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ACE Forum - Alliance for Cooperation in Engineering

Porto, FEUP, May 5, 2014

Pedro L. Granja

INEB - Instituto de Engenharia Biomédica

Universidade do Porto, Porto, Portugal

pgranja@ineb.up.pt

INEB INSTITUTO DE ENGENHARIA BIOMÉDICA

www.ineb.up.pt

40 prototypes

1989founded

3spin-offs

>6products

Biomaterials

Tissue regeneration

Nanomedicine

Bioimaging

Biomedical signals

Nephrology

Geoepidemiology

people

>200PhDs

60

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Research Groups

• Biocomposites - FJ Monteiro

• Biocarrier - Biomaterials for Multistage Drug and Cell Delivery -

PL Granja

• NanoBiomaterials for Targeted Therapies - AP Pêgo

• Nephrology and Infecciology R&D - M Pestana

• Microenvironments for Newtherapies - MA Barbosa

• Monitoring and Simulation of Perinatal Asphyxia -

D Ayres de Campos

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Main clinical areas of application

• Orthopedics

• Neurosurgery

• Cardiovascular surgery

• Nephrology

• Oncology

Composite cryogels for bone tissue regeneration

Type I collagen/nanoHA sponges for bone tissue enginneering 3D hBMSC cultures

Teixeira et al,

J Biomed Mater Res A (2010)

In vivo experiments in rats.

De novo bone being formed with adequate

vasculature and inflammation zone being

progressively reduced

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SiO2+ nanoHA

coatings on zirconia

Microstructured anisotropic surfaces for

guided tissue growth on dental implants

StampingStamping

Sintering 500ºC

Pelaez-Vargas et al, Dental Materials (2011)

Multi-functional and biospecific hydrogels

for cell delivery

• Multi-functional hydrogels for the entrapment and delivery of (stem) cells

• Binding of specific cell-interactive oligopeptides to mimic essential features of the ECM: cell adhesion, proteolytic degradation and guided stem cells differentiation

Cell adhesion on

RGD-alginate

Evangelista, Biomaterials (2007)

Bidarra, Biomacromolecules (2010)

Cell adhesion and migration on

PVGLIG-RGD-alginate

Fonseca, Acta Biomater (2011)

Cell differentiation on

OGP-PVGLIG-RGD-alginate

Maia (Submitted 2013)

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DNA /βIII / GFAP

Scaffolds and Neural stem

cells in pre-endothelialized

scaffolds

Neural stem cell-endothelial cell

cross-talk

Drug-loaded aligned nanofibers for

axonal growth orientation and

enhancement

Matrix-driven stem-cell regenerative

therapies for the spinal cord injury

Chitosan microspheres for specific

H. Pylori-binding

gold

Non-fouling surface background

Average size: d = 170 ±15 µm

Chitosan (Squid pen; DA 15%)

H. pylori adhesion to

chitosan microspheres

SEM Confocal Microscopy

Chitosan microspheres

Parreira et al, J Biomed Mater Res (2011)

Glycosylated compounds

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Inflammation

Endogenous

regeneration

Cancer

Cell recruitment (e.g. MSC)

Cell invasion (regeneration and cancer)

IVD regeneration

In collaboration with

A cell-line model-system for adult heart Sca1+ progenitor cells(Manuscript submitted)

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Improved lef ventricle performance In vivo engraftment into the infarcted heart

DAPI Y-Chrm. Cnx43 DAPI Y-Chrm. α-Actinin

Stem cell therapies for cardiac regeneration

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Nanomedicine

Regenerative

Medicine

Biomimetic

Strategies

Drug Delivery and

Diagnostic SystemsNanoimaging

Cancer diagnosisEthical issues, epidemiological studies

Delivery of therapeutic genes to the peripheral

nervous system in a targeted fashion

Non-toxic, targeted, simple and

efficient nanoparticle system to

specifically transfect PNS neurons

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Force Spectroscopy as a tool for

screening targeting moieties

Oliveira et al, J Control Release (2010)

Oliveira et al, Small 2011

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Retinal blood vessels are windows to the cardiovascular system. Objective evaluation of arteriolar narrowing can give an indication of risk of stroke, cerebral atrophy, or myocardial infarct. This system measures automatically the arteriolar-to-venular diameter ratio (AVR), which can be used as an objective risk indicator.

TRC-NW8 High Resolution Non-Myd System, http://www.topconmedical.com/products/trcnw8highresolutionnonmydsystem.htm

http://www.corporatewellnessprogram.net/retinal-imaging/

Retinal Imaging System OverviewInput Image

AVR

Artery and vein

diametersArtery–vein classification

Ring measuring region

Augmenting diagnostic skillsMeasuring arteriolar-to-venular diameter ratio (AVR) in retinal images

Spatial epidemiological studies on orthopedic diseases

• The Bone Decade in Portugal• Spatio-temporal trends in the incidence of hip fractures

• Risk of falls – actions for health education in the elderly

• Clinical Indicators for quality improvement in hospitalizations by hip

fracture

• Longitudinal study of hip fracture patients (cohort study)

• The burden of Hip and Knee Arthroplasties• Epidemiology of Total Hip and Knee Replacements

• National and International Studies

• Analyses of Failures of Explanted Hip Prostheses

• Collaboration with Newtherapies Group and 3 hospitals in Porto

Region

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Kurtz et al, Int Orthop (2011)

Ribeiro et al, Int J Cardiol (2011)

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Technology Transfer>6 Products in the market

• Actifuse® – Bone filler material made of modified calcium phosphate, Baxter (USA)

• NanoXIM® - NanoHydroxyapatite produced using the innovativeproprietary technology NETmix®, Fluidinova (Maia)

• WalkinSense® - Medical device for diabetic foot monitoring, Tomorrow Options (Porto)

• BoneLike® - Bone replacement materials made of modified synthetic hydroxyapatite, MedMat Innovation (Maia)

• Intrapartum acute care simulator - METi - Medical Education Technologies (USA)

• Omniview-SisPorto® - Fetal monitoring central system with SisPorto automated analysis, Speculum (Lisbon)

SiSPorto Omniview®Automatic analysis of fetal cardiotochogramme

• Commercialized by Speculum, Lisbon

• Installed in:

• Hospital de S. João

• Hospital de Leiria

• Hospital da Feira

• Hospital de Setúbal

• Maternity Alfredo da Costa (soon)

• Validated in an international study with the participation of centres

from: Germany, Australia, UK, Belgium, Spain, Denmark, Switzerland,

Finland, Sweden, Portugal

• Ongoing intrapartum validation study (500-600 cases)

• SisPorto already provides foetal electrocardiography data (system

STAN)

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The Biomedical Simulation CentreUniversity of Porto, Faculty of Medicine

Licensing and development agreements were signed with the American company METi in 2007.

Full-scale model-driven medical simulators save lives in anesthesia.

Human errors in health care cause mortality and morbidity.

Consortia and networks

• Consortia

• Networks

The European Society for Biomaterials

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University of Porto

I3S

IPOCH PortoCH SJoãoFFUPFEUPFCUPFMDUPICBASFMUPINEBIBMCIPATIMUP

Host Interaction and Response

CancerNeurobiology and Neurologic

Disorders

I3S – Instituto de Investigação e Inovação em Saúde

(Institute for Research and Innovation in Health)

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Participation in Horizon 2020

• Focus on disease: Societal challenges

• Dialysis

• Cancer diagnosis and treatment

• Inflammation in Tissue regeneration

• Epidemiology – ageing, risk of falls

• Focus on technologies: Industrial leadership

• Biomaterials – skin, nerve and bone regeneration

• Additive manufacturing (bioprinting) – skin regeneration

• Microfluidics (organ-on-a-chip) – stomach

• Nanomedicine (gene and drug delivery) – stomach cancer, diabetes, HIV, nerve regeneration

Topics applied to in the 2014 call Health, demographic change and wellbeing

• Two-stage

• Personalising Health and Care (PHC) 1 - 2014: Understanding health, ageing and disease: determinants, risk factors and pathways - Falls and their prevention (Fátima Pina)

• PHC10 - 2014: Development of new diagnostic tools and technologies: in vitro devices, assays and platforms - Diagnosis kit for cancer (Meriem Lamghari)

• PHC 23 - 2014: Developing and comparing new models for safe and efficient, prevention oriented, health and care systems - Care models for end of life patients (Maria Oliveira/Fátima Pina, coordinated by INEB)

• Single stage

• PHC 15 – 2014/15: Clinical research on regenerative medicine - Biomaterials for regenerative medicine (Ana Paula Pêgo/Cristina Martins)

• PHC 26 - 2014: Self-management of health and disease: mHealth - Mobile platform for dialysis patients care (Manuel Pestana, coordinated by INEB)

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Topics applied to in the 2014 call Nanotechnologies, Advanced Materials and Production

• Nanotechnologies, Advanced Materials and Production (NMP)-08-2014:

“Scale-up of nanopharmaceuticals production” - Production of novel GMP

nanoparticles for pharmaceutical applications (Bruno Sarmento)

Topics of interest in the 2015 call

• Health, demographic change and wellbeing

• PHC 3: Understanding common mechanisms of diseases and their relevance in co-morbidities

• PHC 4: Health promotion and disease prevention: improved inter-sector co-operation for environment and health based interventions

• PHC 14: New therapies for rare diseases

• PHC16: Tools and technologies for advanced therapies

• PHC 24: Piloting personalised medicine in health and care systems

• PHC 27: Self-management of health and disease and patient empowerment supported by ICT

• Nanotechnologies, Advanced Materials and Production

• NMP 6 - 2015: Novel nanomatrices and nanocapsules

• NMP 7 - 2015: Additive manufacturing for table-top nanofactories

• NMP 12 - 2015: Biomaterials for treatment and prevention of Alzheimer‟s disease

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Participation in thematic networks

• European Strategy Forum on Research Infrastructures (ESFRI)

• European technology Platform (ETP) Nanomedicine

• Cost Actions (several topics)