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1.Ocular lens magnifies
10X2. Body tube
3. Arm
4. Revolving nose piece
5. Stage clip
6. Objective lenses magnify 4x, 10x and 40x7. Adjustment
knob8. Slide
9. Stage10. Coarse adjustment knob (low power)13. Fine
adjustment knob
12. Light
14. Power switch15. Base
11. Iris Diaphragm
= total magnification
magnification of ocular lens
x magnification of objective lens
Example:
10 x 40 = 400x
Microscope parts and functions Part Part
##Part Part
NameNamePurpose of PartPurpose of Part
11 Look through this to see an object under the microscope. Usually 10X magnification.
22 Connects the ocular to the microscope.
33 Connects the base and the barrel.
44 Turns to change the objective lenses to use.
55 Holds the slide in place
66 Used to view objects at three different magnifications
77 Moves the stage side-to-side, or forward and back to get the object into the field of view
Ocular lens
Body Tube
Arm
Nose piece
Stage Clip
Objectivelens
Stageadjustment
knob
Microscope parts and functionsPart
#Part
NamePurpose of Part
88 Piece of glass onto which the object to be viewed is placed
99 Holds the slide for viewing
1010 Raises and lowers the stage for focusing under low power
1111 Adjusts the amount of light entering the field of view
1212 Source of light
1313 Slightly moves the stage to sharpen the image
1414 Turns the illumination on or off
1515 Supports the microscope
Slide
Stage
Coarsefocus
Irisdiaphragm
Lamp
FineFocus
PowerSwitch
Base
Vesicle/vacuole - storage
RibosomesMake proteins
Lysosome - digestion
Cell membrane- semi-permeable
Cytoplasm - fluid
Centrioles- Cell division
Nucleus - brain
14. MitochondrionMakes energy (ATP)
Animal Cell
Chromosome - DNA
Nucleolus – makes RNA and ribosomes
Golgi complex - packagesEndoplasmic Reticulum - transport
Bozeman – A tour of the Cell
http://www.youtube.com/watch?v=1Z9pqST72is
From 5:17 to end
Vacuoles
When a plant cell has stopped growing there is usually one very large vacuole. Sometimes that vacuole can take up more
than half of the cell's VOLUME. The vacuole holds large amounts of water or food. The vacuoles can also hold the
plant waste products.
ChloroplastStructure• Contains chlorophyll and enzymes required
for photosynthesis• Contains ribosomes and a small loop of DNA
ChloroplastFunction• Production of
carbohydrates - glucose
6CO2 + 6H2O 6O2 + C6H12O6 (glucose)
• The process of producing these carbohydrates is known as photosynthesis
Cell WallStructure• Found in plant cells, made of layers of
celluloseFunction• Support and protection of the cell
Cell membrane vs. Cell wall
Plant Cell Diagram
Cell wall - cellulose
Nucleus - brain
Nucleolus – makes RNA
Chromosome - DNA
Mitochondrion - energy
Chloroplast - photosynthesis
Golgi complex – packaging
Cell membrane
ribosomes- make proteins
Vacuole - storage
cytoplasm
E.R. - transport
Lysosome - digestion
Plant cell Animal Cell
Both
Cell Wall
chloroplast
Centrioles
vacuolesnucleus
nucleolus
CytoplasmGolgi
complexRibosomeslysosomesmitochondri
acell membranevesicles
E.R.
nucleus
nucleolus
ribosomes
Cell membraneCell wall
cytoplasmGolgi complex
mitochondrion
chloroplast
Vacuole chromosomes
Endoplasmic reticulum
vesicle or lysosome
How To Use a MicroscopeHow To Use a Microscope1. Make sure the objective lens with the lowest
power is in place.
2. Place the slide on the stage and secure the slide using the stage clip.
3. Use the coarse focus knob to bring the objective lens close to the slide. Use the fine focus knob to focus.
4. To move to high power, rotate the nosepiece to the high power objective lens. Use the fine adjustment knob to bring the image into focus.