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1
Semiochemical Mediated Host Plant Preference in the Yellowmargined Leaf beetle, Microtheca ochroloma
Rammohan Rao Balusu and Henry Fadamiro
Department of Entomology and Plant Pathology, Auburn University
2
Introduction
Yellowmargined leaf beetle (YMLB), Microtheca ochroloma
(Chrysomelidae) is a pest of cruciferous (Brassicaceae) crops
Cabbage, Turnips, Mustard, Raddish, Collards, etc
Native to South America
First reported in the US
in Mobile, AL in 1947
A serious pest the SEA major problem facing
organic vegetable production
Damage
Both adults and larvae feed on foliage by first making small holes; later serious defoliation
Defoliation of larvae on turnip Mass attack of adults on napa cabbage
Organically-managed crucifer field in south Alabama damaged by YMLB
TurnipNapa cabbage
Mustard greensNapa cabbage
Tet soi B. narinosa
Mizuna B. rapa japonica
TurnipsRadish
TurnipsCollards
LettuceCabbage
Mixed cropping practice of different crucifer vegetables in snow’s bend organic farm in Alabama
Investigate mechanism of host plant selection and preference in yellowmargined leaf beetle (YMLB)
• Field observations suggest preference among crucifer plants
• Questions:– Do YMLB adults show preference among crucifer plants? – What cues mediate host preference?
• Hypothesis:– Host preference is mediated by plant semiochemicals
Objective
3 ft
4 ft
4 ft adult beetles
50Turnip
Cabbage
Collards
Napa cabbage
Materials and methods
Greenhouse and laboratory experiments conducted to test host preference
Q. Feeding and Ovipositional preference?
• Four host plants compared: – cabbage, collards, napa cabbage and turnip
• Experiment (simultaneously) replicated three times (i.e. 3 cages)
• Data collected: – every day for the period of three weeks– number of adults and larvae per plant (up to 21 days) – damage ratings of plants
• Damage ratings based on a scale of 1 to 6: 1 = very light defoliation (< 10%) 2 = light defoliation (10-30%) 3 = moderate defoliation (30-50%)4 = heavy defoliation (50-70%) 5 = very heavy defoliation (70-90%) and 6 = complete (total) defoliation (> 90%)
• Statistical analysis: ANOVA + Tukey HSD test
Materials and methods – greenhouse study
Day after release
No.
of
Ad
ult
s p
er p
lan
t
0
10
20
30
40
TurnipNapa CabbageCabbageCollards
1 2 3
a
b
cc
a
b
c
a
b
Results: Number of YMLB adults per host plant per day
Results: Average damage rating caused by YMLB per plant per week
Week0 1 2 3 4
Dam
age
rati
ngs
per
pla
nt
0
1
2
3
4
5
6
7
TurnipNapa CabbageCabbageCollards
a
b
a
b
a
b
One week after Two weeks after
Results
Laboratory bioassay with four-choice olfactometer
• 10 adults (2-4 days old) per replications
• 20 replicates per experiment per sex
Female
Mea
n(±S
E)
no. o
f re
spon
der
s
0
1
2
3
4
5TurnipNapa CabbageCabbageControl
Male
b
a
cc
b
a
c c
Behavioral response of adult YMLB to different crucifer host plants
Female
Mea
n(±
SE
) no
. of
resp
ond
ers
0
1
2
3
4Turnip Napa CabbageCollardsControl
Male
aba
bc
c
a a
b
b
Behavioral response of adult YMLB to different crucifer host plants
Summary
• Turnip and napa cabbage are preferred host plants over collard and cabbage
• Olfactory cues play a major role in host plant selection
• Trap crop??????
• Ongoing study is focused on chemistry host plants to identify the possible attractant for YMLB
Acknowledgement
• Undergraduate students– Allison Tyler
– David Appel
– Shelia Boyt
• Alabama Agricultural Experiment Station for a seed grant to HYF
Thank you..
Questions?