1 Faculty Advisors: Steven Hall, Ph. D., Department of Biological and Agricultural Engineering...
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1 Faculty Advisors: Steven Hall, Ph. D., Department of Biological and Agricultural Engineering Charles Malveaux, Department of Biological and Agricultural Engineering Brent Christner, Ph. D., Department of Biological Sciences Noelle Bryan, Department of Biological Sciences Student Investigators: Samuel Beck, Bradley Bernard, Rheagan Chambers, Kristen Galloway, Brandon Landry, Alexandra Williams
1 Faculty Advisors: Steven Hall, Ph. D., Department of Biological and Agricultural Engineering Charles Malveaux, Department of Biological and Agricultural
1 Faculty Advisors: Steven Hall, Ph. D., Department of
Biological and Agricultural Engineering Charles Malveaux,
Department of Biological and Agricultural Engineering Brent
Christner, Ph. D., Department of Biological Sciences Noelle Bryan,
Department of Biological Sciences Student Investigators: Samuel
Beck, Bradley Bernard, Rheagan Chambers, Kristen Galloway, Brandon
Landry, Alexandra Williams
Slide 2
Background Bio-precipitation Ice nucleating bacteria $ 1
billion in crop loss due to frost damage in US Current methods
HASP, Ice core, Hailstones 2 (Christner, Brent C., 2012) Background
Prob. Statement Objectives HoQ PDS Decomp. Function Diagram Morph.
Chart Design
Slide 3
Problem Statement To develop an efficient device that will
collect microbes in order to increase bacterial data at known
altitudes satisfying Federal Aviation Administration (FAA)
regulations. This will be accomplished by mounting the ACROBE to an
Unmanned Aerial System-Hexacopter (UAS-H). 3 Background Prob.
Statement Objectives HoQ PDS Decomp. Function Diagram Morph. Chart
Design
Slide 4
Measurable Objectives Collect 1m 3 of air Collect within 2m of
specified altitude 80% viability Weigh less than 10lbs 4 Background
Prob. Statement Objectives HoQ PDS Decomp. Function Diagram Morph.
Chart Design
Slide 5
House of Quality 5 Background Prob. Statement Objectives HoQ
PDS Decomp. Function Diagram Morph. Chart Design
Slide 6
Product Design Specification Construction of device will
include carbon fiber Assembly will be modular where possible to aid
in repair and disassembly The device will be designed for
reproducibility and reliability Operation vacuum pressure is
approximately 0.03 psi g (206.84 Pa g ) Operation between 40 F and
100 F (4.44C and 37.78 C) Sampling Time: 2.5-20 minutes 70% ethanol
and 2000 ppm NaHClO for cleaning Compliance with FAA Regulations 6
Background Prob. Statement Objectives HoQ PDS Decomp. Function
Diagram Morph. Chart Design
Detailed Function Structure Detailed Function Structure Diagram
8 Background Prob. Statement Objectives HoQ PDS Decomp. Function
Diagram Morph. Chart Design
Slide 9
Concept Generation Morphological Chart Morphological Chart 9
CollectionPlatformData LoggingBacterial Extraction P SourceCleaning
ImpactUAS-AManualDrainageVacuum Pump Autoclave
ImpingementUAS-HRemote + Automated SwabbingInducted Fan 70 %
Ethanol Air SamplingBalloonNoneStampingPropellerEthylene Oxide Ice
CoreAircraftAutomatedPipettingTurbineBoiling Water
HailstoneRocketAgitationNaHClO Dry heat Background Prob. Statement
Objectives HoQ PDS Decomp. Function Diagram Morph. Chart
Design
Slide 10
Impactor Design 10 Impact Calculations Efficiency Factors
Control Systems Materials Fluid Dynamics Assembly Design
Slide 11
Calculations 11 (Yao and Mainelis, 2007) Impact Calculations
Efficiency Factors Control Systems Materials Fluid Dynamics
Assembly Design
Slide 12
Calculations (Cont.) 12 Impact Calculations Efficiency Factors
Control Systems Materials Fluid Dynamics Assembly Design
Slide 13
Design-Efficiency Factors 13 Marple and Willekes Method (Marple
and Willeke, 1976) Impact Calculations Efficiency Factors Control
Systems Materials Fluid Dynamics Assembly Design
Slide 14
Impact Smart Control System Arduino platform with ATmega2560
microcontroller Pressure, temperature, humidity, and wind direction
sensor Out-runner brushless DC motor drives vacuum Three servos to
open and close inlets and outlet SD card shield for data
acquisition 14 (ArduinoBoardMega, 2014) (New Generation Hobbies,
2014) (Sparkfun) Calculations Efficiency Factors Control Systems
Materials Fluid Dynamics Assembly Design (Mouser Electronics,
2014)
Slide 15
Negative Feedback Pressure sensor will determine motors speed
Wind direction will determine where ACROBE inlets face 15 Impact
Calculations Efficiency Factors Control Systems Materials Fluid
Dynamics Assembly Design
Slide 16
Arduino Circuit Schematic 16 Impact Calculations Efficiency
Factors Control Systems Materials Fluid Dynamics Assembly
Design
Fluid Dynamics Autodesk Simulation CFD Autodesk Simulation CFD
19 Impact Calculations Efficiency Factors Control Systems Materials
Fluid Dynamics Assembly Design
Slide 20
AutoDesk CFD Data 20 Impact Calculations Efficiency Factors
Control Systems Materials Fluid Dynamics Assembly Design
Slide 21
ACROBEACROBE-Hexacopter Assembly 21 Impact Calculations
Efficiency Factors Control Systems Materials Fluid Dynamics
Assembly Design
Slide 22
Budget ItemsPrice Epoxy$15.99 PVC Pipe$24.07 Clear Lexan$12.72
Polycarbonate Tubing$137.91 Vacuum and Fuel Pressure Kit$17.84
Temperature and Humidity Sensor$29.95 ABS Pressure Sensor(x3)$48.27
TOTAL $286.75/$1100.00 22 Budget Bill of MaterialsGantt Chart
Conclusion References
Slide 23
Bill of Materials 23 Budget Bill of MaterialsGantt Chart
Conclusion References ItemQuantityCombined Price MPX4115a3$48.27
HIH6130 Sparkfun1$29.95 Arduino Mega1$37.52 EMS22P50-B28-LS61$37.80
SD Card Shield Adafruit1$19.95 Futaba ASD Sensor1$179.97 Futaba
BLS1533$360.00 7.4 V 2S LiPo 1200 mAh1$16.99 Futaba R7008SB S.Bus2
2.4GHz FASSTest Rx1$139.98 CF Rod 1 x 601$75.99 CF Rod.686 x
721$55.99 CF Rod.1 x 242$4.90 West Systems 105/206 Epoxy1$62.61
West Systems 423 Graphite Powder1$19.03
Slide 24
Bill of Materials 24 Budget Bill of MaterialsGantt Chart
Conclusion References ItemQuantityCombined Price CF Plate 300mm x
100mm x 2mm2$39.99 Frekote 7001$20.50 Neoprene w/ Adhesive 54 x 1
x.1251$20.00 6061-T6 Aluminum 4 x 4 x.1252$23.66 Du-Bro 993 Nylon
Bolt 3 x.251$2.36 Aluminum Foil 18 x 25 x.0011$16.95 Teknova R2A
Broth Premix (500 g)1$176.57 Growcells MRGF-6235-12 10X PBS (12
L)1$292.78 2,000ppm NaHClO (1 L)1 - SEOH R2A Agar Media (500
g)1$75.52 20 pk. Petri Dishes 100mm x 13mm10$79.50 Carbon Fiber
Fabric 3K 50 x 361$50.85 Heathrow Scientific HS86655 Stainless
Steel Large Bacterial Cell Spreader 1$14.76 Total: $724.28/
$1906.67
Slide 25
Gantt Chart 25 Budget Bill of MaterialsGantt Chart Conclusion
References
Slide 26
Conclusion 26 Ergonomic, easy to use design Efficient
collection with high repeatability/reproducibility Multiple runs in
a day Fully automated Data collection First of its kind Budget Bill
of MaterialsGantt Chart Conclusion References
Slide 27
"6061 Aluminum." 6061 Aluminum, Aluminium, Alumina Bobco Metals
LLC. Bob Co. Metals, 2014. Web. 02 Dec. 2014. "Air - Altitude,
Density and Specific Volume." Air - Altitude, Density and Specific
Volume. Engineering Toolbox, n.d. Web. 02 Dec. 2014. "Arduino -
ArduinoBoardMega." Arduino - ArduinoBoardMega. N.p., 2014. Web. 01
Dec. 2014. Carbon Fiber Fabric." 3K Plain Weave Carbon Fiber. The
Composite Store (CST), 2014. Web. 02 Dec. 2014. Christner, Brent C.
"Cloudy with a Chance of Microbes." News Magazine of the American
Society for Microbiology Feb. 2012: 70-75. Web. 20 Nov. 2014.
"Darcy-Weisbach Equation History." Biosystems-OK State. Oklahoma
State, 15 June 2000. Web. 01 Dec. 2014. "EMS22P50-B28-LS6 Bourns |
Mouser." Mouser Electronics. Mouser Electronics, 2014. Web. 01 Dec.
2014. "Gold Streak Plate -- Petri Dish Coasters." Flickr. Yahoo!,
n.d. Web. 22 Nov. 2014. Journal of Microbiological Methods, Volume
107, December 2014, Pages 161-168 N.C. Bryan, M. Stewart, D.
Granger, T.G. Guzik, B.C. Christner Marple, Virgil A., and Klaus
Willeke. "Impactor Design." Atmospheric Environment 10 (1976): 891-
96. University of North Carolina. Pergamon Press. Web. 20 Nov. 2014
"Microscope Use in Brewing." Microscope Use. N.p., 7 Oct. 2012.
Web. 22 Nov. 2014 New Generation Hobbies. MPX4115A Pressure Sensor,
2014. Web. 1 Dec. 2014. "Nylon Nuts, Bolts & Washers."
IndustrySearch. Industry Search, 2014. Web. 02 Dec. 2014. "SparkFun
Humidity and Temperature Sensor Breakout - HIH6130." - SEN-11295.
Sparkfun, n.d. Web. 01 Dec. 2014. "West Systems Laminating Epoxy
Resin." West System Epoxy. The Composite Store (CTS), 2014. Web. 02
Dec. 2014. What Can I Do With My Model Aircraft?"
Hobby/Recreational Flying. Federal Aviation Administration, 12 Aug.
2014. Web. 19 Nov. 2014. Whyte, W., G. Green, and A. Albisu.
"Collection Efficiency and Design of Microbial Air Samplers."
Journal of Aerosol Science 38.1 (2007): 97 110.ScienceDirect. Web.
15 Sept. 2014 Yao, Maosheng, and Gediminas Mainelis. "Investigation
of Cut-Off Sizes and Collection Efficiencies of Portable Microbial
Samplers." Aerosol Science and Technology 40.8 (2007): 595-606.
Taylor and Francis Online. Taylor and Francis, 1 Feb. 2007. Web. 15
Nov. 2014. 27 Budget Bill of MaterialsGantt Chart Conclusion
References