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Research/ Laboratory Standard Operating Procedure
This is a TEMPLATE ONLY until all applicable appendices and/or UAA Chemical SOPs are attached and the completed document signed by the PI and an EHSRMS&EM designee.
Click here to Enter Procedure or Experiment Title, Chemical name or Class of chemical
#1 Laboratory InformationDepartment Click here to enter text.
Principle Investigator(s) Click here to enter PI name(s); can list more than one for multidisciplinary projects
Location(s) covered by this SOP Click here to enter building(s), room(s)
Creation date Click here to enter a date.SOP Author Click here to enter text.
Revision date Click here to enter a date.
#2 Type of SOP☐ Specific laboratory procedure or experiment
(Examples: synthesis of chemiluminescent esters, folate functionalization of polymeric micelles)☐ Generic laboratory procedure
(Examples: distillation, RNA extraction)☐ Generic use of specific chemical or class of chemicals with similar hazards
(Examples: Ethidium bromide, mineral acids)
#3 Brief Description of Procedure or ExperimentClick here to enter text. Describe what the purpose of the procedure or experiment. Do not include step-by-step instructions here.
#4 Hazard Assessment - Equipment/ Mechanical / Electrical HazardClick here to enter text. Describe all equipment that poses a hazard such as power tools, cutting instruments, needles, scalpel blades, etc.
Hazard MitigationClick here to enter text. Describe what engineering controls and PPE are required to minimize exposure to the above hazards.
#5 Hazard Assessment – Biological, Chemical, RadiationBiological Hazard ☐ Not applicable
Micro-organisms ☐ BSL1 ☐ BSL2Body Fluids/ Tissues ☐ Animal ☐ Human
Lab SOP Page 1 of 20 June 2017
Complete a copy of Appendix B - Biological Material Hazards Assessment form, for each hazard class checked above.Radiation Hazard ☐ Not applicable
☐ Non-ionizing (microwave, infrared, laser, ultraviolet)Eye Protection: Click here to enter text. Specify what type of eye protection is required for the above checked hazard.
☐ Ionizing - Type: ☐ Alpha emitter ☐ Beta emitter ☐ Gamma emitter ☐ X-ray
If any of the ionizing radiation boxes are checked, click on the link below and fill out the UAA Radiation Use Request Form. Contact the Radiation Safety Officer for assistance – 907-786-1268 or [email protected] orhttps://www.uaa.alaska.edu/research/office-of-research-integrity-and-compliance/radiation/
Chemical HazardList all chemicals and reagents used in this SOP. Copy and insert more rows as needed.
Chemical/ Reagent name CAS# (if none, enter ‘see SDS’)
GHS Non-hazardous?
Y or N
Complete a copy of Appendix C - Chemical Hazard Assessment form for EACH hazardous chemical (GHS non-hazardous listed as N or No) listed above. Where available, UAA Chemical Hazard Assessments may be used in place of the Appendix C.
#6 Step-by-Step Operating ProcedureFor each step’s description, include any step-specific hazard, PPE, engineering controls and designated work area in the left-hand column (under Step-by-Step Description).
Guidance on Engineering and Ventilation Controls – Review safety literature and peer-reviewed journal articles to determine appropriate engineering and ventilation controls for your process or experiment.
Guidance on Personal Protective Equipment – To ensure gloves are compatible with and will provide protection from the chemicals in your SOP, refer to the EHSRSM&EM website under ‘Laboratory Safety’ or the glove selection chart from the links below:
www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdfORhttp://vwrcatalog.vwr.com/app.php?RelId=6.4.7.1Click on ‘search’ enter ‘glove chart’ and select the second item
Lab SOP Page 2 of 20 June 2017
Designated work area(s) – Required whenever carcinogens, highly acutely toxic materials or reproductive toxins are used. The intent of a designated work area is to limit and minimize possible sources of exposure to these materials. The entire laboratory, a portion of the laboratory or a laboratory fume hood or bench may be considered a designated work area. This area MUST be wiped down following EACH use to minimize possible exposure.
Procedure - Step-by-Step Description Potential Risks if Step is Not Done or Done Incorrectly (if any)
1. Don personal protective equipment Risk of exposure to any hazards present
2. Check the location/ accessibility/ certification of safety equipment that serves your lab. Notify the PI/ RLS of any deficiencies.
Equipment may malfunction and not provide expected protection, posing a risk of exposure
3. Describe the next step in the procedure Describe what could happen if an unexpected event occurred.
4. Describe the next step in the procedure. Insert additional rows in table, as needed.
Describe what could happen if an unexpected event occurred.
5. Dispose of hazardous solvents, solutions, mixtures, and reaction residues as hazardous waste. See Waste Disposal section for more information.
Describe what could happen if an unexpected event occurred.
6. Clean up work area and lab equipment.Describe specific cleanup procedures that must be performed after completion of your process or experiment. For carcinogens and reproductive toxins, designated areas must be immediately wiped down following each use.
Describe what could happen if an unexpected event occurred.
7. Remove PPE and wash hands. Cross-contamination of biological and chemical materials to surfaces outside of the lab
#7 Emergency Procedures
Health-Threatening EmergenciesFire, explosion, health-threatening hazardous material spill or release, compressed gas leak, valve failure, etc.
CALL 911Alert people in the immediate vicinityPull Fire AlarmEvacuate building and go to your Emergency Assembly Area: Click here to enter text.
Indicate EAA location(s) for the building(s) listed for this SOPRemain nearby to advise emergency respondersOnce personal safety is established, call EHSProvide local notifications (see Emergency Phone Numbers)
Lab SOP Page 3 of 20 June 2017
Note: For compressed gas leaks, shut off gas supply ONLY if this can be done safely, without risk to personnel.
Injuries and ExposuresRemove the injured/ exposed individual from the area, unless it is unsafe to do so because of the medical condition of the victim or the potential hazard to rescuers.Call 911 if immediate medical attention is needed.Call EHS to report the exposure (see Emergency Phone Numbers)Administer first aid as appropriate.Flush contamination from eyes/ skin using the nearest emergency eyewash/shower for a minimum of 15 minutes. Remove any contaminated clothing. Fire blankets and sweat suits are available in every laboratory for use as needed.Bring to the hospital copies of SDSs for all chemicals the victim was exposed to.
Non-Health-Threatening EmergenciesInjuries and Exposures
Flush contamination from eyes/skin for a minimum of 15 minutes.Administer appropriate first aid.Go to EHS website to report the incident on Origami.
Small/ manageable spills In the event of a minor spill or release that can be safely cleaned up using laboratory PPE and spill kit (Amphomag), spills or releases which have been contained within the laboratory (via secondary containment, in a fume hood, on a bench or the floor), spills of less than 500 mL of any flammable material, or spills of materials that do not require respiratory protection for cleanup:
Notify personnel in the area and keep people away from the area. Eliminate all sources of ignition.Protect yourself:
Review the SDS for the spilled material, or use your knowledge of the hazards of the material to determine the appropriate level of protection. Wearing appropriate PPE, clean up the spill with Amphomag following the directions provided in the spill kit. Collect spill cleanup materials in a tightly closed container or double plastic bags. Manage spill cleanup debris as hazardous waste. Contact EHS for waste pickup.Dispose of broken glass in a broken glass container – DO NOT put other materials in the broken glass container.
DO NOT clean up spills requiring respiratory protection – CALL EHS.
Large/ unmanageable spillsFor highly reactive, highly acute toxin, spills or releases which have impacted the environment (via sink / sewer system, soil or air outside the building), spills larger than 500mL of any flammable material, or spill of any material requiring respiratory protection for cleanup:
Protect yourself and others:Contact PI/ RLS and EHS personnel on Emergency Phone Number list.
Lab SOP Page 4 of 20 June 2017
Alert people in the vicinity of the spill, advise them to evacuate the immediate area.Restrict access to the area of the spill. Eliminate all sources of ignition on your way out.Evacuate to a safe area. Remain nearby to advise PI and EHS.
Lab-Specific (Biological/Chemical/Radiation Hazard specific) ProceduresThis section is for any emergency procedures different from standard responses, or for additional emergency
information due to the nature of materials or task. Include information on gas leaks, chemical spills, and personal exposure / medical emergency as appropriate (e.g. Calgonate gel for hydrofluoric acid exposure).
#8 Decontamination/ Waste Disposal and Pollution PreventionDecontaminate disposable items (e.g. pipet tips, plates) and empty chemical containers by triple rinsing with a liquid that will dissolve the material.
If water is the solvent, the first rinsing only must go into an appropriate waste container, the remaining rinses contain de minimus quantities of hazardous material and may go in the sink with the water running.
If a non-aqueous solvent is used, all rinses must go into an appropriate waste container and the rinsed container placed in a fume hood to allow remaining vapors to be drawn up the hood.Decontaminated items can go in non-hazardous trash.
Identify amounts of waste anticipated and appropriate disposal procedures. Segregate waste by hazard class (flammable, corrosive, etc.), state (liquid, solid) and, for organic solvents halogenated and non-halogenated. Store waste appropriately for the hazard class. Contact the CHO or CAS Stockroom Manager if you need assistance.
General hazardous waste disposal guidelinesLabel Waste
Affix hazardous waste label on all waste containers as soon as the first drop of waste is added to the container.DO NOT share waste containers with other research groups. Each research group shall maintain
their ownwaste containers for their procedures.
Store WasteStore hazardous waste in closed containers, in secondary containment, and in your laboratory’s designated location. Waste containers MUST be closed at all times, except when waste is being added to the container.Waste containers are considered FULL when they contain approximately 75% of the maximum volume.DO NOT OVERFILL – this is an exposure hazard for all persons handling and disposing of the waste.
Dispose of WasteCall EHS or Lab Support (CAS only) to have full waste containers picked up for disposal by EHS.
Lab SOP Page 5 of 20 June 2017
Methods of Disposal ☐ Consumed during process☐ Neutralized as part of procedure☐ Sink disposal (GHS non-hazardous liquids only – MUST HAVE EHS approval)☐ Hazardous waste by EHS (will be lab-packed by a Hazardous Waste Management company if cannot go to a sanitary landfill; associated disposal costs may be assessed to your research or department budget)
Chemical specific instructions: Click here to enter text. Describe the measures to take, procedures to follow for proper disposal or packaging of chemical waste.
#9 Training RequirementsPrior to conducting any work in this SOP, the PI or designee must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination and emergency procedures.The PI must provide or make readily available a copy of this SOP and copies of SDSs provided by the manufacturer.The PI must ensure that his/her laboratory personnel have attended appropriate and required laboratory safety training or refresher training within the last 12 months.
Lab-Specific TrainingClick here to enter text. List any additional training required before laboratory personnel can begin work using this SOP.
Location of SDS(s)UAA uses a web-based chemical inventory system, CISPro, which can be viewed anywhere an internet connection is available. CISPro can be viewed using Internet Explorer, Mozilla Firefox and Google Chrome. If your browser does not permit access to CISPro, please use one listed.
SDS(s) are available without a password at:https://cisprolive1.chemswlive.com/CISPro/login_msds.asp?error=&accessid=1664&netlogon=clear
PI’s have a secure login to view their chemical inventory and SDSs on CISPro and may choose to make their login and password available to their laboratory personnel. The password is reset at the beginning of each fiscal year (on or about July 1) and the information emailed to the PI. CISPro is accessed at:www.accelrys.com
If the PI is locked out or forgets his/her password, please contact either the UAA CHO or Lab Support Stockroom/ Hazardous Waste Manager for assistance.
#10 ApprovalsIn accordance with EHSRMSEM, I accept the responsibility for the proper use and disposal of these materials in the laboratory work described above and have assigned chemical safety responsibilities within the laboratory to people with appropriate training and/or experience.
Lab SOP Page 6 of 20 June 2017
I have reviewed and approve this Standard Operating Procedure.
Click here to enter a date.
PI Signature
Click here to enter a date.
EHSRMSEM Approval
#11 Emergency Phone NumbersPrinciple Investigator Click here to enter text. Click here to enter text.
EHSRSMEM CHO Marcy Marino 907-786-1279 or 716-201-8051ESHRMSEM Director Doug Markussen 907-786-1335Director of CAS Facility Support Krystal Haase 907-786-1264Radiation Safety Officer John Moore 907-786-1268 or 907-830-0669Building Manager Click here to enter text. Click here to enter text.
EHSRMSEM Safety Officer Kelly Carothers 907-786-1178ALL EMERGENCIES 911 systems – APD, AFD, UPD 911UPD – Non-emergency Dispatch 907-786-1120
Lab SOP Page 7 of 20 June 2017
#12 Documentation of Training and ProficiencyAdd additional lines to the table as necessary.The PI’s initials indicate the below listed persons have been trained and are proficient in this SOP.
I have read and understand the content of this SOP.
Name - Print Signature Date PI’s Initials
Lab SOP Page 8 of 20 June 2017
Appendix A
Risk Maps Guide Mitigation Efforts
Cons
eque
nces
Sign
ifica
nt
Manage and Monitor Risks (3)
Highest Risk Management and Monitoring (4)
Highest Risk Management and Monitoring (5)
Mod
erat
e
Acceptable Risks with Monitoring (2)
Manage and Monitor Risks (3)
Highest Risk Management and Monitoring (4)
Min
or
Acceptable Risks (1) Acceptable Risks with Monitoring (2)
Manage and Monitor Risks (3)
Low/Remote Moderate High/Certain
Likelihood
Table Legend
Low Hazard ProtocolModerate Hazard ProtocolSignificant Hazard ProtocolExtreme Hazard Protocol
For Numbers (1), (2), (3), (4), (5) see Assessing Risks: Consequences – on the next page
Lab SOP Page 9 of 20 June 2017
Assessing Risks: Consequences
Service Disruption Affects Funding &
ProcessesReputation Failure to Meet
Legal Obligations People
5Extreme
Total failure of service, extremely
expensive $$$$
National publicity >3 days
Resignations
Multiple Criminal/ Civil Suits, Claims
or Fines >$5M
Fatality of 1+ employees or
citizens
4Very High
Serious disruption to service, costly $
$$
National public and press interest
and coverage
Litigation, Claim or Fine of
$500K-5M
Serious injury/ disability of 1+
people
3Medium
Disruption to service,
moderately costly$$
Local public and press interest and
coverage
Litigation, Claim or Fine of
$100K-500K
Major injury to people
2Low
Some minor impact on service,
minor cost$
Contained within department by
known by entity
Litigation, Claim or Fine of
$10K-100K
Minor injuries to people
1Negligible
Annoyance, small or no impact on service and costs
Contained within department
Litigation, Claim or Fine of<$10K
Minor injury to an individual
Lab SOP Page 10 of 20 June 2017
Assessing Risks: Likelihood
1 or fewer Persons
1-10 Persons 10-30 Persons 30-90 Persons 90+
Persons
Extreme70-90%
Fatality of 1+ employees or
citizens
Very High50-70%
Serious injury/ disability of 1+
people
Medium30-50%
Major injury to people
Low10-30%
Minor injury to people
Negligible0-10%
Minor injury to an individual
Table Legend
Low Hazard ProtocolModerate Hazard ProtocolSignificant Hazard ProtocolExtreme Hazard Protocol
Lab SOP Page 11 of 20 June 2017
Appendix B
Biological Material Hazard Assessment FormComplete and attach a Biological Material Assessment form to the SOP. One form may be used for all
materials/agents with the same potential hazards and protective measures.
Biological Agent(s): Click here to enter text.(Organisms, Tissues, Fluids, etc.)
#1 Hazard IdentificationIdentify the hazard of the agent(s) listed above:Micro-organisms ☐ BSL1 ☐ BSL2Body fluids or tissues ☐ Animal ☐ Human
Modes of Exposure/Transmission – check all that apply☐ Inhalation ☐ Ingestion ☐ Mucous membranes ☐ Percutaneous (cut, needlestick, etc.) ☐ Skin contact
#2 Bloodborne PathogenThe Bloodborne Pathogens Standard (29 CFR, Bloodborne Pathogens. - 1910.1030) applies to all occupational exposure to blood or other potentially infectious materials. Blood means human blood, human blood components, and products made from human blood. Bloodborne Pathogens means pathogenic microorganisms that are present in human blood and can cause disease in humans. These pathogens include, but are not limited to, hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV). Additionally, “Other Potentially Infectious Materials” (OPIM) are included under this standard. OPIM means (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ, including cells and cell lines, (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV, HBV or HCV. The above additionally applies to non-human primate materials.
To help determine if a worker is at risk for contact with BBP, please check all that apply.Will any lab personnel:☐ Work with human blood, blood products or body fluids?
☐ Work with unfixed human cells (including tissue culture cells and cell lines), human tissues or organs?
☐ Work with non-human primates (NHP) or NHP blood, blood products or body fluids?
☐ Work with unfixed NHP cells (including tissue culture cells and cell lines), NHP tissues or organs?
☐ Work with bloodborne pathogens (e.g. HIV, Hepatitis B, Hepatitis C or other infectious agents able to be spread via blood)?Lab SOP 12 of 20 created December 2016
☐ Work with animals or animal tissues that have been infected with a BBP?
☐ Perform tasks which may potentially result in exposure to human or animal blood, body fluids, organs, or tissues which are infected with the hepatitis B virus or other bloodborne pathogens?
☐ Handle sharp instruments such as knives, needles, scalpels, or scissors which have been used by others working with human blood or other potentially infectious materials to include human organs, tissue or body fluids OR used by others working with similar body parts and fluids from animals infected with the hepatitis B virus or other bloodborne pathogens?
If ANY of the above questions are checked, then the worker is considered to be at occupational risk of contracting Hepatitis B or other bloodborne pathogens. All workers at risk MUST take the Bloodborne Pathogen Training. Contact EHS for training.
#3 Medical ConsiderationsDescribe what medical screening and surveillance is necessary, including any recommended vaccinations.If an individual declines recommended HBV vaccine, the Vaccination Declination form must be signed.
Click here to enter text.
#4 Safety Controls and EquipmentEngineering Controls – check all that apply ☐ Biosafety Cabinet
☐ Other: Click here to enter text. Specify other engineering controls required.
Personal Protective Equipment (PPE) – check all that applyAll recommended PPE is based on the most severe/ extreme conditions of exposure. PPE may be scaled back based on actual working quantities and/ or conditions.
MANDATORY - Appropriate clothing. See Chemical Hygiene Plan for more information.
Eye Protection – Safety glasses/ goggles must have ANSI stamp and fit properly; goggles should be snug enough to prevent any splash from reaching the eyes.
☐ Safety glasses ☐ Splash-proof goggles☐ Face shield (only in addition to glasses or goggles, NEVER as the sole source of eye protection)
Body protection – An appropriately-sized lab coat must be worn and fastened. Laboratory coat sleeves must be of sufficient length to prevent direct skin exposure while wearing gloves.
☐ Lab Coat ☐ ApronClick here to enter text. Specify any special instructions for decontamination and/ or care of reusable body protection.
Lab SOP 13 of 20 created December 2016
☐ Disposable Lab CoatClick here to enter text. Specify any special instructions for use and disposal of disposable body protection.
Hand protection – Wear chemical-resistant gloves. REMOVE DISPOSABLE GLOVES EVERY TIME THEY BECOME CONTAMINATED OR YOU LEAVE THE LAB. WASH YOUR HANDS EVERY TIME YOU REMOVE YOUR GLOVES.
Gloves ☐ Single glove ☐ Double glove
Thickness Click here to enter text. Length ☐ Standard ☐ Long
Material ☐ Butyl ☐ Latex ☐ Neoprene ☐ Nitrile☐ Thermal protection for: ☐ Heat ☐ Cold☐ Other Click here to enter text. Specify SDS recommended glove material.
Respiratory protection is generally not required for laboratory research, provided the appropriate engineering controls are employed. For additional guidance on respiratory protection, contact EHS.
#5 Cleaning and DisinfectionList and describe laboratory decontaminates and disinfectants that are used. List all applications and the decontaminate or disinfecting procedures with regards to the instrument or surface, agent used, frequency of agent preparation, allowed contact time before wiping and frequency of decontamination/ disinfection. Click here to enter text.
#6 Waste Generation and DisposalIdentify the types of waste generated and procedures for handling biological waste including contaminated and non-contaminated waste and use of sharps.Click here to enter text.
#7 Spill ResponseDescribe protocol for cleaning up spills, maintaining staff safety, decontamination and disposal.Small/ manageable spillsProtect yourself
Wear PPEContain the spill
Notify others in the lab and keep people away from the areaDisinfect the spilled material
Cover the spill with paper towels or spill padsPour (do not spray) freshly made bleach or other appropriate disinfectant onto the paper towels (working from the outside inward)Allow to sit for 20 minutes
Clean upSweep up materials and place into a biohazard bagDo not pick up sharps or broken glass with your hands; use tongs, dust pan and broom or anotheravailable materialDispose of sharps in a sharps container
Lab SOP 14 of 20 created December 2016
Dispose of broken glass in a broken glass container – DO NOT put other materials in the broken glass containerDispose of PPR in bio-waste container
Large/ unmanageable spillsProtect yourself and othersNotify everyone in the immediate areaCall EHSRMS&EM for assistance
Lab specific protocolsClick or tap here to enter text. Describe any lab specific protocols for managing spills.
#8 Accident/ Exposure ResponseAre there first aid or emergency response procedures necessary for this material beyond rinsing with water? ☐No☐Yes, specify in the Emergency Procedures section of the SOP.
For information only. See HR for actual declination form
29 CFR 1910.1030 Appendix A
Hepatitis B Vaccination Declination
I understand that due to my occupational exposure to blood or other potentially infectious materials I may be at risk of acquiring hepatitis B virus (HBV) infection. I have been given the opportunity to be vaccinated with hepatitis B vaccine, at no charge to myself. However, I decline hepatitis B vaccination at this time. I understand that by declining this vaccine, I continue to be at risk of acquiring hepatitis B, a serious disease. If in the future I continue to have occupational exposure to blood or other potentially infectious materials and I want to be vaccinated with hepatitis B vaccine, I can receive the vaccination series at no charge to me.
Lab SOP 15 of 20 created December 2016
Appendix C
Chemical Hazard Assessment FormComplete and attach a Chemical Hazard Assessment form for EACH hazardous chemical listed in the SOP.
Chemical/ Reagent: Click here to enter text.CAS#: Click here to enter text.
#1 Hazards – check all that apply.☐ Acute toxicity ☐ Explosive ☐ Respiratory sensitizer☐ Aspiration hazard ☐ Flammable ☐ Self-heating☐ Asphyxiant ☐ Gas under pressure ☐ Self-reactive☐ Carcinogen ☐ Germ cell mutagen ☐ Skin - corrosive☐ Corrosive to metal ☐ Organic peroxide ☐ Skin - irritant☐ Combustible dust ☐ Oxidizer ☐ Skin - sensitizer☐ Eye - irritation ☐ Pyrophoric ☐ Specific target organ toxicity (STOT)☐ Eye – serious damage ☐ Reproductive toxin ☐ Water-reactive☐ Other: Click here to enter text. Hazards not otherwise specified.
Maximum quantity to be used or stored in the next 12 months.☐ < 1L or 100 g ☐ 1 L or 100 g to 4L or 1 kg ☐ > 4L or 1 kg
Chemical state and concentration to be used☐ Solid ☐ Gas☐ Liquid or solution ☐ Dilute (<5%) ☐ Intermediate (5-25%) ☐ Concentrated (>25%)
Where will this material be stored?☐ General storage (cabinet, shelf) ☐ Flammable cabinet ☐ Locked cabinet (toxins)
☐ Corrosive cabinet ☐ Household refrigerator-freezer☐ Flammable-rated refrigerator-freezer
Incompatibles and reactives: Click here to enter text. List ALL chemicals, conditions and/or materials that this chemical should not come in contact with. Information can be found on the SDS and in CISPro
Secondary containment required for storage? (UAA CHP strongly recommends secondary containment for corrosive (acid, base) and flammable solvent bottles larger than 500mL.)
☐ No ☐ Yes – obtain containment compatible with chemical
Significant Hazard Protocol
Lab SOP 16 of 20 created December 2016
(UAA Laboratory Access Policy states ‘Individuals engaging in Significant Hazard Protocols must…place a “Protocol in Process” sign on all entry doors to the lab). Is this chemical used in a Significant Hazard Protocol?
☐ No ☐ Yes – see Lab Access Policy for ‘Protocol in Process’ form
#2 Safety Controls and EquipmentEngineering Controls
This chemical must be used with the following (check all that apply)☐ Chemical fume hood ☐ Biosafety cabinet☐ Glove box ☐ Local exhaust (e.g. snorkel)☐ Other: Click here to enter text.
Does this chemical require use of a designated area other than specified above? ☐ Yes ☐ No(Carcinogens, reproductive hazards and highly acute toxins require a designated area)
If yes, describe the designated area: Click here to enter text.
Does the area of use need to be decontaminated after use? ☐ Yes ☐ No(Designated areas for carcinogens, reproductive hazards and highly acute toxins require decontamination)
If yes, describe the decontamination procedure: Click here to enter text.
Personal Protective Equipment (PPE)All recommended PPE is based on the most severe/ extreme conditions of exposure. PPE may be scaled back based on actual working quantities and/ or conditions.
MANDATORY - Appropriate clothing. See Chemical Hygiene Plan for more information.
Eye Protection – Safety glasses/ goggles must have ANSI stamp and fit properly; goggles should be snug enough to prevent any splash from reaching the eyes.
☐ Safety glasses ☐ Splash-proof goggles☐ Face shield (only in addition to glasses or goggles, NEVER as the sole source of eye protection)
Body protection – An appropriately-sized lab coat must be worn and fastened. Laboratory coat sleeves must be of sufficient length to prevent direct skin exposure while wearing gloves.
☐ Apron ☐ Lab Coat ☐ Flame-resistant Lab Coat☐ Other Click here to enter text. Specify SDS recommended item.
Hand protection – Wear chemical-resistant gloves. REMOVE DISPOSABLE GLOVES EVERY TIME THEY BECOME CONTAMINATED OR YOU LEAVE THE LAB. WASH YOUR HANDS EVERY TIME YOU REMOVE YOUR GLOVES.
Gloves ☐ Disposable ☐ Reusable
Thickness Click here to enter text. Length ☐ Standard ☐ Long
Lab SOP 17 of 20 created December 2016
Material ☐ Butyl ☐ Latex ☐ Neoprene ☐ Nitrile☐ Thermal protection for: ☐ Heat ☐ Cold☐ Other Click here to enter text. Specify SDS recommended glove material.
Respiratory protection is generally not required for laboratory research, provided the appropriate engineering controls are employed. For additional guidance on respiratory protection, contact EHS.
#3 Monitoring and Exposure AssessmentExposure Limits ☐ Not applicable (Air concentration of chemical gas or vapor, or fiber particulate. Consult chemical SDS, AKOSH Air Contaminant Table-Z-1-A, or NIOSH Pocket Guide for values.) Definitions of terms and abbreviationsAction Level -Level of air contaminant at or above which OSHA requires the employer to take actionCeiling – maximum level of air contaminant that should never be exceeded for any length of time.IDLH – Immediately Dangerous to Life and HealthPEL – Permissible Exposure Limit – TWA concentration of air contaminant that must not be exceeded during any 8-hour work shift of a 40-hour workweek. OSHA/ AKOSH termREL – Recommended Exposure Limit – TWA concentration of air contaminant that must not be exceeded during any 10-hour work shift of a 40-hour workweek. NIOSH termSTEL – Short Term Exposure Limit – a 15- minute TWA concentration of an air contaminant that must not be exceeded at any time during a workday,TWA – Time Weighted Average - employee’s exposure to a specific air contaminant averaged over an 8-hour workday.
Action level Click here to enter text.AKOSH PEL TWA: Click here to enter text. STEL: Click here to enter text. Ceiling: Click here to enter text.
NIOSH REL TWA: Click here to enter text. STEL: Click here to enter text. Ceiling: Click here to enter text.
IDLH Click here to enter text. Skin ☐ Check box if AKOSH Table or NIOSH guide indicate [skin] designation.
How will the exposure to this chemical be assessed? The OSHA Laboratory Standard requires that workers exposed to regulated hazardous chemicals, including but not limited to formaldehyde/ formalin, phenol, benzene, inorganic arsenic, and lead, be assessed before work begins and during laboratory operations if necessary, to ensure action levels, PELs and/ or STELs of regulated chemicals are not exceeded. Indicate how worker exposure will be assessed.
☐ Professional judgement of PI/RLS ☐ Area monitoring ☐ Personal monitoring (badging)
Is medical surveillance required for users of this material? OSHA requires medical surveillance for worker exposed to regulated hazardous chemicals, including but not limited to formaldehyde/ formalin, phenol, benzene, inorganic arsenic, and lead, at or above the action level or STEL. If yes or unsure, please contact EHS for further information.
☐ Yes ☐ No
#4 OSHA Required TrainingThe use of certain chemicals may require training above that required by the OSHA Lab Standard, 29 CFR 1910.1450. These chemicals
Lab SOP 18 of 20 created December 2016
have their own OSHA standards and training requirements which are found in Subpart Z, 1910.1000-1096.Does this chemical have its own OSHA standard?
☐ No ☐ Yes; Click here to enter text. Enter the OSHA standard If yes, is there an exposure limit below which additional training is not required?
☐ No ☐ Yes; Click here to enter text. Enter exposure limit.
#5 First Aid and Emergency ResponseAre there first aid or emergency response procedures necessary for this material beyond rinsing with water? ☐ No☐ Yes, specify in the Emergency Procedures section of the SOP.This section is for any emergency procedures different from standard responses, or for additional emergency information due to the nature of materials or task. Include information on personal exposure / medical emergency as appropriate (e.g. Calgonate gel for hydrofluoric acid exposure).
#6 Spill ResponseSmall/ manageable spills In the event of a minor spill or release that can be safely cleaned up using laboratory PPE and spill kit (Amphomag), spills or releases which have been contained within the laboratory (via secondary containment, in a fume hood, on a bench or the floor), spills of less than 500 mL of any flammable material, or spills of materials that do not require respiratory protection for cleanup:
Notify personnel in the area and keep people away from the area. Eliminate all sources of ignition.Protect yourself:
Review the SDS for the spilled material, or use your knowledge of the hazards of the material to determine the appropriate level of protection.
Wearing appropriate PPE, clean up the spill with Amphomag following the directions provided in the spill kit. Collect spill cleanup materials in a tightly closed container or double plastic bags. Manage spill cleanup debris as hazardous waste. Contact EHS for waste pickup.Dispose of broken glass in a broken glass container – DO NOT put other materials in the broken glass container.
DO NOT clean up spills requiring respiratory protection – CALL EHS.
Large/ unmanageable spillsFor highly reactive, highly acute toxin, spills or releases which have impacted the environment (via sink / sewer system, soil or air outside the building), spills larger than 500mL of any flammable material, or spill of any material requiring respiratory protection for cleanup:
Protect yourself and others:Contact PI/ RLS and EHS personnel on Emergency Phone Number list.Alert people in the vicinity of the spill, advise them to evacuate the immediate area.Restrict access to the area of the spill. Eliminate all sources of ignition on your way out.Evacuate to a safe area. Remain nearby to advise PI and EHS.
Lab-Specific (Biological/Chemical/Radiation Hazard specific) ProceduresThis section is for any emergency procedures different from standard responses, or for additional emergency
Lab SOP 19 of 20 created December 2016
information due to the nature of materials or task. Include information on gas leaks, chemical spills, etc.
#7 Waste Disposal and Pollution PreventionGeneral hazardous waste disposal guidelinesLabel Waste
Affix hazardous waste label on all waste containers as soon as the first drop of waste is added to the container.DO NOT share waste containers with other research groups. Each research group shall maintain their ownwaste containers for their procedures.
Store WasteStore hazardous waste in closed containers, in secondary containment, and in your laboratory’s designated location. Waste containers MUST be closed at all times, except when waste is being added tothe container.Waste containers are considered FULL when they contain approximately 75% of the maximum volume.DO NOT OVERFILL – this is an exposure hazard for all persons handling and disposing of the waste.
Dispose of WasteCall EHS or Lab Support (CAS only) to have full waste containers picked up for disposal by EHS.
Methods of Disposal ☐ Consumed during process☐ Neutralized as part of procedure☐ Sink disposal (GHS non-hazardous liquids only – MUST HAVE EHS approval)☐ Hazardous waste by EHS (will be lab-packed by a Hazardous Waste Management company if cannot go to a sanitary landfill; associated disposal costs may be assessed to your research or department budget)
Chemical specific instructions: Click here to enter text. Describe the measures to take, procedures to follow for proper disposal or packaging of chemical waste.
Lab SOP 20 of 20 created December 2016
