1QUARTER - ISWEDISH EXCELLENCE IN CHROMATOGRAPHY

KANAK HPLC and UHPLC COLUMNS

NEWSLETTER2019QUARTER - I

ION PAIRED CHROMATOGRAPHY

ANALYSIS OF FOOD INGREDIENTS BYAN ANALYTICAL TECHNIQUE

SIMULTANEOUS DETERMINATION OFCALCIUM CHANNEL BLOCKERS

USING KANAK COLUMNS

SWEDISH EXCELLENCE IN CHROMATOGRAPHY

KANAK HPLC and UHPLC COLUMNS

NEWSLETTER2019QUARTER - I

ION PAIRED CHROMATOGRAPHY

ANALYSIS OF FOOD INGREDIENTS BYAN ANALYTICAL TECHNIQUE

SIMULTANEOUS DETERMINATION OFCALCIUM CHANNEL BLOCKERS

USING KANAK COLUMNS

2QUARTER - I

CONTACT DETAILS AND ORDER INFORMATIONFor company information, technical support, and order inquires:

Mr. Kshitij Shah

+91-9833772296 | info@nanghavi.com | www.nanghavi.com

M1 Kalpita Enclave,

Sahar Road,

Andheri (East),

Mumbai-400069.

IS A PART OF

• C18• C18 Plus• C8• C4• Phenyl Hexyl• Phenyl Hexyl Plus• PFP• Cyano

Wide range of chemistries to leverage all type of analytes.

• 2.1 mm• 3.0 mm• 4.6 mm• 10 mm*

Choose column IDs for your application; from MS to analytical to semi-preparative purposes.

8 PHASES

4 COLUMN IDs

• 250 mm• 150 mm• 100 mm• 50 mm

Choose the right length for your needs; resolution power to short analytical times.

4 COLUMN LENGTHS

• 2.6 µm• 3.5 µm• 5 µm• 10 µm*• 13 µm*

Particle sizes made for UHPLC and HPLC purposes.

5 PARTICLE SIZES

3QUARTER - I

4QUARTER - I

5QUARTER - I

Injection Volume (µl) Concentration (mM) Rf of Hygromycin B15 33 4.3110 50 4.277 71 4.225 100 4.134 125 4.133 167 4.13

6QUARTER - I

7QUARTER - I

SimultaneousDeterminationofCalciumchannelblockersusingKanakcolumns

Calciumchannelblockers(CCB)orCalciumantagonistsare group of prescribed cardiovascular medicationsused for treating high blood pressure and angina,arrhythmia and Raynaud`s phenomenon (a conditionin which arteries in the hands and feet arenarrowed)1,2. They prevent calcium from enteringcardiacandbloodvesselwalls3.

This CCBs can be determined by various techniqueslike mass-spectrometric4, thin-layer chromatographic5,radioreceptorassay6,gaschromatographic7and liquidchromatographic methods8. These methods focus onthe analysis of individual drugs. However, fortherapeutic treatment combined therapy is used9 andthus, development of analytical method capable ofsimultaneous determination is important10.Simultaneousdeterminationofthesedrugscanalsobeuseful in forensic toxicology to gain informationconcerning which drugs have been ingested prior todeath9.The exhaustive literature survey revealed thatmost of the earlier analytical methods suffer a lack ofspecificityandselectivity.SomeofthemethodslikeGCand HPLC include laborious liquid-liquid and solid-phase extraction procedures to give adequate sampleclean-up, thereby increasing the complexity and timerequired for analysis. Liquid chromatography–massspectrometryandliquidchromatography–tandemmassspectrometry (LC/MS/MS) are expensive and thusunavailableinmanylaboratories

SomeoftheCCBsweredeterminedsimultaneouslyonKanak columns in different mobile phases as shownbelow:

A

References1. WebMD.HeartDiseaseandCalciumChannelBlockerDrugs.2–4(2019).Availableat:https://www.webmd.com/heart-disease/guide/heart-disease-calcium-channel-blocker-drugs#1.2. O’Shaughnessy,D.K.M.HeartMattersDrugcabinet :CalciumchannelblockersHowdoCCBswork ?1–6(2019).Availableat:https://www.bhf.org.uk/informationsupport/heart-matters-magazine/medical/drug-cabinet/calcium-channel-blockers.3. Staff,M.C.Calciumchannelblockers.2–4(2019).Availableat:https://www.mayoclinic.org/diseases-conditions/high-blood-pressure/in-depth/calcium-channel-blockers/art-20047605?p=1.4. AnaB.Baranda,*†ClaudiaA.Mueller,†RosaM.Alonso,*RosaM.Jime´nez,andW.QuantitativeDeterminationoftheCalciumChannelFelodipine,andLacidipineinHumanPlasmaUsingLiquidChromatography-TandemMassSpectrometry.27,44–52(2005).

B

C

Figures: Chromatograms of simultaneouslydetermined CCBs on different Kanak columns andmobilephasecompositions.ConclusionThe CCBs are important therapeutic for treatment ofvarious cardiovascular diseases. These CCBs can begivenincombinationinsomecases.Thus,iftheseCCBsare determined simultaneously it can help to developeffective combination therapy for more faster results.Also, this can be helpful in forensics to determineinformation against different CCBs. The simultaneousdetermination of these drugs can be used in clinicalresearch to studydifferentdrug interactionsand theirpharmacokineticstudies.This simultaneous determination of CCBs tried ondifferent Kanak columns and mobile phases showsresults in less time (up to 16 minutes). Thus, Kanakcolumnscanbeconsidered todetermineandperformroutineanalysisoftheseCCBsindifferentsamplesandformulations.

5. SigridMennickent*,JonathanContreras,B.S.andM.deD.Highperformancethinlayerchromatographicdeterminationofnifedipineinhumanserumafterliquid-liquidextraction.35,411–415(2012).6. Gerbeau,C.&Barradas,J.ARadioreceptorAssayforDeterminationofNicardipineinHumanSerumUsingH(+)PN200-110asRadioligand.1522,(2006).7. Guellec,C.Le,Bun,H.,Giocanti,M.&Durand,A.DeterminationofNifedipineinPlasmabyaRapidCapillaryGasChromatographicMethod.6,20–23(1992).8. Wang,W.etal.FitoterapiaSimultaneousdeterminationofarctiinanditsmetabolitesinraturineandfecesbyHPLC.Fitoterapia86,6–12(2013).9. Kristoffersen,L.etal.Simultaneousdeterminationof6beta-blockers,3calcium-channelantagonists,4angiotensin-IIantagonistsand1antiarrhytmicdruginpost-mortemwholebloodbyautomatedsolidphaseextractionandliquidchromatographymassspectrometryMethoddevelopmentandrobustnesstestingbyexperimentaldesign.850,147–160(2007).10.Garcı,M.A.AcommonmethodforthedeterminationofseveralcalciumchannelblockersusinganHPLCsystemwithultravioletdetection.47,1245–1254(1998).

SimultaneousDeterminationofCalciumchannelblockersusingKanakcolumns

Calciumchannelblockers(CCB)orCalciumantagonistsare group of prescribed cardiovascular medicationsused for treating high blood pressure and angina,arrhythmia and Raynaud`s phenomenon (a conditionin which arteries in the hands and feet arenarrowed)1,2. They prevent calcium from enteringcardiacandbloodvesselwalls3.

This CCBs can be determined by various techniqueslike mass-spectrometric4, thin-layer chromatographic5,radioreceptorassay6,gaschromatographic7and liquidchromatographic methods8. These methods focus onthe analysis of individual drugs. However, fortherapeutic treatment combined therapy is used9 andthus, development of analytical method capable ofsimultaneous determination is important10.Simultaneousdeterminationofthesedrugscanalsobeuseful in forensic toxicology to gain informationconcerning which drugs have been ingested prior todeath9.The exhaustive literature survey revealed thatmost of the earlier analytical methods suffer a lack ofspecificityandselectivity.SomeofthemethodslikeGCand HPLC include laborious liquid-liquid and solid-phase extraction procedures to give adequate sampleclean-up, thereby increasing the complexity and timerequired for analysis. Liquid chromatography–massspectrometryandliquidchromatography–tandemmassspectrometry (LC/MS/MS) are expensive and thusunavailableinmanylaboratories

SomeoftheCCBsweredeterminedsimultaneouslyonKanak columns in different mobile phases as shownbelow:

A

References1. WebMD.HeartDiseaseandCalciumChannelBlockerDrugs.2–4(2019).Availableat:https://www.webmd.com/heart-disease/guide/heart-disease-calcium-channel-blocker-drugs#1.2. O’Shaughnessy,D.K.M.HeartMattersDrugcabinet :CalciumchannelblockersHowdoCCBswork ?1–6(2019).Availableat:https://www.bhf.org.uk/informationsupport/heart-matters-magazine/medical/drug-cabinet/calcium-channel-blockers.3. Staff,M.C.Calciumchannelblockers.2–4(2019).Availableat:https://www.mayoclinic.org/diseases-conditions/high-blood-pressure/in-depth/calcium-channel-blockers/art-20047605?p=1.4. AnaB.Baranda,*†ClaudiaA.Mueller,†RosaM.Alonso,*RosaM.Jime´nez,andW.QuantitativeDeterminationoftheCalciumChannelFelodipine,andLacidipineinHumanPlasmaUsingLiquidChromatography-TandemMassSpectrometry.27,44–52(2005).

B

C

Figures: Chromatograms of simultaneouslydetermined CCBs on different Kanak columns andmobilephasecompositions.ConclusionThe CCBs are important therapeutic for treatment ofvarious cardiovascular diseases. These CCBs can begivenincombinationinsomecases.Thus,iftheseCCBsare determined simultaneously it can help to developeffective combination therapy for more faster results.Also, this can be helpful in forensics to determineinformation against different CCBs. The simultaneousdetermination of these drugs can be used in clinicalresearch to studydifferentdrug interactionsand theirpharmacokineticstudies.This simultaneous determination of CCBs tried ondifferent Kanak columns and mobile phases showsresults in less time (up to 16 minutes). Thus, Kanakcolumnscanbeconsidered todetermineandperformroutineanalysisoftheseCCBsindifferentsamplesandformulations.

5. SigridMennickent*,JonathanContreras,B.S.andM.deD.Highperformancethinlayerchromatographicdeterminationofnifedipineinhumanserumafterliquid-liquidextraction.35,411–415(2012).6. Gerbeau,C.&Barradas,J.ARadioreceptorAssayforDeterminationofNicardipineinHumanSerumUsingH(+)PN200-110asRadioligand.1522,(2006).7. Guellec,C.Le,Bun,H.,Giocanti,M.&Durand,A.DeterminationofNifedipineinPlasmabyaRapidCapillaryGasChromatographicMethod.6,20–23(1992).8. Wang,W.etal.FitoterapiaSimultaneousdeterminationofarctiinanditsmetabolitesinraturineandfecesbyHPLC.Fitoterapia86,6–12(2013).9. Kristoffersen,L.etal.Simultaneousdeterminationof6beta-blockers,3calcium-channelantagonists,4angiotensin-IIantagonistsand1antiarrhytmicdruginpost-mortemwholebloodbyautomatedsolidphaseextractionandliquidchromatographymassspectrometryMethoddevelopmentandrobustnesstestingbyexperimentaldesign.850,147–160(2007).10.Garcı,M.A.AcommonmethodforthedeterminationofseveralcalciumchannelblockersusinganHPLCsystemwithultravioletdetection.47,1245–1254(1998).

8QUARTER - I

SimultaneousDeterminationofCalciumchannelblockersusingKanakcolumns

Calciumchannelblockers(CCB)orCalciumantagonistsare group of prescribed cardiovascular medicationsused for treating high blood pressure and angina,arrhythmia and Raynaud`s phenomenon (a conditionin which arteries in the hands and feet arenarrowed)1,2. They prevent calcium from enteringcardiacandbloodvesselwalls3.

This CCBs can be determined by various techniqueslike mass-spectrometric4, thin-layer chromatographic5,radioreceptorassay6,gaschromatographic7and liquidchromatographic methods8. These methods focus onthe analysis of individual drugs. However, fortherapeutic treatment combined therapy is used9 andthus, development of analytical method capable ofsimultaneous determination is important10.Simultaneousdeterminationofthesedrugscanalsobeuseful in forensic toxicology to gain informationconcerning which drugs have been ingested prior todeath9.The exhaustive literature survey revealed thatmost of the earlier analytical methods suffer a lack ofspecificityandselectivity.SomeofthemethodslikeGCand HPLC include laborious liquid-liquid and solid-phase extraction procedures to give adequate sampleclean-up, thereby increasing the complexity and timerequired for analysis. Liquid chromatography–massspectrometryandliquidchromatography–tandemmassspectrometry (LC/MS/MS) are expensive and thusunavailableinmanylaboratories

SomeoftheCCBsweredeterminedsimultaneouslyonKanak columns in different mobile phases as shownbelow:

A

References1. WebMD.HeartDiseaseandCalciumChannelBlockerDrugs.2–4(2019).Availableat:https://www.webmd.com/heart-disease/guide/heart-disease-calcium-channel-blocker-drugs#1.2. O’Shaughnessy,D.K.M.HeartMattersDrugcabinet :CalciumchannelblockersHowdoCCBswork ?1–6(2019).Availableat:https://www.bhf.org.uk/informationsupport/heart-matters-magazine/medical/drug-cabinet/calcium-channel-blockers.3. Staff,M.C.Calciumchannelblockers.2–4(2019).Availableat:https://www.mayoclinic.org/diseases-conditions/high-blood-pressure/in-depth/calcium-channel-blockers/art-20047605?p=1.4. AnaB.Baranda,*†ClaudiaA.Mueller,†RosaM.Alonso,*RosaM.Jime´nez,andW.QuantitativeDeterminationoftheCalciumChannelFelodipine,andLacidipineinHumanPlasmaUsingLiquidChromatography-TandemMassSpectrometry.27,44–52(2005).

B

C

Figures: Chromatograms of simultaneouslydetermined CCBs on different Kanak columns andmobilephasecompositions.ConclusionThe CCBs are important therapeutic for treatment ofvarious cardiovascular diseases. These CCBs can begivenincombinationinsomecases.Thus,iftheseCCBsare determined simultaneously it can help to developeffective combination therapy for more faster results.Also, this can be helpful in forensics to determineinformation against different CCBs. The simultaneousdetermination of these drugs can be used in clinicalresearch to studydifferentdrug interactionsand theirpharmacokineticstudies.This simultaneous determination of CCBs tried ondifferent Kanak columns and mobile phases showsresults in less time (up to 16 minutes). Thus, Kanakcolumnscanbeconsidered todetermineandperformroutineanalysisoftheseCCBsindifferentsamplesandformulations.

5. SigridMennickent*,JonathanContreras,B.S.andM.deD.Highperformancethinlayerchromatographicdeterminationofnifedipineinhumanserumafterliquid-liquidextraction.35,411–415(2012).6. Gerbeau,C.&Barradas,J.ARadioreceptorAssayforDeterminationofNicardipineinHumanSerumUsingH(+)PN200-110asRadioligand.1522,(2006).7. Guellec,C.Le,Bun,H.,Giocanti,M.&Durand,A.DeterminationofNifedipineinPlasmabyaRapidCapillaryGasChromatographicMethod.6,20–23(1992).8. Wang,W.etal.FitoterapiaSimultaneousdeterminationofarctiinanditsmetabolitesinraturineandfecesbyHPLC.Fitoterapia86,6–12(2013).9. Kristoffersen,L.etal.Simultaneousdeterminationof6beta-blockers,3calcium-channelantagonists,4angiotensin-IIantagonistsand1antiarrhytmicdruginpost-mortemwholebloodbyautomatedsolidphaseextractionandliquidchromatographymassspectrometryMethoddevelopmentandrobustnesstestingbyexperimentaldesign.850,147–160(2007).10.Garcı,M.A.AcommonmethodforthedeterminationofseveralcalciumchannelblockersusinganHPLCsystemwithultravioletdetection.47,1245–1254(1998).

9QUARTER - I

0 1 2 3 4 5 6 7 8 9Minutes

0 1 2 3 4Minutes

Antibiotics

CiprofloxacinHydrochloride Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.5mL/min

Temp: 30°CDetection: UV278nm

Eluent: 0.025MOrthophosphoricacid(pH3.0)/Acetonitrile=(87/13)

Performance:N/A

Cefazolin

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV270nm

Eluent: Water/Acetonitrile=(60/40)(pH8.0)

Performance:N/A

0 1 2 3 4 5 6

Minutes

0 1 2 3 4 5 6 7 8 9Minutes

PhospholipaseA2inhibitor

Xanthineoxidaseinhibitor

AnagrelideHydrochloride

Column: Kanak110Å5.0µmPhHex150x4.6mm

Flowrate: 1.2mL/min

Temp: 26°CDetection: UV254nm

Eluent: 0.05MPhosphatebuffer(pH2.5)/Acetonitrile=(25/75)

Performance:N/A

Febuxostat

Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV316nm

Eluent: 0.01MPhosphateBuffer(pH2.5)/Methanol=(20/80)

Performance:N/A

0 1 2 3 4 5 6 7 8 9Minutes

0 1 2 3 4Minutes

Antibiotics

CiprofloxacinHydrochloride Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.5mL/min

Temp: 30°CDetection: UV278nm

Eluent: 0.025MOrthophosphoricacid(pH3.0)/Acetonitrile=(87/13)

Performance:N/A

Cefazolin

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV270nm

Eluent: Water/Acetonitrile=(60/40)(pH8.0)

Performance:N/A

0 1 2 3 4 5 6 7 8 9Minutes

0 1 2 3 4Minutes

Antibiotics

CiprofloxacinHydrochloride Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.5mL/min

Temp: 30°CDetection: UV278nm

Eluent: 0.025MOrthophosphoricacid(pH3.0)/Acetonitrile=(87/13)

Performance:N/A

Cefazolin

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV270nm

Eluent: Water/Acetonitrile=(60/40)(pH8.0)

Performance:N/A

0 1 2 3 4 5 6

Minutes

0 1 2 3 4 5 6 7 8 9Minutes

PhospholipaseA2inhibitor

Xanthineoxidaseinhibitor

AnagrelideHydrochloride

Column: Kanak110Å5.0µmPhHex150x4.6mm

Flowrate: 1.2mL/min

Temp: 26°CDetection: UV254nm

Eluent: 0.05MPhosphatebuffer(pH2.5)/Acetonitrile=(25/75)

Performance:N/A

Febuxostat

Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV316nm

Eluent: 0.01MPhosphateBuffer(pH2.5)/Methanol=(20/80)

Performance:N/A

0 1 2 3 4 5 6

Minutes

0 1 2 3 4 5 6 7 8 9Minutes

PhospholipaseA2inhibitor

Xanthineoxidaseinhibitor

AnagrelideHydrochloride

Column: Kanak110Å5.0µmPhHex150x4.6mm

Flowrate: 1.2mL/min

Temp: 26°CDetection: UV254nm

Eluent: 0.05MPhosphatebuffer(pH2.5)/Acetonitrile=(25/75)

Performance:N/A

Febuxostat

Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV316nm

Eluent: 0.01MPhosphateBuffer(pH2.5)/Methanol=(20/80)

Performance:N/A

0 1 2 3 4 5 6 7 8 9Minutes

0 1 2 3 4Minutes

Antibiotics

CiprofloxacinHydrochloride Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.5mL/min

Temp: 30°CDetection: UV278nm

Eluent: 0.025MOrthophosphoricacid(pH3.0)/Acetonitrile=(87/13)

Performance:N/A

Cefazolin

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV270nm

Eluent: Water/Acetonitrile=(60/40)(pH8.0)

Performance:N/A

0 1 2 3 4 5 6

Minutes

0 1 2 3 4 5 6 7 8 9Minutes

PhospholipaseA2inhibitor

Xanthineoxidaseinhibitor

AnagrelideHydrochloride

Column: Kanak110Å5.0µmPhHex150x4.6mm

Flowrate: 1.2mL/min

Temp: 26°CDetection: UV254nm

Eluent: 0.05MPhosphatebuffer(pH2.5)/Acetonitrile=(25/75)

Performance:N/A

Febuxostat

Column: Kanak110Å5.0µmC18

250x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV316nm

Eluent: 0.01MPhosphateBuffer(pH2.5)/Methanol=(20/80)

Performance:N/A

10QUARTER - I

0 1 2 3 4 5 6 7 8 9Minutes

Hormone

Ethinylestradiol Column: Kanak110Å5.0µmC18

150x4.6mmFlowrate: 1.5mL/minTemp: AmbientDetection: UV215nmEluent: Acetonitrile:Methanol:Water(7:3:9)

Ceftriaxone and Sulbactam

1. Ceftriaxone 2. Sulbactam

Column: Kanak 110 Å 5.0 µm C18 150 x 4.6 mm

Flow rate: 1.0 mL/min Temp: 25 °C Detection: UV 215 nm Eluent: 0.05 M Phosphate buffer (pH 3.5)/

Acetonitrile =(35/65)

Performance: N/A

1

2

0 1 2 3 4 5Minutes

5 6 7 8 9 10 11 12 13 14

Minutes

5 6 7 8 9Minutes

Antimalarialagent

Artemether

Column: Kanak110Å5.0µmC18250x4.0mm

Flowrate: 1.5mL/min

Temp: AmbientTemperatureDetection: UV216nm

Eluent: Acetonitrile/Water=(62/38)

Performance:N/A

Lumefantrine

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/min

Temp: 27°CDetection: UV210nm

Eluent: 0.01MPhosphatebuffer(pH3.0)/Acetonitrile=(25/75)

Performance:N/A

5 6 7 8 9 10 11 12 13 14

Minutes

5 6 7 8 9Minutes

Antimalarialagent

Artemether

Column: Kanak110Å5.0µmC18250x4.0mm

Flowrate: 1.5mL/min

Temp: AmbientTemperatureDetection: UV216nm

Eluent: Acetonitrile/Water=(62/38)

Performance:N/A

Lumefantrine

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/min

Temp: 27°CDetection: UV210nm

Eluent: 0.01MPhosphatebuffer(pH3.0)/Acetonitrile=(25/75)

Performance:N/A

Ceftriaxone and Sulbactam

1. Ceftriaxone 2. Sulbactam

Column: Kanak 110 Å 5.0 µm C18 150 x 4.6 mm

Flow rate: 1.0 mL/min Temp: 25 °C Detection: UV 215 nm Eluent: 0.05 M Phosphate buffer (pH 3.5)/

Acetonitrile =(35/65)

Performance: N/A

1

2

0 1 2 3 4 5Minutes

0 1 2 3 4 5 6 7 8 9Minutes

Hormone

Ethinylestradiol Column: Kanak110Å5.0µmC18

150x4.6mmFlowrate: 1.5mL/minTemp: AmbientDetection: UV215nmEluent: Acetonitrile:Methanol:Water(7:3:9)

5 6 7 8 9 10 11 12 13 14

Minutes

5 6 7 8 9Minutes

Antimalarialagent

Artemether

Column: Kanak110Å5.0µmC18250x4.0mm

Flowrate: 1.5mL/min

Temp: AmbientTemperatureDetection: UV216nm

Eluent: Acetonitrile/Water=(62/38)

Performance:N/A

Lumefantrine

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/min

Temp: 27°CDetection: UV210nm

Eluent: 0.01MPhosphatebuffer(pH3.0)/Acetonitrile=(25/75)

Performance:N/A

5 6 7 8 9 10 11 12 13 14

Minutes

5 6 7 8 9Minutes

Antimalarialagent

Artemether

Column: Kanak110Å5.0µmC18250x4.0mm

Flowrate: 1.5mL/min

Temp: AmbientTemperatureDetection: UV216nm

Eluent: Acetonitrile/Water=(62/38)

Performance:N/A

Lumefantrine

Column: Kanak110Å5.0µmC18250x4.6mm

Flowrate: 1.0mL/min

Temp: 27°CDetection: UV210nm

Eluent: 0.01MPhosphatebuffer(pH3.0)/Acetonitrile=(25/75)

Performance:N/A

11QUARTER - I

1

2

0 1 2 3 4 5 6 7 8 910 1112 1314 15 16 17 18Minutes

0 1 2 3 4 5 6 7 8 9

Minutes

Painkiller

H2(histamine-2)blockers

ParacetamolandIbuprofen

1. Paracetamol2. Ibuprofen

Column: Kanak110Å5.0µmC8250x4.6mm

Flowrate: 1.5mL/min

Temp: 25°CDetection: UV220nm

Eluent: 0.058MPhosphatebuffer(pH7.0)/Acetonitrile=(75/25)

Performance:N/A

Ranitidine

Column: Kanak110Å3.5µmC18100x4.6mm

Flowrate: 1.5mL/minTemp: 35°C

Detection: UV230nm

Eluent: SolutionA:0.05MPhosphatebuffer(pH7.1):Acetonitrile(95:5)SolutionB:0.05MPhosphatebuffer(pH7.1):Acetonitrile(78:22)

Gradient: 100-0%Ain0to10min0%Aat10to15min0-100%Ain15to16min

100%Ain16to20min

Performance:N/A

1

2

0 1 2 3 4 5 6 7 8 910 1112 1314 15 16 17 18Minutes

0 1 2 3 4 5 6 7 8 9

Minutes

Painkiller

H2(histamine-2)blockers

ParacetamolandIbuprofen

1. Paracetamol2. Ibuprofen

Column: Kanak110Å5.0µmC8250x4.6mm

Flowrate: 1.5mL/min

Temp: 25°CDetection: UV220nm

Eluent: 0.058MPhosphatebuffer(pH7.0)/Acetonitrile=(75/25)

Performance:N/A

Ranitidine

Column: Kanak110Å3.5µmC18100x4.6mm

Flowrate: 1.5mL/minTemp: 35°C

Detection: UV230nm

Eluent: SolutionA:0.05MPhosphatebuffer(pH7.1):Acetonitrile(95:5)SolutionB:0.05MPhosphatebuffer(pH7.1):Acetonitrile(78:22)

Gradient: 100-0%Ain0to10min0%Aat10to15min0-100%Ain15to16min

100%Ain16to20min

Performance:N/A

0 1 2 3 4Minutes

8 9 10 11 12 13 14 15 16 17 18

Minutes

BacteriostaticandLocalanesthetic

Vitamins

Fatsolublevitamins

BenzylAlcohol

Column: Kanak110Å5.0µmC18150x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV254nm

Eluent: Acetonitrile/Methanol=(60/40)

Performance:N/A

Cholecalciferol

Column: Kanak110Å5.0µmC18150x4.6mm

Flowrate: 1.0mL/min

Temp: 25°CDetection: UV254nm

Eluent: SolventA-60%AcetonitrileSolventB-40%Methanol

Gradient: 5%Aat0min

40%Aat15min

5%Aat16min

5%Aat30minutes

Performance:N/A

1

2

0 1 2 3 4 5 6 7 8 910 1112 1314 15 16 17 18Minutes

0 1 2 3 4 5 6 7 8 9

Minutes

Painkiller

H2(histamine-2)blockers

ParacetamolandIbuprofen

1. Paracetamol2. Ibuprofen

Column: Kanak110Å5.0µmC8250x4.6mm

Flowrate: 1.5mL/min

Temp: 25°CDetection: UV220nm

Eluent: 0.058MPhosphatebuffer(pH7.0)/Acetonitrile=(75/25)

Performance:N/A

Ranitidine

Column: Kanak110Å3.5µmC18100x4.6mm

Flowrate: 1.5mL/minTemp: 35°C

Detection: UV230nm

Eluent: SolutionA:0.05MPhosphatebuffer(pH7.1):Acetonitrile(95:5)SolutionB:0.05MPhosphatebuffer(pH7.1):Acetonitrile(78:22)

Gradient: 100-0%Ain0to10min0%Aat10to15min0-100%Ain15to16min

100%Ain16to20min

Performance:N/A

1

2

0 1 2 3 4 5 6 7 8 910 1112 1314 15 16 17 18Minutes

0 1 2 3 4 5 6 7 8 9

Minutes

Painkiller

H2(histamine-2)blockers

ParacetamolandIbuprofen

1. Paracetamol2. Ibuprofen

Column: Kanak110Å5.0µmC8250x4.6mm

Flowrate: 1.5mL/min

Temp: 25°CDetection: UV220nm

Eluent: 0.058MPhosphatebuffer(pH7.0)/Acetonitrile=(75/25)

Performance:N/A

Ranitidine

Column: Kanak110Å3.5µmC18100x4.6mm

Flowrate: 1.5mL/minTemp: 35°C

Detection: UV230nm

Eluent: SolutionA:0.05MPhosphatebuffer(pH7.1):Acetonitrile(95:5)SolutionB:0.05MPhosphatebuffer(pH7.1):Acetonitrile(78:22)

Gradient: 100-0%Ain0to10min0%Aat10to15min0-100%Ain15to16min

100%Ain16to20min

Performance:N/A

0 1 2 3 4Minutes

8 9 10 11 12 13 14 15 16 17 18

Minutes

BacteriostaticandLocalanesthetic

Vitamins

Fatsolublevitamins

BenzylAlcohol

Column: Kanak110Å5.0µmC18150x4.6mm

Flowrate: 1.0mL/minTemp: 25°C

Detection: UV254nm

Eluent: Acetonitrile/Methanol=(60/40)

Performance:N/A

Cholecalciferol

Column: Kanak110Å5.0µmC18150x4.6mm

Flowrate: 1.0mL/min

Temp: 25°CDetection: UV254nm

Eluent: SolventA-60%AcetonitrileSolventB-40%Methanol

Gradient: 5%Aat0min

40%Aat15min

5%Aat16min

5%Aat30minutes

Performance:N/A

12QUARTER - I

CONTACT DETAILS AND ORDER INFORMATIONFor company information, technical support, and order inquires:

Mr. Kshitij Shah

+91-9833772296 | info@nanghavi.com | www.nanghavi.com

M1 Kalpita Enclave,

Sahar Road,

Andheri (East),

Mumbai-400069.

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