Protein Peptide Fragment Number Start End ModifiersPTH1-34 SVSEIQLMHNLGK 1:T001 1 13PTH1-34 SVSEIQLMHNLGK 1:T001-H2O 1 13PTH1-34 SVS 1:T001/b3 1 3PTH1-34 SVSE 1:T001/b4 1 4PTH1-34 SVSE 1:T001/b4-H2O 1 4PTH1-34 EIQLMHNLGK 1:T001/y10 4 13PTH1-34 EIQLMHNLGK 1:T001/y10-H2O 4 13PTH1-34 SEIQLMHNLGK 1:T001/y11 3 13PTH1-34 SEIQLMHNLGK 1:T001/y11-H2O 3 13PTH1-34 VSEIQLMHNLGK 1:T001/y12 2 13PTH1-34 VSEIQLMHNLGK 1:T001/y12-H2O 2 13PTH1-34 LGK 1:T001/y3 11 13PTH1-34 NLGK 1:T001/y4 10 13PTH1-34 HNLGK 1:T001/y5 9 13PTH1-34 MHNLGK 1:T001/y6 8 13PTH1-34 MHNLGK 1:T001/y6-H2O 8 13PTH1-34 LMHNLGK 1:T001/y7 7 13PTH1-34 QLMHNLGK 1:T001/y8 6 13PTH1-34 QLMHNLGK 1:T001/y8-NH3 6 13PTH1-34 IQLMHNLGK 1:T001/y9 5 13PTH1-34 IQLMHNLGK 1:T001/y9-H2O 5 13PTH1-34 HLNSMER 1:T002 14 20PTH1-34 HLNSMER 1:T002 14 20PTH1-34 HL 1:T002/b2 14 15PTH1-34 HLN 1:T002/b3 14 16PTH1-34 HLNSME 1:T002/b6 14 19PTH1-34 MER 1:T002/y3 18 20PTH1-34 SMER 1:T002/y4 17 20PTH1-34 NSMER 1:T002/y5 16 20PTH1-34 LNSMER 1:T002/y6 15 20PTH1-34 LNSMER 1:T002/y6 15 20PTH1-34 VEWLR 1:T003 21 25PTH1-34 VEWLR 1:T003 21 25PTH1-34 VEWLR 1:T003-H2O 21 25PTH1-34 VE 1:T003/b2 21 22PTH1-34 WLR 1:T003/y3 23 25PTH1-34 VEWLR 1:T003x2 21 25PTH1-34 K 1:T004 26 26PTH1-34 LQDVHNF 1:T006 28 34PTH1-34 LQDVHNF 1:T006* 28 34 Amidation C-TERM(1)PTH1-34 LQDVHNF 1:T006* 28 34 Amidation C-TERM(1)PTH1-34 LQDVH 1:T006*/a5 28 32PTH1-34 LQDVHN 1:T006*/a6 28 33PTH1-34 LQ 1:T006*/b2 28 29PTH1-34 LQD 1:T006*/b3 28 30PTH1-34 LQDVHN 1:T006*/b6 28 33PTH1-34 HNF 1:T006*/y3* 32 34 Amidation C-TERM(1)

PTH1-34 HNF 1:T006*/y3-NH3* 32 34 Amidation C-TERM(1)PTH1-34 VHNF 1:T006*/y4* 31 34 Amidation C-TERM(1)PTH1-34 VHNF 1:T006*/y4* 31 34 Amidation C-TERM(1)PTH1-34 VHNF 1:T006*/y4-NH3* 31 34 Amidation C-TERM(1)PTH1-34 DVHNF 1:T006*/y5* 30 34 Amidation C-TERM(1)PTH1-34 DVHNF 1:T006*/y5-NH3* 30 34 Amidation C-TERM(1)PTH1-34 QDVHNF 1:T006*/y6* 29 34 Amidation C-TERM(1)PTH1-34 QDVHNF 1:T006*/y6-NH3* 29 34 Amidation C-TERM(1)PTH1-34 LQDVHNF 1:T006*x2 28 34 Amidation C-TERM(1)PTH1-34 LQDVHNF 1:T006-NH3* 28 34 Amidation C-TERM(1)PTH1-34 LQ 1:T006/b2-NH3 28 29PTH1-34 LQDVHN 1:T006/b6-NH3 28 33

Calculated Peptide Mass (Da) b/y Possible Control RT (Min) Control m/z1454.7551 24 3.2 728.38471436.7446 24 3.2 479.9246

273.1325 4 3.2 274.1348402.175 6 3.2 403.1772

384.1645 3 3.2 385.16741181.6227 18 3.2 591.81811163.6121 18 3.2 582.8121268.6547 20 3.2 635.3321250.6442 20 3.2 626.32511367.7231 22 3.2 684.87411349.7125 22 3.2 450.9113

316.2111 4 3.2 317.2135430.254 6 3.2 431.2514

567.3129 8 3.2 568.3054698.3534 10 3.2 699.3493680.3428 10 3.2 681.3425811.4374 12 3.2 812.4296

939.496 14 3.2 940.493922.4695 14 3.2 462.2407

1052.5801 16 3.2 527.29571034.5695 16 3.2 518.2859

885.4127 12 1.8 443.7069885.4127 12 2.5 443.7062

250.143 2 1.8 251.147364.1859 4 1.8 365.1844

711.301 10 1.8 712.3019434.1948 4 1.8 435.1898521.2268 6 1.8 522.2272635.2697 8 1.8 636.2714748.3538 10 1.8 749.3521748.3538 10 2.5 749.3524

701.386 8 3.3 702.3866701.386 8 4 702.3879

683.3755 8 3.3 342.6983228.111 2 3.3 229.1124

473.2751 4 3.3 474.2711402.7721 8 3.3 1403.7737

146.1055 0871.4188 12870.4348 12 2.9 871.4304870.4348 12 2.8 871.4443

564.302 8 2.9 565.2967678.3449 10 2.9 679.3409241.1426 2 2.9 242.1469356.1696 4 2.9 357.1679706.3398 10 2.9 354.1693415.1968 4 2.9 416.1936

398.1703 4 2.9 399.1724514.2652 6 2.9 515.2604514.2652 6 2.8 515.2662497.2387 6 2.9 498.2412629.2922 8 2.9 630.2862612.2656 8 2.9 613.2614757.3508 10 2.9 758.3468740.3242 10 2.9 741.3219

1740.8696 12 2.9 1741.8632853.4083 12 2.9 427.7054224.1161 1 2.9 225.1212689.3133 5 2.9 345.6568

3.3 603.31515.5 398.23182.8 500.26565.5 376.24893.6 1275.63267.1 282.2723.6 906.44773.5 705.87315.5 773.47973.4 758.40093.3 585.30053.3 1082.04142.8 418.21712.9 413.70722.8 361.19583.4 656.33652.8 491.7552.9 724.29943.3 288.19382.8 584.33141.8 304.1536.9 256.25782.9 466.19571.8 223.14832.9 577.2314

3 643.33073.5 540.28232.9 594.26033.2 458.21713.2 491.75933.4 612.82193.4 648.32397.1 265.24833.6 548.29773.4 749.39143.2 628.3252

3.2 721.3813.2 754.85193.6 858.0922.8 347.19893.3 201.11452.9 352.15546.5 228.23053.4 752.39593.4 838.79793.2 330.15893.6 697.86042.8 485.26253.2 571.2998

3 736.38833.2 1066.5257.1 247.23892.9 419.19733.3 272.17233.2 217.0743

6 281.16622.9 585.26941.8 347.17383.6 868.4037.1 304.25592.8 477.75734.7 213.14413.3 316.12212.9 480.21463.3 734.39282.9 463.18192.9 405.19583.2 1043.51673.2 440.22092.9 696.30833.6 990.46313.3 298.11493.1 577.28881.8 470.16866.5 233.18682.8 567.31262.1 647.34583.6 844.75923.2 739.3932.8 556.33983.2 242.1472.8 352.17541.8 434.69277.3 284.2915

3.2 1123.54362.8 468.24112.8 526.72853.2 953.45053.4 695.37423.3 717.37133.4 669.34992.8 409.70677.1 270.27432.9 713.33443.2 839.40563.3 739.33943.4 624.82322.8 747.3087

7 550.61993.4 662.34452.8 707.34386.2 537.3363.2 857.41676.3 449.28283.2 662.29842.7 402.23832.8 252.1083.2 509.77642.1 576.30161.8 454.69511.9 633.31632.9 262.11513.6 571.32863.3 411.20063.2 617.32333.1 621.3192.8 594.26252.9 234.12063.6 901.45562.9 252.10795.5 348.11722.9 455.19562.9 924.35813.5 719.38523.5 618.82356.3 493.3063.3 732.36813.2 608.30543.1 635.3098

6 356.12922.7 572.34763.7 776.3771

3.2 241.22483.4 659.85933.2 578.78683.3 300.16652.8 416.19683.2 775.0855.5 393.28

4 726.3611.8 452.22072.9 410.18633.3 427.72895.5 421.31073.6 644.32233.6 749.35612.8 343.15522.8 539.31583.2 747.36432.8 758.35763.6 516.26313.4 582.81093.6 403.17612.8 354.1512.9 462.6679

4 855.40823.2 347.18763.3 793.76535.3 409.15912.8 439.25753.4 600.7833.2 543.30763.6 863.41492.9 526.23684.1 770.38692.8 404.22242.9 340.14733.2 519.27613.4 960.51322.8 338.68682.8 577.23592.8 511.26072.8 480.2142.9 893.41763.3 670.33383.2 516.26161.8 478.23582.9 244.08983.2 791.3715

4 477.7356

3.2 731.00113.1 568.28312.9 549.23932.9 768.32993.2 596.80692.8 463.19563.2 771.91333.4 1064.04743.2 638.07592.8 741.3359

3 510.75182.6 523.79933.3 716.03167.1 585.53043.2 713.0453.8 744.39762.9 438.21522.8 483.24633.2 1141.57182.8 399.173.6 561.3083.4 684.86863.6 757.40743.2 496.23642.8 395.7092.6 636.273.4 688.37063.4 719.38473.4 587.82983.4 766.07352.9 662.32483.5 734.3873.2 1482.73962.8 519.24285.5 751.5093.2 606.82493.1 523.93793.3 713.87823.3 605.73142.9 641.26363.3 803.862

3 611.33473.4 777.38682.8 679.30133.1 528.62462.8 704.37262.8 549.25543.6 928.4461

3.1 714.37493.3 731.72943.5 716.40243.2 654.31183.5 717.3682.8 449.71933.2 971.18183.4 776.37343.2 641.01983.1 671.84883.4 708.42283.4 1007.50533.2 537.9429

3 535.2931

Control Charge State Control Mass (Da) Control Intensity (Counts) Control b/y Found2 1454.7535 728805 193 1436.75 18640 131 273.1269 4396 01 402.1693 2860 01 384.1595 2683 02 1181.6202 26790 122 1163.608 8233 72 1268.6482 271903 142 1250.6343 21201 92 1367.7323 28347 83 1349.71 1922 11 316.2056 3317 01 430.2435 8707 21 567.2975 18666 11 698.3414 24633 71 680.3346 882 01 811.4217 28160 81 939.4851 35697 112 922.4655 15478 62 1052.5754 44011 102 1034.556 1031 02 885.398 232539 72 885.3965 5380 01 250.1391 19142 01 364.1765 3378 11 711.294 2135 31 434.1819 8698 21 521.2193 5684 31 635.2635 52522 61 748.3441 43957 51 748.3445 549 01 701.3787 217054 31 701.38 2222 02 683.3807 1189 01 228.1045 10082 01 473.2631 139544 21 1402.7657 959 0

1 870.4225 232792 91 870.4364 1638 41 564.2888 2505 21 678.333 1287 01 241.139 12098 01 356.16 6048 22 706.3227 29931 71 415.1857 13225 1

1 398.1645 3789 11 514.2525 9063 31 514.2583 1306 01 497.2333 2350 11 629.2783 65946 51 612.2535 23912 51 757.3389 65922 61 740.314 57902 61 1740.8552 926 02 853.3949 22280 91 224.1133 3514 02 689.2978 4430 21 602.3072 2704451 397.2239 962022 998.5154 911491 375.241 772992 2549.2493 444331 281.2641 376941 905.4398 317602 1409.7303 308881 772.4718 300353 2272.179 291151 584.2926 252992 2162.0669 231002 834.4184 220262 825.3985 216342 720.3758 209902 1310.6571 174542 981.4941 173131 723.2915 171331 287.1859 165651 583.3235 164931 303.1451 149471 255.2499 128191 465.1878 115671 222.1404 113701 576.2235 105872 1284.6455 104122 1078.5488 101521 593.2524 99821 457.2092 98242 981.5028 95272 1223.6279 92012 1294.6318 88891 264.2404 87922 1094.5796 87482 1496.7668 86882 1254.6345 8617

2 1440.7462 84512 1507.6879 82573 2571.2522 80142 692.382 79671 200.1066 77901 351.1475 77531 227.2226 76163 2254.1641 72043 2513.3699 71771 329.151 69032 1393.7048 68851 484.2546 67221 570.2919 66172 1470.7607 64271 1065.5171 64271 246.231 62882 836.3787 58551 271.1644 57011 216.0664 56491 280.1583 56201 584.2615 55091 346.1659 54813 2602.1851 52901 303.248 52852 953.4987 51961 212.1362 50451 315.1142 47331 479.2067 47162 1466.7698 46711 462.174 46592 808.3758 46211 1042.5088 45841 439.213 45841 695.3004 45452 1978.9103 44221 297.107 42112 1152.5616 41862 938.3214 40391 232.1789 39631 566.3047 39471 646.3379 39243 2531.2537 39032 1476.7701 38801 555.3319 38751 241.1391 38052 702.3349 37631 433.6848 35401 283.2836 3529

1 1122.5356 34521 467.2332 34072 1051.441 33871 952.4426 32482 1388.7325 30702 1432.7268 30652 1336.6838 30462 817.3975 29751 269.2664 29381 712.3264 29161 838.3977 28943 2214.9944 28624 2495.2612 28392 1492.6016 28251 549.612 28212 1322.6731 26591 706.3359 26171 536.3281 26121 856.4088 26061 448.2749 25552 1322.5809 25371 401.2304 25181 251.1001 25152 1017.5369 25071 575.2937 24942 907.3743 24601 632.3084 24541 261.1072 24461 570.3207 24261 410.1927 23872 1232.6307 23582 1240.6221 23051 593.2546 22971 233.1127 22902 1800.8954 22801 251.1 22711 347.1093 22632 908.3754 22481 923.3502 22452 1436.7545 22142 1235.6312 22111 492.2981 22063 2194.0806 21841 607.2975 21821 634.3019 21631 355.1213 21461 571.3397 21102 1550.7384 2106

1 240.2169 20752 1317.7026 20602 1155.5577 20541 299.1586 20461 415.1889 20383 2322.2312 20331 392.2721 20041 725.3531 19901 451.2128 19782 818.3568 19702 853.4419 19691 420.3028 19451 643.3144 19341 748.3482 19291 342.1473 19191 538.3079 19162 1492.7128 19011 757.3497 18911 515.2552 18832 1163.6058 18701 402.1682 18451 353.1431 18321 461.66 18131 854.4003 17721 346.1797 17703 2378.272 17701 408.1512 17191 438.2496 16872 1199.5502 16681 542.2997 16663 2587.2209 16581 525.2289 16561 769.379 16562 806.429 16492 678.2787 16442 1036.5364 16241 959.5053 16012 675.3577 15981 576.228 15852 1020.5055 15751 479.2061 15691 892.4097 15673 2007.9777 15461 515.2537 15401 477.2279 15121 243.0819 14921 790.3636 14852 953.4554 1477

3 2189.9795 14562 1134.5504 14471 548.2314 14321 767.322 14262 1191.5979 14081 462.1877 14042 1541.8108 13762 2126.0789 13703 1911.2037 13491 740.328 13272 1019.4877 13192 1045.5826 13143 2145.0708 13091 584.5225 13083 2136.1113 13031 743.3897 12931 437.2073 12702 964.4767 12591 1140.5638 12231 398.1621 12072 1120.6002 12022 1367.7213 11891 756.3995 11521 495.2285 11402 789.4021 11071 635.2621 10922 1374.7253 10892 1436.7535 10842 1173.6437 10783 2295.1968 10481 661.3169 10382 1466.7581 10262 2963.4634 10092 1036.4697 9951 750.5011 9942 1211.6339 9853 1568.7898 9762 1425.7405 9684 2418.8938 9671 640.2557 9644 3211.4163 9642 1220.6534 9622 1552.7578 9561 678.2934 9543 1582.85 9381 703.3647 9221 548.2475 9201 927.4382 902

2 1426.7339 9003 2192.1643 8731 715.3945 8202 1306.6078 7932 1432.7202 7902 897.4227 7623 2910.5215 7273 2326.0962 7163 1920.0356 7102 1341.6818 6992 1414.8297 6842 2012.9948 6023 1610.8048 5722 1068.5703 564

Control Assigned Intensity (%) Control b/y %43.6 79.2

9.3 54.20 0

0.5 00.6 019 66.7

4.7 38.930.1 7015.9 4519.5 36.4

0.1 4.50 0

12.5 33.34.6 12.5

23.9 700 0

18.8 66.728.4 78.6

9.2 42.923.2 62.5

0 055.7 58.3

0 01.9 0

20.9 255.1 306.1 50

11.7 5018.4 7534.1 50

0 036.1 37.5100 0

0 00 0

21.1 500 0

17.9 7525.9 33.3

8.1 250 0

2.2 011.5 5022.5 70

9.6 25

4.4 2518.8 50

0 02.8 16.7

15.8 62.515.1 62.513.8 6011.9 60

0 020.2 75

5.9 07.4 40

Control b/y List Control Mab3;b4;b5;b6;b7;b8;b9;b10;b11;b12;y2;y3;y4;y6;y7;y9;y10;y11;y12 -1.1b3;b4;b5;b6;b7;b8;b9;b10;b11;b12;y3;y10;y12 3.8

-20.5-14.2

-13y2;y3;y4;y6;y9;(y10b3);(y10b4);(y10b5);(y10b6);(y10b7);(y10b8);(y10b9) -2.1(y10b3);(y10b4);(y10b5);(y10b6);(y10b7);(y10b8);(y10b9) -3.5y2;y3;y4;y6;y7;y9;y10;(y11b2);(y11b3);(y11b4);(y11b7);(y11b8);(y11b9);(y11b10) -5.1y3;y4;y10;(y11b3);(y11b4);(y11b7);(y11b8);(y11b9);(y11b10) -7.9y2;y3;y4;y6;y9;y10;y11;(y12b6) 6.7(y12b6) -1.9

-17.4y3;(y4b2) -24.4y3 -27.1y2;y3;y4;(y6b2);(y6b3);(y6b4);(y6b5) -17.2

-12.1y2;y3;y4;y6;(y7b2);(y7b3);(y7b4);(y7b6) -19.3y2;y3;y4;y6;y7;(y8b2);(y8b3);(y8b4);(y8b5);(y8b6);(y8b7) -11.6y3;(y8b3);(y8b4);(y8b5);(y8b6);(y8b7) -4.3y2;y3;y4;y6;y7;(y9b2);(y9b3);(y9b5);(y9b6);(y9b7) -4.5

-13b2;b3;b6;y2;y3;y4;y6 -16.6

-18.3-15.6

(y6b2) -25.8b3;(y2b6);(y5b4) -9.8y2;(y2b6) -29.7y2;y3;(y4b2) -14.4y2;y3;y4;(y5b2);(y5b3);(y5b4) -9.8y2;y3;y4;(y6b2);(y6b3) -13

-12.4b2;b3;y2 -10.4

-8.67.6

-28.5y2;(y3b2) -25.4

-4.6

b2;b3;b4;b5;b6;y2*;y4*;y5*;y6* -14.1b4;b5;b6;y6* 1.8b2;b4 -23.4

-17.5-14.9

b2;(y6b2) -27b2;b3;b4;b5;(y2b6);(y3b6);(y4b6) -24.2y2* -26.7

y2* -14.6y2*;(y3b6);(y4b2) -24.7

-13.4y2* -10.9y2*;y4*;(y4b6);(y5b2);(y5b3) -22.1y2*;y4*;(y4b6);(y5b2);(y5b3) -19.8y2*;y4*;y5*;(y6b2);(y6b3);(y6b4) -15.7y2*;y4*;y5*;(y6b2);(y6b3);(y6b4) -13.8

-8.3b2;b3;b4;b5;b6;y2*;y4*;y5*;y6* -15.7

-12.5b2;b4 -22.5

Control % Analyte RT Analyte m/Analyte Ch Analyte MaAnalyte IntAnalyte b/ Analyte AssAnalyte b/97.5

2.5100

51.648.476.523.592.8

7.293.7

6.3100100100

96.53.5

10069.830.297.7

2.397.7

2.3100100100100100100

98.81.298

10.5

1001000.4

90.40.6

1003.6

77.5100

8477.7

22.371.310.318.573.426.653.246.8

0.48.6

22.512.4

Analyte b/yAnalyte MaAnalyte % Delta MassDelta IntenDelta RT ( Comment0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0

0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0

0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0

0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0

0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0

0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0

0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0

0 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 00 0 0