Embed Size (px)
Citation preview
1999 2005 201020032001 20072000 2002 2004 2006 2008 2009 2011 2012 2013 2014
2005-2006
2006-2007
2007-2008
2008-2009
2009-2010
2010-2011
2011-2012
2012-2013
0500
100015002000250030003500400045005000
Student Participation in Classroom and BIOlab Visits
BIOlab
In-School
In-S
choo
l Vis
itsBI
Ola
b Vi
sits
17312647
2012-2013
22011152
362
217
446 Other schools
Other schools
Why youth?
Programming and Curriculum
• Developed with teachers• Based on MB curriculum• Hands-on, authentic science
• Grade 5: Maintaining a healthy body• Grade 6: Health curriculum• Grade 7: Solutions, ecosystems• Grade 8: Cells and systems, optics, fluids• Grade 9: Reproduction• Grade 11 Biology: Body systems• Grade 12 Biology: Genetics
Lab Safety Basics• If you don’t know what it is,
don’t touch it!• No food or drinks!• Wear safety equipment when
instructed!• Follow instructions!• Ask questions if you don’t
understand instructions!!!
Lab Safety Basics• You must wash your
hands when leaving the lab and after removing your gloves!
• Report any spills immediately!
Lab Safety Basics
• You will be wearing gloves for a reason! DO NOT touch other people or any surfaces when wearing gloves!
Your patient• Patient was brought
in by his wife after he fainted at home.
• He is complaining of severe chest pain.
You listen to his heart:Sound 1Sound 2
Tests
You take his blood pressure:BP= 168/93
EKGYou order an EKG: this can show us if his heart is beating properly.
normal
patient
Echocardiography
Turbulent flow around aortic valve
Left ventricular hypertrophy
Diagnosis: Aortic Stenosis
Normal aortic valveStenotic aortic valve
What now?CT ScanValve replacement surgery
Or could we build him a new valve?
1. Get a donor valve2. Decellularize donor valve3. Get stem cells from patient4. Grow stem cells from patient into the
cell type we want
Stem cellsEmbryonic Adult
Any type of cell!
Some types of cells!
From bone marrow or possible other sites
Stem cell therapy• Grow billions of cells• Push to cell type we want
Cell culture
Muscle cells (myocyte) Fibroblasts and myofibroblasts
DigestResearch
1. Using a dropper, suck out the media (liquid cell food), discard in waste beaker2. Using a clean dropper, cover with PBS and gently wash cells by rocking the dish3. Remove PBS and discard in waste tube. Using a clean dropper each time, do two
more PBS washes and discard. Check cells under scope when done.4. Using a clean dropper, add 2ml trypsin and rock in an ‘X’ pattern until cells are
detached (check on the scope, about 5min)5. Using a clean dropper, add 2ml DMEM and draw up all of the liquid with the
dropper DO NOT PUT INTO THE WASTE CONTAINER!!!6. Put the liquid into a clean, empty 15ml blue capped tube7. Wait for instructions to spin in centrifuge
Working with live cells
Next steps• We hope to partner with your school division!
Division consultants are attending the BIOlab in November, please get in touch with yours to express interest.
• Get in touch with Steve [email protected]• Check out the website www.youthbiolab.ca
