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Figure S1. pαH vector map information.
Electronic Supplementary Material (ESI) for Biomaterials Science.This journal is © The Royal Society of Chemistry 2018
Figure S2. Phototoxicity of Fab-IR700 in co-culture of 3T3-GFP and 3T3-MDR1 cells.
(A) mixed 3T3-GFP and 3T3-MDR1 cells were stained with PI after Fab-IR700
mediated PDT; (B) mixed cells were treated with PBS (control). Scale bar for 4X,
1000 μm. Scale bar for 20X, 100 μm.
A
B
PI 3T3-GFP Merged
Merged
with DIC
4×Objective Lenses
20×Objective Lenses
4×Objective Lenses
20×Objective Lenses
PBS
Free IR700
Fab-IR700
100
80
60
40
20
0
101 102 103 104 105 106100 107
A B
0%
20%
40%
60%
80%
100%
120%
0.5 5 50 500
IR700 concentration (nM)
Cell V
iab
ilit
y
IR700
PBS
Free IR700
Fab-IR700
100
80
60
40
20
0
101 102 103 104 105 106100 107
IR700
3T3
3T3-MDR1
C
Calcein
AM
Merged
PI
Figure S3. (A) Flow cytometry analysis of Pgp negative 3T3 (up) or Pgp-expressing 3T3-
MDR1 (down) cells after treatment with free IR700 or Fab-IR700 at the same concentration
of IR700 (120 nM) for 4 h. (B) Dose-dependent phototoxicity of free IR700 in 3T3-MDR1
cells. Data are means ± SD (n = 3). (C) Live/Dead staining of 3T3-MDR1 cells after
treatment with Free IR700 (500 nM) followed by light irradiation. Scale bar, 1000 μm.
No
rma
lize
d T
o M
od
eN
orm
ali
ze
d T
o M
od
e
Calcein AM PI Merged
Pab-IR700
Fab-IR700
Figure S4. Phototoxicity of the Fab-IR700 conjugates in KB-3-1 tumor spheroids.
PI staining in KB-3-1 spheroids after Pab-IR700 or Fab-IR700 mediated PDT.
Spheroids were incubated with Pab-IR700 or Fab-IR700 for 24 hours followed by
light irradiation. Scale bar, 1000 µm.
1 h
2 h
24 h
Fig. S5. Ventral view of IR700 distribution
in a mouse xenograft model of
chemoresistant tumors, give a view of liver
and bladder accumulation for IR700.
Figure S6. Confocal images of intracellular distribution of Fab-IR700 and Pab-
IR700 in KB-8-5-11 tumors 24 h post injection. The scale bar is 20 μm.
DAPI CD31 IR700 Merged
Pab
-IR700
Fab-IR700
20 μm
100 μm
DAPI CD31 IR700 MergedPab
-IR700
Fab-IR700
Figure S7. Confocal images of intratumoral distribution of Fab-IR700 and Pab-
IR700 in KB-8-5-11 tumors 24 h post injection at a large scale view. Scale bar is
100 μm.
Fig. S8. Body weight of mice after targeted PDT.
Data are means ± SD, n=6.
0 5 10 15 20 2510
15
20
25
30
Days post xenografting
Bo
dy
we
igh
t(g
)
PBS
Fab-IR700
Pab-IR700
