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International Federation forConsulting GmbH IFC

Method Validation Method Validation and QC for Pesticide and QC for Pesticide

Residue Analysis Residue Analysis SANCO/2007/3131SANCO/2007/3131

by

Dr. Michael Scheutwinkel

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Outline

- This SANCO paper applies for all governmental laboratories and those private ones working in the official food control.

- Accreditation Bodies are using this paper as basis for validation and quality control.

SANCO/2007/3131

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Calibration….- representative analytes for each bach of analyses- all substances at least every 3 months

At least 15 analytes + 25% of total number of analytes(100 analytes = 40 components)

At least 15 analytes + 25% of total number of analytes(100 analytes = 40 components)

SANCO/2007/3131

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Calibration….

- Matrix matched …- Grouping of food stuff …

The most effective way to negate matrix effects is tocalibrate with standard addition.

The most effective way to negate matrix effects is tocalibrate with standard addition.

SANCO/2007/3131

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Validation Model

List of all pesticides

analysed in routine

List of all pesticides

analysed in routine

RepeatabilityRepeatabilityRecoveries,70 – 120 %

Recoveries,70 – 120 %

LOD‘s /LOQ in different types

of matrix reproducibiity

LOD‘s /LOQ in different types

of matrix reproducibiity

Reproducibility Reproducibility

Pesticide conceptPesticide concept

Which pesticide is detected

how?

Which pesticide is detected

how?

Fulfilment of legal requirementsFulfilment of legal requirements