BIOPOD seriesThe Protein Machine on the Bench

What makes the difference ?What makes the difference ?

Expandable with No Compromise Do it all Faster and Better

Access Incredible Benefits with the BIOPODThe protein machine on the bench

Proven Scalable Results

Scalability to larger conventional pilot fermentor has been proved with similar

sensors, parameter control ability and performance.

The bioPOD multi fermentor platform can be used to scale-up or scale down

existing fermentation models (process optimization) with biological recipes built

in the system, customised or other define biological model implemented into the

software platform.

The Advantages

Compare to traditional scale-up or scale-down operation in fermentation, the biopod platform is offering significant

advantages:

- The fermentation is driven by a remote biological model

- Insight and Improvement of the robustness of a process ( for PAT and Quality by Design initiatives)

- Independent fermentor to study at same time different biological strategies and enhance the production of the

targeted protein

- New design of recipes is validated before use in routine conditions by the end user

- Culture are started any time without an operator when fermentor are used in an remote mode with a built in recipe

Introduction

The challenge for Process Development, Media optimization, Process optimization or clone screening lies in the limited

performance of available equipment using traditional scale–up or scale-down methods and equipments at bench scale. Culture

model as shake flask, well plates or disposable multi reactors do not provide so far the automated biological culture models or

recipes of culture to get faster results. The bioPOD is at bench scale, representative of a true scale up method and recipes able to

be transferred at a manufacturing stage with biological control on the process.

Reduce and Optimize R&D and Process Development Efforts

The bioPOD is a multi fermentation platform (POD) which enable multi-factorial fermentation runs based on

built-in biological recipes of culture for aerobic or anaerobic fermentation. The existing built-in recipes are

capable of performing fully automated batch, fed-batch or chemostat processes with pre-defined standard

culture recipes but also able to be customized due to the modularity of the software platform.

Compact Modular Design

Available in single, twin, triple and up to 64 units, the bioPOD design is based on the use of an ergonomic,

modular construction housing the local controller as well as additional gassing, pump and various modules.

This result in considerable savings in valuable benchtop space.

The powerful local controller built in a tower arrangement or separate as modules (shelf stackable) offers a

comprehensive range of control and display facilities, like as up to 9 automated biological recipes and for

classic remote control cascadable PID control loops, x-stage cascade control for pO2, alarm monitoring, recipe

control and password protection for critical system parameters

In addition there are software modules like specific biological

recipes customized and customizable to the targeted protein to

be expessed:

In combination with its software capabilities, the bioPOD is a

concept fully designed to change the way fermentor

performance must be achieved with less running time and less

complexity.

Unique Benefits

Modular and Scalable from 100 ml up to 10 L Working Volume

Biological Recipes Monitoring the Fermentation Processes

Optical and capacitance sensors Coupled for enhanced fermentation control

Fully Remote Process control customizable to Specific Fermentation Needs

Ready to Use Induction models built in the Software Package

Easy Connection to Sensors

Simple Modular Design on the Bench or Wall Mounted

Dual Peltier module with Built-In OD Sensors

Advanced Software

The software platform is designed to support biological models based on physical parameters

to control automatically the growth of cells.

Basically designed for protein production with E. coli the bioPOD can be also used with any

bacteria strength and yeast such as Pichia Pastoris or Saccharomyces.

This innovative and unique method of supervision lead to a concept where cells are managing

the process instead of using only the physical parameters as the way to manage and control a

fermentation run.

Data storage of graphs and recipes with retrieval functions for an easy access to existing data.

F200 unit with 2 x 100 ml bioreactors

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Vessel Biopod serie F200 F1 F3 F5 F10

Min Working Volume (liter) 0,03 0,2 0,4 1 3,5

Max Working Volume (liter) 0,1 1 2,9 6 11

Material Borosilicated Glass, 316 L S/S

Control unit Control Panel Color Touch Panel

Calibration Local Easy Interface

Communication Port RS 485

Program Storage Full Embedded Software

Log Data Storage Remote Controlling Software.

Cabinet material ABS

Dimension (inch) 14.6'' x 28.8" x 9.1" (W x H x D)

Aeration Inlet Gas Flowmeter TMFC for Air/O2 up to 2.5 VVM

Sparger Sintered

Temperature Control System Chiller, Heating Belt

Range 4 or 14 C up to 65 C

Resolution 0,1 C

Probe Platinum RTD Probe (PT100)

Control mode Manual, Auto (PID), Remote

Agitation Drive Removable Top Brushless Motor

Speed Range 30 – 1200 RPM

Resolution 1 RPM

Impeller 1,2 or 3 Rushton Impellers

Control mode Manual, Auto

F200 system Gas lift

pH Range 2 – 12 pH

Resolution 0,01 Unit

Electrode Gel Filled, Autoclavable

Control Mode On/Off, Adjustable PID 1Way, 2 Ways, Manual, Auto, Remote

DO Range 0 – 470 % (100% for air)

Resolution 0,1 %

Probe Polarographic or optical, Autoclavable

Control Mode Adjustable PID, Cascade, Manual, Auto, Remote

OD Range 0 – 4 AU

Resolution

Probe Optical, Autoclavable, USP Class VI Materials.

Control Mode Adjustable via Recipe Control

F200 system Integrated external sensor

Capacitance Range 1g/L – 200g/L DCW for Yeast, Bacteria and Fungi

5g/L min with Highly Aerated Media

0 - 700pF/m Max Permittivity; 0.5 – 100mS/cm Conductance

Resolution 0.02g/L DCW for Yeast

Probe Capacitive, Autoclavable, USP Class VI Materials.

Control Mode Adjustable via Recipe Control

Foam Probe 316 L S/S Height Adjustable

Control Mode ON/OFF Control

Peristaltic pumps Pump number 6 Built-in Pumps, 2 External pumps

Motor Type Pulse Driven Motor for

Flow Rate minimum 0.15μL each 46h up to nominal flow rate.

Speed 47 RPM, 100RPM (other on request)

Nominal Flow rate 3.2ml/min (built in), 75ml/min (external). (other on request)

Resolution 0.15μL

Control mode On/Off, Manual, Auto, Remote

Pump(s) can be Assigned for Acid, Base, Inoculum, Inducer, Anti-Foam and Substrates

Off Gas Device type Glass or 316 L S/S Condenser

Utility requirement Power Supply 110V or 230V; 50/60 Hz with Safety Cut off Switch

Water No Water supply Connection with chiller

Gas 1.2 bar Max. Medical Grade, dry, oil free and filtered

Specifications

BIOPODThe protein machine on the bench

Europe FOGALE nanotechNîmes, FrancePhone: +33 (0)4 66 62 05 55infobiotech@fogale.com

USA / Asia FOGALE biotech, Inc.Cambridge, MA 02140Phone: (818) 206 8525infobiotech@fogale.com