14722_BTY319%20__%20CONCEPTS%20IN%20ANIMAL%20AND%20PLANT%20%20BIOTECHNOLOGY

8/7/2019 14722_BTY319%20__%20CONCEPTS%20IN%20ANIMAL%20AND%20PLANT%20%20BIOTECHNOLOGY

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Lovely Professional University,Punjab

Course No Cours Title Course Planner Lectures Tutorial Practical Credits

BTY319 CONCEPTS IN ANIMAL AND PLANT BIOTECHNOLOGY 14965 :: Amarish Kumar Sharma 3 0 2 4

Sr. No. (Web adress) (only if relevant to the courses) Salient Features

Sr No Jouranls atricles as compulsary readings (specific articles, Complete reference)

Bhojwani, S.S and Razdan M,K, Plant Tissue Culture. Theory and Practice, Elsevier,20061

Culture of Animal Cells, (3rd Edition), F1. Ian Freshney. Wiley-Liss, 20042

Text Book:

Other Specific Book:Singh B D, Biotechnology, Expanding Horizons, Kalyani Publishers,20073

Animal Cell Culture â€“ Practical Approach, Ed. John R.W. Masters, Oxford, 20064

Cell Culture Lab Fax. Eds. M. Butler & M. Dawson, Bios Scientific Publications Ltd., Oxford, 4th edition,20005

Relevant Websites

Other Reading

Detailed Plan For Lectures

Week Number Lecture Number Lecture Topic Chapters/Sections ofTextbook/otherreference

Homework to be Assignedto students

Pedagogical toolDemonstration/casestudy/images/anmation ctc. planned

Format For Instruction Plan [for Courses with Lectures and Labs

1 Approved for Spring Session 2010-11



Part 1Week 1 Lecture 1 Introduction to Plant Biotechnology-(Part-1) ->Reference :1,Pg.1-18

Lecture 2 Introduction to Plant Biotechnology(Part-2)

Lecture 3 Plant Tissue Culture- tools & techniques(Briefoverview)

HomeWork 1 Allocation

Week 2 Lecture 4 Introduction to Animal Biotechnology(Part-1) ->Reference :2,6/1 Pg131-140

Term Paper 1 Allocation

Lecture 5 Introduction to Animal Biotechnology(Part-2).

Lecture 6 Animal Tissue Culture-Tools & Techniques(Briefoverview).

Week 3 Lecture 7 Sterlization Techniques:Overview ->Reference :2,7/3

Lecture 8 Sterilization of Media & Glasswares

Lecture 9 Sterlization Techniques: large Scale Cultures HomeWork 1 Submission

Week 4 Lecture 10 Micropropagation (Part-1) ->Reference:1,pg.264;488-514

Class test based onhomework1

Part 2Week 4 Lecture 11 Micropropagation (Part-2) ->Reference

:1,pg.264;488-514;451HomeWork 2 Allocation http://www.public.iastate

.edu/~lcs/423ppts/lec04.423.07.ppt

Lecture 12 Development of Virus Free Plants

Week 5 Lecture 13 Somaclonal variation-(Part-1) ->Reference:1,9/2Pg.123-128

http://www.public.iastate.edu/~lcs/423ppts/lec08.423.07.ppt

Lecture 14 Somaclonal variation-(Part-2)

Lecture 15 Applications of Somaclonal Variat ion-1 ->Reference:1,19/2Pg.123-128->Reference :1,135-288

Week 6 Lecture 16 Applications of Somaclonal Variation-2 ->Reference :1,pg15;167-213->Reference :1,135-288

HomeWork 2 Submission




Week 6 Lecture 17 Development of Haploid Plants ->Reference :1,pg15;167-213


Lecture 18 Embryo Culture -1 ->Reference :1,pg5;297-335

Week 7 Lecture 19 Embryo Culture -2 ->Reference :1,->Reference 1pg 5;297-335

Lecture 20 Embryo Rescue -Concept & application

Lecture 21 Revision

MID-TERMPart 3

Week 8 Lecture 22 Somatic Hybrids ->Reference :1,10/2Pg.129-143->Reference :1,293-395

Lecture 23 Cybrids HomeWork 3 Allocation

Lecture 24 Germaplasm conservation (Part-1) ->Reference :1,->Reference :1,293-395

Week 9 Lecture 25 Germaplasm conservation (Part-2) ->Reference :1,3/1 Pg41-52

Lecture 26 Molecular Markers -Function & Mechanistic role ->Reference :1,310-389

Lecture 27 Molecular Markers -Types and their respectivefunction

->Reference :2,2310-389->Reference :2,2225/2Pg 435-450

Week 10 Lecture 28 Gene markers,DNA markers,Protein markers ->Reference :2,225/2Pg 435-450->Reference :2,2310-389

HomeWork 3 Submission

Lecture 29 Culture Media for animal cells. ->Reference :2,310-389->Reference :2,225/2Pg 435-450







Homework No. Topic of the Homework Nature of homework(group/individuals/field work

Homework 1 Introduction to plant and Animal Biotechnology,Sterlization Techniques: Media, Glassware, Tissues andtheir large Scale Cultures.

Group

Homework 2 Micropropagation and Virus â€“ Free Plants,Somaclonal variation Group

Homework 3 Somatic Hybrids and Cybrids, Germaplasm conservation

Molecular Markers

Haploid Plants, Embryo Culture and Embryo Rescue

Group

Homework 4 Culture Media for animal cells. Cell culture and Organ culture.

Somatic Cell Fusion, Hybridoma Technology

Initiation Of Cell Cultures, Evolution of Cell Lines

Group

Scheme for CA:out of 100*Component Frequency Out Of Each Marks Total Marks

Homework 3 4 5 15

Class test based on homework 3 4 10 30Term Paper 1 25 25

Lab Assesment 30 30

Total :- 70 100

* In ENG courses wherever the total exceeds 100, consider x best out of y components of CA, as explained in teacher's guide available on theUMS

List of suggested topics for term paper[at least 15] (Student to spend about 15 hrs on any one specified term paper)

Sr. No. Topic

1 Introduction to plant biotechnology.

2 Introduction of animal technology

3 Sterilization technique -Plant Tissue Culture

4 Sterilization technique -Animal Tissue Culture

5 Micropropagation




6 Development of Virus free plants

7 Somaclonal Variation

8 Haploid Plants

9 Embryo Culture

10 Embryo Rescue

11 Somatic hybrids

12 Somatic Cybrids

13 Germplasm Conservation14 Molecular Markers

15 Hybridoma Technology

*Each experiment of the lab will be evaluated using following relative scheme:

Component % of Marks

J/E 20

WR 50

VIVA 30

List of experiments :-

LectureNumber

Lecture Topic Pedagogical Tools Or Equipment Planned lab Manual

Group 1 Laboratory setup for Animal and Plant Cell culture Laminar hood,centrifuge,deep-freezer,water-bath,air-filters,weighing balance,ph-meter, microscope,liquidnitrogen tank...

LMBTY319

Group 2 Plugging and seal ing of cul tures Test tube,Bunsen Burner,Inoculation loop,Laminarhood

LMBTY319

Group 3 Preparation and sterilization of medium,MS media Autoclave,flask,weighing balance LMBTY319

Group 4 Sterilization of explants and their culture Petri-plate,laminar hood LMBTY319

Group 5 Preparation and sterilization of Animal cell culturemedia.

Autoclave,flask,weighing balance LMBTY319




Mid TermGroup 6 Revival of stored cells and cell lines. Laminar hood,centrifuge,deep-freezer,water-

bath,weighing balance,ph-meter,microscopeLMBTY319

Group 7 Culture of animal cells and their observation undermicroscope

Laminar hood,centrifuge,deep-freezer,water-bath,air-filters,weighing balance,ph-meter,microscope

LMBTY319

Group 8 Cell splitting and cell count, using haemocytometer. Haemocytometer,Microscope,cover-slip. LMBTY319

Group 9 DNA extraction from plant and animal cells Thermocycler,,Centrifuge,Laminar hood,WaterBath,Micro-pipettes,Freezer

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Group 10 Polymerase chain reaction Thermocycler,PCR Reagents,Centrifuge,Laminarhood,Water Bath,Micro-pipettes,freezer

LMBTY319

Spill OverGroup 11 Polymerase chain reaction Thermocycler,PCR Reagents,Centrifuge,Laminar

hood,Water Bath,Micro-pipettes,freezerLMBTY319


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14722_BTY319%20__%20CONCEPTS%20IN%20ANIMAL%20AND%20PLANT%20%20BIOTECHNOLOGY