 1456 10 42 72 6 2

Default GUAA_YEAST non-membrane 58445 6.0 1198 32 57 167 23 5

Default H2A2_YEAST non-membrane 13980 11.1 3914 15 67 99 8 1

Default HIBCH_YEAST non-membrane 56252 8.4 761 18 37 78 13 4

S-6

Default HIS1_YEAST non-membrane 32246 5.8 955 16 47 98 11 2

Default HOSC_YEAST non-membrane 47069 6.9 1109 19 51 105 17 2

Default HSP42_YEAST non-membrane 42790 4.8 946 11 30 64 8 1

Default MLC1_YEAST non-membrane 16434 4.4 2224 10 54 48 7 0

Default MPD2_YEAST non-membrane 32380 6.1 1827 7 21 55 5 2

Default NAP1_YEAST non-membrane 47855 4.0 1142 13 52 68 12 3

Default ODPX_YEAST non-membrane 45333 5.4 2501 16 45 117 15 4

Default OYE3_YEAST non-membrane 44892 5.3 767 11 34 86 11 5

Default PRS8_YEAST non-membrane 45243 9.4 1082 19 48 115 18 4

Default RGI2_YEAST non-membrane 19216 7.0 1791 6 37 36 5 0

Default RL13B_YEAST non-membrane 22511 11.5 1019 6 29 36 4 0

Default RL20A_YEAST non-membrane 20423 10.7 1394 10 49 72 10 0

Default RL2B_YEAST non-membrane 27391 11.5 1512 16 45 95 13 4

Default RM07_YEAST non-membrane 33078 10.1 4136 11 46 96 10 2

Default RPAB1_YEAST non-membrane 25062 9.4 3178 11 52 74 10 2

Default RS17A_YEAST non-membrane 15778 10.9 7224 12 51 58 7 0

Default RS29A_YEAST non-membrane 6656 10.7 5629 10 59 38 6 1

Default RT01_YEAST non-membrane 36705 9.4 650 9 32 52 8 2

Default SDHF2_YEAST non-membrane 19029 9.2 2818 9 39 46 7 0

Default SDO1L_YEAST non-membrane 12002 4.8 6668 10 68 74 8 0

Default SEC18_YEAST non-membrane 84003 8.1 913 34 43 162 28 7

Default SERA_YEAST non-membrane 51161 5.2 1353 24 45 107 20 5

Default SGT2_YEAST non-membrane 37195 4.5 2135 23 77 104 19 4

Default SUI1_YEAST non-membrane 12304 8.2 2712 7 49 35 3 0

Default YNE0_YEAST non-membrane 50932 6.1 1125 11 25 63 9 1

Default YI115_YEAST unknown 13907 7.1 987 2 36 17 2 0

Default YI156_YEAST unknown 8159 9.5 4330 4 56 38 3 0

Improved AAKG_YEAST membrane 36378 5.4 914 15 56 104 14 5

Improved ADT1_YEAST membrane 34098 10.0 15697 42 36 361 25 18

Improved AIM19_YEAST membrane 16912 10.3 4457 15 54 98 9 2

Improved ATC6_YEAST membrane 135182 6.1 807 21 23 148 18 6

Improved ATG27_YEAST membrane 30182 5.4 2245 12 22 82 9 2

Improved ATP14_YEAST membrane 14119 4.4 2223 2 11 19 1 0

Improved CBP3_YEAST membrane 39059 9.6 932 14 40 74 13 2

Improved CDC10_YEAST membrane 37002 5.4 2623 23 61 112 17 5

Improved COPG_YEAST membrane 104765 4.8 2716 25 31 222 20 3

Improved COX14_YEAST membrane 7953 7.5 6909 25 59 135 9 4

Improved COX2_YEAST membrane 28548 4.2 5312 14 31 142 11 3

S-7

Improved COX7_YEAST membrane 6927 10.9 5891 10 30 50 3 1

Improved CP51_YEAST membrane 60681 8.9 1623 14 30 94 10 1

Improved DLD1_YEAST membrane 65251 6.4 1877 6 15 40 6 0

Improved ELO3_YEAST membrane 39439 9.5 991 3 14 46 3 0

Improved ENT3_YEAST membrane 45063 4.5 1372 17 33 84 13 2

Improved ERD2_YEAST membrane 25745 10.0 957 7 16 27 4 0

Improved ERG2_YEAST membrane 24879 5.5 6875 9 27 81 7 2

Improved ERV14_YEAST membrane 15919 6.5 5179 1 8 17 1 0

Improved ERV29_YEAST membrane 34990 9.9 912 4 11 37 3 0

Improved ERV41_YEAST membrane 40679 4.6 982 5 23 41 5 0

Improved GAS1_YEAST membrane 59544 4.3 2139 19 28 164 15 3

Improved GET1_YEAST membrane 27075 9.6 1641 5 22 32 5 0

Improved GET2_YEAST membrane 31473 9.8 2319 3 8 38 3 1

Improved GNT1_YEAST membrane 57541 8.5 867 18 46 89 16 5

Improved GPI16_YEAST membrane 68727 5.0 1032 17 32 83 16 4

Improved GPX2_YEAST membrane 18394 8.8 3483 17 65 90 11 3

Improved HACD_YEAST membrane 24494 10.2 7980 9 32 75 6 2

Improved HXT7_YEAST membrane 62694 7.7 2166 24 33 189 18 5

Improved KRE2_YEAST membrane 51354 5.1 1153 18 34 85 11 1

Improved MBA1_YEAST membrane 31791 10.2 1870 19 60 112 13 4

Improved MDM38_YEAST membrane 64964 7.9 2461 34 40 174 23 7

Improved MIC27_YEAST membrane 26943 9.7 2779 17 55 93 14 3

Improved MKAR_YEAST membrane 38683 9.8 2036 8 33 59 7 0

Improved MPC1_YEAST membrane 14985 9.8 1950 9 45 40 6 0

Improved MPC3_YEAST membrane 16219 10.9 9584 9 56 76 8 1

Improved PAM17_YEAST membrane 21954 10.0 2394 6 41 42 4 2

Improved PEM2_YEAST membrane 23134 9.2 956 6 47 45 6 2

Improved PET10_YEAST membrane 31227 8.7 1601 6 29 56 6 2

Improved PEX11_YEAST membrane 26858 10.2 2687 10 43 63 9 2

Improved PHO86_YEAST membrane 34859 10.0 6006 17 42 118 12 1

Improved PLB1_YEAST membrane 71622 4.3 1189 15 23 86 15 1

Improved PMT1_YEAST membrane 92615 6.2 690 17 23 115 15 3

Improved PSA3_YEAST membrane 28696 4.9 2909 14 37 93 9 0

Improved PSA7_YEAST membrane 31516 4.8 1197 17 59 84 14 3

Improved PSS_YEAST membrane 30784 5.8 3416 9 24 74 9 3

Improved QCR6_YEAST membrane 17246 3.8 2437 4 37 19 3 1

Improved RER1_YEAST membrane 22255 8.6 2269 7 27 50 5 1

Improved RV161_YEAST membrane 30231 6.2 1471 16 33 79 12 3

Improved SCO1_YEAST membrane 33145 9.4 926 14 39 64 10 1

S-8

Improved SCO2_YEAST membrane 34866 8.1 1309 13 59 70 9 1

Improved SCS7_YEAST membrane 44852 6.1 3091 7 18 57 6 0

Improved SEC11_YEAST membrane 18749 9.5 2840 13 66 96 10 3

Improved SEC24_YEAST membrane 103569 5.8 2461 42 36 321 32 5

Improved SOP4_YEAST membrane 26580 9.7 2518 11 28 60 7 1

Improved STT3_YEAST membrane 81476 8.0 839 11 24 67 11 1

Improved TAM41_YEAST membrane 44171 9.9 1379 25 50 106 19 3

Improved TCB1_YEAST membrane 133492 6.8 2248 40 31 278 34 10

Improved TECR_YEAST membrane 36744 9.8 1810 11 24 75 9 0

Improved TIM8_YEAST membrane 9757 5.1 1005 5 33 21 3 0

Improved TOM7_YEAST membrane 6865 9.5 19375 1 22 14 1 0

Improved VTC1_YEAST membrane 14361 10.9 2578 10 37 58 7 0

Improved YET1_YEAST membrane 23444 10.1 8532 6 33 63 6 1

Improved YET3_YEAST membrane 22888 7.0 4268 5 19 40 4 0

Improved YIP5_YEAST membrane 34821 4.6 1328 2 12 22 2 0

Improved YJR1_YEAST membrane 42930 4.7 3274 17 43 139 13 2

Improved YL099_YEAST membrane 9979 4.1 5403 2 26 19 2 0

Improved YTA12_YEAST membrane 93218 7.4 1307 30 31 165 25 1

Improved 2AAA_YEAST non-membrane 70863 4.4 640 11 30 66 11 2

Improved 6PGD2_YEAST non-membrane 53888 6.9 2999 43 42 265 29 12

Improved AAP1_YEAST non-membrane 97601 5.0 1045 39 45 228 30 6

Improved ACOX_YEAST non-membrane 83989 8.6 4153 45 46 236 32 3

Improved ADH5_YEAST non-membrane 37624 5.9 925 15 40 75 10 2

Improved AIM41_YEAST non-membrane 21215 10.0 2967 21 65 95 15 1

Improved AIM46_YEAST non-membrane 34091 9.4 5123 5 18 39 5 1

Improved APE2_YEAST non-membrane 107686 8.1 1404 49 40 263 36 4

Improved ARO1_YEAST non-membrane 174643 5.8 1175 59 43 293 45 7

Improved ARO9_YEAST non-membrane 58490 5.2 852 21 30 104 16 5

Improved ARPC5_YEAST non-membrane 17123 4.9 1413 9 55 34 6 2

Improved ATP11_YEAST non-membrane 36827 9.4 810 20 39 69 12 3

Improved CAP_YEAST non-membrane 57486 5.3 914 16 28 65 13 3

Improved CSK22_YEAST non-membrane 39378 8.7 2936 19 34 93 13 1

Improved DHAS_YEAST non-membrane 39518 6.3 1354 18 31 87 10 3

Improved DHE5_YEAST non-membrane 49595 5.2 3111 32 57 163 21 3

Improved DHH1_YEAST non-membrane 57507 7.9 1917 18 40 114 15 5

Improved EIF3G_YEAST non-membrane 30482 6.4 3534 13 47 58 10 1

Improved EMI2_YEAST non-membrane 55885 5.8 2184 12 26 57 8 1

Improved ERF3_YEAST non-membrane 76503 6.6 1104 19 26 120 15 2

Improved EUG1_YEAST non-membrane 58945 4.6 1547 15 31 96 14 2

S-9

Improved FRDS_YEAST non-membrane 50812 5.9 1213 14 33 95 13 1

Improved FUMH_YEAST non-membrane 53118 8.3 1745 28 31 116 13 2

Improved GAL80_YEAST non-membrane 48292 8.1 1046 14 37 88 13 5

Improved GET3_YEAST non-membrane 39328 4.7 1180 13 22 75 8 1

Improved GET4_YEAST non-membrane 36257 4.9 2990 18 47 135 13 3

Improved GLYM_YEAST non-membrane 53652 9.0 2323 33 43 126 18 4

Improved GPD1_YEAST non-membrane 42841 5.2 1888 11 40 82 10 1

Improved GPD2_YEAST non-membrane 49390 6.6 757 9 30 68 9 2

Improved GYS2_YEAST non-membrane 80028 5.9 804 16 20 73 11 1

Improved H2AZ_YEAST non-membrane 14274 11.1 4977 10 49 47 5 1

Improved HMF1_YEAST non-membrane 13897 5.1 3173 9 65 53 6 1

Improved HSP31_YEAST non-membrane 25654 5.1 2737 10 48 50 10 0

Improved IF5_YEAST non-membrane 45232 4.7 2092 26 52 126 19 2

Improved IMB1_YEAST non-membrane 94715 4.3 1288 22 19 146 17 9

Improved KPR3_YEAST non-membrane 35101 8.4 981 12 43 71 12 3

Improved LGUL_YEAST non-membrane 37185 6.4 980 10 39 63 7 1

Improved LSM12_YEAST non-membrane 21300 9.8 1622 13 57 51 10 1

Improved MASZ_YEAST non-membrane 62754 6.0 2662 18 21 87 13 3

Improved MDHC_YEAST non-membrane 40705 6.4 1317 17 32 85 12 2

Improved MMF1_YEAST non-membrane 15898 9.7 35839 26 59 187 13 6

Improved MPD1_YEAST non-membrane 36385 10.0 1770 10 31 56 8 1

Improved MVD1_YEAST non-membrane 44088 5.4 1187 23 57 92 16 2

Improved NAB3_YEAST non-membrane 90383 4.2 1208 19 20 122 17 2

Improved NAT1_YEAST non-membrane 98842 9.0 1049 32 34 137 24 4

Improved NAT2_YEAST non-membrane 32783 10.3 1309 10 26 52 6 0

Improved NIP7_YEAST non-membrane 20367 9.6 2488 12 36 52 6 0

Improved NPT1_YEAST non-membrane 48987 6.5 1057 23 46 118 16 2

Improved NTF2_YEAST non-membrane 14444 4.3 4072 6 67 48 6 2

Improved PCNA_YEAST non-membrane 28897 4.2 981 10 43 58 9 1

Improved PGM1_YEAST non-membrane 63072 6.9 1009 20 28 74 14 2

Improved PHO85_YEAST non-membrane 34883 8.4 1067 13 37 45 10 2

Improved PLR1_YEAST non-membrane 38576 5.6 1121 15 50 86 14 4

Improved PP12_YEAST non-membrane 35884 5.1 1547 13 38 80 10 3

Improved PROB_YEAST non-membrane 47132 6.3 581 21 46 75 18 1

Improved PRS10_YEAST non-membrane 49377 5.4 1363 11 25 60 10 1

Improved PTH2_YEAST non-membrane 22421 5.1 1538 13 48 53 8 1

Improved PYRX_YEAST non-membrane 24795 8.8 1687 10 38 48 8 0

Improved RCC1_YEAST non-membrane 52980 5.7 670 14 42 63 11 0

Improved RCN2_YEAST non-membrane 29237 7.3 1055 19 40 85 11 3

S-10

Improved RIR2_YEAST non-membrane 46118 5.0 1733 21 47 112 15 3

Improved RL11B_YEAST non-membrane 19737 10.3 14300 42 71 214 16 0

Improved RL17A_YEAST non-membrane 20537 11.3 862 22 47 103 15 7

Improved RL19B_YEAST non-membrane 21691 11.8 1763 8 25 34 7 0

Improved RL24A_YEAST non-membrane 17602 11.7 1254 9 42 43 7 1

Improved RL32_YEAST non-membrane 14762 11.6 3078 10 45 53 7 0

Improved RL44B_YEAST non-membrane 12203 11.1 1132 6 23 31 5 1

Improved RLI1_YEAST non-membrane 68297 8.8 1267 33 38 162 19 4

Improved RM24_YEAST non-membrane 30030 10.5 1984 15 46 62 12 2

Improved RPB4_YEAST non-membrane 25398 4.6 1124 10 35 49 7 2

Improved RPC5_YEAST non-membrane 32122 4.2 706 13 35 55 8 1

Improved RPN1_YEAST non-membrane 109423 4.3 804 36 29 173 24 7

Improved RPN3_YEAST non-membrane 60354 5.2 3186 25 44 168 22 5

Improved RS16A_YEAST non-membrane 15837 10.7 77875 45 74 452 25 6

Improved RS19B_YEAST non-membrane 15881 10.0 1911 24 51 108 17 8

Improved RS9A_YEAST non-membrane 22429 10.4 21298 66 78 473 31 4

Improved RT05_YEAST non-membrane 34861 9.9 1035 11 37 54 11 1

Improved RT09_YEAST non-membrane 31905 10.6 3112 18 64 90 12 3

Improved RT23_YEAST non-membrane 50835 10.1 1331 23 41 101 19 1

Improved SCJ1_YEAST non-membrane 41516 7.2 779 20 39 76 12 3

Improved SODM_YEAST non-membrane 25758 8.9 12910 20 42 125 11 1

Improved SRO9_YEAST non-membrane 48030 9.3 1256 13 35 84 11 3

Improved SS120_YEAST non-membrane 27272 4.8 2327 9 25 56 5 0

Improved SYMC_YEAST non-membrane 85624 6.1 1321 36 48 200 32 3

Improved SYV_YEAST non-membrane 125690 6.5 1444 43 33 224 37 7

Improved TCPA_YEAST non-membrane 60442 6.0 1354 24 45 144 19 3

Improved TREA_YEAST non-membrane 85825 7.7 1281 42 49 184 32 2

Improved TSR2_YEAST non-membrane 23698 3.9 1197 8 32 77 7 1

Improved UBA1_YEAST non-membrane 114194 4.8 800 30 34 207 25 7

Improved UBI4P_YEAST non-membrane 42799 7.7 1533 23 46 109 18 2

Improved UPP_YEAST non-membrane 24578 5.4 1112 21 62 101 15 3

Improved XPO1_YEAST non-membrane 124025 5.2 1290 39 40 244 32 9

Improved XRN1_YEAST non-membrane 175349 7.1 1499 80 49 414 65 10

Improved YHI0_YEAST non-membrane 77337 6.0 796 22 28 92 19 4

Improved YIK8_YEAST non-membrane 77365 7.2 702 28 41 103 18 5

Improved YL13B_YEAST non-membrane 198280 7.8 2480 52 26 254 36 9

Improved YP067_YEAST non-membrane 22761 6.9 1717 10 55 68 9 2

Improved YP225_YEAST non-membrane 17433 5.0 3554 4 23 34 4 0

Improved YPT7_YEAST non-membrane 23028 4.6 3226 6 41 53 6 1

S-11

Improved YRF11_YEAST non-membrane 203650 7.3 1715 47 22 194 37 12

Improved YB39B_YEAST unknown 6868 10.3 11218 1 24 15 1 0

Improved YD354_YEAST unknown 5083 4.6 4861 6 89 72 4 2

Improved YIV5_YEAST unknown 27463 5.9 3471 12 48 54 9 3

Improved YNN4_YEAST unknown 41138 5.8 3018 21 49 96 16 2

Default A0A0A7NYF9_YEAST membrane 28548 4.2 2424 4 28 45 4 0

Improved A0A0A7NYF9_YEAST membrane 28548 4.2 4404 15 52 132 11 2

Default A0A0A7P075_YEAST membrane 7754 8.8 5476 9 17 87 5 3

Improved A0A0A7P075_YEAST membrane 7754 8.8 9834 17 17 89 7 3

Default ADT2_YEAST membrane 34404 10.1 32943 65 58 671 37 15

Improved ADT2_YEAST membrane 34404 10.1 97749 136 62 1430 70 39

Default ADT3_YEAST membrane 33291 10.2 3173 19 38 129 15 4

Improved ADT3_YEAST membrane 33291 10.2 16524 73 51 540 53 37

Default AIM24_YEAST membrane 44400 10.2 9098 51 67 452 40 10

Improved AIM24_YEAST membrane 44400 10.2 15722 58 71 381 37 6

Default ALO_YEAST membrane 59455 6.4 3281 25 42 205 21 4

Improved ALO_YEAST membrane 59455 6.4 8225 43 64 362 33 7

Default ATP18_YEAST membrane 6683 10.1 17561 14 71 115 7 2

Improved ATP18_YEAST membrane 6683 10.1 69027 15 80 164 9 2

Default ATP7_YEAST membrane 19797 9.4 32978 58 80 605 29 10

Improved ATP7_YEAST membrane 19797 9.4 40825 67 88 805 39 20

Default ATPA_YEAST membrane 58572 9.4 36789 121 64 1057 56 19

Improved ATPA_YEAST membrane 58572 9.4 44713 147 68 1156 64 23

Default ATPB_YEAST membrane 54760 5.4 21736 86 78 743 54 20

Improved ATPB_YEAST membrane 54760 5.4 42021 102 74 816 52 21

Default ATPD_YEAST membrane 17009 6.2 5419 6 43 74 5 1

Improved ATPD_YEAST membrane 17009 6.2 11663 9 42 82 7 1

Default ATPF_YEAST membrane 26908 9.5 44331 54 51 619 31 14

Improved ATPF_YEAST membrane 26908 9.5 52099 70 59 698 36 18

Default ATPG_YEAST membrane 34328 9.7 9981 46 64 334 24 7

Improved ATPG_YEAST membrane 34328 9.7 11810 58 63 275 28 6

Default ATPJ_YEAST membrane 10868 5.8 22473 18 82 128 10 2

Improved ATPJ_YEAST membrane 10868 5.8 23999 15 56 121 9 2

Default ATPK_YEAST membrane 11304 10.4 22699 23 48 157 10 3

Improved ATPK_YEAST membrane 11304 10.4 37686 14 48 113 9 2

Default ATPN_YEAST membrane 12913 10.1 9359 15 49 83 11 4

Improved ATPN_YEAST membrane 12913 10.1 23112 17 86 170 12 2

Default ATPO_YEAST membrane 22800 10.0 7627 27 70 213 19 2

S-12

Improved ATPO_YEAST membrane 22800 10.0 20964 55 79 381 31 12

Default BFR1_YEAST membrane 54606 9.6 8658 42 57 364 36 7

Improved BFR1_YEAST membrane 54606 9.6 7438 49 52 349 31 11

Default CACP_YEAST membrane 77192 8.3 2789 32 48 206 26 6

Improved CACP_YEAST membrane 77192 8.3 7181 45 39 257 26 5

Default CBP4_YEAST membrane 17521 9.6 2533 10 48 57 8 2

Improved CBP4_YEAST membrane 17521 9.6 2028 15 50 70 9 2

Default CDC12_YEAST membrane 46639 8.1 1222 15 40 77 11 3

Improved CDC12_YEAST membrane 46639 8.1 2181 25 27 102 13 4

Default CDC42_YEAST membrane 21309 6.3 4365 12 46 93 8 2

Improved CDC42_YEAST membrane 21309 6.3 3318 6 34 48 5 2

Default CLH_YEAST membrane 187116 4.9 2111 77 44 625 66 15

Improved CLH_YEAST membrane 187116 4.9 5886 144 66 1022 108 25

Default COA1_YEAST membrane 22030 9.9 2390 14 68 88 10 3

Improved COA1_YEAST membrane 22030 9.9 4064 14 47 90 8 1

Default COPA_YEAST membrane 135522 5.6 1406 54 39 273 40 9

Improved COPA_YEAST membrane 135522 5.6 1871 84 51 419 53 11

Default COPB_YEAST membrane 108951 5.3 690 21 22 135 20 5

Improved COPB_YEAST membrane 108951 5.3 1240 26 29 208 24 5

Default COPB2_YEAST membrane 99383 4.4 2200 43 57 284 35 8

Improved COPB2_YEAST membrane 99383 4.4 1704 42 42 207 29 6

Default COPD_YEAST membrane 60590 4.9 3016 28 38 172 19 5

Improved COPD_YEAST membrane 60590 4.9 2676 24 34 126 15 3

Default COX13_YEAST membrane 15011 8.6 4795 10 64 75 8 1

Improved COX13_YEAST membrane 15011 8.6 13491 13 75 83 7 0

Default COX15_YEAST membrane 54623 10.6 3718 19 23 133 13 1

Improved COX15_YEAST membrane 54623 10.6 7199 33 52 229 24 7

Default COX20_YEAST membrane 23742 9.9 1972 14 47 63 9 2

Improved COX20_YEAST membrane 23742 9.9 1976 19 65 74 13 4

Default COX4_YEAST membrane 17131 6.5 11690 8 65 110 8 3

Improved COX4_YEAST membrane 17131 6.5 13483 12 63 92 9 2

Default COX5A_YEAST membrane 17129 10.2 13155 37 92 257 20 7

Improved COX5A_YEAST membrane 17129 10.2 30003 51 73 347 22 12

Default COX5B_YEAST membrane 17186 10.2 7321 17 60 117 13 4

Improved COX5B_YEAST membrane 17186 10.2 8717 25 33 121 13 5

Default COX6_YEAST membrane 17330 5.7 36382 40 66 372 18 7

Improved COX6_YEAST membrane 17330 5.7 43131 46 63 337 18 8

Default COX9_YEAST membrane 6958 10.2 39809 18 37 105 6 1

Improved COX9_YEAST membrane 6958 10.2 35316 12 37 84 6 1

S-13

Default CRC1_YEAST membrane 34732 10.2 1316 19 60 104 17 3

Improved CRC1_YEAST membrane 34732 10.2 7357 29 62 152 15 3

Default CY1_YEAST membrane 34033 8.2 14845 45 78 353 24 9

Improved CY1_YEAST membrane 34033 8.2 15021 29 47 206 17 6

Default DHSD_YEAST membrane 20235 9.2 6481 5 15 59 4 1

Improved DHSD_YEAST membrane 20235 9.2 15683 12 32 135 8 2

Default DPM1_YEAST membrane 30342 8.0 9505 12 36 101 8 0

Improved DPM1_YEAST membrane 30342 8.0 13859 27 51 214 19 7

Default EMC1_YEAST membrane 87126 5.0 4359 30 44 201 22 5

Improved EMC1_YEAST membrane 87126 5.0 2309 47 63 274 33 7

Default EMC4_YEAST membrane 21445 9.9 3181 12 42 73 9 2

Improved EMC4_YEAST membrane 21445 9.9 2399 8 39 63 7 2

Default EMP24_YEAST membrane 23317 6.3 3650 19 49 108 9 1

Improved EMP24_YEAST membrane 23317 6.3 5901 15 39 106 7 1

Default EMP47_YEAST membrane 50298 5.6 3260 18 37 116 13 2

Improved EMP47_YEAST membrane 50298 5.6 2093 26 36 99 17 3

Default ERG27_YEAST membrane 39699 8.6 1722 13 42 91 12 1

Improved ERG27_YEAST membrane 39699 8.6 2265 18 40 103 12 2

Default ERP1_YEAST membrane 24707 7.2 2022 15 57 79 10 1

Improved ERP1_YEAST membrane 24707 7.2 2829 14 47 99 10 1

Default ERV46_YEAST membrane 46205 6.4 7632 32 64 220 24 8

Improved ERV46_YEAST membrane 46205 6.4 2369 26 50 183 18 6

Default ETFD_YEAST membrane 69589 7.8 2605 33 43 208 24 3

Improved ETFD_YEAST membrane 69589 7.8 2837 38 48 183 24 3

Default FAT2_YEAST membrane 60450 9.5 21976 96 72 781 55 20

Improved FAT2_YEAST membrane 60450 9.5 23484 80 66 600 47 12

Default FDFT_YEAST membrane 51686 5.5 3645 12 25 110 10 1

Improved FDFT_YEAST membrane 51686 5.5 5833 16 28 129 13 0

Default FIS1_YEAST membrane 17722 9.4 7890 12 43 71 7 0

Improved FIS1_YEAST membrane 17722 9.4 9524 14 53 93 11 2

Default FKBP2_YEAST membrane 14477 5.2 5206 8 68 81 7 0

Improved FKBP2_YEAST membrane 14477 5.2 3296 7 67 41 6 0

Default FMP10_YEAST membrane 27680 10.1 5414 24 57 227 20 7

Improved FMP10_YEAST membrane 27680 10.1 4283 26 48 167 19 5

Default FMP52_YEAST membrane 25070 9.6 18740 37 81 324 24 3

Improved FMP52_YEAST membrane 25070 9.6 15082 50 84 361 28 5

Default FSF1_YEAST membrane 35391 10.2 2084 28 49 127 16 5

Improved FSF1_YEAST membrane 35391 10.2 1435 23 58 90 13 3

Default GAS5_YEAST membrane 51837 4.3 1792 10 19 67 8 1

S-14

Improved GAS5_YEAST membrane 51837 4.3 919 20 35 89 12 2

Default GGC1_YEAST membrane 33194 10.4 6151 11 33 84 9 0

Improved GGC1_YEAST membrane 33194 10.4 9408 20 56 140 14 1

Default GPDM_YEAST membrane 72343 7.8 4344 67 61 433 44 10

Improved GPDM_YEAST membrane 72343 7.8 6116 74 69 422 50 9

Default GSF2_YEAST membrane 45840 6.7 5320 35 49 298 22 3

Improved GSF2_YEAST membrane 45840 6.7 5381 36 51 240 25 8

Default GST1_YEAST membrane 26777 6.2 9499 16 48 141 13 0

Improved GST1_YEAST membrane 26777 6.2 7027 20 44 136 17 3

Default GVP36_YEAST membrane 36647 4.7 1452 20 55 122 18 6

Improved GVP36_YEAST membrane 36647 4.7 682 18 44 90 13 6

Default HEMH_YEAST membrane 44567 9.3 5769 26 48 241 21 6

Improved HEMH_YEAST membrane 44567 9.3 9940 50 64 386 27 3

Default HFD1_YEAST membrane 59941 6.3 2045 18 30 133 14 3

Improved HFD1_YEAST membrane 59941 6.3 4911 30 46 222 24 6

Default HSP30_YEAST membrane 37020 4.9 3096 7 20 89 5 1

Improved HSP30_YEAST membrane 37020 4.9 2763 9 25 100 7 2

Default HSP60_YEAST membrane 60714 5.0 3217 25 49 164 23 2

Improved HSP60_YEAST membrane 60714 5.0 2183 41 54 185 33 5

Default HSP77_YEAST membrane 70584 5.3 18137 109 64 1030 68 23

Improved HSP77_YEAST membrane 70584 5.3 22580 117 62 952 66 21

Default HXT6_YEAST membrane 62664 7.7 2787 16 29 165 14 4

Improved HXT6_YEAST membrane 62664 7.7 3910 22 33 216 18 5

Default KAD2_YEAST membrane 24239 6.0 13230 25 80 252 19 5

Improved KAD2_YEAST membrane 24239 6.0 13674 39 88 263 26 8

Default KAPR_YEAST membrane 47190 8.1 2013 16 30 88 12 1

Improved KAPR_YEAST membrane 47190 8.1 3359 29 50 136 19 1

Default KES1_YEAST membrane 49461 5.6 6739 34 67 279 28 3

Improved KES1_YEAST membrane 49461 5.6 6611 35 42 227 19 2

Default KTR1_YEAST membrane 45992 6.2 3749 22 53 140 15 0

Improved KTR1_YEAST membrane 45992 6.2 2884 16 27 85 11 0

Default KTR3_YEAST membrane 47451 8.9 2799 22 39 105 14 2

Improved KTR3_YEAST membrane 47451 8.9 3564 26 41 155 17 1

Default LCB1_YEAST membrane 62167 5.7 1043 14 26 97 11 1

Improved LCB1_YEAST membrane 62167 5.7 1345 15 47 102 14 3

Default MAK11_YEAST membrane 53638 8.5 1346 15 30 92 11 2

Improved MAK11_YEAST membrane 53638 8.5 1009 17 34 66 12 0

Default MAN1_YEAST membrane 124420 6.8 1807 51 44 323 40 7

Improved MAN1_YEAST membrane 124420 6.8 2042 49 34 275 33 2

S-15

Default MCR1_YEAST membrane 34116 9.1 10647 40 79 293 26 5

Improved MCR1_YEAST membrane 34116 9.1 16081 44 67 278 25 4

Default MDJ1_YEAST membrane 55526 9.5 983 23 36 102 18 3

Improved MDJ1_YEAST membrane 55526 9.5 1070 21 34 82 14 3

Default MGLL_YEAST membrane 35539 8.5 2958 25 64 153 19 3

Improved MGLL_YEAST membrane 35539 8.5 4434 43 73 201 24 6

Default MIC10_YEAST membrane 10401 10.7 60054 18 73 131 8 2

Improved MIC10_YEAST membrane 10401 10.7 67931 29 60 171 12 7

Default MNN9_YEAST membrane 45925 6.4 1333 10 23 75 8 0

Improved MNN9_YEAST membrane 45925 6.4 1694 18 29 100 14 3

Default MPCP_YEAST membrane 32791 9.6 32920 33 75 491 27 12

Improved MPCP_YEAST membrane 32791 9.6 53815 75 93 882 41 20

Default MPM1_YEAST membrane 28452 5.5 14170 23 58 264 19 5

Improved MPM1_YEAST membrane 28452 5.5 16222 32 62 293 19 4

Default MSS51_YEAST membrane 50847 8.9 3437 32 52 208 23 5

Improved MSS51_YEAST membrane 50847 8.9 5405 35 49 178 21 1

Default NCB5R_YEAST membrane 31473 9.1 4704 35 69 207 20 2

Improved NCB5R_YEAST membrane 31473 9.1 4135 27 64 125 17 3

Default NCE2_YEAST membrane 18954 9.6 9357 10 20 135 8 5

Improved NCE2_YEAST membrane 18954 9.6 6238 14 32 130 9 5

Default NCPR_YEAST membrane 76723 4.9 2046 34 47 182 25 1

Improved NCPR_YEAST membrane 76723 4.9 2619 44 46 216 29 4

Default NDI1_YEAST membrane 57214 9.8 26168 80 76 732 45 13

Improved NDI1_YEAST membrane 57214 9.8 26892 89 64 615 40 11

Default NUC1_YEAST membrane 37186 9.0 2084 11 38 76 10 2

Improved NUC1_YEAST membrane 37186 9.0 2249 17 53 90 14 1

Default ODC1_YEAST membrane 34184 10.2 3630 27 50 187 19 6

Improved ODC1_YEAST membrane 34184 10.2 12870 38 61 324 27 8

Default OM14_YEAST membrane 14600 8.9 19038 6 40 103 5 1

Improved OM14_YEAST membrane 14600 8.9 48844 17 46 209 11 5

Default OM45_YEAST membrane 44553 9.1 749 17 34 86 15 4

Improved OM45_YEAST membrane 44553 9.1 907 16 27 81 13 3

Default OST1_YEAST membrane 54038 4.8 2600 10 38 93 9 1

Improved OST1_YEAST membrane 54038 4.8 2993 15 47 121 15 2

Default OST3_YEAST membrane 39457 8.6 3386 11 27 99 9 3

Improved OST3_YEAST membrane 39457 8.6 6581 13 27 120 11 3

Default OSTB_YEAST membrane 49361 5.5 6800 34 49 298 21 2

Improved OSTB_YEAST membrane 49361 5.5 7280 34 48 269 22 4

Default OSTD_YEAST membrane 31632 6.6 5525 11 28 121 10 2

S-16

Improved OSTD_YEAST membrane 31632 6.6 4958 8 22 75 7 2

Default PDR16_YEAST membrane 40688 8.1 971 14 38 86 12 2

Improved PDR16_YEAST membrane 40688 8.1 1491 16 39 90 12 2

Default PEX14_YEAST membrane 38396 4.6 987 10 27 58 8 2

Improved PEX14_YEAST membrane 38396 4.6 905 13 36 57 9 2

Default PFKA1_YEAST membrane 107903 5.9 6271 91 69 641 62 12

Improved PFKA1_YEAST membrane 107903 5.9 6798 126 65 661 67 14

Default PFKA2_YEAST membrane 104552 6.2 6463 76 57 536 48 12

Improved PFKA2_YEAST membrane 104552 6.2 5641 62 54 448 41 6

Default PHB1_YEAST membrane 31407 8.9 6595 26 50 185 19 7

Improved PHB1_YEAST membrane 31407 8.9 17521 37 60 268 23 8

Default PHB2_YEAST membrane 34385 10.3 9896 37 62 291 22 3

Improved PHB2_YEAST membrane 34385 10.3 14430 51 52 309 26 7

Default PHO88_YEAST membrane 21122 9.5 8507 41 79 270 23 8

Improved PHO88_YEAST membrane 21122 9.5 14418 29 64 185 14 5

Default PIC2_YEAST membrane 33506 9.6 2321 14 37 131 10 1

Improved PIC2_YEAST membrane 33506 9.6 9866 36 48 262 24 6

Default PIS_YEAST membrane 24807 8.8 4294 10 29 55 6 2

Improved PIS_YEAST membrane 24807 8.8 5020 18 54 96 9 1

Default PMA1_YEAST membrane 99556 4.8 5990 49 40 532 34 10

Improved PMA1_YEAST membrane 99556 4.8 9018 55 43 633 41 11

Default PMA2_YEAST membrane 102107 4.7 1604 34 29 250 26 4

Improved PMA2_YEAST membrane 102107 4.7 5069 40 33 338 29 5

Default PMT2_YEAST membrane 86813 7.5 2362 22 27 224 19 3

Improved PMT2_YEAST membrane 86813 7.5 3602 27 37 314 25 4

Default PROF_YEAST membrane 13668 5.4 4635 8 40 96 7 2

Improved PROF_YEAST membrane 13668 5.4 10688 8 73 63 6 2

Default PYR1_YEAST membrane 244884 5.5 8621 223 68 1632 152 35

Improved PYR1_YEAST membrane 244884 5.5 11476 249 61 1458 139 23

Default QCR1_YEAST membrane 50196 6.9 41834 87 79 985 52 19

Improved QCR1_YEAST membrane 50196 6.9 53259 135 72 1173 60 28

Default QCR10_YEAST membrane 8587 9.3 9678 13 57 68 6 0

Improved QCR10_YEAST membrane 8587 9.3 28479 14 57 96 7 1

Default QCR2_YEAST membrane 40453 8.3 100710 141 88 1437 64 23

Improved QCR2_YEAST membrane 40453 8.3 113507 181 93 1562 70 26

Default QCR7_YEAST membrane 14555 5.5 113035 54 94 558 25 10

Improved QCR7_YEAST membrane 14555 5.5 106538 85 94 686 28 14

Default QCR8_YEAST membrane 10967 10.0 15952 12 54 116 9 4

Improved QCR8_YEAST membrane 10967 10.0 26225 20 77 140 11 3

S-17

Default QCR9_YEAST membrane 7471 5.5 9071 11 35 95 3 0

Improved QCR9_YEAST membrane 7471 5.5 19193 16 29 144 3 0

Default RAS2_YEAST membrane 34684 7.2 2672 12 33 68 9 3

Improved RAS2_YEAST membrane 34684 7.2 3379 20 37 124 16 6

Default RCF1_YEAST membrane 18465 9.5 4653 17 59 188 14 3

Improved RCF1_YEAST membrane 18465 9.5 2261 12 48 78 9 2

Default RCF2_YEAST membrane 25328 9.8 3999 17 53 117 13 1

Improved RCF2_YEAST membrane 25328 9.8 9156 18 78 116 12 2

Default RFS1_YEAST membrane 22906 5.0 968 5 30 39 4 0

Improved RFS1_YEAST membrane 22906 5.0 3101 10 38 53 7 0

Default RHO1_YEAST membrane 23137 5.9 4977 8 30 62 6 0

Improved RHO1_YEAST membrane 23137 5.9 3934 15 45 69 10 1

Default RTN1_YEAST membrane 32895 9.3 5315 12 23 96 8 1

Improved RTN1_YEAST membrane 32895 9.3 12357 25 66 245 18 4

Default SAC1_YEAST membrane 71080 7.5 2862 28 45 216 26 5

Improved SAC1_YEAST membrane 71080 7.5 10401 69 78 528 50 12

Default SAR1_YEAST membrane 21437 5.0 35390 31 81 405 24 10

Improved SAR1_YEAST membrane 21437 5.0 44532 37 74 413 22 9

Default SC160_YEAST membrane 134726 5.5 1832 53 49 312 44 10

Improved SC160_YEAST membrane 134726 5.5 797 49 36 200 37 4

Default SC61A_YEAST membrane 52902 9.6 3571 8 13 70 6 3

Improved SC61A_YEAST membrane 52902 9.6 3358 10 14 83 7 1

Default SC61G_YEAST membrane 8937 9.9 12687 7 23 33 2 0

Improved SC61G_YEAST membrane 8937 9.9 21134 10 41 57 5 1

Default SCS2_YEAST membrane 26908 4.7 5519 15 67 130 14 5

Improved SCS2_YEAST membrane 26908 4.7 6200 18 68 103 11 2

Default SDH3_YEAST membrane 22053 10.6 3909 8 24 77 6 3

Improved SDH3_YEAST membrane 22053 10.6 9584 10 41 128 9 3

Default SDHA_YEAST membrane 70185 7.2 17058 83 73 698 54 14

Improved SDHA_YEAST membrane 70185 7.2 26521 97 67 728 51 13

Default SDHB_YEAST membrane 30211 8.9 13176 33 35 227 15 5

Improved SDHB_YEAST membrane 30211 8.9 12801 46 59 299 22 6

Default SDHX_YEAST membrane 69338 7.0 4442 36 43 219 25 4

Improved SDHX_YEAST membrane 69338 7.0 11621 43 33 265 26 5

Default SEC13_YEAST membrane 33022 5.4 3300 19 64 117 15 1

Improved SEC13_YEAST membrane 33022 5.4 3591 20 45 81 11 0

Default SEC14_YEAST membrane 34878 5.2 7131 32 69 221 22 4

Improved SEC14_YEAST membrane 34878 5.2 7127 39 80 190 23 6

Default SEC17_YEAST membrane 32782 4.8 9475 29 70 293 23 5

S-18

Improved SEC17_YEAST membrane 32782 4.8 6822 24 44 209 16 3

Default SEC23_YEAST membrane 85330 5.3 1788 32 49 251 29 5

Improved SEC23_YEAST membrane 85330 5.3 2676 41 52 318 33 8

Default SEC4_YEAST membrane 23491 7.0 4873 17 59 115 14 1

Improved SEC4_YEAST membrane 23491 7.0 10604 24 67 138 15 4

Default SEC63_YEAST membrane 75297 4.9 2582 24 40 173 20 3

Improved SEC63_YEAST membrane 75297 4.9 4900 29 34 173 21 3

Default SFC1_YEAST membrane 35317 10.1 5720 29 63 207 20 6

Improved SFC1_YEAST membrane 35317 10.1 11899 38 52 187 18 2

Default STE24_YEAST membrane 52290 7.9 585 7 18 37 6 0

Improved STE24_YEAST membrane 52290 7.9 1356 12 22 94 10 0

Default TIM21_YEAST membrane 27188 10.5 1474 10 44 66 9 3

Improved TIM21_YEAST membrane 27188 10.5 2756 10 31 69 8 1

Default TIM44_YEAST membrane 48824 9.8 5013 32 45 230 22 5

Improved TIM44_YEAST membrane 48824 9.8 5747 40 40 200 21 3

Default TIM50_YEAST membrane 55064 6.9 2370 20 32 107 13 1

Improved TIM50_YEAST membrane 55064 6.9 3228 25 35 118 18 2

Default TMEDA_YEAST membrane 24091 5.2 6261 32 81 187 15 3

Improved TMEDA_YEAST membrane 24091 5.2 5574 14 40 92 9 1

Default TOM40_YEAST membrane 42012 5.2 11578 26 66 255 24 7

Improved TOM40_YEAST membrane 42012 5.2 16611 35 48 255 23 9

Default TOM5_YEAST membrane 5981 9.4 11177 6 30 35 3 1

Improved TOM5_YEAST membrane 5981 9.4 11860 4 30 31 2 1

Default TRX1_YEAST membrane 11227 4.6 14290 9 78 100 8 1

Improved TRX1_YEAST membrane 11227 4.6 8963 11 81 77 8 0

Default TRX2_YEAST membrane 11196 4.6 21090 16 79 157 11 2

Improved TRX2_YEAST membrane 11196 4.6 17485 17 93 129 12 2

Default UCRI_YEAST membrane 23349 8.1 12297 16 68 119 11 1

Improved UCRI_YEAST membrane 23349 8.1 22364 32 77 213 17 6

Default VA0D_YEAST membrane 39765 4.3 4070 20 45 192 18 5

Improved VA0D_YEAST membrane 39765 4.3 13505 41 65 342 23 5

Default VAC8_YEAST membrane 63168 4.8 444 8 19 59 8 1

Improved VAC8_YEAST membrane 63168 4.8 2244 17 29 149 15 3

Default VATA_YEAST membrane 118562 5.8 7354 82 58 539 54 5

Improved VATA_YEAST membrane 118562 5.8 7422 103 61 519 63 7

Default VATB_YEAST membrane 57713 4.8 21644 61 80 579 45 12

Improved VATB_YEAST membrane 57713 4.8 23925 77 84 550 42 10

Default VATC_YEAST membrane 44161 6.2 3770 26 48 194 22 3

Improved VATC_YEAST membrane 44161 6.2 3029 46 57 241 27 6

S-19

Default VATE_YEAST membrane 26455 5.1 1341 16 64 83 14 5

Improved VATE_YEAST membrane 26455 5.1 1158 17 59 76 14 2

Default VATF_YEAST membrane 13452 4.8 11464 7 69 67 7 1

Improved VATF_YEAST membrane 13452 4.8 2712 6 48 29 4 0

Default VATH_YEAST membrane 54381 5.9 1639 27 53 198 24 9

Improved VATH_YEAST membrane 54381 5.9 3712 28 46 189 21 3

Default VATL1_YEAST membrane 16339 8.0 1525 2 16 20 2 0

Improved VATL1_YEAST membrane 16339 8.0 936 5 34 40 4 2

Default VDAC1_YEAST membrane 30409 8.2 50093 78 64 723 35 16

Improved VDAC1_YEAST membrane 30409 8.2 78019 98 87 881 42 16

Default VPH1_YEAST membrane 95467 5.1 1565 13 19 116 13 0

Improved VPH1_YEAST membrane 95467 5.1 3691 31 29 301 28 6

Default VPS21_YEAST membrane 23066 5.1 6277 19 65 136 14 3

Improved VPS21_YEAST membrane 23066 5.1 5443 26 72 171 16 1

Default VTC4_YEAST membrane 83103 6.4 1366 31 28 209 21 5

Improved VTC4_YEAST membrane 83103 6.4 1671 36 33 198 26 5

Default YCP4_YEAST membrane 26333 8.4 1999 17 47 122 11 3

Improved YCP4_YEAST membrane 26333 8.4 11144 20 68 124 14 2

Default YD19A_YEAST membrane 7446 11.3 12908 4 36 24 4 0

Improved YD19A_YEAST membrane 7446 11.3 15365 4 61 25 3 0

Default YHM2_YEAST membrane 34162 10.3 8697 36 62 276 25 6

Improved YHM2_YEAST membrane 34162 10.3 9649 34 46 223 19 6

Default YJ55_YEAST membrane 11242 9.6 29118 13 70 156 6 2

Improved YJ55_YEAST membrane 11242 9.6 35997 10 95 116 7 1

Default YKR18_YEAST membrane 81701 4.9 1181 32 33 186 23 7

Improved YKR18_YEAST membrane 81701 4.9 729 26 37 145 23 5

Default YN8B_YEAST membrane 47063 9.7 4495 27 50 196 21 4

Improved YN8B_YEAST membrane 47063 9.7 5025 34 44 225 22 2

Default YO020_YEAST membrane 9611 9.2 19830 10 82 120 9 2

Improved YO020_YEAST membrane 9611 9.2 6479 12 76 115 9 4

Default YOP1_YEAST membrane 20256 9.3 1691 6 13 39 3 0

Improved YOP1_YEAST membrane 20256 9.3 1883 11 16 79 4 0

Default YP010_YEAST membrane 7897 9.3 19956 8 35 80 5 0

Improved YP010_YEAST membrane 7897 9.3 14292 7 90 44 5 0

Default YPT1_YEAST membrane 23199 5.0 3911 19 64 90 11 2

Improved YPT1_YEAST membrane 23199 5.0 2767 12 57 75 10 2

Default YPT31_YEAST membrane 24453 4.8 4090 10 35 61 7 0

Improved YPT31_YEAST membrane 24453 4.8 3901 11 34 76 9 1

Default YPT32_YEAST membrane 24505 5.2 7110 17 50 121 13 1

S-20

Improved YPT32_YEAST membrane 24505 5.2 3281 13 42 69 11 2

Default YPT52_YEAST membrane 26116 4.5 2011 11 41 71 11 2

Improved YPT52_YEAST membrane 26116 4.5 2668 13 45 72 11 0

Default YPT6_YEAST membrane 24397 5.5 876 11 28 50 8 1

Improved YPT6_YEAST membrane 24397 5.5 2257 16 36 57 11 2

Default YRO2_YEAST membrane 38694 9.5 1509 5 24 55 5 1

Improved YRO2_YEAST membrane 38694 9.5 1796 6 17 51 6 1

Default 6PGD1_YEAST non-membrane 53509 6.2 9978 51 63 409 33 5

Improved 6PGD1_YEAST non-membrane 53509 6.2 25356 84 80 797 55 17

Default AATC_YEAST non-membrane 46028 8.5 1516 24 64 123 17 3

Improved AATC_YEAST non-membrane 46028 8.5 1171 24 66 139 20 4

Default ABP1_YEAST non-membrane 65536 4.4 5411 30 39 252 21 5

Improved ABP1_YEAST non-membrane 65536 4.4 6985 45 44 274 28 4

Default ACBP_YEAST non-membrane 10055 4.6 23789 9 77 110 6 2

Improved ACBP_YEAST non-membrane 10055 4.6 19526 10 80 75 7 0

Default ACEA_YEAST non-membrane 62369 6.0 3577 43 39 231 23 2

Improved ACEA_YEAST non-membrane 62369 6.0 4607 49 48 288 32 6

Default ACH1_YEAST non-membrane 58675 6.3 13262 72 80 549 48 11

Improved ACH1_YEAST non-membrane 58675 6.3 16252 86 75 624 51 13

Default ACON_YEAST non-membrane 85314 8.1 3646 46 52 293 35 2

Improved ACON_YEAST non-membrane 85314 8.1 5236 54 48 297 34 2

Default ACS1_YEAST non-membrane 79091 6.1 5253 51 49 368 36 4

Improved ACS1_YEAST non-membrane 79091 6.1 4702 62 52 350 41 7

Default ACS2_YEAST non-membrane 75444 6.2 2133 27 44 201 20 2

Improved ACS2_YEAST non-membrane 75444 6.2 1038 19 28 116 15 0

Default ACT_YEAST non-membrane 41662 5.3 17084 55 66 451 30 9

Improved ACT_YEAST non-membrane 41662 5.3 19406 53 77 371 31 10

Default ADH1_YEAST non-membrane 36825 6.2 49264 83 77 766 37 9

Improved ADH1_YEAST non-membrane 36825 6.2 43696 94 68 674 37 6

Default ADH2_YEAST non-membrane 36708 6.3 11645 61 70 385 33 6

Improved ADH2_YEAST non-membrane 36708 6.3 9021 64 69 334 34 8

Default ADH3_YEAST non-membrane 40344 8.5 3406 24 45 152 15 2

Improved ADH3_YEAST non-membrane 40344 8.5 5637 29 63 145 20 0

Default ADK_YEAST non-membrane 36349 4.8 1505 14 43 101 11 2

Improved ADK_YEAST non-membrane 36349 4.8 1450 21 58 113 16 2

Default AGX1_YEAST non-membrane 41880 8.0 2571 23 65 157 18 2

Improved AGX1_YEAST non-membrane 41880 8.0 2415 23 44 120 15 2

Default AHA1_YEAST non-membrane 39411 7.3 2236 25 58 136 15 1

Improved AHA1_YEAST non-membrane 39411 7.3 2034 27 44 142 18 4

S-21

Default AHP1_YEAST non-membrane 19102 4.8 8758 11 85 121 9 0

Improved AHP1_YEAST non-membrane 19102 4.8 7310 14 69 107 9 0

Default ALDH4_YEAST non-membrane 56688 6.3 5336 48 64 380 32 5

Improved ALDH4_YEAST non-membrane 56688 6.3 12970 54 48 348 32 3

Default ALDH6_YEAST non-membrane 54379 5.1 5536 49 61 311 32 4

Improved ALDH6_YEAST non-membrane 54379 5.1 8323 44 53 278 27 4

Default ALF_YEAST non-membrane 39595 5.4 54248 54 56 523 27 8

Improved ALF_YEAST non-membrane 39595 5.4 63827 85 60 702 35 14

Default AMPL_YEAST non-membrane 57057 5.5 2071 25 47 150 19 3

Improved AMPL_YEAST non-membrane 57057 5.5 1945 26 44 127 18 2

Default APD1_YEAST non-membrane 35736 6.8 2123 20 48 127 15 1

Improved APD1_YEAST non-membrane 35736 6.8 1823 17 45 88 11 1

Default APE3_YEAST non-membrane 60099 5.0 2448 24 42 189 19 4

Improved APE3_YEAST non-membrane 60099 5.0 2953 22 30 129 15 2

Default ARB1_YEAST non-membrane 68334 6.1 3077 41 46 208 27 4

Improved ARB1_YEAST non-membrane 68334 6.1 2169 48 55 225 31 5

Default ARC1_YEAST non-membrane 42057 8.0 2906 23 55 158 16 3

Improved ARC1_YEAST non-membrane 42057 8.0 6931 33 66 277 25 4

Default ARD1_YEAST non-membrane 27585 4.6 3358 11 39 52 8 1

Improved ARD1_YEAST non-membrane 27585 4.6 2876 7 33 45 6 1

Default ARF1_YEAST non-membrane 20516 7.3 47410 35 90 493 27 10

Improved ARF1_YEAST non-membrane 20516 7.3 62168 53 71 514 27 14

Default ARF2_YEAST non-membrane 20644 7.3 33290 32 62 385 25 11

Improved ARF2_YEAST non-membrane 20644 7.3 42117 60 67 516 34 18

Default ARL1_YEAST non-membrane 20421 4.6 3429 14 60 82 11 1

Improved ARL1_YEAST non-membrane 20421 4.6 2759 12 63 49 9 1

Default ARO8_YEAST non-membrane 56142 5.6 3609 27 43 173 19 1

Improved ARO8_YEAST non-membrane 56142 5.6 8153 54 63 312 32 8

Default AROG_YEAST non-membrane 39724 6.5 1039 12 41 76 10 0

Improved AROG_YEAST non-membrane 39724 6.5 3080 20 39 117 12 3

Default ARP2_YEAST non-membrane 44045 5.4 1731 16 48 113 13 0

Improved ARP2_YEAST non-membrane 44045 5.4 873 16 47 69 12 2

Default ARP3_YEAST non-membrane 49510 5.4 1123 25 64 147 17 2

Improved ARP3_YEAST non-membrane 49510 5.4 2414 25 51 150 21 2

Default ARPC2_YEAST non-membrane 39541 6.4 2697 11 32 127 10 2

Improved ARPC2_YEAST non-membrane 39541 6.4 3156 19 50 103 13 1

Default ARPC3_YEAST non-membrane 20565 8.2 3230 9 35 78 8 1

Improved ARPC3_YEAST non-membrane 20565 8.2 8838 22 83 145 16 2

Default ARPC4_YEAST non-membrane 19903 5.1 9754 14 70 126 9 1

S-22

Improved ARPC4_YEAST non-membrane 19903 5.1 7457 22 56 151 12 2

Default AYR1_YEAST non-membrane 32793 9.5 2867 22 59 159 17 3

Improved AYR1_YEAST non-membrane 32793 9.5 6578 43 85 225 26 5

Default BBC1_YEAST non-membrane 128218 5.0 3811 53 23 448 37 11

Improved BBC1_YEAST non-membrane 128218 5.0 4442 49 26 338 32 7

Default BCA1_YEAST non-membrane 43568 9.2 4062 27 52 164 19 3

Improved BCA1_YEAST non-membrane 43568 9.2 3134 26 64 152 19 3

Default BCA2_YEAST non-membrane 41598 7.1 2558 25 63 141 18 3

Improved BCA2_YEAST non-membrane 41598 7.1 2545 21 50 114 14 3

Default BMH1_YEAST non-membrane 30072 4.6 15434 27 60 247 16 3

Improved BMH1_YEAST non-membrane 30072 4.6 11006 35 61 219 17 2

Default BMH2_YEAST non-membrane 31042 4.6 14539 40 64 294 24 6

Improved BMH2_YEAST non-membrane 31042 4.6 12514 47 66 298 24 4

Default C1TC_YEAST non-membrane 102141 6.4 2447 78 69 434 57 16

Improved C1TC_YEAST non-membrane 102141 6.4 2316 39 46 240 30 5

Default C1TM_YEAST non-membrane 106151 8.9 1023 41 47 221 36 8

Improved C1TM_YEAST non-membrane 106151 8.9 839 35 39 143 27 6

Default CALM_YEAST non-membrane 16124 4.0 3711 9 88 67 9 1

Improved CALM_YEAST non-membrane 16124 4.0 1494 7 50 37 5 1

Default CARB_YEAST non-membrane 123837 5.0 1532 46 48 245 37 6

Improved CARB_YEAST non-membrane 123837 5.0 1378 40 35 182 29 3

Default CARP_YEAST non-membrane 44471 4.5 13392 52 34 433 24 10

Improved CARP_YEAST non-membrane 44471 4.5 13229 51 49 361 28 11

Default CBF5_YEAST non-membrane 54670 9.2 1964 19 42 110 15 3

Improved CBF5_YEAST non-membrane 54670 9.2 1365 19 39 78 14 1

Default CBS_YEAST non-membrane 55987 6.2 6371 63 75 410 43 10

Improved CBS_YEAST non-membrane 55987 6.2 9983 64 67 362 39 7

Default CDC48_YEAST non-membrane 91938 4.6 9585 43 44 346 31 2

Improved CDC48_YEAST non-membrane 91938 4.6 6774 44 43 285 31 2

Default CH10_YEAST non-membrane 11365 9.6 7687 9 70 62 7 1

Improved CH10_YEAST non-membrane 11365 9.6 4656 8 75 55 7 0

Default CISY1_YEAST non-membrane 53327 8.6 26258 74 69 618 32 7

Improved CISY1_YEAST non-membrane 53327 8.6 44931 118 82 767 45 11

Default CISY2_YEAST non-membrane 51381 5.9 7051 38 56 312 28 8

Improved CISY2_YEAST non-membrane 51381 5.9 12999 52 72 343 32 7

Default CISY3_YEAST non-membrane 53776 8.7 1023 19 49 92 16 0

Improved CISY3_YEAST non-membrane 53776 8.7 1771 23 34 104 16 3

Default CLU_YEAST non-membrane 145075 6.0 2893 40 36 308 33 6

Improved CLU_YEAST non-membrane 145075 6.0 3373 56 41 310 44 6

S-23

Default COFI_YEAST non-membrane 15890 4.9 37824 25 70 206 12 2

Improved COFI_YEAST non-membrane 15890 4.9 37547 25 87 194 14 3

Default CRP1_YEAST non-membrane 51084 7.3 1150 18 48 79 13 1

Improved CRP1_YEAST non-membrane 51084 7.3 1936 17 32 73 12 2

Default CYPC_YEAST non-membrane 19906 9.1 20458 26 81 232 16 1

Improved CYPC_YEAST non-membrane 19906 9.1 18270 34 60 217 16 3

Default CYPD_YEAST non-membrane 25310 5.2 8585 17 50 171 13 3

Improved CYPD_YEAST non-membrane 25310 5.2 7656 21 59 152 15 4

Default CYPH_YEAST non-membrane 17379 7.3 50274 43 64 408 18 5

Improved CYPH_YEAST non-membrane 17379 7.3 54304 35 81 295 15 5

Default DAK1_YEAST non-membrane 62167 5.1 2648 25 42 161 21 5

Improved DAK1_YEAST non-membrane 62167 5.1 2232 40 53 171 25 1

Default DED1_YEAST non-membrane 65512 7.8 5961 42 64 298 37 6

Improved DED1_YEAST non-membrane 65512 7.8 6556 43 46 219 25 5

Default DHE2_YEAST non-membrane 124254 5.4 4043 59 41 396 39 11

Improved DHE2_YEAST non-membrane 124254 5.4 4037 82 50 433 47 7

Default DHE4_YEAST non-membrane 49538 5.4 2272 23 44 120 17 0

Improved DHE4_YEAST non-membrane 49538 5.4 1611 26 52 121 18 2

Default DHOM_YEAST non-membrane 38478 7.6 15105 46 65 505 34 11

Improved DHOM_YEAST non-membrane 38478 7.6 40925 70 83 768 39 13

Default DLDH_YEAST non-membrane 53976 8.1 49288 127 82 1282 70 25

Improved DLDH_YEAST non-membrane 53976 8.1 38123 133 84 1018 65 19

Default ECM14_YEAST non-membrane 49797 5.0 1401 17 56 118 14 3

Improved ECM14_YEAST non-membrane 49797 5.0 1592 14 30 60 9 2

Default EF1A_YEAST non-membrane 50001 9.4 31080 92 66 802 42 13

Improved EF1A_YEAST non-membrane 50001 9.4 32836 142 77 946 51 11

Default EF1B_YEAST non-membrane 22613 4.1 16222 32 76 370 19 7

Improved EF1B_YEAST non-membrane 22613 4.1 23677 17 49 130 9 1

Default EF1G1_YEAST non-membrane 47058 8.7 11149 59 74 479 39 13

Improved EF1G1_YEAST non-membrane 47058 8.7 18616 71 75 441 44 12

Default EF1G2_YEAST non-membrane 46490 8.1 20215 55 72 517 31 12

Improved EF1G2_YEAST non-membrane 46490 8.1 28610 70 66 590 39 13

Default EF2_YEAST non-membrane 93230 5.9 24549 102 71 1365 75 32

Improved EF2_YEAST non-membrane 93230 5.9 31190 162 78 1702 96 32

Default EF3A_YEAST non-membrane 115871 5.7 4156 75 57 565 59 18

Improved EF3A_YEAST non-membrane 115871 5.7 4942 74 52 449 47 8

Default EF3B_YEAST non-membrane 115795 5.8 1155 51 48 232 39 10

Improved EF3B_YEAST non-membrane 115795 5.8 976 32 30 151 26 2

Default EFTU_YEAST non-membrane 47941 6.2 3041 24 46 163 19 3

S-24

Improved EFTU_YEAST non-membrane 47941 6.2 2019 25 42 144 18 0

Default EIF2A_YEAST non-membrane 71260 9.2 1520 21 39 141 18 3

Improved EIF2A_YEAST non-membrane 71260 9.2 865 29 36 142 18 6

Default EIF3A_YEAST non-membrane 110276 5.9 2405 52 43 257 37 4

Improved EIF3A_YEAST non-membrane 110276 5.9 2321 61 43 259 38 3

Default EIF3B_YEAST non-membrane 88074 5.6 4074 57 56 401 42 6

Improved EIF3B_YEAST non-membrane 88074 5.6 3816 69 53 362 40 5

Default EIF3C_YEAST non-membrane 93147 4.7 2051 34 34 255 28 8

Improved EIF3C_YEAST non-membrane 93147 4.7 2825 37 41 286 32 10

Default EIF3I_YEAST non-membrane 38731 5.3 2589 28 54 166 18 3

Improved EIF3I_YEAST non-membrane 38731 5.3 2012 20 54 115 15 0

Default EIF3J_YEAST non-membrane 29545 4.8 2687 10 31 65 9 0

Improved EIF3J_YEAST non-membrane 29545 4.8 6290 23 50 121 16 2

Default EMC2_YEAST non-membrane 33833 5.4 1318 7 27 44 5 1

Improved EMC2_YEAST non-membrane 33833 5.4 3815 26 48 140 16 3

Default ENO1_YEAST non-membrane 46787 6.2 47359 84 74 827 40 10

Improved ENO1_YEAST non-membrane 46787 6.2 57573 103 84 839 47 15

Default ENO2_YEAST non-membrane 46885 5.6 63474 114 81 1112 54 18

Improved ENO2_YEAST non-membrane 46885 5.6 65763 141 85 1196 50 19

Default ERF1_YEAST non-membrane 48975 4.7 3935 28 58 192 22 1

Improved ERF1_YEAST non-membrane 48975 4.7 2341 24 54 142 20 3

Default ERG6_YEAST non-membrane 43403 5.4 9445 41 54 284 23 4

Improved ERG6_YEAST non-membrane 43403 5.4 13292 54 49 312 27 8

Default ETR1_YEAST non-membrane 42040 9.4 3673 21 39 172 16 3

Improved ETR1_YEAST non-membrane 42040 9.4 4265 33 48 197 18 1

Default F16P_YEAST non-membrane 38238 5.6 5346 30 45 258 19 6

Improved F16P_YEAST non-membrane 38238 5.6 15621 44 81 449 37 11

Default FAS1_YEAST non-membrane 228545 5.5 3309 113 50 826 81 16

Improved FAS1_YEAST non-membrane 228545 5.5 4914 120 42 653 70 15

Default FAS2_YEAST non-membrane 206816 5.2 3218 132 55 812 94 16

Improved FAS2_YEAST non-membrane 206816 5.2 4339 115 46 611 74 22

Default FBRL_YEAST non-membrane 34443 10.6 11334 36 51 233 23 6

Improved FBRL_YEAST non-membrane 34443 10.6 20957 43 65 309 24 4

Default FHP_YEAST non-membrane 44618 5.8 2944 34 64 192 22 4

Improved FHP_YEAST non-membrane 44618 5.8 2212 25 59 163 20 5

Default FIMB_YEAST non-membrane 71728 5.1 4492 28 35 211 24 10

Improved FIMB_YEAST non-membrane 71728 5.1 9552 73 54 552 47 17

Default FKBP_YEAST non-membrane 12150 5.6 25671 20 96 180 14 5

Improved FKBP_YEAST non-membrane 12150 5.6 25897 23 68 178 13 4

S-25

Default FMP46_YEAST non-membrane 15640 9.7 5511 9 59 75 7 0

Improved FMP46_YEAST non-membrane 15640 9.7 5345 13 58 59 8 2

Default FOX2_YEAST non-membrane 98641 9.3 3465 63 56 341 45 8

Improved FOX2_YEAST non-membrane 98641 9.3 6784 85 70 505 59 8

Default FPPS_YEAST non-membrane 40457 5.2 5439 18 51 177 16 3

Improved FPPS_YEAST non-membrane 40457 5.2 6442 32 57 228 27 8

Default G3P1_YEAST non-membrane 35727 8.5 59589 108 87 989 47 14

Improved G3P1_YEAST non-membrane 35727 8.5 64570 130 80 1041 55 19

Default G3P2_YEAST non-membrane 35824 6.5 53355 85 76 925 50 17

Improved G3P2_YEAST non-membrane 35824 6.5 66690 141 76 1060 61 26

Default G3P3_YEAST non-membrane 35724 6.5 70749 87 80 1075 48 16

Improved G3P3_YEAST non-membrane 35724 6.5 100666 152 80 1438 69 26

Default G6PI_YEAST non-membrane 61261 6.0 14025 49 51 464 28 7

Improved G6PI_YEAST non-membrane 61261 6.0 29691 87 67 929 49 17

Default GAR1_YEAST non-membrane 21467 11.9 2366 5 20 44 4 0

Improved GAR1_YEAST non-membrane 21467 11.9 4087 9 22 45 5 1

Default GBLP_YEAST non-membrane 34783 5.8 42578 83 94 742 34 13

Improved GBLP_YEAST non-membrane 34783 5.8 52418 91 94 824 39 18

Default GCY1_YEAST non-membrane 35057 8.2 705 15 53 84 14 1

Improved GCY1_YEAST non-membrane 35057 8.2 2053 21 55 115 16 1

Default GDI1_YEAST non-membrane 51174 5.6 3546 30 47 202 20 3

Improved GDI1_YEAST non-membrane 51174 5.6 8446 52 72 341 33 5

Default GDIR_YEAST non-membrane 23124 5.7 6548 16 73 108 14 2

Improved GDIR_YEAST non-membrane 23124 5.7 4838 8 48 71 7 0

Default GFA1_YEAST non-membrane 79996 6.0 6021 32 46 230 26 2

Improved GFA1_YEAST non-membrane 79996 6.0 2138 25 38 134 18 1

Default GLNA_YEAST non-membrane 41739 5.9 4263 29 50 177 19 3

Improved GLNA_YEAST non-membrane 41739 5.9 4593 20 30 104 10 0

Default GLRX1_YEAST non-membrane 12372 4.8 23967 18 69 149 13 5

Improved GLRX1_YEAST non-membrane 12372 4.8 14065 14 93 100 10 2

Default GLRX2_YEAST non-membrane 15851 7.2 4623 7 42 63 5 0

Improved GLRX2_YEAST non-membrane 15851 7.2 3411 10 51 73 8 2

Default GLYC_YEAST non-membrane 52185 7.1 4526 20 41 122 15 2

Improved GLYC_YEAST non-membrane 52185 7.1 8992 52 71 295 32 5

Default GPG1_YEAST non-membrane 14912 4.5 10815 8 52 57 6 1

Improved GPG1_YEAST non-membrane 14912 4.5 13071 5 14 78 2 0

Default GPP1_YEAST non-membrane 27929 5.2 10125 13 46 121 11 0

Improved GPP1_YEAST non-membrane 27929 5.2 8605 18 66 146 15 1

Default GPP2_YEAST non-membrane 27796 5.7 2758 11 36 57 9 1

S-26

Improved GPP2_YEAST non-membrane 27796 5.7 4007 7 30 53 6 0

Default GPX3_YEAST non-membrane 18629 8.4 8925 13 54 145 11 1

Improved GPX3_YEAST non-membrane 18629 8.4 12939 18 40 148 11 2

Default GRP78_YEAST non-membrane 74422 4.6 4968 58 43 316 34 8

Improved GRP78_YEAST non-membrane 74422 4.6 5219 65 40 353 35 7

Default GSHR_YEAST non-membrane 53407 7.8 2111 18 51 127 16 3

Improved GSHR_YEAST non-membrane 53407 7.8 2829 28 43 151 21 1

Default GSP1_YEAST non-membrane 24794 6.1 6803 19 55 141 15 3

Improved GSP1_YEAST non-membrane 24794 6.1 10212 25 47 142 12 1

Default GSP2_YEAST non-membrane 24974 6.2 6825 19 63 116 14 1

Improved GSP2_YEAST non-membrane 24974 6.2 8312 20 53 123 13 3

Default H2A1_YEAST non-membrane 13980 11.1 4298 15 68 121 12 3

Improved H2A1_YEAST non-membrane 13980 11.1 9301 23 39 206 13 7

Default H2B1_YEAST non-membrane 14243 10.5 25679 31 60 239 17 5

Improved H2B1_YEAST non-membrane 14243 10.5 5180 27 57 144 13 3

Default H2B2_YEAST non-membrane 14228 10.5 31748 31 69 288 18 6

Improved H2B2_YEAST non-membrane 14228 10.5 52158 39 69 420 21 8

Default H3_YEAST non-membrane 15346 11.8 10378 12 46 111 6 0

Improved H3_YEAST non-membrane 15346 11.8 6859 13 24 74 5 0

Default H4_YEAST non-membrane 11361 11.8 66026 30 50 305 15 5

Improved H4_YEAST non-membrane 11361 11.8 88118 40 65 333 15 2

Default HCH1_YEAST non-membrane 17235 4.4 3756 12 53 67 10 0

Improved HCH1_YEAST non-membrane 17235 4.4 3232 13 58 75 8 0

Default HMCS_YEAST non-membrane 54978 8.2 2044 21 46 124 16 3

Improved HMCS_YEAST non-membrane 54978 8.2 2910 23 41 115 14 1

Default HS104_YEAST non-membrane 101972 5.1 13733 120 61 829 65 15

Improved HS104_YEAST non-membrane 101972 5.1 13875 125 49 748 62 14

Default HSC82_YEAST non-membrane 80849 4.6 32493 150 78 1398 72 19

Improved HSC82_YEAST non-membrane 80849 4.6 28986 139 64 985 65 15

Default HSP26_YEAST non-membrane 23865 5.2 29627 33 74 350 20 7

Improved HSP26_YEAST non-membrane 23865 5.2 21767 43 57 319 20 8

Default HSP71_YEAST non-membrane 69614 4.8 29541 198 75 1677 123 50

Improved HSP71_YEAST non-membrane 69614 4.8 29070 183 72 1202 90 41

Default HSP72_YEAST non-membrane 69427 4.7 37203 167 78 1721 97 39

Improved HSP72_YEAST non-membrane 69427 4.7 35958 194 82 1567 108 51

Default HSP73_YEAST non-membrane 70503 4.9 12185 67 46 468 46 12

Improved HSP73_YEAST non-membrane 70503 4.9 16395 73 40 414 40 15

Default HSP74_YEAST non-membrane 69608 4.8 12233 124 59 868 74 26

Improved HSP74_YEAST non-membrane 69608 4.8 8752 133 62 720 66 20

S-27

Default HSP75_YEAST non-membrane 66560 5.1 30622 135 82 958 75 27

Improved HSP75_YEAST non-membrane 66560 5.1 29014 128 75 954 65 20

Default HSP76_YEAST non-membrane 66553 5.2 38421 121 85 1067 62 21

Improved HSP76_YEAST non-membrane 66553 5.2 39642 142 73 1030 67 22

Default HSP78_YEAST non-membrane 91279 8.1 3911 38 37 287 28 4

Improved HSP78_YEAST non-membrane 91279 8.1 4891 79 53 483 48 7

Default HSP79_YEAST non-membrane 77572 5.3 757 33 38 147 25 2

Improved HSP79_YEAST non-membrane 77572 5.3 1712 31 30 126 19 3

Default HSP7E_YEAST non-membrane 70041 5.9 3197 53 39 315 33 12

Improved HSP7E_YEAST non-membrane 70041 5.9 2291 43 52 253 30 9

Default HSP7F_YEAST non-membrane 77318 4.9 5448 45 58 366 34 2

Improved HSP7F_YEAST non-membrane 77318 4.9 7265 56 48 406 37 3

Default HSP82_YEAST non-membrane 81356 4.6 23397 144 70 1117 78 22

Improved HSP82_YEAST non-membrane 81356 4.6 19238 153 60 908 68 19

Default HXKA_YEAST non-membrane 53704 5.1 10371 62 75 474 43 12

Improved HXKA_YEAST non-membrane 53704 5.1 17204 81 61 495 41 10

Default HXKB_YEAST non-membrane 53908 5.0 12039 49 72 443 33 8

Improved HXKB_YEAST non-membrane 53908 5.0 16059 79 76 686 48 18

Default HXKG_YEAST non-membrane 55342 5.8 931 30 46 130 22 4

Improved HXKG_YEAST non-membrane 55342 5.8 3343 30 49 158 23 5

Default IDH1_YEAST non-membrane 39299 9.4 19765 47 62 372 22 3

Improved IDH1_YEAST non-membrane 39299 9.4 22267 67 59 437 28 5

Default IDH2_YEAST non-membrane 39714 9.0 7424 22 36 157 14 1

Improved IDH2_YEAST non-membrane 39714 9.0 6619 25 48 173 16 0

Default IDHC_YEAST non-membrane 46532 5.8 21384 51 64 459 31 7

Improved IDHC_YEAST non-membrane 46532 5.8 23270 92 72 588 48 11

Default IDHH_YEAST non-membrane 47826 9.6 5543 27 41 143 18 1

Improved IDHH_YEAST non-membrane 47826 9.6 7512 31 38 132 19 1

Default IDHP_YEAST non-membrane 48160 9.1 9523 37 64 288 27 4

Improved IDHP_YEAST non-membrane 48160 9.1 11883 76 76 382 38 6

Default IF2A_YEAST non-membrane 34695 4.7 5274 19 52 132 16 3

Improved IF2A_YEAST non-membrane 34695 4.7 5385 28 61 175 19 2

Default IF2B_YEAST non-membrane 31554 9.9 2333 19 51 93 14 1

Improved IF2B_YEAST non-membrane 31554 9.9 2258 21 47 122 16 2

Default IF2G_YEAST non-membrane 57829 6.4 3404 37 51 254 29 5

Improved IF2G_YEAST non-membrane 57829 6.4 4468 32 58 196 26 4

Default IF2P_YEAST non-membrane 112199 5.5 945 23 22 180 22 5

Improved IF2P_YEAST non-membrane 112199 5.5 1939 46 36 315 39 9

Default IF4A_YEAST non-membrane 44669 4.8 23429 82 87 681 36 11

S-28

Improved IF4A_YEAST non-membrane 44669 4.8 20856 106 83 600 47 22

Default IF4E_YEAST non-membrane 24239 5.1 12258 19 57 174 13 2

Improved IF4E_YEAST non-membrane 24239 5.1 14138 33 68 245 19 4

Default IF4F1_YEAST non-membrane 107036 5.7 1164 26 29 181 23 8

Improved IF4F1_YEAST non-membrane 107036 5.7 1117 17 19 113 17 7

Default IF5A1_YEAST non-membrane 17103 4.6 7564 10 41 81 7 1

Improved IF5A1_YEAST non-membrane 17103 4.6 9654 11 43 52 6 1

Default IF5A2_YEAST non-membrane 17120 4.6 1571 6 38 34 5 0

Improved IF5A2_YEAST non-membrane 17120 4.6 1437 4 29 17 4 0

Default IF6_YEAST non-membrane 26441 4.3 3223 11 31 67 7 1

Improved IF6_YEAST non-membrane 26441 4.3 1761 13 43 75 10 2

Default ILV3_YEAST non-membrane 62820 7.7 1848 20 43 126 15 3

Improved ILV3_YEAST non-membrane 62820 7.7 991 44 66 164 33 8

Default ILV5_YEAST non-membrane 44340 9.3 16691 53 67 494 38 13

Improved ILV5_YEAST non-membrane 44340 9.3 44962 96 62 704 39 13

Default ILV6_YEAST non-membrane 33965 6.1 3923 28 63 132 18 3

Improved ILV6_YEAST non-membrane 33965 6.1 1676 19 56 101 12 3

Default ILVB_YEAST non-membrane 74889 8.8 1814 28 36 181 23 4

Improved ILVB_YEAST non-membrane 74889 8.8 2301 37 40 215 25 5

Default IMB4_YEAST non-membrane 122524 4.3 1163 40 35 245 34 9

Improved IMB4_YEAST non-membrane 122524 4.3 7380 48 40 523 42 11

Default IMDH1_YEAST non-membrane 44357 8.2 3639 38 59 249 30 17

Improved IMDH1_YEAST non-membrane 44357 8.2 5646 27 50 125 15 3

Default IMDH2_YEAST non-membrane 56493 8.6 4545 39 38 259 30 11

Improved IMDH2_YEAST non-membrane 56493 8.6 5772 41 56 178 25 6

Default IMDH3_YEAST non-membrane 56548 7.2 7288 52 54 464 38 16

Improved IMDH3_YEAST non-membrane 56548 7.2 7558 39 54 258 26 5

Default IMDH4_YEAST non-membrane 56357 8.2 4577 40 39 260 28 7

Improved IMDH4_YEAST non-membrane 56357 8.2 9873 37 50 310 26 6

Default IMG1_YEAST non-membrane 19381 10.8 1275 7 42 31 6 0

Improved IMG1_YEAST non-membrane 19381 10.8 2095 9 49 44 8 1

Default IPB2_YEAST non-membrane 8584 6.3 1869 7 45 40 4 1

Improved IPB2_YEAST non-membrane 8584 6.3 1448 4 33 14 3 0

Default IPYR_YEAST non-membrane 32279 5.2 19166 36 69 334 22 6

Improved IPYR_YEAST non-membrane 32279 5.2 11555 44 73 267 22 6

Default ITPA_YEAST non-membrane 22079 5.3 2401 6 23 37 4 0

Improved ITPA_YEAST non-membrane 22079 5.3 980 8 27 45 4 0

Default KAR_YEAST non-membrane 35538 5.8 5314 37 63 220 21 5

Improved KAR_YEAST non-membrane 35538 5.8 10293 45 88 248 29 4

S-29

Default KCY_YEAST non-membrane 22918 6.2 1777 11 60 57 10 0

Improved KCY_YEAST non-membrane 22918 6.2 2883 19 46 79 13 4

Default KGUA_YEAST non-membrane 20624 7.2 9476 19 79 163 13 0

Improved KGUA_YEAST non-membrane 20624 7.2 10165 13 63 114 10 2

Default KPYK1_YEAST non-membrane 54510 7.6 61915 155 80 1551 77 28

Improved KPYK1_YEAST non-membrane 54510 7.6 63581 183 82 1337 70 24

Default KPYK2_YEAST non-membrane 55160 6.5 799 23 40 118 15 3

Improved KPYK2_YEAST non-membrane 55160 6.5 963 12 18 66 8 1

Default LCF1_YEAST non-membrane 77817 7.4 2849 38 50 248 28 2

Improved LCF1_YEAST non-membrane 77817 7.4 3987 44 52 329 35 9

Default LEU1_YEAST non-membrane 68366 5.6 2976 39 49 186 25 2

Improved LEU1_YEAST non-membrane 68366 5.6 2086 34 42 141 17 3

Default LSB3_YEAST non-membrane 49314 8.3 1640 22 40 122 16 3

Improved LSB3_YEAST non-membrane 49314 8.3 1153 23 44 110 18 4

Default LSM6_YEAST non-membrane 9391 5.6 2324 4 44 21 3 0

Improved LSM6_YEAST non-membrane 9391 5.6 5639 11 94 57 9 1

Default LSP1_YEAST non-membrane 38047 4.4 10708 38 47 293 21 6

Improved LSP1_YEAST non-membrane 38047 4.4 12117 39 42 261 20 7

Default LYS1_YEAST non-membrane 41438 9.4 10779 38 54 304 24 1

Improved LYS1_YEAST non-membrane 41438 9.4 18775 39 69 328 20 0

Default MAP1_YEAST non-membrane 43345 7.8 652 7 37 38 7 1

Improved MAP1_YEAST non-membrane 43345 7.8 827 11 35 58 9 1

Default MAP2_YEAST non-membrane 47488 5.8 1261 14 32 77 9 1

Improved MAP2_YEAST non-membrane 47488 5.8 2922 20 30 78 11 2

Default MAS5_YEAST non-membrane 44642 5.9 3037 19 43 148 16 1

Improved MAS5_YEAST non-membrane 44642 5.9 4056 16 35 111 13 1

Default MASY_YEAST non-membrane 62751 6.8 3320 34 36 213 19 5

Improved MASY_YEAST non-membrane 62751 6.8 8185 50 67 329 35 6

Default MCFS2_YEAST non-membrane 51223 7.7 4911 36 51 259 25 3

Improved MCFS2_YEAST non-membrane 51223 7.7 4980 40 60 208 26 3

Default MDHM_YEAST non-membrane 35627 9.0 49673 71 81 632 40 14

Improved MDHM_YEAST non-membrane 35627 9.0 54887 81 77 643 36 13

Default MDHP_YEAST non-membrane 37162 9.6 14016 37 68 331 25 6

Improved MDHP_YEAST non-membrane 37162 9.6 38487 76 78 749 43 17

Default MDY2_YEAST non-membrane 23717 9.7 2362 8 54 48 7 0

Improved MDY2_YEAST non-membrane 23717 9.7 1841 21 67 94 16 3

Default METE_YEAST non-membrane 85806 6.0 4117 63 62 416 47 8

Improved METE_YEAST non-membrane 85806 6.0 3784 71 61 365 45 9

Default METK1_YEAST non-membrane 41792 4.9 3503 27 45 161 16 2

S-30

Improved METK1_YEAST non-membrane 41792 4.9 2918 41 62 186 24 3

Default METK2_YEAST non-membrane 42229 5.0 788 7 25 41 6 0

Improved METK2_YEAST non-membrane 42229 5.0 2017 38 61 141 21 2

Default MG101_YEAST non-membrane 30071 9.7 1168 16 50 74 12 1

Improved MG101_YEAST non-membrane 30071 9.7 1603 18 50 83 12 0

Default MNP1_YEAST non-membrane 20637 9.7 6479 14 27 105 7 0

Improved MNP1_YEAST non-membrane 20637 9.7 8406 14 45 84 9 1

Default MPG1_YEAST non-membrane 39541 5.9 15729 54 66 452 31 6

Improved MPG1_YEAST non-membrane 39541 5.9 25962 59 74 450 33 7

Default MRT4_YEAST non-membrane 27041 8.9 10745 28 74 213 21 2

Improved MRT4_YEAST non-membrane 27041 8.9 14425 36 73 218 20 4

Default NACB1_YEAST non-membrane 17009 6.1 3542 9 61 70 7 1

Improved NACB1_YEAST non-membrane 17009 6.1 3976 16 46 87 7 1

Default NDH1_YEAST non-membrane 62734 9.6 22080 91 68 810 55 15

Improved NDH1_YEAST non-membrane 62734 9.6 17157 83 62 555 44 8

Default NDH2_YEAST non-membrane 61620 9.0 1047 26 44 171 21 5

Improved NDH2_YEAST non-membrane 61620 9.0 2049 37 60 246 29 8

Default NDK_YEAST non-membrane 17155 9.1 38527 17 66 175 12 2

Improved NDK_YEAST non-membrane 17155 9.1 35174 35 76 268 15 3

Default NNRE_YEAST non-membrane 27503 8.4 1112 11 47 50 7 0

Improved NNRE_YEAST non-membrane 27503 8.4 2186 18 55 89 13 3

Default NOP3_YEAST non-membrane 45379 5.2 8331 14 34 154 12 1

Improved NOP3_YEAST non-membrane 45379 5.2 4296 7 17 76 6 1

Default NOP56_YEAST non-membrane 56828 9.3 1994 33 52 183 24 5

Improved NOP56_YEAST non-membrane 56828 9.3 2307 34 53 141 23 3

Default NOP58_YEAST non-membrane 56921 9.4 4861 28 49 242 22 6

Improved NOP58_YEAST non-membrane 56921 9.4 8966 49 47 371 27 6

Default ODO1_YEAST non-membrane 114345 6.8 8398 85 49 697 53 9

Improved ODO1_YEAST non-membrane 114345 6.8 11267 108 62 836 72 17

Default ODO2_YEAST non-membrane 50399 9.3 16875 54 45 415 28 11

Improved ODO2_YEAST non-membrane 50399 9.3 17312 56 38 381 26 9

Default ODP2_YEAST non-membrane 51785 7.9 3067 26 38 164 18 2

Improved ODP2_YEAST non-membrane 51785 7.9 4398 39 50 223 26 6

Default ODPA_YEAST non-membrane 46313 8.1 2666 19 40 124 15 2

Improved ODPA_YEAST non-membrane 46313 8.1 4665 31 54 158 21 3

Default ODPB_YEAST non-membrane 40028 5.0 8492 25 42 173 16 2

Improved ODPB_YEAST non-membrane 40028 5.0 9014 18 39 137 12 0

Default OLA1_YEAST non-membrane 44146 7.2 1201 12 35 77 12 3

Improved OLA1_YEAST non-membrane 44146 7.2 2366 26 57 119 17 0

S-31

Default OSH7_YEAST non-membrane 49773 6.8 1094 15 46 93 12 4

Improved OSH7_YEAST non-membrane 49773 6.8 654 20 33 100 16 2

Default OYE2_YEAST non-membrane 44982 6.1 13636 33 68 244 24 4

Improved OYE2_YEAST non-membrane 44982 6.1 10970 38 50 250 23 8

Default P5CR_YEAST non-membrane 30112 5.2 3999 14 42 94 9 1

Default PAA1_YEAST non-membrane 21933 5.5 7968 26 77 179 21 6

Improved PAA1_YEAST non-membrane 21933 5.5 16231 27 76 164 16 5

Default PABP_YEAST non-membrane 64304 5.6 11424 47 48 415 39 6

Improved PABP_YEAST non-membrane 64304 5.6 9875 49 73 397 36 5

Default PCKA_YEAST non-membrane 60944 5.9 27915 68 59 773 44 17

Improved PCKA_YEAST non-membrane 60944 5.9 26895 92 75 808 54 17

Default PDC1_YEAST non-membrane 61456 5.7 46278 130 65 1539 66 36

Improved PDC1_YEAST non-membrane 61456 5.7 38544 168 68 1160 50 24

Default PDC5_YEAST non-membrane 61873 6.0 10629 40 43 350 30 17

Improved PDC5_YEAST non-membrane 61873 6.0 7071 40 41 265 28 17

Default PDC6_YEAST non-membrane 61542 5.8 10602 44 29 341 28 16

Improved PDC6_YEAST non-membrane 61542 5.8 7852 37 48 202 24 10

Default PDI_YEAST non-membrane 58190 4.2 5637 57 79 388 37 8

Improved PDI_YEAST non-membrane 58190 4.2 5413 47 64 281 30 4

Default PDX3_YEAST non-membrane 26891 7.5 7057 12 70 110 12 0

Improved PDX3_YEAST non-membrane 26891 7.5 3512 15 50 90 11 1

Default PGK_YEAST non-membrane 44710 7.6 100832 176 84 1775 65 19

Improved PGK_YEAST non-membrane 44710 7.6 89318 159 89 1544 58 17

Default PHSG_YEAST non-membrane 103210 5.3 2936 64 47 342 42 9

Improved PHSG_YEAST non-membrane 103210 5.3 2753 67 52 333 40 8

Default PIL1_YEAST non-membrane 38326 4.3 17183 47 62 444 28 10

Improved PIL1_YEAST non-membrane 38326 4.3 17701 44 47 342 25 7

Default PMG1_YEAST non-membrane 27591 9.3 59475 85 87 798 40 12

Improved PMG1_YEAST non-membrane 27591 9.3 48448 127 85 833 37 13

Default PMM_YEAST non-membrane 29044 5.0 3518 18 53 137 13 3

Improved PMM_YEAST non-membrane 29044 5.0 5867 33 62 190 19 0

Default PNC1_YEAST non-membrane 24977 5.8 7475 9 45 92 8 1

Improved PNC1_YEAST non-membrane 24977 5.8 6396 15 54 109 9 1

Default POB3_YEAST non-membrane 62955 4.5 2623 14 24 101 11 0

Improved POB3_YEAST non-membrane 62955 4.5 985 18 28 80 13 3

Default PPID_YEAST non-membrane 42045 5.8 8427 50 42 301 24 9

Improved PPID_YEAST non-membrane 42045 5.8 16361 51 46 341 28 8

Default PROA_YEAST non-membrane 49709 5.2 1540 30 59 183 25 8

Improved PROA_YEAST non-membrane 49709 5.2 1196 28 46 142 21 4

S-32

Default PRPD_YEAST non-membrane 57647 9.4 2255 33 50 222 23 5

Improved PRPD_YEAST non-membrane 57647 9.4 12928 58 60 441 29 7

Default PRS6B_YEAST non-membrane 47864 5.2 7591 41 61 264 29 5

Improved PRS6B_YEAST non-membrane 47864 5.2 7549 44 79 265 30 8

Default PRTB_YEAST non-membrane 69578 5.9 3353 15 18 118 10 2

Improved PRTB_YEAST non-membrane 69578 5.9 2387 31 26 186 18 6

Default PRX1_YEAST non-membrane 29477 9.2 14372 21 61 174 14 0

Improved PRX1_YEAST non-membrane 29477 9.2 17410 26 74 179 15 2

Default PST2_YEAST non-membrane 20952 5.4 882 5 62 35 5 1

Improved PST2_YEAST non-membrane 20952 5.4 6526 12 46 75 7 1

Default PTE1_YEAST non-membrane 40234 9.0 2766 12 36 83 11 1

Improved PTE1_YEAST non-membrane 40234 9.0 3193 18 37 88 11 0

Default PUB1_YEAST non-membrane 50731 4.8 1160 12 29 84 9 1

Improved PUB1_YEAST non-membrane 50731 4.8 1631 7 20 42 6 0

Default PUR2_YEAST non-membrane 86014 4.9 889 26 48 164 25 4

Improved PUR2_YEAST non-membrane 86014 4.9 1409 37 52 215 28 5

Default PUR7_YEAST non-membrane 34582 5.6 1602 16 46 110 13 0

Improved PUR7_YEAST non-membrane 34582 5.6 1634 21 41 87 13 0

Default PYC1_YEAST non-membrane 130017 5.8 5634 113 53 851 73 20

Improved PYC1_YEAST non-membrane 130017 5.8 7061 105 52 638 65 18

Default PYC2_YEAST non-membrane 130085 6.0 3470 115 54 716 84 28

Improved PYC2_YEAST non-membrane 130085 6.0 3575 105 54 470 70 26

Default PYRE_YEAST non-membrane 24649 5.7 1786 11 45 67 10 2

Improved PYRE_YEAST non-membrane 24649 5.7 4614 16 60 107 13 4

Default QOR_YEAST non-membrane 36995 9.0 8941 24 57 185 20 3

Improved QOR_YEAST non-membrane 36995 9.0 11731 36 70 267 29 2

Default REE1_YEAST non-membrane 21953 5.9 7465 22 87 169 19 1

Improved REE1_YEAST non-membrane 21953 5.9 10918 16 78 117 12 0

Default RIB4_YEAST non-membrane 18543 6.1 2957 15 73 86 7 1

Improved RIB4_YEAST non-membrane 18543 6.1 5619 14 47 93 8 2

Default RIM1_YEAST non-membrane 15376 9.0 6454 11 50 104 9 2

Improved RIM1_YEAST non-membrane 15376 9.0 7154 17 84 118 12 3

Default RIR4_YEAST non-membrane 40028 4.9 8575 30 66 314 23 1

Improved RIR4_YEAST non-membrane 40028 4.9 16728 62 77 509 40 10

Default RL10_YEAST non-membrane 25345 10.4 25071 47 74 433 30 5

Improved RL10_YEAST non-membrane 25345 10.4 26010 35 73 370 25 5

Default RL11A_YEAST non-membrane 19707 10.3 17447 40 44 234 17 6

Improved RL11A_YEAST non-membrane 19707 10.3 8499 32 55 176 18 5

Default RL12A_YEAST non-membrane 17811 9.9 4738 8 34 61 6 1

S-33

Improved RL12A_YEAST non-membrane 17811 9.9 5710 11 56 84 10 1

Default RL12B_YEAST non-membrane 17811 9.9 25669 24 61 219 15 2

Improved RL12B_YEAST non-membrane 17811 9.9 35083 39 72 316 17 2

Default RL14A_YEAST non-membrane 15157 11.4 43312 51 70 504 30 12

Improved RL14A_YEAST non-membrane 15157 11.4 26286 42 77 390 23 6

Default RL14B_YEAST non-membrane 15143 11.4 31603 42 69 281 21 6

Improved RL14B_YEAST non-membrane 15143 11.4 38362 52 68 324 27 6

Default RL15A_YEAST non-membrane 24407 11.8 10301 21 51 144 14 4

Improved RL15A_YEAST non-membrane 24407 11.8 9645 27 37 151 16 8

Default RL15B_YEAST non-membrane 24407 11.8 8037 18 43 136 14 4

Improved RL15B_YEAST non-membrane 24407 11.8 8580 31 51 151 14 2

Default RL16A_YEAST non-membrane 22187 10.9 7823 34 50 228 21 3

Improved RL16A_YEAST non-membrane 22187 10.9 15038 52 65 318 26 1

Default RL16B_YEAST non-membrane 22235 11.0 16241 37 65 275 22 2

Improved RL16B_YEAST non-membrane 22235 11.0 24697 64 70 364 26 2

Default RL18A_YEAST non-membrane 20550 12.1 12922 17 35 121 8 1

Improved RL18A_YEAST non-membrane 20550 12.1 43633 52 49 372 19 5

Default RL18B_YEAST non-membrane 20550 12.1 33466 54 62 414 27 8

Improved RL18B_YEAST non-membrane 20550 12.1 12801 34 55 173 14 2

Default RL19A_YEAST non-membrane 21691 11.8 4864 16 32 96 11 3

Improved RL19A_YEAST non-membrane 21691 11.8 4161 16 39 83 13 3

Default RL1A_YEAST non-membrane 24470 10.1 33666 61 80 817 40 21

Improved RL1A_YEAST non-membrane 24470 10.1 67096 100 68 1090 55 27

Default RL1B_YEAST non-membrane 24470 10.1 10381 33 48 277 21 6

Improved RL1B_YEAST non-membrane 24470 10.1 9556 38 54 233 20 5

Default RL21A_YEAST non-membrane 18230 10.8 10067 16 53 148 11 2

Improved RL21A_YEAST non-membrane 18230 10.8 15538 24 51 166 14 4

Default RL21B_YEAST non-membrane 18262 10.8 5312 12 37 93 8 2

Improved RL21B_YEAST non-membrane 18262 10.8 7950 28 43 168 11 3

Default RL22A_YEAST non-membrane 13685 5.8 40483 12 32 161 4 2

Improved RL22A_YEAST non-membrane 13685 5.8 67620 19 69 318 11 5

Default RL22B_YEAST non-membrane 13818 5.8 7108 11 67 68 7 1

Improved RL22B_YEAST non-membrane 13818 5.8 5551 8 61 57 4 0

Default RL23A_YEAST non-membrane 14463 10.8 4804 14 62 88 10 0

Improved RL23A_YEAST non-membrane 14463 10.8 8830 20 74 96 13 2

Default RL23B_YEAST non-membrane 14463 10.8 5670 12 74 54 7 1

Improved RL23B_YEAST non-membrane 14463 10.8 8851 27 74 141 16 6

Default RL25_YEAST non-membrane 15747 10.5 2073 8 35 40 5 1

Improved RL25_YEAST non-membrane 15747 10.5 2617 12 54 63 9 0

S-34

Default RL28_YEAST non-membrane 16711 11.0 8415 11 42 71 9 2

Improved RL28_YEAST non-membrane 16711 11.0 23284 21 56 124 12 1

Default RL2A_YEAST non-membrane 27391 11.5 2922 20 57 154 14 1

Improved RL2A_YEAST non-membrane 27391 11.5 2947 22 54 111 17 1

Default RL3_YEAST non-membrane 43730 10.7 20847 94 71 779 47 11

Improved RL3_YEAST non-membrane 43730 10.7 33367 120 80 882 51 9

Default RL30_YEAST non-membrane 11408 10.2 88085 68 86 595 24 11

Improved RL30_YEAST non-membrane 11408 10.2 52470 44 67 306 14 6

Default RL35A_YEAST non-membrane 13901 11.0 3255 14 48 86 9 2

Improved RL35A_YEAST non-membrane 13901 11.0 5671 12 43 78 7 0

Default RL43A_YEAST non-membrane 10084 10.8 25985 17 67 128 10 0

Improved RL43A_YEAST non-membrane 10084 10.8 13534 12 65 64 7 0

Default RL43B_YEAST non-membrane 10084 10.8 10238 6 60 59 6 0

Improved RL43B_YEAST non-membrane 10084 10.8 35417 12 60 92 8 0

Default RL4A_YEAST non-membrane 39068 11.1 46237 84 80 905 49 22

Improved RL4A_YEAST non-membrane 39068 11.1 48508 98 78 737 45 17

Default RL4B_YEAST non-membrane 39038 11.1 35028 81 65 800 58 30

Improved RL4B_YEAST non-membrane 39038 11.1 66263 113 78 992 59 27

Default RL4P_YEAST non-membrane 31949 10.0 2875 28 53 146 19 3

Improved RL4P_YEAST non-membrane 31949 10.0 3309 19 60 111 14 1

Default RL5_YEAST non-membrane 33694 6.4 39319 57 77 606 29 8

Improved RL5_YEAST non-membrane 33694 6.4 40662 85 78 610 35 9

Default RL6A_YEAST non-membrane 19949 10.5 5946 13 40 106 9 0

Improved RL6A_YEAST non-membrane 19949 10.5 8743 19 54 98 11 1

Default RL6B_YEAST non-membrane 19974 10.5 3866 16 34 85 10 1

Improved RL6B_YEAST non-membrane 19974 10.5 12919 18 67 137 13 1

Default RL7A_YEAST non-membrane 27621 10.5 38254 69 68 603 30 7

Improved RL7A_YEAST non-membrane 27621 10.5 59330 88 75 697 41 15

Default RL7B_YEAST non-membrane 27679 10.5 26291 75 69 574 41 15

Improved RL7B_YEAST non-membrane 27679 10.5 74937 103 75 1045 63 32

Default RL8A_YEAST non-membrane 28107 10.5 20641 69 65 504 33 9

Improved RL8A_YEAST non-membrane 28107 10.5 42075 136 71 853 52 18

Default RL8B_YEAST non-membrane 28094 10.4 31583 62 68 558 32 10

Improved RL8B_YEAST non-membrane 28094 10.4 66331 134 71 916 48 19

Default RL9A_YEAST non-membrane 21555 10.1 35530 33 70 308 21 9

Improved RL9A_YEAST non-membrane 21555 10.1 35765 42 63 286 18 4

Default RL9B_YEAST non-membrane 21643 10.0 19565 30 66 199 21 8

Improved RL9B_YEAST non-membrane 21643 10.0 25814 37 59 184 15 2

Default RLA0_YEAST non-membrane 33696 4.5 18461 39 45 447 22 7

S-35

Improved RLA0_YEAST non-membrane 33696 4.5 47086 56 54 582 29 9

Default RLA1_YEAST non-membrane 10901 3.5 22765 4 51 119 4 1

Improved RLA1_YEAST non-membrane 10901 3.5 24557 9 51 220 8 5

Default RLA2_YEAST non-membrane 10739 3.7 68820 19 55 260 8 2

Improved RLA2_YEAST non-membrane 10739 3.7 76467 22 55 214 9 4

Default RLA3_YEAST non-membrane 10661 3.6 23646 8 69 100 5 3

Improved RLA3_YEAST non-membrane 10661 3.6 22427 7 20 75 4 3

Default RLA4_YEAST non-membrane 11043 3.9 64683 22 56 289 12 5

Improved RLA4_YEAST non-membrane 11043 3.9 52248 16 88 139 9 2

Default RM01_YEAST non-membrane 30976 10.4 2758 14 61 89 13 2

Improved RM01_YEAST non-membrane 30976 10.4 2610 19 59 99 15 1

Default RM02_YEAST non-membrane 43200 10.2 1955 15 42 88 15 2

Improved RM02_YEAST non-membrane 43200 10.2 1312 24 36 90 14 0

Default RM03_YEAST non-membrane 43971 9.7 1471 24 41 101 17 4

Improved RM03_YEAST non-membrane 43971 9.7 2055 15 37 93 13 0

Default RM17_YEAST non-membrane 32192 9.5 1777 17 42 85 11 1

Improved RM17_YEAST non-membrane 32192 9.5 5140 24 60 142 19 2

Default RM35_YEAST non-membrane 42798 9.8 2387 27 63 155 18 3

Improved RM35_YEAST non-membrane 42798 9.8 3586 31 66 177 19 4

Default RPAC1_YEAST non-membrane 37663 5.2 1612 14 28 70 11 2

Improved RPAC1_YEAST non-membrane 37663 5.2 1327 20 42 104 14 1

Default RPN11_YEAST non-membrane 34376 5.8 1476 17 49 73 11 2

Improved RPN11_YEAST non-membrane 34376 5.8 1523 9 29 58 8 1

Default RPN13_YEAST non-membrane 17890 4.3 17119 10 63 86 8 1

Improved RPN13_YEAST non-membrane 17890 4.3 15246 10 53 78 7 1

Default RPN2_YEAST non-membrane 104166 5.8 2103 39 29 271 30 2

Improved RPN2_YEAST non-membrane 104166 5.8 4991 62 51 405 48 5

Default RPN5_YEAST non-membrane 51736 5.7 1287 26 47 129 17 2

Improved RPN5_YEAST non-membrane 51736 5.7 784 18 38 69 13 0

Default RPN6_YEAST non-membrane 49742 5.8 1005 11 31 78 9 1

Improved RPN6_YEAST non-membrane 49742 5.8 2100 22 43 122 18 4

Default RPN8_YEAST non-membrane 38288 5.3 1032 8 36 50 7 1

Improved RPN8_YEAST non-membrane 38288 5.3 1795 19 61 98 14 3

Default RPN9_YEAST non-membrane 45753 5.4 3134 21 37 153 19 0

Improved RPN9_YEAST non-membrane 45753 5.4 6503 41 56 252 26 2

Default RS10A_YEAST non-membrane 12731 9.2 22245 48 70 411 25 12

Improved RS10A_YEAST non-membrane 12731 9.2 29330 44 58 330 17 8

Default RS10B_YEAST non-membrane 12730 9.4 15366 52 59 442 31 18

Improved RS10B_YEAST non-membrane 12730 9.4 25685 25 78 183 12 3

S-36

Default RS11A_YEAST non-membrane 17737 11.2 15341 20 58 134 14 1

Improved RS11A_YEAST non-membrane 17737 11.2 14614 23 61 127 15 3

Default RS11B_YEAST non-membrane 17737 11.2 9991 18 50 145 13 4

Improved RS11B_YEAST non-membrane 17737 11.2 9008 20 56 130 12 2

Default RS12_YEAST non-membrane 15462 4.5 6032 18 53 130 9 1

Improved RS12_YEAST non-membrane 15462 4.5 23063 14 50 154 11 4

Default RS13_YEAST non-membrane 17018 10.8 35748 45 71 415 20 7

Improved RS13_YEAST non-membrane 17018 10.8 89644 70 69 563 20 8

Default RS14A_YEAST non-membrane 14527 11.2 11916 13 71 115 11 1

Improved RS14A_YEAST non-membrane 14527 11.2 14357 26 72 131 13 2

Default RS14B_YEAST non-membrane 14640 11.0 14620 14 74 146 11 0

Improved RS14B_YEAST non-membrane 14640 11.0 9269 22 54 118 12 2

Default RS15_YEAST non-membrane 15991 11.1 16325 14 48 154 10 4

Improved RS15_YEAST non-membrane 15991 11.1 17388 14 48 123 11 4

Default RS16B_YEAST non-membrane 15837 10.7 39224 33 76 352 22 3

Improved RS16B_YEAST non-membrane 15837 10.7 36331 33 73 422 22 6

Default RS17B_YEAST non-membrane 15793 10.9 9017 12 51 67 7 0

Improved RS17B_YEAST non-membrane 15793 10.9 6992 19 65 112 13 0

Default RS18A_YEAST non-membrane 17027 10.7 36339 36 69 282 19 2

Improved RS18A_YEAST non-membrane 17027 10.7 17953 31 71 183 19 4

Default RS18B_YEAST non-membrane 17027 10.7 32027 39 66 269 21 3

Improved RS18B_YEAST non-membrane 17027 10.7 40791 43 70 279 24 6

Default RS2_YEAST non-membrane 27432 10.9 39969 51 56 459 25 8

Improved RS2_YEAST non-membrane 27432 10.9 49872 58 65 521 26 10

Default RS22A_YEAST non-membrane 14616 10.3 25744 24 53 220 12 5

Improved RS22A_YEAST non-membrane 14616 10.3 31968 52 91 319 23 10

Default RS22B_YEAST non-membrane 14616 10.3 19411 19 45 136 10 3

Improved RS22B_YEAST non-membrane 14616 10.3 33379 33 48 280 15 8

Default RS23A_YEAST non-membrane 16027 11.2 1842 15 51 56 8 2

Improved RS23A_YEAST non-membrane 16027 11.2 13130 13 57 101 8 0

Default RS23B_YEAST non-membrane 16027 11.2 6810 17 65 127 14 1

Improved RS23B_YEAST non-membrane 16027 11.2 6304 19 40 89 9 0

Default RS26A_YEAST non-membrane 13496 11.2 6007 5 35 42 4 0

Improved RS26A_YEAST non-membrane 13496 11.2 6907 6 39 49 5 1

Default RS27_YEAST non-membrane 8873 9.5 3510 9 48 38 5 0

Improved RS27_YEAST non-membrane 8873 9.5 4565 8 68 44 4 1

Default RS27B_YEAST non-membrane 8859 9.5 6433 7 46 52 4 0

Improved RS27B_YEAST non-membrane 8859 9.5 1790 2 29 11 2 0

Default RS3_YEAST non-membrane 26486 9.8 90405 85 85 915 38 14

S-37

Improved RS3_YEAST non-membrane 26486 9.8 83874 101 86 835 38 14

Default RS3A1_YEAST non-membrane 28725 10.5 22427 65 62 392 35 8

Improved RS3A1_YEAST non-membrane 28725 10.5 26064 92 73 549 38 11

Default RS3A2_YEAST non-membrane 28794 10.5 36194 66 61 509 33 8

Improved RS3A2_YEAST non-membrane 28794 10.5 40925 98 73 697 46 15

Default RS4A_YEAST non-membrane 29392 10.5 7394 30 55 218 19 0

Improved RS4A_YEAST non-membrane 29392 10.5 35484 57 70 438 27 1

Default RS4B_YEAST non-membrane 29392 10.5 4383 14 44 95 12 0

Improved RS4B_YEAST non-membrane 29392 10.5 22983 55 70 333 26 1

Default RS5_YEAST non-membrane 25023 9.1 14031 50 48 296 19 7

Improved RS5_YEAST non-membrane 25023 9.1 27571 68 54 442 28 10

Default RS6A_YEAST non-membrane 26979 10.9 18426 33 50 242 20 3

Improved RS6A_YEAST non-membrane 26979 10.9 23963 65 60 415 32 6

Default RS6B_YEAST non-membrane 26979 10.9 37761 41 62 495 32 10

Improved RS6B_YEAST non-membrane 26979 10.9 13979 37 53 214 20 7

Default RS7A_YEAST non-membrane 21608 10.3 47522 45 85 430 31 10

Improved RS7A_YEAST non-membrane 21608 10.3 64875 49 57 350 21 3

Default RS7B_YEAST non-membrane 21620 10.4 35790 43 79 349 21 2

Default RS8A_YEAST non-membrane 22475 11.1 4897 15 43 97 10 2

Improved RS8A_YEAST non-membrane 22475 11.1 13784 15 52 147 12 1

Default RS8B_YEAST non-membrane 22475 11.1 13942 22 58 198 15 4

Improved RS8B_YEAST non-membrane 22475 11.1 16081 27 52 184 12 1

Default RS9B_YEAST non-membrane 22285 10.5 23437 53 73 487 31 5

Improved RS9B_YEAST non-membrane 22285 10.5 28105 62 68 399 26 5

Default RSSA1_YEAST non-membrane 28007 4.4 16910 11 36 123 8 3

Improved RSSA1_YEAST non-membrane 28007 4.4 22060 34 41 238 14 1

Default RSSA2_YEAST non-membrane 27945 4.5 21565 26 60 214 16 5

Improved RSSA2_YEAST non-membrane 27945 4.5 18748 33 58 211 14 2

Default RT04_YEAST non-membrane 44125 9.1 1715 18 54 104 15 1

Improved RT04_YEAST non-membrane 44125 9.1 3336 22 51 141 15 2

Default RT06_YEAST non-membrane 15011 10.2 5794 5 24 46 5 1

Improved RT06_YEAST non-membrane 15011 10.2 6547 7 45 39 5 0

Default RT07_YEAST non-membrane 27798 10.1 1921 17 47 77 13 3

Improved RT07_YEAST non-membrane 27798 10.1 2110 25 56 101 14 1

Default RT10_YEAST non-membrane 23409 9.6 2566 15 65 92 13 1

Improved RT10_YEAST non-membrane 23409 9.6 5893 16 52 84 9 1

Default RT18_YEAST non-membrane 24547 10.3 1835 8 48 45 7 1

Improved RT18_YEAST non-membrane 24547 10.3 857 9 42 44 8 1

Default RUVB1_YEAST non-membrane 50421 5.5 1689 19 48 113 17 3

S-38

Improved RUVB1_YEAST non-membrane 50421 5.5 1293 20 49 116 18 3

Default RUVB2_YEAST non-membrane 51579 5.0 3029 28 54 145 21 2

Improved RUVB2_YEAST non-membrane 51579 5.0 3105 27 46 132 20 3

Default RV167_YEAST non-membrane 52741 5.7 1842 15 31 150 14 6

Improved RV167_YEAST non-membrane 52741 5.7 3306 26 37 176 17 4

Default SAHH_YEAST non-membrane 49094 5.8 3150 21 37 123 16 3

Improved SAHH_YEAST non-membrane 49094 5.8 2073 18 33 92 13 2

Default SDS22_YEAST non-membrane 38863 5.2 837 14 48 95 11 4

Improved SDS22_YEAST non-membrane 38863 5.2 1190 18 50 99 13 3

Default SER33_YEAST non-membrane 51156 6.0 2582 19 55 106 16 1

Improved SER33_YEAST non-membrane 51156 6.0 1397 20 31 102 14 2

Default SIS1_YEAST non-membrane 37566 9.4 12372 36 60 334 27 7

Improved SIS1_YEAST non-membrane 37566 9.4 14074 35 58 280 23 4

Default SNU13_YEAST non-membrane 13560 8.0 8052 10 75 91 8 3

Improved SNU13_YEAST non-membrane 13560 8.0 14033 13 56 137 9 4

Default SPS19_YEAST non-membrane 31089 9.4 9116 19 49 169 17 2

Improved SPS19_YEAST non-membrane 31089 9.4 16477 34 65 234 22 5

Default SPT16_YEAST non-membrane 118556 4.8 2851 47 35 265 32 5

Improved SPT16_YEAST non-membrane 118556 4.8 3364 60 39 373 42 3

Default SSZ1_YEAST non-membrane 58202 4.8 8576 68 80 464 41 10

Improved SSZ1_YEAST non-membrane 58202 4.8 14147 55 70 433 32 5

Default STI1_YEAST non-membrane 66224 5.3 2754 41 49 279 34 7

Improved STI1_YEAST non-membrane 66224 5.3 3216 53 59 288 37 6

Default SUB2_YEAST non-membrane 50277 5.2 2050 22 48 114 15 2

Improved SUB2_YEAST non-membrane 50277 5.2 2264 38 51 190 22 2

Default SUCA_YEAST non-membrane 35010 8.7 3340 22 63 153 16 6

Improved SUCA_YEAST non-membrane 35010 8.7 3699 32 58 142 19 4

Default SUCB_YEAST non-membrane 46871 7.3 1122 18 48 88 17 0

Improved SUCB_YEAST non-membrane 46871 7.3 2326 16 35 79 12 3

Default SYA_YEAST non-membrane 109991 5.4 1855 49 42 208 35 5

Improved SYA_YEAST non-membrane 109991 5.4 2088 68 49 279 48 3

Default SYDC_YEAST non-membrane 63476 6.1 5846 42 44 282 26 3

Improved SYDC_YEAST non-membrane 63476 6.1 6227 48 63 286 39 4

Default SYEC_YEAST non-membrane 80791 7.3 9421 51 51 374 37 5

Improved SYEC_YEAST non-membrane 80791 7.3 9907 51 38 327 36 3

Default SYG_YEAST non-membrane 78135 6.1 6522 57 56 386 39 7

Improved SYG_YEAST non-membrane 78135 6.1 7215 73 59 399 43 6

Default SYH_YEAST non-membrane 59915 7.7 982 18 46 110 16 4

Improved SYH_YEAST non-membrane 59915 7.7 2786 26 38 116 14 0

S-39

Default SYIC_YEAST non-membrane 122906 5.7 2448 56 47 381 48 6

Improved SYIC_YEAST non-membrane 122906 5.7 9054 80 57 597 60 7

Default SYLC_YEAST non-membrane 124062 5.5 1278 34 33 162 27 4

Improved SYLC_YEAST non-membrane 124062 5.5 2534 58 38 212 39 6

Default SYNC_YEAST non-membrane 62167 5.5 1865 23 49 132 17 1

Improved SYNC_YEAST non-membrane 62167 5.5 5623 43 47 281 31 7

Default SYQ_YEAST non-membrane 93074 8.5 1027 42 37 251 34 5

Improved SYQ_YEAST non-membrane 93074 8.5 1162 42 40 186 32 6

Default SYRC_YEAST non-membrane 69480 6.3 2130 46 51 233 34 4

Improved SYRC_YEAST non-membrane 69480 6.3 2594 40 56 147 29 3

Default SYTC_YEAST non-membrane 84466 6.6 857 29 34 138 25 0

Improved SYTC_YEAST non-membrane 84466 6.6 998 32 31 131 22 1

Default TAL1_YEAST non-membrane 37013 6.0 5472 37 57 217 24 5

Improved TAL1_YEAST non-membrane 37013 6.0 5178 33 53 162 19 3

Default TBA1_YEAST non-membrane 49768 4.8 1539 9 30 47 8 1

Improved TBA1_YEAST non-membrane 49768 4.8 1962 7 28 45 6 0

Default TBA3_YEAST non-membrane 49662 4.9 1734 10 40 65 10 2

Improved TBA3_YEAST non-membrane 49662 4.9 1418 8 27 42 7 0

Default TBB_YEAST non-membrane 50890 4.4 1122 14 35 103 14 2

Improved TBB_YEAST non-membrane 50890 4.4 2616 27 49 124 16 4

Default TCTP_YEAST non-membrane 18729 4.2 13614 15 37 138 9 1

Improved TCTP_YEAST non-membrane 18729 4.2 8693 15 44 128 10 2

Default THIL_YEAST non-membrane 41702 7.2 1321 16 40 109 13 3

Improved THIL_YEAST non-membrane 41702 7.2 3733 31 58 169 20 6

Default THRC_YEAST non-membrane 57438 5.3 2206 21 46 148 18 6

Improved THRC_YEAST non-membrane 57438 5.3 3977 31 55 232 27 6

Default TKT1_YEAST non-membrane 73759 6.5 2011 41 47 253 30 7

Improved TKT1_YEAST non-membrane 73759 6.5 2710 54 51 313 35 5

Default TPIS_YEAST non-membrane 26778 5.6 54891 58 71 743 32 12

Improved TPIS_YEAST non-membrane 26778 5.6 39668 91 87 779 41 20

Default TRP_YEAST non-membrane 76578 6.0 6165 48 49 334 33 6

Improved TRP_YEAST non-membrane 76578 6.0 6760 55 57 413 37 5

Default TRX3_YEAST non-membrane 14422 9.2 3311 7 32 40 5 1

Improved TRX3_YEAST non-membrane 14422 9.2 4373 8 39 42 5 0

Default TRXB1_YEAST non-membrane 34216 5.6 4579 18 59 110 12 3

Improved TRXB1_YEAST non-membrane 34216 5.6 5453 18 46 124 9 0

Default TSA1_YEAST non-membrane 21576 4.8 28067 28 76 281 17 5

Improved TSA1_YEAST non-membrane 21576 4.8 18578 24 45 191 12 3

Default UGPA1_YEAST non-membrane 55953 7.1 9724 64 70 468 39 13

S-40

Improved UGPA1_YEAST non-membrane 55953 7.1 9589 72 77 514 47 14

Default URA8_YEAST non-membrane 64469 5.3 906 21 40 134 19 3

Improved URA8_YEAST non-membrane 64469 5.3 1668 31 48 157 24 0

Default VPS1_YEAST non-membrane 78687 8.1 3977 49 52 426 42 9

Improved VPS1_YEAST non-membrane 78687 8.1 9832 71 67 545 49 11

Default WTM1_YEAST non-membrane 48353 5.0 5126 30 58 169 20 2

Improved WTM1_YEAST non-membrane 48353 5.0 3650 24 39 164 13 3

Default YB085_YEAST non-membrane 9398 5.1 3553 9 95 63 9 1

Improved YB085_YEAST non-membrane 9398 5.1 1827 6 87 36 6 0

Default YBY9_YEAST non-membrane 57602 5.0 967 13 26 83 12 4

Improved YBY9_YEAST non-membrane 57602 5.0 1364 8 14 49 6 0

Default YCY0_YEAST non-membrane 20690 4.6 3748 18 74 119 14 2

Improved YCY0_YEAST non-membrane 20690 4.6 5115 16 61 88 12 3

Default YIM1_YEAST non-membrane 41611 7.9 1686 14 43 85 11 2

Improved YIM1_YEAST non-membrane 41611 7.9 1548 21 46 93 14 2

Default YMY9_YEAST non-membrane 33934 5.7 4536 28 57 148 18 1

Improved YMY9_YEAST non-membrane 33934 5.7 5901 28 67 175 19 3

Default YN034_YEAST non-membrane 10774 9.4 1443 7 64 27 5 0

Improved YN034_YEAST non-membrane 10774 9.4 3050 8 78 48 6 0

Default YNB0_YEAST non-membrane 27463 5.1 2710 9 49 65 9 1

Improved YNB0_YEAST non-membrane 27463 5.1 1585 11 39 53 7 1

Default YPR1_YEAST non-membrane 34733 6.7 2978 20 58 122 15 3

Improved YPR1_YEAST non-membrane 34733 6.7 2511 17 55 130 14 0

Default ZUO1_YEAST non-membrane 48990 8.7 17904 57 67 613 39 6

Improved ZUO1_YEAST non-membrane 48990 8.7 15749 59 52 401 28 3

Default YM71_YEAST unknown 29140 6.4 6207 18 71 125 13 1

Improved YM71_YEAST unknown 29140 6.4 5983 15 51 105 9 1

Default YM94_YEAST unknown 38191 6.2 3619 19 46 121 13 2

Improved YM94_YEAST unknown 38191 6.2 1896 17 55 80 13 1