Upload
usama-imtiaz
View
216
Download
0
Embed Size (px)
Citation preview
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
1/23
TAS2012 Proceeding Presentation
Nanobiotechnology Fusion System2013-01-08
5312FG02-2 Usama ImtiazDepartment of Integrative Bioscience and Biomedical
EngineeringWB Group, Takanishi Laboratory
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
2/23
Contents
1. MANIPULATING SINGLE PARTICLES USINGSTANDING SURFACE ACOUSTIC WAVES
2. 3D OMNIDIECTIONAL CONTOLLABLE ELASTIC IPMC
TWEEZER WITH SELF-SENSING AND ADJUSTABLECLAMPING FORCE ABILITIES FOR BIOMEDICAL
APPLICATIONS
2
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
3/23
Paper Introduction
MANIPULATING SINGLE PARTICLES USING
STANDING SURFACE ACOUSTIC WAVES
Xiaoyun Ding, Sz-Chin Steven Lin, Sixing Li, Lin Wang, and
Tony Jun Huang
The Pennsylvania State University, USA
3
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
4/23
Introduction
Manipulation of Micro/nano Objects e.g cells, bacteria, viruses, DNA etc.
Existing Manipulators and drawbacks:
Optical Tweezers High power laser, Complex, Expensive, Large
Magnetic Tweezers
Targets must be pre-labeled with Magnetic Material
Electrophoresis based Tweezers
Dependent on polarizability of particles,. Can adverselyaffect cell physiology
4
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
5/23
Acoustic-based particle manipulation
Advantages: Non-invasive
Work for a wider range of microparticles regardlessof their optical/electrical/magnetic properties.
Significance of current work:
Focusing, Separation, Sorting and Patterning havebeen realized by acoutic methods before.
Authors have fabricated acoustic tweezers capableto precisely control single microparticles in two-dimensions
5
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
6/23
Design Overview
A PDMS channel is bonded to a Lithium Niobatepeizoelectric substrate.
Two pairs of chirped Interdigital transducers
IDTs are also present on the substrate.
6
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
7/23
Working
IDT pairs are independently fed RF signals togenerate surface acoustic waves (SAWs), whichinterfere to form standing waves.
Acoustic radiation force drives particles tonodes or anti-nodes depending on their elasticproperties, density, compressibility etc.
Position of nodes depend on the acousticwavelength xn=n/2
7
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
8/23
Particle/cell manipulation in 2D
8
9
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
9/23
Particle Manipulation
9
10
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
10/23
Physiological Effect on Sample
HeLa cells were exposed to high power standing SAWfields for 6s, 1 min, 10 min.
The results indicate that there was no signifantphysiological damage induced to the cell
10
11
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
11/23
Multiple particles
Simultaneouslylarge number ofparticles can bemanipulated.
Clusters of
particles can bearranged on asingle node ormultiple nodes.
11
12
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
12/23
Conclusion
Small, Portable, inexpensive particle-manipulation system.
Can also be used for sorting (if combined with
flow-based microfluidics) Can dexterously manipulate single particles
Can handle multiple particles simultaneously,
grouped or individually. Have no adverse physiological effect on
samples
12
13
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
13/23
Second Paper Introduction
3D OMNIDIECTIONAL CONTOLLABLE ELASTIC
IPMC TWEEZER WITH SELF-SENSING AND
ADJUSTABLE CLAMPING FORCE ABILITIES FORBIOMEDICAL APPLICATIONS
Guo-Hua Feng* and Jen-Wei Tsai
National Chung Cheng University, Chiayi, Taiwan
13
14
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
14/23
Introduction
3D omnidirectionalaccessible IPMC elastictweezers
Can move multi-directionallyAdjustable clasping force
Self-sensing force by
feedback current
14
15
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
15/23
Issues
Flexible Ionic polymer metal composites (IPMCs)have great potential as soft robotic actuators.
Conventional Preparation:
Ionic polymer formation
Compositing
Surface electroding
Usually prepared in large scale and simple shaped
IPMC Transducers: Planar IPMC structure withNafion sandwiched between plated metalelectrodes. (PolyTetraFluroEthylene and
Perfluorinated vinyl ether sulfonate)
15
16
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
16/23
Fabrication
16
17
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
17/23
Electrode plating
17
18
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
18/23
Experimental setup
IPMC Tweezer can be inserted in the fixture Force balance
18
19
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
19/23
Characterization Front-back movement
19
20
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
20/23
Characterization Sideways
20
21
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
21/23
3D IMPC tweezer capabilities
21
22
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
22/23
Tweezer force and current relationship
Current flowing throughthe transducer can becalibrated to gauge thetweezers dynamic force.
Can determine iftouching/holding theobject and tosense/adjust clampingforce
22
23
7/30/2019 2012-01-08 Usama Imtiaz 5312FG02-2 Nanobiotechnology Presentation
23/23
Conclusion
3D omnidirectional IPMC tweezer isdemonstrated
IPMC Transducer conmprises:
Motion element Clamping head
Can move multidirectionally
Can provide adjustable clasping force Has many applications
23